Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Acetivibrio; Acetivibrio thermocellus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P26208|BGLA_HUNT2 Beta-glucosidase A OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglA PE=1 SV=1
MM1 pKa = 7.44KK2 pKa = 10.38GVTIFGNFTVLEE14 pKa = 4.16TFMFNDD20 pKa = 3.8LQIDD24 pKa = 3.44KK25 pKa = 11.07GFDD28 pKa = 3.03EE29 pKa = 5.26RR30 pKa = 11.84LDD32 pKa = 3.0IFYY35 pKa = 10.65RR36 pKa = 11.84VNLYY40 pKa = 9.96IEE42 pKa = 4.36NLEE45 pKa = 4.69DD46 pKa = 5.93DD47 pKa = 4.3INDD50 pKa = 3.89TFIDD54 pKa = 4.11CYY56 pKa = 10.67YY57 pKa = 10.93RR58 pKa = 11.84SINDD62 pKa = 3.21LMEE65 pKa = 5.67DD66 pKa = 3.75IKK68 pKa = 11.08CYY70 pKa = 10.66SINQTT75 pKa = 3.41

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A3DI22|FTHS_HUNT2 Formate--tetrahydrofolate ligase OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=fhs PE=3 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.46KK9 pKa = 8.28RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.76RR21 pKa = 11.84MKK23 pKa = 8.31TANGRR28 pKa = 11.84KK29 pKa = 7.52VLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3105

0

3105

1033401

30

6885

332.8

37.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.415 ± 0.047

1.171 ± 0.019

5.735 ± 0.029

7.6 ± 0.046

4.262 ± 0.032

6.719 ± 0.041

1.408 ± 0.018

8.519 ± 0.042

8.052 ± 0.041

8.652 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.524 ± 0.022

5.387 ± 0.031

3.404 ± 0.039

2.551 ± 0.027

4.278 ± 0.032

6.051 ± 0.041

5.031 ± 0.05

7.16 ± 0.047

0.874 ± 0.016

4.206 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski