Sphingomonas sp. AP4-R1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4605 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5J7J9|A0A6M5J7J9_9SPHN Ribosomal RNA small subunit methyltransferase E OS=Sphingomonas sp. AP4-R1 OX=2735134 GN=HL653_01760 PE=3 SV=1
MM1 pKa = 6.97KK2 pKa = 9.85TIFAAALAALALGTSAGAAVTYY24 pKa = 10.19HH25 pKa = 5.0VTVDD29 pKa = 3.31TSSQIGKK36 pKa = 9.82AGYY39 pKa = 9.75IDD41 pKa = 4.04LQFGAGCLGGGCNPQLATALVSGFTTDD68 pKa = 3.1GTLQPFDD75 pKa = 4.78GNDD78 pKa = 3.62PNDD81 pKa = 3.83LAEE84 pKa = 4.65GTTGTLPTGVSFDD97 pKa = 3.7NQTFTPSDD105 pKa = 4.04FSQAIIFGSSLSFAVTLSGPAIEE128 pKa = 5.56SPLNDD133 pKa = 3.24GGNYY137 pKa = 9.96VGTAFLFDD145 pKa = 4.45FGNADD150 pKa = 3.09FSDD153 pKa = 4.99FYY155 pKa = 11.23FSQNDD160 pKa = 3.66GGPFGFLAGALHH172 pKa = 6.42IQQDD176 pKa = 3.79GSVLVVANPSSQDD189 pKa = 3.4YY190 pKa = 7.97TQSSGLSFTTITAASDD206 pKa = 3.8VPEE209 pKa = 4.35PASWAMMLAGFGLVGGTMRR228 pKa = 11.84RR229 pKa = 11.84RR230 pKa = 11.84KK231 pKa = 8.97LTVSFAA237 pKa = 3.78

Molecular weight: 24.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5JJ19|A0A6M5JJ19_9SPHN TonB-dependent receptor OS=Sphingomonas sp. AP4-R1 OX=2735134 GN=HL653_13560 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.61GFRR19 pKa = 11.84SRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84SKK41 pKa = 11.12LSAA44 pKa = 3.81

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4605

0

4605

1512193

29

3870

328.4

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.587 ± 0.058

0.714 ± 0.01

5.864 ± 0.029

5.039 ± 0.035

3.468 ± 0.026

9.069 ± 0.049

1.97 ± 0.019

5.113 ± 0.026

2.826 ± 0.031

9.882 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.02

2.462 ± 0.029

5.486 ± 0.031

3.022 ± 0.019

7.41 ± 0.044

5.406 ± 0.03

5.61 ± 0.044

7.134 ± 0.027

1.404 ± 0.016

2.231 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski