Streptomyces sp. wa22

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q4SH90|A0A5Q4SH90_9ACTN ABC transporter permease OS=Streptomyces sp. wa22 OX=1828244 GN=EAO68_26375 PE=3 SV=1
MM1 pKa = 7.46TVQQDD6 pKa = 3.52APGGGDD12 pKa = 3.48TQDD15 pKa = 3.55LEE17 pKa = 4.23VWIDD21 pKa = 3.38QDD23 pKa = 3.99LCTGDD28 pKa = 4.65GICVQYY34 pKa = 10.96APEE37 pKa = 4.18VFEE40 pKa = 5.9LDD42 pKa = 3.05IDD44 pKa = 3.63GLAYY48 pKa = 10.49VKK50 pKa = 10.6SDD52 pKa = 3.8EE53 pKa = 5.87DD54 pKa = 4.37EE55 pKa = 4.33LLQDD59 pKa = 4.37KK60 pKa = 10.59GATTPVPLPLLQDD73 pKa = 3.66VVDD76 pKa = 4.35SAKK79 pKa = 10.25EE80 pKa = 3.92CPGDD84 pKa = 4.18CIHH87 pKa = 6.58VRR89 pKa = 11.84RR90 pKa = 11.84VSDD93 pKa = 3.44NVEE96 pKa = 4.07VYY98 pKa = 10.91GPDD101 pKa = 3.4AEE103 pKa = 4.31

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q4SPB2|A0A5Q4SPB2_9ACTN SLATT domain-containing protein OS=Streptomyces sp. wa22 OX=1828244 GN=EAO68_05095 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7000

0

7000

2235303

18

3953

319.3

34.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.319 ± 0.039

0.768 ± 0.007

6.066 ± 0.024

5.746 ± 0.029

2.748 ± 0.016

9.652 ± 0.027

2.274 ± 0.015

3.155 ± 0.018

2.171 ± 0.025

10.247 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.789 ± 0.011

1.781 ± 0.014

5.976 ± 0.025

2.714 ± 0.018

7.931 ± 0.033

5.336 ± 0.021

6.244 ± 0.023

8.5 ± 0.027

1.498 ± 0.01

2.074 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski