Novipirellula artificiosorum

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Novipirellula

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7087 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6E2G2|A0A5C6E2G2_9BACT Uncharacterized protein OS=Novipirellula artificiosorum OX=2528016 GN=Poly41_12160 PE=4 SV=1
MM1 pKa = 7.42CLKK4 pKa = 10.47RR5 pKa = 11.84SRR7 pKa = 11.84SRR9 pKa = 11.84AWTTSEE15 pKa = 3.26WTATDD20 pKa = 4.11DD21 pKa = 4.98SATTDD26 pKa = 3.04EE27 pKa = 4.38DD28 pKa = 4.07TAVVMNVIANDD39 pKa = 3.73SDD41 pKa = 4.34PDD43 pKa = 4.18GDD45 pKa = 4.17SLLVDD50 pKa = 4.54SIGVAAHH57 pKa = 5.51GTIANNADD65 pKa = 3.17GTITYY70 pKa = 8.97TPDD73 pKa = 3.09ANYY76 pKa = 10.23HH77 pKa = 5.88GSDD80 pKa = 3.08SFTYY84 pKa = 10.16TLADD88 pKa = 3.54GNGGVDD94 pKa = 3.18TGTVMVSVTPVNDD107 pKa = 3.31APVAVDD113 pKa = 3.7DD114 pKa = 5.65DD115 pKa = 4.69YY116 pKa = 12.31VVDD119 pKa = 3.74QDD121 pKa = 3.55MSLVVPASGVLANDD135 pKa = 3.6SDD137 pKa = 3.98VDD139 pKa = 4.12ADD141 pKa = 4.0TLSAAIVGDD150 pKa = 4.08PSHH153 pKa = 6.94GDD155 pKa = 2.8VWLGEE160 pKa = 4.64DD161 pKa = 4.32GSFTYY166 pKa = 10.29TPEE169 pKa = 5.19DD170 pKa = 3.06GFYY173 pKa = 10.71GQDD176 pKa = 2.83SFTYY180 pKa = 9.79RR181 pKa = 11.84VQDD184 pKa = 3.33SSLVSDD190 pKa = 4.25TATVTIMVNRR200 pKa = 11.84VNSVPVANDD209 pKa = 3.02DD210 pKa = 4.3SYY212 pKa = 12.1DD213 pKa = 3.52VDD215 pKa = 3.85EE216 pKa = 5.16DD217 pKa = 3.8QPINVATPGVLGNDD231 pKa = 3.83RR232 pKa = 11.84DD233 pKa = 4.28DD234 pKa = 5.34DD235 pKa = 4.72GDD237 pKa = 5.1PITANIISGVSHH249 pKa = 6.45GQLNLNQDD257 pKa = 2.88GSFNYY262 pKa = 9.67TPEE265 pKa = 4.22ANFAGTDD272 pKa = 3.47SFSYY276 pKa = 9.98VANDD280 pKa = 3.45GFGNSDD286 pKa = 3.32QAIVTLTVNSVNDD299 pKa = 3.35VPTAEE304 pKa = 4.33SQSIVLPEE312 pKa = 5.11DD313 pKa = 3.37DD314 pKa = 4.79SKK316 pKa = 11.53PVTLQGSDD324 pKa = 3.73LDD326 pKa = 4.82GDD328 pKa = 4.08TLTYY332 pKa = 9.95TVTIGPTHH340 pKa = 6.06GTLAGTAPALTYY352 pKa = 10.5EE353 pKa = 4.15PAANYY358 pKa = 10.38SGLDD362 pKa = 3.18SFTFTVSDD370 pKa = 5.26GIAEE374 pKa = 4.2SAAATVSISVTSVNDD389 pKa = 3.66APILNQPIDD398 pKa = 4.21DD399 pKa = 4.73LDD401 pKa = 3.86VTEE404 pKa = 5.74DD405 pKa = 3.66SADD408 pKa = 3.62SVIDD412 pKa = 3.64LTGMFSDD419 pKa = 4.53IDD421 pKa = 3.84ASDD424 pKa = 4.08LVTLSVQANSNSSLVTASLLDD445 pKa = 3.61NVLTLDD451 pKa = 3.91YY452 pKa = 11.2QPDD455 pKa = 3.7QNGTATVTIRR465 pKa = 11.84GTDD468 pKa = 3.19GSGAYY473 pKa = 9.87AQDD476 pKa = 3.57SFLVTVTAVNDD487 pKa = 3.58APVAVDD493 pKa = 5.04DD494 pKa = 5.16LANTPMDD501 pKa = 3.9TPVVIDD507 pKa = 4.07VLDD510 pKa = 3.93NDD512 pKa = 4.11TDD514 pKa = 3.63VDD516 pKa = 4.27GEE518 pKa = 4.44SLSIAIIGSTNGTAVVNEE536 pKa = 4.1NGTVSFTPSASFSGLASFTYY556 pKa = 9.63TLNDD560 pKa = 3.61GEE562 pKa = 4.83LDD564 pKa = 3.98SNVATVTIDD573 pKa = 3.27VTPIASGPKK582 pKa = 8.56LAHH585 pKa = 5.74GVVTGVGSSGWTTVVLSHH603 pKa = 7.01TYY605 pKa = 11.51DD606 pKa = 3.39NMVVIATPNYY616 pKa = 9.94DD617 pKa = 3.28SSDD620 pKa = 3.6APGVTRR626 pKa = 11.84IQNASGNQFDD636 pKa = 4.36VRR638 pKa = 11.84VDD640 pKa = 3.59SAGGGSLSDD649 pKa = 3.4VRR651 pKa = 11.84VHH653 pKa = 5.44YY654 pKa = 10.53VVVEE658 pKa = 3.82AGFYY662 pKa = 10.64DD663 pKa = 3.7EE664 pKa = 5.66PGYY667 pKa = 11.33KK668 pKa = 8.83MEE670 pKa = 4.91AVTFNSTRR678 pKa = 11.84TDD680 pKa = 3.49EE681 pKa = 4.26NNSWVGEE688 pKa = 4.16SRR690 pKa = 11.84SYY692 pKa = 10.06LQSYY696 pKa = 7.18SQPVVLGQVMSYY708 pKa = 11.16NDD710 pKa = 4.34PDD712 pKa = 3.44WSVFWASGSSRR723 pKa = 11.84SAAPTSSALTVGKK736 pKa = 9.69HH737 pKa = 5.14VGEE740 pKa = 5.13DD741 pKa = 3.45SDD743 pKa = 3.79NTRR746 pKa = 11.84SDD748 pKa = 3.17EE749 pKa = 4.09TLGYY753 pKa = 9.95IVIEE757 pKa = 4.2TSTTGTAEE765 pKa = 4.07IEE767 pKa = 4.13GLRR770 pKa = 11.84YY771 pKa = 9.26VAALGGDD778 pKa = 4.41SIRR781 pKa = 11.84GVGDD785 pKa = 3.5SPAYY789 pKa = 9.91SYY791 pKa = 11.26SYY793 pKa = 10.83QAMPNSKK800 pKa = 8.06TAVASQAAMDD810 pKa = 4.42GGNGGWAVLYY820 pKa = 10.67GNDD823 pKa = 4.84PITPTGNTINFAIDD837 pKa = 3.04EE838 pKa = 4.41DD839 pKa = 3.94QARR842 pKa = 11.84DD843 pKa = 3.42TEE845 pKa = 4.71RR846 pKa = 11.84YY847 pKa = 7.4HH848 pKa = 5.07TTEE851 pKa = 3.42QVAYY855 pKa = 8.88FIIDD859 pKa = 3.57PPVEE863 pKa = 3.98EE864 pKa = 4.75LRR866 pKa = 11.84ASVTAMDD873 pKa = 3.87ASGDD877 pKa = 3.73GRR879 pKa = 11.84VTSMDD884 pKa = 3.26ALLVINHH891 pKa = 6.97LNTNRR896 pKa = 11.84SHH898 pKa = 7.64DD899 pKa = 4.16NPSHH903 pKa = 7.29LDD905 pKa = 3.57LSQDD909 pKa = 3.61GAVTPLDD916 pKa = 3.47ALVTINHH923 pKa = 6.67LNQRR927 pKa = 11.84TRR929 pKa = 11.84SAIQSRR935 pKa = 11.84SPITPQAVDD944 pKa = 3.18QWWAMDD950 pKa = 5.05DD951 pKa = 3.67EE952 pKa = 6.42DD953 pKa = 6.03DD954 pKa = 5.11NEE956 pKa = 4.74GSMDD960 pKa = 3.8EE961 pKa = 4.4ALLDD965 pKa = 3.66ILISS969 pKa = 3.64

Molecular weight: 101.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6D2F9|A0A5C6D2F9_9BACT OPT oligopeptide transporter protein OS=Novipirellula artificiosorum OX=2528016 GN=Poly41_64890 PE=4 SV=1
MM1 pKa = 7.38GFRR4 pKa = 11.84RR5 pKa = 11.84FRR7 pKa = 11.84LDD9 pKa = 2.87VVTRR13 pKa = 11.84LGVLVFSLSLSFLGTKK29 pKa = 9.4TSVVFVVLGMGFMQGMFSILSAVTWPRR56 pKa = 11.84FFGRR60 pKa = 11.84SHH62 pKa = 6.88LGAVSGMSTSIVVVGTAISRR82 pKa = 11.84RR83 pKa = 11.84SRR85 pKa = 3.2

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7087

0

7087

2704480

29

11978

381.6

42.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.392 ± 0.031

1.185 ± 0.015

6.361 ± 0.037

6.015 ± 0.03

3.73 ± 0.018

7.67 ± 0.034

2.235 ± 0.018

5.084 ± 0.018

3.676 ± 0.032

9.439 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.019

3.29 ± 0.026

5.171 ± 0.026

4.044 ± 0.022

6.592 ± 0.044

6.721 ± 0.025

5.718 ± 0.056

7.228 ± 0.027

1.583 ± 0.017

2.532 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski