Candidatus Pantoea edessiphila
Average proteome isoelectric point is 7.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 689 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P5SWX7|A0A2P5SWX7_9GAMM 50S ribosomal protein L35 OS=Candidatus Pantoea edessiphila OX=2044610 GN=rpmI PE=3 SV=1
MM1 pKa = 7.65 SDD3 pKa = 2.68 IEE5 pKa = 4.12 QRR7 pKa = 11.84 VKK9 pKa = 10.82 KK10 pKa = 10.27 IICEE14 pKa = 3.81 QLGVKK19 pKa = 9.6 EE20 pKa = 4.87 DD21 pKa = 3.79 EE22 pKa = 4.64 VTNSASFVDD31 pKa = 5.15 DD32 pKa = 5.07 LGADD36 pKa = 3.42 SLDD39 pKa = 3.64 TVEE42 pKa = 5.9 LVMALEE48 pKa = 4.51 EE49 pKa = 4.26 EE50 pKa = 4.66 FDD52 pKa = 3.79 TEE54 pKa = 4.56 IPDD57 pKa = 3.56 EE58 pKa = 4.18 EE59 pKa = 4.49 AEE61 pKa = 5.03 KK62 pKa = 9.69 ITTVQAAIDD71 pKa = 4.16 YY72 pKa = 11.09 INDD75 pKa = 3.53 HH76 pKa = 6.58 KK77 pKa = 11.52 NN78 pKa = 2.85
Molecular weight: 8.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.271
IPC2_protein 3.872
IPC_protein 3.808
Toseland 3.63
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.935
Patrickios 3.643
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.85
Protein with the highest isoelectric point:
>tr|A0A2P5SVY9|A0A2P5SVY9_9GAMM Sulfurtransferase complex subunit TusD OS=Candidatus Pantoea edessiphila OX=2044610 GN=CRV10_02520 PE=3 SV=1
MM1 pKa = 7.25 ATINQLVRR9 pKa = 11.84 KK10 pKa = 8.62 PRR12 pKa = 11.84 VRR14 pKa = 11.84 KK15 pKa = 8.27 LTKK18 pKa = 10.4 SNVPALEE25 pKa = 4.21 ACPQKK30 pKa = 10.67 RR31 pKa = 11.84 GVCTRR36 pKa = 11.84 VYY38 pKa = 7.93 TTTPKK43 pKa = 10.54 KK44 pKa = 10.26 PNSALRR50 pKa = 11.84 KK51 pKa = 7.38 VCRR54 pKa = 11.84 VRR56 pKa = 11.84 LTNNFEE62 pKa = 3.91 VTSYY66 pKa = 10.76 IGGEE70 pKa = 3.94 GHH72 pKa = 6.6 NLQEE76 pKa = 4.28 HH77 pKa = 5.6 SVVLIRR83 pKa = 11.84 GGRR86 pKa = 11.84 VKK88 pKa = 10.7 DD89 pKa = 3.73 LPGVRR94 pKa = 11.84 YY95 pKa = 8.13 HH96 pKa = 6.92 TIRR99 pKa = 11.84 GALDD103 pKa = 3.24 CAGVKK108 pKa = 9.53 DD109 pKa = 4.11 RR110 pKa = 11.84 KK111 pKa = 8.34 QARR114 pKa = 11.84 SKK116 pKa = 11.5 YY117 pKa = 9.98 GMKK120 pKa = 10.16 KK121 pKa = 9.94 PKK123 pKa = 10.13 SS124 pKa = 3.58
Molecular weight: 13.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.897
IPC_protein 10.643
Toseland 10.994
ProMoST 10.672
Dawson 11.052
Bjellqvist 10.76
Wikipedia 11.257
Rodwell 11.389
Grimsley 11.096
Solomon 11.199
Lehninger 11.169
Nozaki 10.979
DTASelect 10.745
Thurlkill 10.979
EMBOSS 11.403
Sillero 10.994
Patrickios 11.111
IPC_peptide 11.213
IPC2_peptide 9.809
IPC2.peptide.svr19 8.457
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
689
0
689
229877
38
1409
333.6
37.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.369 ± 0.067
1.387 ± 0.03
4.658 ± 0.059
5.098 ± 0.078
4.217 ± 0.067
5.896 ± 0.074
2.173 ± 0.034
11.26 ± 0.094
7.819 ± 0.081
10.132 ± 0.101
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.391 ± 0.038
7.25 ± 0.104
3.326 ± 0.044
3.433 ± 0.044
4.221 ± 0.065
6.571 ± 0.058
4.806 ± 0.051
5.358 ± 0.062
1.024 ± 0.032
3.611 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here