Gigaspora margarita giardia-like virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Giardiavirus; unclassified Giardiavirus

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2L0VZI1|A0A2L0VZI1_9VIRU RNA-directed RNA polymerase OS=Gigaspora margarita giardia-like virus 1 OX=2082658 PE=3 SV=1
MM1 pKa = 7.3SVDD4 pKa = 3.24EE5 pKa = 4.71MGLHH9 pKa = 7.09LSYY12 pKa = 10.67IKK14 pKa = 10.5CKK16 pKa = 10.69YY17 pKa = 9.94DD18 pKa = 3.67DD19 pKa = 4.33NEE21 pKa = 4.19MKK23 pKa = 10.37ISNLRR28 pKa = 11.84NLEE31 pKa = 4.02YY32 pKa = 10.81GKK34 pKa = 10.45EE35 pKa = 3.81FLMYY39 pKa = 9.53PQEE42 pKa = 4.03ISNMLKK48 pKa = 10.39LHH50 pKa = 6.56DD51 pKa = 4.32KK52 pKa = 10.93EE53 pKa = 5.76FIANHH58 pKa = 6.34KK59 pKa = 10.33IKK61 pKa = 9.76GTSLDD66 pKa = 3.46YY67 pKa = 11.53DD68 pKa = 3.6GFDD71 pKa = 3.41GVNIYY76 pKa = 10.29IEE78 pKa = 5.57DD79 pKa = 3.91IIEE82 pKa = 4.43DD83 pKa = 4.25EE84 pKa = 4.07NCILYY89 pKa = 9.58KK90 pKa = 10.28RR91 pKa = 11.84YY92 pKa = 9.21MEE94 pKa = 4.28GLSNIFCLKK103 pKa = 10.37DD104 pKa = 3.1SRR106 pKa = 11.84GRR108 pKa = 11.84YY109 pKa = 8.75NFSDD113 pKa = 3.38YY114 pKa = 11.28DD115 pKa = 3.98YY116 pKa = 10.83IIFLMAKK123 pKa = 9.57GYY125 pKa = 10.58GKK127 pKa = 8.62STFCKK132 pKa = 10.37DD133 pKa = 3.34NKK135 pKa = 10.35FCLDD139 pKa = 3.76LDD141 pKa = 4.49IILEE145 pKa = 4.16KK146 pKa = 10.62EE147 pKa = 4.2QAQGRR152 pKa = 11.84YY153 pKa = 6.71TLPDD157 pKa = 3.97DD158 pKa = 5.51LEE160 pKa = 4.41EE161 pKa = 4.8FDD163 pKa = 3.1WATYY167 pKa = 8.8NKK169 pKa = 10.02KK170 pKa = 9.94IATLYY175 pKa = 10.01SSQKK179 pKa = 6.83ITEE182 pKa = 4.91RR183 pKa = 11.84ILFSNNMTYY192 pKa = 10.16IDD194 pKa = 4.58AIEE197 pKa = 4.34GKK199 pKa = 9.77KK200 pKa = 10.41CVIKK204 pKa = 10.6IIGPFNPYY212 pKa = 10.29NSDD215 pKa = 3.93RR216 pKa = 11.84ISKK219 pKa = 9.75DD220 pKa = 3.08QYY222 pKa = 11.47LLASMNYY229 pKa = 9.58KK230 pKa = 9.86HH231 pKa = 6.25VQEE234 pKa = 4.54SDD236 pKa = 3.47CTLVADD242 pKa = 4.96FTLNPEE248 pKa = 4.4NIEE251 pKa = 4.31DD252 pKa = 4.32LLSGIHH258 pKa = 6.73IEE260 pKa = 4.07RR261 pKa = 11.84NYY263 pKa = 9.98KK264 pKa = 9.59KK265 pKa = 10.58EE266 pKa = 3.69VMRR269 pKa = 11.84VCKK272 pKa = 10.49LINEE276 pKa = 4.43KK277 pKa = 10.54KK278 pKa = 10.38KK279 pKa = 10.7NLDD282 pKa = 2.66KK283 pKa = 10.9DD284 pKa = 4.11YY285 pKa = 11.44YY286 pKa = 9.87FRR288 pKa = 11.84SYY290 pKa = 11.48NKK292 pKa = 9.28FVRR295 pKa = 11.84RR296 pKa = 11.84RR297 pKa = 11.84LMCKK301 pKa = 10.2RR302 pKa = 11.84ILYY305 pKa = 10.23DD306 pKa = 4.21DD307 pKa = 6.32LIPFYY312 pKa = 10.89DD313 pKa = 3.6EE314 pKa = 4.95SFSLMSDD321 pKa = 3.02ASISFKK327 pKa = 11.15NIFEE331 pKa = 4.34SKK333 pKa = 10.2YY334 pKa = 9.4YY335 pKa = 9.92PVLQGLRR342 pKa = 11.84TAQQYY347 pKa = 10.64EE348 pKa = 4.66DD349 pKa = 3.97YY350 pKa = 10.41LALIGSMNKK359 pKa = 9.89RR360 pKa = 11.84YY361 pKa = 9.54MIDD364 pKa = 3.08GDD366 pKa = 3.56NWIEE370 pKa = 3.95FCDD373 pKa = 4.73LGVCSGNYY381 pKa = 8.11TSYY384 pKa = 11.47DD385 pKa = 3.38KK386 pKa = 11.07EE387 pKa = 4.1KK388 pKa = 10.87CLEE391 pKa = 3.97KK392 pKa = 10.66LKK394 pKa = 11.04YY395 pKa = 7.16WTLEE399 pKa = 4.12YY400 pKa = 10.51KK401 pKa = 8.69EE402 pKa = 4.33HH403 pKa = 7.03CIDD406 pKa = 3.35GSEE409 pKa = 4.0PHH411 pKa = 6.3FLALFSEE418 pKa = 5.07GVNEE422 pKa = 4.26LFNNLEE428 pKa = 4.28VIPRR432 pKa = 11.84ADD434 pKa = 3.38DD435 pKa = 3.13QAYY438 pKa = 7.4TFRR441 pKa = 11.84EE442 pKa = 4.25YY443 pKa = 11.47LHH445 pKa = 6.78LPKK448 pKa = 10.22FWAVSGSTSIKK459 pKa = 10.26RR460 pKa = 11.84KK461 pKa = 9.56IKK463 pKa = 10.46VKK465 pKa = 7.55TTNGEE470 pKa = 4.14MKK472 pKa = 10.36VKK474 pKa = 7.78QTKK477 pKa = 8.09WVNAIFDD484 pKa = 4.08DD485 pKa = 4.58HH486 pKa = 7.6DD487 pKa = 3.68EE488 pKa = 4.09QYY490 pKa = 10.66TRR492 pKa = 11.84IMEE495 pKa = 4.31KK496 pKa = 10.49KK497 pKa = 8.55KK498 pKa = 8.5TADD501 pKa = 3.04YY502 pKa = 11.47KK503 pKa = 10.89MIVKK507 pKa = 9.3MGKK510 pKa = 8.03GKK512 pKa = 8.32TRR514 pKa = 11.84IVVSGDD520 pKa = 2.91LDD522 pKa = 3.96LYY524 pKa = 10.86AICDD528 pKa = 3.88YY529 pKa = 11.08IDD531 pKa = 3.46YY532 pKa = 10.6CVEE535 pKa = 4.05RR536 pKa = 11.84SIKK539 pKa = 10.11SCSISMLYY547 pKa = 10.6LDD549 pKa = 5.94DD550 pKa = 4.88DD551 pKa = 4.02QKK553 pKa = 11.92LDD555 pKa = 3.44FWMKK559 pKa = 10.25FCKK562 pKa = 10.3CDD564 pKa = 3.34DD565 pKa = 3.89SYY567 pKa = 11.96FYY569 pKa = 10.71MPLDD573 pKa = 3.44QSDD576 pKa = 3.71YY577 pKa = 11.36DD578 pKa = 3.55QMVNKK583 pKa = 10.3KK584 pKa = 8.25MVNIVLEE591 pKa = 4.03RR592 pKa = 11.84FLNFFLLHH600 pKa = 6.52AKK602 pKa = 10.52DD603 pKa = 3.6PVEE606 pKa = 3.99ARR608 pKa = 11.84SIFDD612 pKa = 3.38QVWRR616 pKa = 11.84CFEE619 pKa = 3.93YY620 pKa = 11.03GFIEE624 pKa = 4.47IEE626 pKa = 4.08DD627 pKa = 3.85RR628 pKa = 11.84KK629 pKa = 10.59EE630 pKa = 4.08LILNGVMSGYY640 pKa = 10.54KK641 pKa = 7.38WTAFFDD647 pKa = 4.03TVINASEE654 pKa = 3.96LHH656 pKa = 6.06VIRR659 pKa = 11.84TFLEE663 pKa = 4.09KK664 pKa = 10.53HH665 pKa = 5.7SLACIRR671 pKa = 11.84DD672 pKa = 3.59VNFQGDD678 pKa = 4.6DD679 pKa = 3.45VCLMLDD685 pKa = 3.4QDD687 pKa = 4.2VATCYY692 pKa = 10.91YY693 pKa = 10.28IFWLYY698 pKa = 10.85KK699 pKa = 9.81KK700 pKa = 10.54LGFLIHH706 pKa = 6.7ANKK709 pKa = 9.85FFLSNEE715 pKa = 3.7RR716 pKa = 11.84NEE718 pKa = 4.3YY719 pKa = 9.08LRR721 pKa = 11.84KK722 pKa = 10.03VSFKK726 pKa = 11.29GEE728 pKa = 3.56IRR730 pKa = 11.84GYY732 pKa = 8.49PARR735 pKa = 11.84NVISLLQPDD744 pKa = 4.66LMSIEE749 pKa = 4.94SDD751 pKa = 3.51DD752 pKa = 3.47QRR754 pKa = 11.84EE755 pKa = 4.1RR756 pKa = 11.84LTVYY760 pKa = 10.98LNGYY764 pKa = 7.79YY765 pKa = 10.48NFRR768 pKa = 11.84RR769 pKa = 11.84RR770 pKa = 11.84MRR772 pKa = 11.84SPISLDD778 pKa = 3.41DD779 pKa = 4.36KK780 pKa = 11.03IFYY783 pKa = 10.26PEE785 pKa = 3.87ADD787 pKa = 3.66YY788 pKa = 11.15YY789 pKa = 10.57IPNASKK795 pKa = 10.71LIHH798 pKa = 5.94VPKK801 pKa = 10.52SLGGFGFWPLVRR813 pKa = 11.84STKK816 pKa = 9.96YY817 pKa = 10.17RR818 pKa = 11.84FYY820 pKa = 11.27KK821 pKa = 8.82KK822 pKa = 10.13QSYY825 pKa = 8.62LQSVEE830 pKa = 3.95QDD832 pKa = 3.36DD833 pKa = 4.56NLMKK837 pKa = 10.88GIRR840 pKa = 11.84EE841 pKa = 3.99DD842 pKa = 3.49PLYY845 pKa = 9.73NTSYY849 pKa = 10.92DD850 pKa = 3.51GKK852 pKa = 10.57KK853 pKa = 10.19LFSILSPQAGRR864 pKa = 11.84HH865 pKa = 4.82RR866 pKa = 11.84NNIRR870 pKa = 11.84FGIRR874 pKa = 11.84EE875 pKa = 4.29LDD877 pKa = 3.41SYY879 pKa = 10.46ITLKK883 pKa = 11.06SMNLYY888 pKa = 10.49SDD890 pKa = 4.67RR891 pKa = 11.84LDD893 pKa = 3.81TLPSDD898 pKa = 3.04ISLYY902 pKa = 9.47SFRR905 pKa = 11.84NNSEE909 pKa = 3.96NNSLRR914 pKa = 11.84NSIFFDD920 pKa = 3.47SYY922 pKa = 11.03IRR924 pKa = 11.84SLDD927 pKa = 3.27ISDD930 pKa = 4.36LEE932 pKa = 4.54KK933 pKa = 10.7IFNFSFNINNASLSVVKK950 pKa = 10.72AIVNNEE956 pKa = 3.92LFSAPKK962 pKa = 8.27MFCYY966 pKa = 10.37SEE968 pKa = 4.5SSCSIVFNRR977 pKa = 11.84LIKK980 pKa = 10.7YY981 pKa = 10.5NMLFFMKK988 pKa = 10.29DD989 pKa = 2.74NYY991 pKa = 9.82LTLDD995 pKa = 3.26KK996 pKa = 10.68LKK998 pKa = 10.73RR999 pKa = 11.84IAFTMEE1005 pKa = 3.82LKK1007 pKa = 10.27FDD1009 pKa = 4.55KK1010 pKa = 10.72IMEE1013 pKa = 4.31KK1014 pKa = 10.62LYY1016 pKa = 10.82DD1017 pKa = 4.01CKK1019 pKa = 10.98DD1020 pKa = 3.45YY1021 pKa = 11.69SS1022 pKa = 3.44

Molecular weight:
120.99 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2L0VZI1|A0A2L0VZI1_9VIRU RNA-directed RNA polymerase OS=Gigaspora margarita giardia-like virus 1 OX=2082658 PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 9.22FTTTGEE8 pKa = 4.07NAVMGVVPPDD18 pKa = 3.87APDD21 pKa = 3.56TTSQRR26 pKa = 11.84YY27 pKa = 7.71NATVHH32 pKa = 6.87SNIEE36 pKa = 4.08DD37 pKa = 3.53VNGNRR42 pKa = 11.84VINDD46 pKa = 3.65TLSGTFHH53 pKa = 7.57AYY55 pKa = 10.41NYY57 pKa = 7.37LTNPLGQRR65 pKa = 11.84KK66 pKa = 9.09RR67 pKa = 11.84SVLTGDD73 pKa = 4.08EE74 pKa = 4.21NFMTYY79 pKa = 8.8DD80 pKa = 3.44TNIPQAGIYY89 pKa = 9.19EE90 pKa = 4.32RR91 pKa = 11.84LALMIGTHH99 pKa = 5.26QLVNKK104 pKa = 9.77VNIEE108 pKa = 3.9KK109 pKa = 10.65YY110 pKa = 10.01IEE112 pKa = 4.39SNHH115 pKa = 5.75FNHH118 pKa = 6.01VVIGKK123 pKa = 9.33HH124 pKa = 5.08MASCLGKK131 pKa = 9.83MGDD134 pKa = 3.99VVLQQYY140 pKa = 8.59DD141 pKa = 3.13IPMCYY146 pKa = 10.03LLEE149 pKa = 5.28PIHH152 pKa = 6.04GTQNHH157 pKa = 5.71YY158 pKa = 11.33KK159 pKa = 8.44NTLLQRR165 pKa = 11.84YY166 pKa = 6.92YY167 pKa = 11.02NRR169 pKa = 11.84EE170 pKa = 3.86HH171 pKa = 6.59PFSRR175 pKa = 11.84EE176 pKa = 3.68MVSVYY181 pKa = 11.48DD182 pKa = 3.97MMSFDD187 pKa = 4.89YY188 pKa = 11.03LNKK191 pKa = 10.46LKK193 pKa = 10.07WDD195 pKa = 3.35EE196 pKa = 4.21RR197 pKa = 11.84LWFSGQYY204 pKa = 10.63SNPQGSGLANYY215 pKa = 10.28LSDD218 pKa = 3.59VYY220 pKa = 10.58RR221 pKa = 11.84YY222 pKa = 9.94SKK224 pKa = 8.32WHH226 pKa = 6.69RR227 pKa = 11.84EE228 pKa = 3.87YY229 pKa = 10.62STIGYY234 pKa = 7.54DD235 pKa = 3.8TNDD238 pKa = 3.49HH239 pKa = 6.19VSDD242 pKa = 5.11LSFIDD247 pKa = 3.75INWEE251 pKa = 3.9KK252 pKa = 10.5HH253 pKa = 4.95VKK255 pKa = 9.56DD256 pKa = 3.41QIYY259 pKa = 10.4YY260 pKa = 7.76RR261 pKa = 11.84TPDD264 pKa = 3.7DD265 pKa = 3.23QHH267 pKa = 7.38VIRR270 pKa = 11.84WVKK273 pKa = 9.51KK274 pKa = 7.76WVYY277 pKa = 9.84EE278 pKa = 3.99LNEE281 pKa = 4.48LEE283 pKa = 4.26SLDD286 pKa = 3.37WTRR289 pKa = 11.84LYY291 pKa = 11.2GVDD294 pKa = 3.58NNVDD298 pKa = 3.54YY299 pKa = 11.62VFGHH303 pKa = 6.02NNRR306 pKa = 11.84CIKK309 pKa = 10.06VVNFIGKK316 pKa = 9.13EE317 pKa = 3.92NQSYY321 pKa = 11.07NSFFKK326 pKa = 10.91FMTEE330 pKa = 4.23CNLHH334 pKa = 6.87FYY336 pKa = 7.88TKK338 pKa = 9.79PVSIKK343 pKa = 10.63SYY345 pKa = 10.67DD346 pKa = 3.34PRR348 pKa = 11.84PYY350 pKa = 9.47GTRR353 pKa = 11.84RR354 pKa = 11.84LYY356 pKa = 10.74FDD358 pKa = 4.01EE359 pKa = 4.46SFNMSLHH366 pKa = 6.26VGTQDD371 pKa = 5.12SIIPWTVQTMGYY383 pKa = 10.64LYY385 pKa = 7.5MTTMSPNAYY394 pKa = 9.15KK395 pKa = 10.51YY396 pKa = 10.79DD397 pKa = 3.09IFAPVLIDD405 pKa = 3.82APVTSVPFRR414 pKa = 11.84FRR416 pKa = 11.84SAA418 pKa = 2.95

Molecular weight:
49.03 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1440

418

1022

720.0

85.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.125 ± 0.118

2.014 ± 0.491

7.847 ± 0.645

5.556 ± 0.58

5.694 ± 0.534

4.306 ± 0.445

2.153 ± 0.778

7.083 ± 0.846

7.708 ± 1.358

8.819 ± 0.985

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.403 ± 0.086

6.667 ± 0.571

2.847 ± 0.678

2.708 ± 0.409

4.931 ± 0.068

7.431 ± 0.34

4.097 ± 1.209

5.069 ± 1.202

1.25 ± 0.308

7.292 ± 0.28

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski