Thrips-associated genomovirus 3
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8YT72|A0A1P8YT72_9VIRU RepA OS=Thrips-associated genomovirus 3 OX=1941237 PE=3 SV=1
MM1 pKa = 7.97 PFRR4 pKa = 11.84 FAAKK8 pKa = 10.01 YY9 pKa = 10.66 GLLTYY14 pKa = 7.2 PQCGDD19 pKa = 3.72 LDD21 pKa = 3.7 PWSVSDD27 pKa = 3.44 HH28 pKa = 6.45 LGRR31 pKa = 11.84 LGAEE35 pKa = 4.22 CIIGRR40 pKa = 11.84 EE41 pKa = 4.15 DD42 pKa = 3.37 HH43 pKa = 7.1 SDD45 pKa = 3.54 GGVHH49 pKa = 6.1 LHH51 pKa = 6.35 AFFMFEE57 pKa = 3.8 RR58 pKa = 11.84 RR59 pKa = 11.84 FEE61 pKa = 4.22 SRR63 pKa = 11.84 DD64 pKa = 3.13 VRR66 pKa = 11.84 VFDD69 pKa = 4.26 VEE71 pKa = 4.3 GRR73 pKa = 11.84 HH74 pKa = 5.7 PNVVRR79 pKa = 11.84 GYY81 pKa = 7.64 STPSKK86 pKa = 10.33 GYY88 pKa = 10.77 AYY90 pKa = 8.25 ATKK93 pKa = 10.63 DD94 pKa = 3.16 GDD96 pKa = 4.0 VVAGGLEE103 pKa = 4.13 CPIDD107 pKa = 3.88 GASVSEE113 pKa = 5.0 ASSKK117 pKa = 7.87 WARR120 pKa = 11.84 AILAEE125 pKa = 4.02 SRR127 pKa = 11.84 EE128 pKa = 4.04 EE129 pKa = 3.76 FFAIVAEE136 pKa = 4.23 LDD138 pKa = 3.53 PRR140 pKa = 11.84 ALCCSFGSLRR150 pKa = 11.84 AYY152 pKa = 10.47 ADD154 pKa = 2.98 WKK156 pKa = 9.94 YY157 pKa = 11.07 RR158 pKa = 11.84 PVLDD162 pKa = 4.7 QYY164 pKa = 8.68 VTPAGISFCTDD175 pKa = 3.18 EE176 pKa = 5.02 VPQLHH181 pKa = 7.17 DD182 pKa = 3.09 WVQRR186 pKa = 11.84 NLAGSVVGEE195 pKa = 4.12
Molecular weight: 21.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.025
IPC2_protein 5.13
IPC_protein 5.067
Toseland 5.067
ProMoST 5.13
Dawson 5.105
Bjellqvist 5.245
Wikipedia 5.003
Rodwell 5.016
Grimsley 5.003
Solomon 5.105
Lehninger 5.067
Nozaki 5.245
DTASelect 5.41
Thurlkill 5.092
EMBOSS 5.08
Sillero 5.308
Patrickios 3.706
IPC_peptide 5.105
IPC2_peptide 5.308
IPC2.peptide.svr19 5.217
Protein with the highest isoelectric point:
>tr|A0A1P8YT72|A0A1P8YT72_9VIRU RepA OS=Thrips-associated genomovirus 3 OX=1941237 PE=3 SV=1
MM1 pKa = 7.0 YY2 pKa = 10.13 RR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 PSKK8 pKa = 10.19 RR9 pKa = 11.84 RR10 pKa = 11.84 SGGSRR15 pKa = 11.84 RR16 pKa = 11.84 TPRR19 pKa = 11.84 SSRR22 pKa = 11.84 RR23 pKa = 11.84 SYY25 pKa = 11.22 AKK27 pKa = 8.42 KK28 pKa = 8.0 TSYY31 pKa = 10.58 RR32 pKa = 11.84 RR33 pKa = 11.84 TRR35 pKa = 11.84 TVRR38 pKa = 11.84 KK39 pKa = 7.28 LTSRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 LLNITSQKK53 pKa = 10.32 KK54 pKa = 8.32 RR55 pKa = 11.84 DD56 pKa = 3.45 NMMPVTTDD64 pKa = 2.6 WTGQTPIPGPAVLNGAGRR82 pKa = 11.84 STILWCATQRR92 pKa = 11.84 DD93 pKa = 4.41 RR94 pKa = 11.84 ASTPYY99 pKa = 10.06 IDD101 pKa = 3.8 NSEE104 pKa = 4.12 AARR107 pKa = 11.84 TSKK110 pKa = 10.79 SIFLRR115 pKa = 11.84 GVRR118 pKa = 11.84 EE119 pKa = 3.9 FASIRR124 pKa = 11.84 TNSPDD129 pKa = 2.51 AWRR132 pKa = 11.84 WRR134 pKa = 11.84 RR135 pKa = 11.84 IIFSVKK141 pKa = 10.3 GIFVYY146 pKa = 10.74 LPLEE150 pKa = 3.84 SRR152 pKa = 11.84 FAVEE156 pKa = 4.2 TSNQGWSRR164 pKa = 11.84 AIPDD168 pKa = 3.64 YY169 pKa = 11.02 SGSASTTARR178 pKa = 11.84 NALEE182 pKa = 3.81 ALIFAGEE189 pKa = 4.07 AGKK192 pKa = 10.27 DD193 pKa = 3.12 WASVFTAKK201 pKa = 9.94 VDD203 pKa = 3.44 HH204 pKa = 6.81 SRR206 pKa = 11.84 ITPLFDD212 pKa = 2.81 KK213 pKa = 10.84 TRR215 pKa = 11.84 ILRR218 pKa = 11.84 SGNQQGSYY226 pKa = 10.28 FKK228 pKa = 11.06 NKK230 pKa = 9.33 LWLPVNKK237 pKa = 9.09 TLLYY241 pKa = 10.68 ADD243 pKa = 4.41 EE244 pKa = 4.51 EE245 pKa = 4.79 SGNNVQDD252 pKa = 3.95 GSLSAISRR260 pKa = 11.84 QGVGDD265 pKa = 3.53 IFIFDD270 pKa = 5.04 LFDD273 pKa = 5.17 CSTQNSASSLTFEE286 pKa = 5.37 PEE288 pKa = 3.28 ATMYY292 pKa = 8.49 WHH294 pKa = 6.85 EE295 pKa = 4.17 RR296 pKa = 3.48
Molecular weight: 33.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.853
IPC_protein 10.877
Toseland 10.935
ProMoST 11.008
Dawson 11.008
Bjellqvist 10.804
Wikipedia 11.301
Rodwell 11.082
Grimsley 11.052
Solomon 11.228
Lehninger 11.184
Nozaki 10.921
DTASelect 10.804
Thurlkill 10.935
EMBOSS 11.359
Sillero 10.95
Patrickios 10.774
IPC_peptide 11.242
IPC2_peptide 9.94
IPC2.peptide.svr19 8.654
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
809
195
318
269.7
30.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.776 ± 0.443
2.101 ± 0.64
6.799 ± 0.984
4.944 ± 0.581
5.315 ± 0.45
8.158 ± 0.965
2.225 ± 0.714
4.326 ± 0.484
3.832 ± 0.426
7.417 ± 0.609
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.236 ± 0.063
2.472 ± 0.854
4.574 ± 0.161
2.596 ± 0.202
8.9 ± 1.58
8.776 ± 1.483
5.068 ± 1.505
6.18 ± 0.958
2.472 ± 0.126
3.832 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here