Sumatran orang-utan polyomavirus
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C9X3W9|C9X3W9_9POLY Minor capsid protein VP2 OS=Sumatran orang-utan polyomavirus OX=1604875 GN=VP3 PE=3 SV=1
MM1 pKa = 7.49 GGVISLIVDD10 pKa = 4.19 IIEE13 pKa = 4.01 LTAEE17 pKa = 3.97 VTAATGFTVEE27 pKa = 5.01 AIINGEE33 pKa = 3.69 ALAAVEE39 pKa = 4.4 AQLSSLATVEE49 pKa = 4.17 GLSGVEE55 pKa = 4.03 ALASLGITAEE65 pKa = 4.01 QYY67 pKa = 10.29 TFLTSVPGMLSNAVGLGVVFQTVSGASALVTAGIATSYY105 pKa = 10.37 AKK107 pKa = 10.13 EE108 pKa = 3.83 VSVVNRR114 pKa = 11.84 NMALIPWRR122 pKa = 11.84 DD123 pKa = 3.07 PDD125 pKa = 3.8 YY126 pKa = 11.55 YY127 pKa = 11.05 DD128 pKa = 3.18 ILFPGVQSFSYY139 pKa = 10.67 AIDD142 pKa = 4.39 VITNWPGSLFHH153 pKa = 7.48 SIGRR157 pKa = 11.84 YY158 pKa = 8.43 IWDD161 pKa = 3.58 SVTSEE166 pKa = 3.54 ARR168 pKa = 11.84 GQIGHH173 pKa = 7.76 ASNQVIVRR181 pKa = 11.84 GTYY184 pKa = 10.37 AFQDD188 pKa = 3.34 SLARR192 pKa = 11.84 ILEE195 pKa = 3.92 NARR198 pKa = 11.84 WVVQAGPTNVYY209 pKa = 10.7 NFLDD213 pKa = 3.38 SYY215 pKa = 11.2 YY216 pKa = 10.71 RR217 pKa = 11.84 EE218 pKa = 4.43 LPPINPAQARR228 pKa = 11.84 QMYY231 pKa = 9.76 RR232 pKa = 11.84 RR233 pKa = 11.84 IGQKK237 pKa = 10.04 YY238 pKa = 8.62 RR239 pKa = 11.84 MPRR242 pKa = 11.84 EE243 pKa = 3.57 AEE245 pKa = 3.61 IPDD248 pKa = 3.88 RR249 pKa = 11.84 YY250 pKa = 10.5 NLEE253 pKa = 4.08 SSEE256 pKa = 4.41 SEE258 pKa = 3.74 SGEE261 pKa = 3.86 TVEE264 pKa = 5.8 FYY266 pKa = 10.85 KK267 pKa = 10.99 HH268 pKa = 6.39 PGGANQRR275 pKa = 11.84 VTPDD279 pKa = 2.51 WMLPLILGLYY289 pKa = 10.53 GDD291 pKa = 4.96 ITPTWDD297 pKa = 3.25 KK298 pKa = 10.81 YY299 pKa = 6.76 VHH301 pKa = 5.8 EE302 pKa = 4.51 VEE304 pKa = 5.83 EE305 pKa = 4.21 EE306 pKa = 4.26 DD307 pKa = 4.0 EE308 pKa = 4.22 LQKK311 pKa = 10.83 KK312 pKa = 8.7 KK313 pKa = 10.46 RR314 pKa = 11.84 RR315 pKa = 11.84 RR316 pKa = 11.84 LL317 pKa = 3.37
Molecular weight: 34.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.803
IPC2_protein 4.876
IPC_protein 4.787
Toseland 4.673
ProMoST 4.889
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.571
Rodwell 4.647
Grimsley 4.584
Solomon 4.724
Lehninger 4.685
Nozaki 4.838
DTASelect 4.952
Thurlkill 4.66
EMBOSS 4.596
Sillero 4.914
Patrickios 3.757
IPC_peptide 4.736
IPC2_peptide 4.914
IPC2.peptide.svr19 4.867
Protein with the highest isoelectric point:
>tr|C9X3X2|C9X3X2_9POLY Small T antigen OS=Sumatran orang-utan polyomavirus OX=1604875 GN=small T PE=4 SV=1
MM1 pKa = 7.92 DD2 pKa = 6.35 KK3 pKa = 10.61 ILTKK7 pKa = 7.75 QQRR10 pKa = 11.84 KK11 pKa = 7.55 EE12 pKa = 4.17 LIVLLDD18 pKa = 4.45 IEE20 pKa = 4.49 ACDD23 pKa = 3.64 YY24 pKa = 11.64 GNYY27 pKa = 10.49 SIMKK31 pKa = 8.03 QQYY34 pKa = 10.42 KK35 pKa = 10.27 KK36 pKa = 9.56 MCLIYY41 pKa = 10.7 HH42 pKa = 6.92 PDD44 pKa = 3.33 KK45 pKa = 11.58 GGDD48 pKa = 3.62 GEE50 pKa = 5.38 KK51 pKa = 9.46 MRR53 pKa = 11.84 KK54 pKa = 9.29 LNSLWQAFNTEE65 pKa = 4.02 LLEE68 pKa = 4.44 IRR70 pKa = 11.84 DD71 pKa = 3.73 NRR73 pKa = 11.84 FDD75 pKa = 3.76 MEE77 pKa = 4.35 QVRR80 pKa = 11.84 QMDD83 pKa = 3.35 IDD85 pKa = 3.59 VWDD88 pKa = 4.81 DD89 pKa = 3.5 LLTVKK94 pKa = 10.32 EE95 pKa = 4.1 ALDD98 pKa = 4.01 DD99 pKa = 4.1 FEE101 pKa = 6.77 HH102 pKa = 7.31 IFVKK106 pKa = 10.23 VVPSCFNRR114 pKa = 11.84 VAVCKK119 pKa = 9.86 CKK121 pKa = 10.74 CIVCKK126 pKa = 10.44 LRR128 pKa = 11.84 QQHH131 pKa = 7.0 LIIKK135 pKa = 6.5 EE136 pKa = 4.04 HH137 pKa = 6.05 KK138 pKa = 9.99 RR139 pKa = 11.84 CVLWGEE145 pKa = 4.4 CFCHH149 pKa = 5.97 KK150 pKa = 10.46 CYY152 pKa = 11.0 VEE154 pKa = 4.12 WYY156 pKa = 8.85 GLSGTEE162 pKa = 4.68 QHH164 pKa = 6.79 LIWWKK169 pKa = 10.73 KK170 pKa = 9.92 IIQDD174 pKa = 3.6 TPFIFMRR181 pKa = 11.84 LNTQDD186 pKa = 3.34 TLKK189 pKa = 10.75 PSLIYY194 pKa = 10.71
Molecular weight: 23.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.808
IPC2_protein 7.0
IPC_protein 6.942
Toseland 6.634
ProMoST 7.629
Dawson 7.614
Bjellqvist 7.966
Wikipedia 7.527
Rodwell 7.629
Grimsley 6.678
Solomon 7.658
Lehninger 7.688
Nozaki 8.317
DTASelect 7.775
Thurlkill 7.834
EMBOSS 7.863
Sillero 8.17
Patrickios 4.075
IPC_peptide 7.658
IPC2_peptide 7.293
IPC2.peptide.svr19 7.191
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1828
194
735
365.6
41.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.853 ± 0.864
2.681 ± 0.831
5.635 ± 0.403
6.729 ± 0.345
4.431 ± 0.711
5.744 ± 0.732
1.641 ± 0.323
5.197 ± 0.814
6.783 ± 1.346
9.136 ± 0.668
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.681 ± 0.248
4.486 ± 0.333
5.47 ± 0.603
4.431 ± 0.289
4.869 ± 0.627
7.002 ± 0.715
5.525 ± 0.744
6.127 ± 0.876
1.477 ± 0.417
4.103 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here