Gigaspora margarita giardia-like virus 1
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L0VZI1|A0A2L0VZI1_9VIRU RNA-directed RNA polymerase OS=Gigaspora margarita giardia-like virus 1 OX=2082658 PE=3 SV=1
MM1 pKa = 7.3 SVDD4 pKa = 3.24 EE5 pKa = 4.71 MGLHH9 pKa = 7.09 LSYY12 pKa = 10.67 IKK14 pKa = 10.5 CKK16 pKa = 10.69 YY17 pKa = 9.94 DD18 pKa = 3.67 DD19 pKa = 4.33 NEE21 pKa = 4.19 MKK23 pKa = 10.37 ISNLRR28 pKa = 11.84 NLEE31 pKa = 4.02 YY32 pKa = 10.81 GKK34 pKa = 10.45 EE35 pKa = 3.81 FLMYY39 pKa = 9.53 PQEE42 pKa = 4.03 ISNMLKK48 pKa = 10.39 LHH50 pKa = 6.56 DD51 pKa = 4.32 KK52 pKa = 10.93 EE53 pKa = 5.76 FIANHH58 pKa = 6.34 KK59 pKa = 10.33 IKK61 pKa = 9.76 GTSLDD66 pKa = 3.46 YY67 pKa = 11.53 DD68 pKa = 3.6 GFDD71 pKa = 3.41 GVNIYY76 pKa = 10.29 IEE78 pKa = 5.57 DD79 pKa = 3.91 IIEE82 pKa = 4.43 DD83 pKa = 4.25 EE84 pKa = 4.07 NCILYY89 pKa = 9.58 KK90 pKa = 10.28 RR91 pKa = 11.84 YY92 pKa = 9.21 MEE94 pKa = 4.28 GLSNIFCLKK103 pKa = 10.37 DD104 pKa = 3.1 SRR106 pKa = 11.84 GRR108 pKa = 11.84 YY109 pKa = 8.75 NFSDD113 pKa = 3.38 YY114 pKa = 11.28 DD115 pKa = 3.98 YY116 pKa = 10.83 IIFLMAKK123 pKa = 9.57 GYY125 pKa = 10.58 GKK127 pKa = 8.62 STFCKK132 pKa = 10.37 DD133 pKa = 3.34 NKK135 pKa = 10.35 FCLDD139 pKa = 3.76 LDD141 pKa = 4.49 IILEE145 pKa = 4.16 KK146 pKa = 10.62 EE147 pKa = 4.2 QAQGRR152 pKa = 11.84 YY153 pKa = 6.71 TLPDD157 pKa = 3.97 DD158 pKa = 5.51 LEE160 pKa = 4.41 EE161 pKa = 4.8 FDD163 pKa = 3.1 WATYY167 pKa = 8.8 NKK169 pKa = 10.02 KK170 pKa = 9.94 IATLYY175 pKa = 10.01 SSQKK179 pKa = 6.83 ITEE182 pKa = 4.91 RR183 pKa = 11.84 ILFSNNMTYY192 pKa = 10.16 IDD194 pKa = 4.58 AIEE197 pKa = 4.34 GKK199 pKa = 9.77 KK200 pKa = 10.41 CVIKK204 pKa = 10.6 IIGPFNPYY212 pKa = 10.29 NSDD215 pKa = 3.93 RR216 pKa = 11.84 ISKK219 pKa = 9.75 DD220 pKa = 3.08 QYY222 pKa = 11.47 LLASMNYY229 pKa = 9.58 KK230 pKa = 9.86 HH231 pKa = 6.25 VQEE234 pKa = 4.54 SDD236 pKa = 3.47 CTLVADD242 pKa = 4.96 FTLNPEE248 pKa = 4.4 NIEE251 pKa = 4.31 DD252 pKa = 4.32 LLSGIHH258 pKa = 6.73 IEE260 pKa = 4.07 RR261 pKa = 11.84 NYY263 pKa = 9.98 KK264 pKa = 9.59 KK265 pKa = 10.58 EE266 pKa = 3.69 VMRR269 pKa = 11.84 VCKK272 pKa = 10.49 LINEE276 pKa = 4.43 KK277 pKa = 10.54 KK278 pKa = 10.38 KK279 pKa = 10.7 NLDD282 pKa = 2.66 KK283 pKa = 10.9 DD284 pKa = 4.11 YY285 pKa = 11.44 YY286 pKa = 9.87 FRR288 pKa = 11.84 SYY290 pKa = 11.48 NKK292 pKa = 9.28 FVRR295 pKa = 11.84 RR296 pKa = 11.84 RR297 pKa = 11.84 LMCKK301 pKa = 10.2 RR302 pKa = 11.84 ILYY305 pKa = 10.23 DD306 pKa = 4.21 DD307 pKa = 6.32 LIPFYY312 pKa = 10.89 DD313 pKa = 3.6 EE314 pKa = 4.95 SFSLMSDD321 pKa = 3.02 ASISFKK327 pKa = 11.15 NIFEE331 pKa = 4.34 SKK333 pKa = 10.2 YY334 pKa = 9.4 YY335 pKa = 9.92 PVLQGLRR342 pKa = 11.84 TAQQYY347 pKa = 10.64 EE348 pKa = 4.66 DD349 pKa = 3.97 YY350 pKa = 10.41 LALIGSMNKK359 pKa = 9.89 RR360 pKa = 11.84 YY361 pKa = 9.54 MIDD364 pKa = 3.08 GDD366 pKa = 3.56 NWIEE370 pKa = 3.95 FCDD373 pKa = 4.73 LGVCSGNYY381 pKa = 8.11 TSYY384 pKa = 11.47 DD385 pKa = 3.38 KK386 pKa = 11.07 EE387 pKa = 4.1 KK388 pKa = 10.87 CLEE391 pKa = 3.97 KK392 pKa = 10.66 LKK394 pKa = 11.04 YY395 pKa = 7.16 WTLEE399 pKa = 4.12 YY400 pKa = 10.51 KK401 pKa = 8.69 EE402 pKa = 4.33 HH403 pKa = 7.03 CIDD406 pKa = 3.35 GSEE409 pKa = 4.0 PHH411 pKa = 6.3 FLALFSEE418 pKa = 5.07 GVNEE422 pKa = 4.26 LFNNLEE428 pKa = 4.28 VIPRR432 pKa = 11.84 ADD434 pKa = 3.38 DD435 pKa = 3.13 QAYY438 pKa = 7.4 TFRR441 pKa = 11.84 EE442 pKa = 4.25 YY443 pKa = 11.47 LHH445 pKa = 6.78 LPKK448 pKa = 10.22 FWAVSGSTSIKK459 pKa = 10.26 RR460 pKa = 11.84 KK461 pKa = 9.56 IKK463 pKa = 10.46 VKK465 pKa = 7.55 TTNGEE470 pKa = 4.14 MKK472 pKa = 10.36 VKK474 pKa = 7.78 QTKK477 pKa = 8.09 WVNAIFDD484 pKa = 4.08 DD485 pKa = 4.58 HH486 pKa = 7.6 DD487 pKa = 3.68 EE488 pKa = 4.09 QYY490 pKa = 10.66 TRR492 pKa = 11.84 IMEE495 pKa = 4.31 KK496 pKa = 10.49 KK497 pKa = 8.55 KK498 pKa = 8.5 TADD501 pKa = 3.04 YY502 pKa = 11.47 KK503 pKa = 10.89 MIVKK507 pKa = 9.3 MGKK510 pKa = 8.03 GKK512 pKa = 8.32 TRR514 pKa = 11.84 IVVSGDD520 pKa = 2.91 LDD522 pKa = 3.96 LYY524 pKa = 10.86 AICDD528 pKa = 3.88 YY529 pKa = 11.08 IDD531 pKa = 3.46 YY532 pKa = 10.6 CVEE535 pKa = 4.05 RR536 pKa = 11.84 SIKK539 pKa = 10.11 SCSISMLYY547 pKa = 10.6 LDD549 pKa = 5.94 DD550 pKa = 4.88 DD551 pKa = 4.02 QKK553 pKa = 11.92 LDD555 pKa = 3.44 FWMKK559 pKa = 10.25 FCKK562 pKa = 10.3 CDD564 pKa = 3.34 DD565 pKa = 3.89 SYY567 pKa = 11.96 FYY569 pKa = 10.71 MPLDD573 pKa = 3.44 QSDD576 pKa = 3.71 YY577 pKa = 11.36 DD578 pKa = 3.55 QMVNKK583 pKa = 10.3 KK584 pKa = 8.25 MVNIVLEE591 pKa = 4.03 RR592 pKa = 11.84 FLNFFLLHH600 pKa = 6.52 AKK602 pKa = 10.52 DD603 pKa = 3.6 PVEE606 pKa = 3.99 ARR608 pKa = 11.84 SIFDD612 pKa = 3.38 QVWRR616 pKa = 11.84 CFEE619 pKa = 3.93 YY620 pKa = 11.03 GFIEE624 pKa = 4.47 IEE626 pKa = 4.08 DD627 pKa = 3.85 RR628 pKa = 11.84 KK629 pKa = 10.59 EE630 pKa = 4.08 LILNGVMSGYY640 pKa = 10.54 KK641 pKa = 7.38 WTAFFDD647 pKa = 4.03 TVINASEE654 pKa = 3.96 LHH656 pKa = 6.06 VIRR659 pKa = 11.84 TFLEE663 pKa = 4.09 KK664 pKa = 10.53 HH665 pKa = 5.7 SLACIRR671 pKa = 11.84 DD672 pKa = 3.59 VNFQGDD678 pKa = 4.6 DD679 pKa = 3.45 VCLMLDD685 pKa = 3.4 QDD687 pKa = 4.2 VATCYY692 pKa = 10.91 YY693 pKa = 10.28 IFWLYY698 pKa = 10.85 KK699 pKa = 9.81 KK700 pKa = 10.54 LGFLIHH706 pKa = 6.7 ANKK709 pKa = 9.85 FFLSNEE715 pKa = 3.7 RR716 pKa = 11.84 NEE718 pKa = 4.3 YY719 pKa = 9.08 LRR721 pKa = 11.84 KK722 pKa = 10.03 VSFKK726 pKa = 11.29 GEE728 pKa = 3.56 IRR730 pKa = 11.84 GYY732 pKa = 8.49 PARR735 pKa = 11.84 NVISLLQPDD744 pKa = 4.66 LMSIEE749 pKa = 4.94 SDD751 pKa = 3.51 DD752 pKa = 3.47 QRR754 pKa = 11.84 EE755 pKa = 4.1 RR756 pKa = 11.84 LTVYY760 pKa = 10.98 LNGYY764 pKa = 7.79 YY765 pKa = 10.48 NFRR768 pKa = 11.84 RR769 pKa = 11.84 RR770 pKa = 11.84 MRR772 pKa = 11.84 SPISLDD778 pKa = 3.41 DD779 pKa = 4.36 KK780 pKa = 11.03 IFYY783 pKa = 10.26 PEE785 pKa = 3.87 ADD787 pKa = 3.66 YY788 pKa = 11.15 YY789 pKa = 10.57 IPNASKK795 pKa = 10.71 LIHH798 pKa = 5.94 VPKK801 pKa = 10.52 SLGGFGFWPLVRR813 pKa = 11.84 STKK816 pKa = 9.96 YY817 pKa = 10.17 RR818 pKa = 11.84 FYY820 pKa = 11.27 KK821 pKa = 8.82 KK822 pKa = 10.13 QSYY825 pKa = 8.62 LQSVEE830 pKa = 3.95 QDD832 pKa = 3.36 DD833 pKa = 4.56 NLMKK837 pKa = 10.88 GIRR840 pKa = 11.84 EE841 pKa = 3.99 DD842 pKa = 3.49 PLYY845 pKa = 9.73 NTSYY849 pKa = 10.92 DD850 pKa = 3.51 GKK852 pKa = 10.57 KK853 pKa = 10.19 LFSILSPQAGRR864 pKa = 11.84 HH865 pKa = 4.82 RR866 pKa = 11.84 NNIRR870 pKa = 11.84 FGIRR874 pKa = 11.84 EE875 pKa = 4.29 LDD877 pKa = 3.41 SYY879 pKa = 10.46 ITLKK883 pKa = 11.06 SMNLYY888 pKa = 10.49 SDD890 pKa = 4.67 RR891 pKa = 11.84 LDD893 pKa = 3.81 TLPSDD898 pKa = 3.04 ISLYY902 pKa = 9.47 SFRR905 pKa = 11.84 NNSEE909 pKa = 3.96 NNSLRR914 pKa = 11.84 NSIFFDD920 pKa = 3.47 SYY922 pKa = 11.03 IRR924 pKa = 11.84 SLDD927 pKa = 3.27 ISDD930 pKa = 4.36 LEE932 pKa = 4.54 KK933 pKa = 10.7 IFNFSFNINNASLSVVKK950 pKa = 10.72 AIVNNEE956 pKa = 3.92 LFSAPKK962 pKa = 8.27 MFCYY966 pKa = 10.37 SEE968 pKa = 4.5 SSCSIVFNRR977 pKa = 11.84 LIKK980 pKa = 10.7 YY981 pKa = 10.5 NMLFFMKK988 pKa = 10.29 DD989 pKa = 2.74 NYY991 pKa = 9.82 LTLDD995 pKa = 3.26 KK996 pKa = 10.68 LKK998 pKa = 10.73 RR999 pKa = 11.84 IAFTMEE1005 pKa = 3.82 LKK1007 pKa = 10.27 FDD1009 pKa = 4.55 KK1010 pKa = 10.72 IMEE1013 pKa = 4.31 KK1014 pKa = 10.62 LYY1016 pKa = 10.82 DD1017 pKa = 4.01 CKK1019 pKa = 10.98 DD1020 pKa = 3.45 YY1021 pKa = 11.69 SS1022 pKa = 3.44
Molecular weight: 120.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.782
IPC2_protein 5.957
IPC_protein 5.995
Toseland 6.16
ProMoST 6.338
Dawson 6.275
Bjellqvist 6.3
Wikipedia 6.275
Rodwell 6.249
Grimsley 6.224
Solomon 6.275
Lehninger 6.262
Nozaki 6.532
DTASelect 6.707
Thurlkill 6.693
EMBOSS 6.664
Sillero 6.634
Patrickios 3.846
IPC_peptide 6.287
IPC2_peptide 6.478
IPC2.peptide.svr19 6.604
Protein with the highest isoelectric point:
>tr|A0A2L0VZI1|A0A2L0VZI1_9VIRU RNA-directed RNA polymerase OS=Gigaspora margarita giardia-like virus 1 OX=2082658 PE=3 SV=1
MM1 pKa = 7.65 KK2 pKa = 9.22 FTTTGEE8 pKa = 4.07 NAVMGVVPPDD18 pKa = 3.87 APDD21 pKa = 3.56 TTSQRR26 pKa = 11.84 YY27 pKa = 7.71 NATVHH32 pKa = 6.87 SNIEE36 pKa = 4.08 DD37 pKa = 3.53 VNGNRR42 pKa = 11.84 VINDD46 pKa = 3.65 TLSGTFHH53 pKa = 7.57 AYY55 pKa = 10.41 NYY57 pKa = 7.37 LTNPLGQRR65 pKa = 11.84 KK66 pKa = 9.09 RR67 pKa = 11.84 SVLTGDD73 pKa = 4.08 EE74 pKa = 4.21 NFMTYY79 pKa = 8.8 DD80 pKa = 3.44 TNIPQAGIYY89 pKa = 9.19 EE90 pKa = 4.32 RR91 pKa = 11.84 LALMIGTHH99 pKa = 5.26 QLVNKK104 pKa = 9.77 VNIEE108 pKa = 3.9 KK109 pKa = 10.65 YY110 pKa = 10.01 IEE112 pKa = 4.39 SNHH115 pKa = 5.75 FNHH118 pKa = 6.01 VVIGKK123 pKa = 9.33 HH124 pKa = 5.08 MASCLGKK131 pKa = 9.83 MGDD134 pKa = 3.99 VVLQQYY140 pKa = 8.59 DD141 pKa = 3.13 IPMCYY146 pKa = 10.03 LLEE149 pKa = 5.28 PIHH152 pKa = 6.04 GTQNHH157 pKa = 5.71 YY158 pKa = 11.33 KK159 pKa = 8.44 NTLLQRR165 pKa = 11.84 YY166 pKa = 6.92 YY167 pKa = 11.02 NRR169 pKa = 11.84 EE170 pKa = 3.86 HH171 pKa = 6.59 PFSRR175 pKa = 11.84 EE176 pKa = 3.68 MVSVYY181 pKa = 11.48 DD182 pKa = 3.97 MMSFDD187 pKa = 4.89 YY188 pKa = 11.03 LNKK191 pKa = 10.46 LKK193 pKa = 10.07 WDD195 pKa = 3.35 EE196 pKa = 4.21 RR197 pKa = 11.84 LWFSGQYY204 pKa = 10.63 SNPQGSGLANYY215 pKa = 10.28 LSDD218 pKa = 3.59 VYY220 pKa = 10.58 RR221 pKa = 11.84 YY222 pKa = 9.94 SKK224 pKa = 8.32 WHH226 pKa = 6.69 RR227 pKa = 11.84 EE228 pKa = 3.87 YY229 pKa = 10.62 STIGYY234 pKa = 7.54 DD235 pKa = 3.8 TNDD238 pKa = 3.49 HH239 pKa = 6.19 VSDD242 pKa = 5.11 LSFIDD247 pKa = 3.75 INWEE251 pKa = 3.9 KK252 pKa = 10.5 HH253 pKa = 4.95 VKK255 pKa = 9.56 DD256 pKa = 3.41 QIYY259 pKa = 10.4 YY260 pKa = 7.76 RR261 pKa = 11.84 TPDD264 pKa = 3.7 DD265 pKa = 3.23 QHH267 pKa = 7.38 VIRR270 pKa = 11.84 WVKK273 pKa = 9.51 KK274 pKa = 7.76 WVYY277 pKa = 9.84 EE278 pKa = 3.99 LNEE281 pKa = 4.48 LEE283 pKa = 4.26 SLDD286 pKa = 3.37 WTRR289 pKa = 11.84 LYY291 pKa = 11.2 GVDD294 pKa = 3.58 NNVDD298 pKa = 3.54 YY299 pKa = 11.62 VFGHH303 pKa = 6.02 NNRR306 pKa = 11.84 CIKK309 pKa = 10.06 VVNFIGKK316 pKa = 9.13 EE317 pKa = 3.92 NQSYY321 pKa = 11.07 NSFFKK326 pKa = 10.91 FMTEE330 pKa = 4.23 CNLHH334 pKa = 6.87 FYY336 pKa = 7.88 TKK338 pKa = 9.79 PVSIKK343 pKa = 10.63 SYY345 pKa = 10.67 DD346 pKa = 3.34 PRR348 pKa = 11.84 PYY350 pKa = 9.47 GTRR353 pKa = 11.84 RR354 pKa = 11.84 LYY356 pKa = 10.74 FDD358 pKa = 4.01 EE359 pKa = 4.46 SFNMSLHH366 pKa = 6.26 VGTQDD371 pKa = 5.12 SIIPWTVQTMGYY383 pKa = 10.64 LYY385 pKa = 7.5 MTTMSPNAYY394 pKa = 9.15 KK395 pKa = 10.51 YY396 pKa = 10.79 DD397 pKa = 3.09 IFAPVLIDD405 pKa = 3.82 APVTSVPFRR414 pKa = 11.84 FRR416 pKa = 11.84 SAA418 pKa = 2.95
Molecular weight: 49.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.849
IPC2_protein 5.931
IPC_protein 6.033
Toseland 6.532
ProMoST 6.44
Dawson 6.364
Bjellqvist 6.338
Wikipedia 6.389
Rodwell 6.364
Grimsley 6.722
Solomon 6.364
Lehninger 6.364
Nozaki 6.649
DTASelect 6.839
Thurlkill 6.868
EMBOSS 6.839
Sillero 6.751
Patrickios 3.185
IPC_peptide 6.389
IPC2_peptide 6.693
IPC2.peptide.svr19 6.677
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1440
418
1022
720.0
85.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.125 ± 0.118
2.014 ± 0.491
7.847 ± 0.645
5.556 ± 0.58
5.694 ± 0.534
4.306 ± 0.445
2.153 ± 0.778
7.083 ± 0.846
7.708 ± 1.358
8.819 ± 0.985
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.403 ± 0.086
6.667 ± 0.571
2.847 ± 0.678
2.708 ± 0.409
4.931 ± 0.068
7.431 ± 0.34
4.097 ± 1.209
5.069 ± 1.202
1.25 ± 0.308
7.292 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here