Hubei narna-like virus 17
Average proteome isoelectric point is 9.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIF7|A0A1L3KIF7_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 17 OX=1922947 PE=4 SV=1
MM1 pKa = 7.34 VSPIGNLIVVRR12 pKa = 11.84 PRR14 pKa = 11.84 VYY16 pKa = 10.89 TMGMTAAILRR26 pKa = 11.84 RR27 pKa = 11.84 MVHH30 pKa = 6.61 RR31 pKa = 11.84 NLAPGSLMLSLSRR44 pKa = 11.84 SEE46 pKa = 3.68 RR47 pKa = 11.84 RR48 pKa = 11.84 LRR50 pKa = 11.84 QRR52 pKa = 11.84 SEE54 pKa = 4.93 VEE56 pKa = 4.14 QQWLSDD62 pKa = 3.53 FVRR65 pKa = 11.84 FADD68 pKa = 3.88 SAFALAIRR76 pKa = 11.84 THH78 pKa = 6.31 LRR80 pKa = 11.84 QLQGDD85 pKa = 4.05 DD86 pKa = 3.62 VLAHH90 pKa = 6.23 MKK92 pKa = 9.77 TMARR96 pKa = 11.84 LYY98 pKa = 11.1 AFLAKK103 pKa = 10.77 NEE105 pKa = 4.11 VKK107 pKa = 10.56 KK108 pKa = 10.62 IASWWKK114 pKa = 8.76 EE115 pKa = 3.47 NCFQLLDD122 pKa = 3.83 RR123 pKa = 11.84 SLQAGDD129 pKa = 3.74 EE130 pKa = 4.43 ANNATGDD137 pKa = 3.91 NEE139 pKa = 4.42 VNTSFTRR146 pKa = 11.84 SALAAASKK154 pKa = 10.75 RR155 pKa = 11.84 KK156 pKa = 8.94 MKK158 pKa = 10.37 RR159 pKa = 11.84 ARR161 pKa = 11.84 KK162 pKa = 9.29 DD163 pKa = 3.14 RR164 pKa = 11.84 QRR166 pKa = 11.84 VKK168 pKa = 10.95 KK169 pKa = 10.91 LMGTRR174 pKa = 11.84 SSRR177 pKa = 11.84 QVSSLFASLIRR188 pKa = 11.84 SSGLQSFRR196 pKa = 11.84 EE197 pKa = 5.07 GIALGLSARR206 pKa = 11.84 ILPAPILTEE215 pKa = 4.38 PIVEE219 pKa = 4.17 DD220 pKa = 3.37 EE221 pKa = 3.92 ARR223 pKa = 11.84 KK224 pKa = 9.7 LRR226 pKa = 11.84 EE227 pKa = 3.95 RR228 pKa = 11.84 LTGRR232 pKa = 11.84 PVVIPPEE239 pKa = 4.11 DD240 pKa = 3.48 AGIHH244 pKa = 4.41 QSIGEE249 pKa = 4.18 TMLRR253 pKa = 11.84 ISNRR257 pKa = 11.84 IKK259 pKa = 10.7 RR260 pKa = 11.84 FIKK263 pKa = 10.46 VEE265 pKa = 3.86 GRR267 pKa = 11.84 KK268 pKa = 9.17 IPLPTDD274 pKa = 3.38 SKK276 pKa = 10.88 IVSEE280 pKa = 4.36 ADD282 pKa = 2.98 THH284 pKa = 6.26 EE285 pKa = 4.43 VLTSRR290 pKa = 11.84 CIAHH294 pKa = 6.91 AAEE297 pKa = 4.52 SSNGRR302 pKa = 11.84 YY303 pKa = 6.96 EE304 pKa = 4.05 TFIGNVRR311 pKa = 11.84 TVEE314 pKa = 3.85 AKK316 pKa = 10.11 PRR318 pKa = 11.84 INKK321 pKa = 8.43 TLFLAEE327 pKa = 4.24 PGYY330 pKa = 10.01 KK331 pKa = 9.86 VRR333 pKa = 11.84 VASIPPCSSMVTAGRR348 pKa = 11.84 TVNQLLLRR356 pKa = 11.84 AISKK360 pKa = 9.9 AYY362 pKa = 8.69 PAQTSLGIGTPKK374 pKa = 10.41 EE375 pKa = 3.68 FLAEE379 pKa = 4.18 FKK381 pKa = 10.89 KK382 pKa = 10.62 CDD384 pKa = 3.52 RR385 pKa = 11.84 QCRR388 pKa = 11.84 WLYY391 pKa = 10.16 STDD394 pKa = 2.9 LTAATDD400 pKa = 4.78 HH401 pKa = 6.95 IDD403 pKa = 2.98 SSYY406 pKa = 11.83 AMSAVEE412 pKa = 4.76 DD413 pKa = 5.23 LITEE417 pKa = 4.24 MVNQGFTAAEE427 pKa = 4.22 SVFWDD432 pKa = 4.12 QLPEE436 pKa = 4.0 MQTMAVCHH444 pKa = 5.31 QNSRR448 pKa = 11.84 EE449 pKa = 3.96 PFMSFTMKK457 pKa = 10.44 KK458 pKa = 9.25 GVLMGKK464 pKa = 9.16 PLTWPILTILQLTALEE480 pKa = 4.32 HH481 pKa = 6.51 AGLLARR487 pKa = 11.84 SHH489 pKa = 6.51 IVGDD493 pKa = 3.79 DD494 pKa = 3.37 ALVYY498 pKa = 10.63 ASEE501 pKa = 4.46 EE502 pKa = 4.2 EE503 pKa = 4.1 YY504 pKa = 11.42 ADD506 pKa = 3.82 YY507 pKa = 11.46 VKK509 pKa = 9.82 TAQAMGLVFNPSKK522 pKa = 8.02 THH524 pKa = 5.14 RR525 pKa = 11.84 TRR527 pKa = 11.84 SYY529 pKa = 11.25 GILAQNVIKK538 pKa = 10.72 LVGVPTTRR546 pKa = 11.84 DD547 pKa = 3.28 VKK549 pKa = 11.1 DD550 pKa = 3.68 KK551 pKa = 10.8 IVRR554 pKa = 11.84 TTEE557 pKa = 3.55 NTLWAHH563 pKa = 6.11 IEE565 pKa = 3.7 EE566 pKa = 4.59 AARR569 pKa = 11.84 KK570 pKa = 8.92 QGLVHH575 pKa = 7.1 FGMDD579 pKa = 3.24 NVRR582 pKa = 11.84 SPRR585 pKa = 11.84 RR586 pKa = 11.84 YY587 pKa = 8.08 EE588 pKa = 3.9 SYY590 pKa = 11.12 AFRR593 pKa = 11.84 IEE595 pKa = 3.8 QQIPYY600 pKa = 9.84 VPEE603 pKa = 3.73 LLAARR608 pKa = 11.84 GQLRR612 pKa = 11.84 KK613 pKa = 10.24 DD614 pKa = 3.32 PYY616 pKa = 11.64 APFNLPSVRR625 pKa = 11.84 RR626 pKa = 11.84 LATLPRR632 pKa = 11.84 WIRR635 pKa = 11.84 DD636 pKa = 3.4 RR637 pKa = 11.84 VPNAFARR644 pKa = 11.84 ANPQATKK651 pKa = 10.74 VLLKK655 pKa = 10.45 RR656 pKa = 11.84 GVDD659 pKa = 3.01 ISYY662 pKa = 9.48 PVQFGGAAVMSVTTIKK678 pKa = 10.78 RR679 pKa = 11.84 MTKK682 pKa = 9.13 RR683 pKa = 11.84 HH684 pKa = 5.68 LKK686 pKa = 9.57 GAYY689 pKa = 9.63 ALGRR693 pKa = 11.84 YY694 pKa = 8.68 ILANPTSYY702 pKa = 10.72 TRR704 pKa = 11.84 MSRR707 pKa = 11.84 LMTTQATHH715 pKa = 6.01 KK716 pKa = 9.31 RR717 pKa = 11.84 VTVTPVAITVEE728 pKa = 4.43 KK729 pKa = 10.52 VLEE732 pKa = 5.09 DD733 pKa = 3.34 IPTVPKK739 pKa = 10.94 GKK741 pKa = 10.53 GCDD744 pKa = 2.83 IKK746 pKa = 8.62 TTRR749 pKa = 11.84 EE750 pKa = 3.8 AVRR753 pKa = 11.84 SITNDD758 pKa = 3.09 ALVLLEE764 pKa = 4.17 FAKK767 pKa = 10.74 ASQVKK772 pKa = 10.35 DD773 pKa = 3.49 RR774 pKa = 11.84 ITYY777 pKa = 9.58 RR778 pKa = 11.84 FEE780 pKa = 3.88 KK781 pKa = 7.71 TAHH784 pKa = 5.98 MAACSHH790 pKa = 6.94 PSMVLEE796 pKa = 4.15 SFANKK801 pKa = 8.42 ARR803 pKa = 11.84 KK804 pKa = 8.52 MKK806 pKa = 10.18 VPRR809 pKa = 11.84 PEE811 pKa = 3.64 TVYY814 pKa = 11.15 KK815 pKa = 10.24 LWKK818 pKa = 9.75 SLRR821 pKa = 11.84 EE822 pKa = 3.94 PNIDD826 pKa = 3.77 YY827 pKa = 10.94 DD828 pKa = 4.07 PEE830 pKa = 4.12 ALKK833 pKa = 10.8 DD834 pKa = 3.34 LWRR837 pKa = 11.84 LSNATGATIVPTVSTNKK854 pKa = 9.82 IEE856 pKa = 4.28 TLRR859 pKa = 11.84 LAKK862 pKa = 10.45 YY863 pKa = 10.04 SSLRR867 pKa = 11.84 NTSAQNLDD875 pKa = 3.5 TSTQDD880 pKa = 3.04 TKK882 pKa = 10.94 IIGNRR887 pKa = 11.84 EE888 pKa = 3.84 VSSSISLFGKK898 pKa = 9.94 IATILAGNLEE908 pKa = 4.47 LAQVQQITRR917 pKa = 11.84 KK918 pKa = 8.17 LTRR921 pKa = 11.84 LQRR924 pKa = 11.84 RR925 pKa = 11.84 AVRR928 pKa = 11.84 LGRR931 pKa = 11.84 RR932 pKa = 11.84 VRR934 pKa = 11.84 ASAPTKK940 pKa = 10.72 NSS942 pKa = 3.05
Molecular weight: 105.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 9.502
IPC_protein 9.765
Toseland 10.438
ProMoST 10.043
Dawson 10.555
Bjellqvist 10.204
Wikipedia 10.716
Rodwell 10.921
Grimsley 10.599
Solomon 10.599
Lehninger 10.584
Nozaki 10.423
DTASelect 10.204
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.482
Patrickios 10.584
IPC_peptide 10.613
IPC2_peptide 8.902
IPC2.peptide.svr19 8.659
Protein with the highest isoelectric point:
>tr|A0A1L3KIF7|A0A1L3KIF7_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 17 OX=1922947 PE=4 SV=1
MM1 pKa = 7.34 VSPIGNLIVVRR12 pKa = 11.84 PRR14 pKa = 11.84 VYY16 pKa = 10.89 TMGMTAAILRR26 pKa = 11.84 RR27 pKa = 11.84 MVHH30 pKa = 6.61 RR31 pKa = 11.84 NLAPGSLMLSLSRR44 pKa = 11.84 SEE46 pKa = 3.68 RR47 pKa = 11.84 RR48 pKa = 11.84 LRR50 pKa = 11.84 QRR52 pKa = 11.84 SEE54 pKa = 4.93 VEE56 pKa = 4.14 QQWLSDD62 pKa = 3.53 FVRR65 pKa = 11.84 FADD68 pKa = 3.88 SAFALAIRR76 pKa = 11.84 THH78 pKa = 6.31 LRR80 pKa = 11.84 QLQGDD85 pKa = 4.05 DD86 pKa = 3.62 VLAHH90 pKa = 6.23 MKK92 pKa = 9.77 TMARR96 pKa = 11.84 LYY98 pKa = 11.1 AFLAKK103 pKa = 10.77 NEE105 pKa = 4.11 VKK107 pKa = 10.56 KK108 pKa = 10.62 IASWWKK114 pKa = 8.76 EE115 pKa = 3.47 NCFQLLDD122 pKa = 3.83 RR123 pKa = 11.84 SLQAGDD129 pKa = 3.74 EE130 pKa = 4.43 ANNATGDD137 pKa = 3.91 NEE139 pKa = 4.42 VNTSFTRR146 pKa = 11.84 SALAAASKK154 pKa = 10.75 RR155 pKa = 11.84 KK156 pKa = 8.94 MKK158 pKa = 10.37 RR159 pKa = 11.84 ARR161 pKa = 11.84 KK162 pKa = 9.29 DD163 pKa = 3.14 RR164 pKa = 11.84 QRR166 pKa = 11.84 VKK168 pKa = 10.95 KK169 pKa = 10.91 LMGTRR174 pKa = 11.84 SSRR177 pKa = 11.84 QVSSLFASLIRR188 pKa = 11.84 SSGLQSFRR196 pKa = 11.84 EE197 pKa = 5.07 GIALGLSARR206 pKa = 11.84 ILPAPILTEE215 pKa = 4.38 PIVEE219 pKa = 4.17 DD220 pKa = 3.37 EE221 pKa = 3.92 ARR223 pKa = 11.84 KK224 pKa = 9.7 LRR226 pKa = 11.84 EE227 pKa = 3.95 RR228 pKa = 11.84 LTGRR232 pKa = 11.84 PVVIPPEE239 pKa = 4.11 DD240 pKa = 3.48 AGIHH244 pKa = 4.41 QSIGEE249 pKa = 4.18 TMLRR253 pKa = 11.84 ISNRR257 pKa = 11.84 IKK259 pKa = 10.7 RR260 pKa = 11.84 FIKK263 pKa = 10.46 VEE265 pKa = 3.86 GRR267 pKa = 11.84 KK268 pKa = 9.17 IPLPTDD274 pKa = 3.38 SKK276 pKa = 10.88 IVSEE280 pKa = 4.36 ADD282 pKa = 2.98 THH284 pKa = 6.26 EE285 pKa = 4.43 VLTSRR290 pKa = 11.84 CIAHH294 pKa = 6.91 AAEE297 pKa = 4.52 SSNGRR302 pKa = 11.84 YY303 pKa = 6.96 EE304 pKa = 4.05 TFIGNVRR311 pKa = 11.84 TVEE314 pKa = 3.85 AKK316 pKa = 10.11 PRR318 pKa = 11.84 INKK321 pKa = 8.43 TLFLAEE327 pKa = 4.24 PGYY330 pKa = 10.01 KK331 pKa = 9.86 VRR333 pKa = 11.84 VASIPPCSSMVTAGRR348 pKa = 11.84 TVNQLLLRR356 pKa = 11.84 AISKK360 pKa = 9.9 AYY362 pKa = 8.69 PAQTSLGIGTPKK374 pKa = 10.41 EE375 pKa = 3.68 FLAEE379 pKa = 4.18 FKK381 pKa = 10.89 KK382 pKa = 10.62 CDD384 pKa = 3.52 RR385 pKa = 11.84 QCRR388 pKa = 11.84 WLYY391 pKa = 10.16 STDD394 pKa = 2.9 LTAATDD400 pKa = 4.78 HH401 pKa = 6.95 IDD403 pKa = 2.98 SSYY406 pKa = 11.83 AMSAVEE412 pKa = 4.76 DD413 pKa = 5.23 LITEE417 pKa = 4.24 MVNQGFTAAEE427 pKa = 4.22 SVFWDD432 pKa = 4.12 QLPEE436 pKa = 4.0 MQTMAVCHH444 pKa = 5.31 QNSRR448 pKa = 11.84 EE449 pKa = 3.96 PFMSFTMKK457 pKa = 10.44 KK458 pKa = 9.25 GVLMGKK464 pKa = 9.16 PLTWPILTILQLTALEE480 pKa = 4.32 HH481 pKa = 6.51 AGLLARR487 pKa = 11.84 SHH489 pKa = 6.51 IVGDD493 pKa = 3.79 DD494 pKa = 3.37 ALVYY498 pKa = 10.63 ASEE501 pKa = 4.46 EE502 pKa = 4.2 EE503 pKa = 4.1 YY504 pKa = 11.42 ADD506 pKa = 3.82 YY507 pKa = 11.46 VKK509 pKa = 9.82 TAQAMGLVFNPSKK522 pKa = 8.02 THH524 pKa = 5.14 RR525 pKa = 11.84 TRR527 pKa = 11.84 SYY529 pKa = 11.25 GILAQNVIKK538 pKa = 10.72 LVGVPTTRR546 pKa = 11.84 DD547 pKa = 3.28 VKK549 pKa = 11.1 DD550 pKa = 3.68 KK551 pKa = 10.8 IVRR554 pKa = 11.84 TTEE557 pKa = 3.55 NTLWAHH563 pKa = 6.11 IEE565 pKa = 3.7 EE566 pKa = 4.59 AARR569 pKa = 11.84 KK570 pKa = 8.92 QGLVHH575 pKa = 7.1 FGMDD579 pKa = 3.24 NVRR582 pKa = 11.84 SPRR585 pKa = 11.84 RR586 pKa = 11.84 YY587 pKa = 8.08 EE588 pKa = 3.9 SYY590 pKa = 11.12 AFRR593 pKa = 11.84 IEE595 pKa = 3.8 QQIPYY600 pKa = 9.84 VPEE603 pKa = 3.73 LLAARR608 pKa = 11.84 GQLRR612 pKa = 11.84 KK613 pKa = 10.24 DD614 pKa = 3.32 PYY616 pKa = 11.64 APFNLPSVRR625 pKa = 11.84 RR626 pKa = 11.84 LATLPRR632 pKa = 11.84 WIRR635 pKa = 11.84 DD636 pKa = 3.4 RR637 pKa = 11.84 VPNAFARR644 pKa = 11.84 ANPQATKK651 pKa = 10.74 VLLKK655 pKa = 10.45 RR656 pKa = 11.84 GVDD659 pKa = 3.01 ISYY662 pKa = 9.48 PVQFGGAAVMSVTTIKK678 pKa = 10.78 RR679 pKa = 11.84 MTKK682 pKa = 9.13 RR683 pKa = 11.84 HH684 pKa = 5.68 LKK686 pKa = 9.57 GAYY689 pKa = 9.63 ALGRR693 pKa = 11.84 YY694 pKa = 8.68 ILANPTSYY702 pKa = 10.72 TRR704 pKa = 11.84 MSRR707 pKa = 11.84 LMTTQATHH715 pKa = 6.01 KK716 pKa = 9.31 RR717 pKa = 11.84 VTVTPVAITVEE728 pKa = 4.43 KK729 pKa = 10.52 VLEE732 pKa = 5.09 DD733 pKa = 3.34 IPTVPKK739 pKa = 10.94 GKK741 pKa = 10.53 GCDD744 pKa = 2.83 IKK746 pKa = 8.62 TTRR749 pKa = 11.84 EE750 pKa = 3.8 AVRR753 pKa = 11.84 SITNDD758 pKa = 3.09 ALVLLEE764 pKa = 4.17 FAKK767 pKa = 10.74 ASQVKK772 pKa = 10.35 DD773 pKa = 3.49 RR774 pKa = 11.84 ITYY777 pKa = 9.58 RR778 pKa = 11.84 FEE780 pKa = 3.88 KK781 pKa = 7.71 TAHH784 pKa = 5.98 MAACSHH790 pKa = 6.94 PSMVLEE796 pKa = 4.15 SFANKK801 pKa = 8.42 ARR803 pKa = 11.84 KK804 pKa = 8.52 MKK806 pKa = 10.18 VPRR809 pKa = 11.84 PEE811 pKa = 3.64 TVYY814 pKa = 11.15 KK815 pKa = 10.24 LWKK818 pKa = 9.75 SLRR821 pKa = 11.84 EE822 pKa = 3.94 PNIDD826 pKa = 3.77 YY827 pKa = 10.94 DD828 pKa = 4.07 PEE830 pKa = 4.12 ALKK833 pKa = 10.8 DD834 pKa = 3.34 LWRR837 pKa = 11.84 LSNATGATIVPTVSTNKK854 pKa = 9.82 IEE856 pKa = 4.28 TLRR859 pKa = 11.84 LAKK862 pKa = 10.45 YY863 pKa = 10.04 SSLRR867 pKa = 11.84 NTSAQNLDD875 pKa = 3.5 TSTQDD880 pKa = 3.04 TKK882 pKa = 10.94 IIGNRR887 pKa = 11.84 EE888 pKa = 3.84 VSSSISLFGKK898 pKa = 9.94 IATILAGNLEE908 pKa = 4.47 LAQVQQITRR917 pKa = 11.84 KK918 pKa = 8.17 LTRR921 pKa = 11.84 LQRR924 pKa = 11.84 RR925 pKa = 11.84 AVRR928 pKa = 11.84 LGRR931 pKa = 11.84 RR932 pKa = 11.84 VRR934 pKa = 11.84 ASAPTKK940 pKa = 10.72 NSS942 pKa = 3.05
Molecular weight: 105.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 9.502
IPC_protein 9.765
Toseland 10.438
ProMoST 10.043
Dawson 10.555
Bjellqvist 10.204
Wikipedia 10.716
Rodwell 10.921
Grimsley 10.599
Solomon 10.599
Lehninger 10.584
Nozaki 10.423
DTASelect 10.204
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.482
Patrickios 10.584
IPC_peptide 10.613
IPC2_peptide 8.902
IPC2.peptide.svr19 8.659
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
942
942
942
942.0
105.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.873 ± 0.0
0.849 ± 0.0
3.822 ± 0.0
5.308 ± 0.0
2.866 ± 0.0
4.565 ± 0.0
1.805 ± 0.0
5.626 ± 0.0
6.157 ± 0.0
9.448 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.866 ± 0.0
3.503 ± 0.0
4.671 ± 0.0
3.715 ± 0.0
9.342 ± 0.0
7.431 ± 0.0
7.643 ± 0.0
7.006 ± 0.0
1.062 ± 0.0
2.442 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here