Bat associated cyclovirus 13
Average proteome isoelectric point is 7.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3MCJ9|A0A0D3MCJ9_9CIRC ATP-dependent helicase Rep OS=Bat associated cyclovirus 13 OX=1987738 PE=3 SV=1
MM1 pKa = 7.52 ANSTVRR7 pKa = 11.84 RR8 pKa = 11.84 FCFTWNNYY16 pKa = 7.38 TEE18 pKa = 4.52 PDD20 pKa = 3.54 YY21 pKa = 11.08 EE22 pKa = 4.13 KK23 pKa = 10.56 CKK25 pKa = 10.21 TFIVEE30 pKa = 4.11 LCKK33 pKa = 10.93 YY34 pKa = 9.58 GIVGKK39 pKa = 10.06 EE40 pKa = 3.69 VAPNTGTNHH49 pKa = 6.0 LQGFCSLKK57 pKa = 10.23 KK58 pKa = 10.1 PMRR61 pKa = 11.84 FSTIKK66 pKa = 10.18 KK67 pKa = 9.28 RR68 pKa = 11.84 LDD70 pKa = 3.24 NRR72 pKa = 11.84 IHH74 pKa = 6.36 IEE76 pKa = 3.96 KK77 pKa = 10.88 ANGSDD82 pKa = 4.26 DD83 pKa = 5.43 DD84 pKa = 4.2 NEE86 pKa = 4.63 KK87 pKa = 10.58 YY88 pKa = 10.24 CSKK91 pKa = 11.02 AGDD94 pKa = 3.76 FFVSGDD100 pKa = 3.57 KK101 pKa = 10.82 DD102 pKa = 3.5 KK103 pKa = 11.61 QGARR107 pKa = 11.84 NDD109 pKa = 3.28 IQAVISAIQNGEE121 pKa = 3.79 VTTPKK126 pKa = 9.83 MVAAVYY132 pKa = 9.53 GSVYY136 pKa = 10.1 IKK138 pKa = 8.23 YY139 pKa = 9.82 HH140 pKa = 6.2 RR141 pKa = 11.84 GIEE144 pKa = 4.24 KK145 pKa = 10.35 YY146 pKa = 10.58 LSLLRR151 pKa = 11.84 PVPEE155 pKa = 4.29 RR156 pKa = 11.84 NFKK159 pKa = 10.07 TEE161 pKa = 3.68 LRR163 pKa = 11.84 IYY165 pKa = 9.27 WGPPGSGKK173 pKa = 8.76 SRR175 pKa = 11.84 RR176 pKa = 11.84 ALEE179 pKa = 4.1 EE180 pKa = 4.11 ANSLGSSVYY189 pKa = 10.24 YY190 pKa = 10.24 KK191 pKa = 10.61 PRR193 pKa = 11.84 GLWWDD198 pKa = 3.85 GYY200 pKa = 7.45 EE201 pKa = 4.15 QQDD204 pKa = 4.01 SVIIDD209 pKa = 4.35 DD210 pKa = 4.43 FYY212 pKa = 11.87 GWIKK216 pKa = 10.75 YY217 pKa = 10.28 DD218 pKa = 4.1 EE219 pKa = 4.44 LLKK222 pKa = 10.71 ICDD225 pKa = 3.53 RR226 pKa = 11.84 YY227 pKa = 9.77 PYY229 pKa = 9.73 KK230 pKa = 11.01 VQVKK234 pKa = 10.49 GSFQEE239 pKa = 4.35 FTSKK243 pKa = 11.12 YY244 pKa = 10.58 IFITSNTDD252 pKa = 2.29 IYY254 pKa = 11.28 SWYY257 pKa = 10.0 KK258 pKa = 9.5 FPGYY262 pKa = 7.88 EE263 pKa = 3.47 TAAIEE268 pKa = 4.12 RR269 pKa = 11.84 RR270 pKa = 11.84 AEE272 pKa = 3.45 IHH274 pKa = 6.59 EE275 pKa = 4.49 YY276 pKa = 10.32 IGGG279 pKa = 3.7
Molecular weight: 32.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.843
IPC2_protein 8.039
IPC_protein 7.922
Toseland 7.907
ProMoST 8.404
Dawson 8.653
Bjellqvist 8.799
Wikipedia 8.682
Rodwell 8.712
Grimsley 7.849
Solomon 8.77
Lehninger 8.756
Nozaki 8.858
DTASelect 8.609
Thurlkill 8.668
EMBOSS 8.829
Sillero 8.916
Patrickios 4.05
IPC_peptide 8.756
IPC2_peptide 7.6
IPC2.peptide.svr19 7.821
Protein with the highest isoelectric point:
>tr|A0A0D3MCJ9|A0A0D3MCJ9_9CIRC ATP-dependent helicase Rep OS=Bat associated cyclovirus 13 OX=1987738 PE=3 SV=1
MM1 pKa = 7.52 ANSTVRR7 pKa = 11.84 RR8 pKa = 11.84 FCFTWNNYY16 pKa = 7.38 TEE18 pKa = 4.52 PDD20 pKa = 3.54 YY21 pKa = 11.08 EE22 pKa = 4.13 KK23 pKa = 10.56 CKK25 pKa = 10.21 TFIVEE30 pKa = 4.11 LCKK33 pKa = 10.93 YY34 pKa = 9.58 GIVGKK39 pKa = 10.06 EE40 pKa = 3.69 VAPNTGTNHH49 pKa = 6.0 LQGFCSLKK57 pKa = 10.23 KK58 pKa = 10.1 PMRR61 pKa = 11.84 FSTIKK66 pKa = 10.18 KK67 pKa = 9.28 RR68 pKa = 11.84 LDD70 pKa = 3.24 NRR72 pKa = 11.84 IHH74 pKa = 6.36 IEE76 pKa = 3.96 KK77 pKa = 10.88 ANGSDD82 pKa = 4.26 DD83 pKa = 5.43 DD84 pKa = 4.2 NEE86 pKa = 4.63 KK87 pKa = 10.58 YY88 pKa = 10.24 CSKK91 pKa = 11.02 AGDD94 pKa = 3.76 FFVSGDD100 pKa = 3.57 KK101 pKa = 10.82 DD102 pKa = 3.5 KK103 pKa = 11.61 QGARR107 pKa = 11.84 NDD109 pKa = 3.28 IQAVISAIQNGEE121 pKa = 3.79 VTTPKK126 pKa = 9.83 MVAAVYY132 pKa = 9.53 GSVYY136 pKa = 10.1 IKK138 pKa = 8.23 YY139 pKa = 9.82 HH140 pKa = 6.2 RR141 pKa = 11.84 GIEE144 pKa = 4.24 KK145 pKa = 10.35 YY146 pKa = 10.58 LSLLRR151 pKa = 11.84 PVPEE155 pKa = 4.29 RR156 pKa = 11.84 NFKK159 pKa = 10.07 TEE161 pKa = 3.68 LRR163 pKa = 11.84 IYY165 pKa = 9.27 WGPPGSGKK173 pKa = 8.76 SRR175 pKa = 11.84 RR176 pKa = 11.84 ALEE179 pKa = 4.1 EE180 pKa = 4.11 ANSLGSSVYY189 pKa = 10.24 YY190 pKa = 10.24 KK191 pKa = 10.61 PRR193 pKa = 11.84 GLWWDD198 pKa = 3.85 GYY200 pKa = 7.45 EE201 pKa = 4.15 QQDD204 pKa = 4.01 SVIIDD209 pKa = 4.35 DD210 pKa = 4.43 FYY212 pKa = 11.87 GWIKK216 pKa = 10.75 YY217 pKa = 10.28 DD218 pKa = 4.1 EE219 pKa = 4.44 LLKK222 pKa = 10.71 ICDD225 pKa = 3.53 RR226 pKa = 11.84 YY227 pKa = 9.77 PYY229 pKa = 9.73 KK230 pKa = 11.01 VQVKK234 pKa = 10.49 GSFQEE239 pKa = 4.35 FTSKK243 pKa = 11.12 YY244 pKa = 10.58 IFITSNTDD252 pKa = 2.29 IYY254 pKa = 11.28 SWYY257 pKa = 10.0 KK258 pKa = 9.5 FPGYY262 pKa = 7.88 EE263 pKa = 3.47 TAAIEE268 pKa = 4.12 RR269 pKa = 11.84 RR270 pKa = 11.84 AEE272 pKa = 3.45 IHH274 pKa = 6.59 EE275 pKa = 4.49 YY276 pKa = 10.32 IGGG279 pKa = 3.7
Molecular weight: 32.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.843
IPC2_protein 8.039
IPC_protein 7.922
Toseland 7.907
ProMoST 8.404
Dawson 8.653
Bjellqvist 8.799
Wikipedia 8.682
Rodwell 8.712
Grimsley 7.849
Solomon 8.77
Lehninger 8.756
Nozaki 8.858
DTASelect 8.609
Thurlkill 8.668
EMBOSS 8.829
Sillero 8.916
Patrickios 4.05
IPC_peptide 8.756
IPC2_peptide 7.6
IPC2.peptide.svr19 7.821
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
279
279
279
279.0
32.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.018 ± 0.0
2.151 ± 0.0
5.735 ± 0.0
6.81 ± 0.0
4.659 ± 0.0
7.885 ± 0.0
1.434 ± 0.0
7.527 ± 0.0
8.961 ± 0.0
4.659 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.075 ± 0.0
4.659 ± 0.0
3.943 ± 0.0
2.867 ± 0.0
5.735 ± 0.0
6.81 ± 0.0
5.018 ± 0.0
5.376 ± 0.0
2.151 ± 0.0
7.527 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here