Clohesyomyces aquaticus
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15806 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y1Y6W5|A0A1Y1Y6W5_9PLEO Uncharacterized protein OS=Clohesyomyces aquaticus OX=1231657 GN=BCR34DRAFT_235437 PE=4 SV=1
MM1 pKa = 6.66 GTALASPASHH11 pKa = 7.19 LYY13 pKa = 8.79 SAKK16 pKa = 10.32 RR17 pKa = 11.84 QTATPDD23 pKa = 2.75 IAGAITQWNLDD34 pKa = 3.68 VQTVNTFLNHH44 pKa = 7.79 AIGQSQMDD52 pKa = 4.19 LLIAARR58 pKa = 11.84 NALNFASDD66 pKa = 3.86 EE67 pKa = 4.32 PNEE70 pKa = 3.91 LMILAMSQGLADD82 pKa = 3.83 NALAAIQDD90 pKa = 3.76 LMNVFGNVPTAIQSVIDD107 pKa = 4.34 NPDD110 pKa = 3.31 AGNVATQVGVINQVRR125 pKa = 11.84 CCNVLPDD132 pKa = 5.65 LNVLWTAAAADD143 pKa = 4.01 AGVVGQVNLVVPLPNACNDD162 pKa = 3.39 GSVVCPP168 pKa = 4.04
Molecular weight: 17.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.656
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.719
Grimsley 3.579
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.342
Thurlkill 3.745
EMBOSS 3.91
Sillero 4.024
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A1Y1ZIN8|A0A1Y1ZIN8_9PLEO RTA1 like protein-domain-containing protein OS=Clohesyomyces aquaticus OX=1231657 GN=BCR34DRAFT_540564 PE=4 SV=1
MM1 pKa = 7.1 FPSMGPLLAKK11 pKa = 10.0 FLRR14 pKa = 11.84 PPVLGLLLTHH24 pKa = 7.12 KK25 pKa = 10.18 LLPLSLPPTPRR36 pKa = 11.84 PRR38 pKa = 11.84 RR39 pKa = 11.84 NTRR42 pKa = 11.84 RR43 pKa = 11.84 QLHH46 pKa = 5.68 NPHH49 pKa = 6.15 VLSIQLQQITNLSPSPSSRR68 pKa = 11.84 STTIPRR74 pKa = 11.84 NSGVSSPAQLASSQTLSLARR94 pKa = 11.84 LPPTT98 pKa = 3.93
Molecular weight: 10.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.506
IPC2_protein 11.14
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.501
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.237
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.157
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15806
0
15806
6750781
49
12447
427.1
47.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.406 ± 0.018
1.392 ± 0.008
5.39 ± 0.014
6.015 ± 0.02
3.803 ± 0.013
6.978 ± 0.021
2.464 ± 0.009
4.953 ± 0.015
4.917 ± 0.019
8.889 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.2 ± 0.007
3.688 ± 0.011
6.234 ± 0.022
3.869 ± 0.014
6.151 ± 0.017
8.237 ± 0.02
6.019 ± 0.014
6.039 ± 0.013
1.562 ± 0.008
2.792 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here