Pseudomonas phage vB_PaeM_PS119XW

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phikzvirus; unclassified Phikzvirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1K7B2|A0A5C1K7B2_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeM_PS119XW OX=2601632 PE=4 SV=1
MM1 pKa = 8.01PDD3 pKa = 3.65NYY5 pKa = 10.82LADD8 pKa = 3.71ATEE11 pKa = 5.08RR12 pKa = 11.84IAPHH16 pKa = 5.55TWVLSPDD23 pKa = 3.98DD24 pKa = 3.57PHH26 pKa = 7.02EE27 pKa = 4.16ISGNLEE33 pKa = 3.66LQGPVVEE40 pKa = 4.94TIINYY45 pKa = 9.28AHH47 pKa = 7.28EE48 pKa = 5.94DD49 pKa = 3.71YY50 pKa = 11.09LPSLEE55 pKa = 4.07EE56 pKa = 4.05WLAKK60 pKa = 10.1AAA62 pKa = 4.28

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1K7W2|A0A5C1K7W2_9CAUD DNA ligase (NAD(+)) OS=Pseudomonas phage vB_PaeM_PS119XW OX=2601632 PE=3 SV=1
MM1 pKa = 7.31SRR3 pKa = 11.84SNHH6 pKa = 5.98SKK8 pKa = 9.4HH9 pKa = 5.31QQSKK13 pKa = 8.26EE14 pKa = 3.7FWSRR18 pKa = 11.84RR19 pKa = 11.84PMRR22 pKa = 11.84YY23 pKa = 7.58FTSCKK28 pKa = 7.0WAKK31 pKa = 9.54VKK33 pKa = 9.76CHH35 pKa = 6.54RR36 pKa = 11.84IEE38 pKa = 3.96RR39 pKa = 11.84AQARR43 pKa = 11.84DD44 pKa = 3.65LVRR47 pKa = 11.84KK48 pKa = 9.14EE49 pKa = 3.59VDD51 pKa = 3.05KK52 pKa = 11.62LL53 pKa = 3.61

Molecular weight: 6.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

389

0

389

93542

31

2256

240.5

27.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.013 ± 0.154

0.916 ± 0.046

6.42 ± 0.089

6.904 ± 0.175

4.123 ± 0.069

6.425 ± 0.17

2.056 ± 0.068

6.499 ± 0.095

5.851 ± 0.116

8.332 ± 0.112

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.862 ± 0.069

5.233 ± 0.096

4.073 ± 0.092

3.552 ± 0.093

5.29 ± 0.105

5.826 ± 0.092

6.091 ± 0.094

7.176 ± 0.106

1.372 ± 0.048

3.986 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski