Amino acid dipepetide frequency for Methylophaga frappieri (strain ATCC BAA-2434 / DSM 25690 / JAM7)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.825AlaAla: 9.825 ± 0.147
0.943AlaCys: 0.943 ± 0.036
6.346AlaAsp: 6.346 ± 0.082
6.567AlaGlu: 6.567 ± 0.112
3.441AlaPhe: 3.441 ± 0.073
7.297AlaGly: 7.297 ± 0.113
1.878AlaHis: 1.878 ± 0.058
6.084AlaIle: 6.084 ± 0.101
4.013AlaLys: 4.013 ± 0.086
10.716AlaLeu: 10.716 ± 0.14
2.907AlaMet: 2.907 ± 0.069
3.52AlaAsn: 3.52 ± 0.083
3.139AlaPro: 3.139 ± 0.068
4.144AlaGln: 4.144 ± 0.076
4.793AlaArg: 4.793 ± 0.088
5.336AlaSer: 5.336 ± 0.099
4.898AlaThr: 4.898 ± 0.101
6.487AlaVal: 6.487 ± 0.099
1.097AlaTrp: 1.097 ± 0.04
2.504AlaTyr: 2.504 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.677CysAla: 0.677 ± 0.028
0.135CysCys: 0.135 ± 0.014
0.523CysAsp: 0.523 ± 0.024
0.552CysGlu: 0.552 ± 0.029
0.392CysPhe: 0.392 ± 0.024
0.78CysGly: 0.78 ± 0.032
0.36CysHis: 0.36 ± 0.023
0.481CysIle: 0.481 ± 0.024
0.255CysLys: 0.255 ± 0.019
0.945CysLeu: 0.945 ± 0.035
0.15CysMet: 0.15 ± 0.014
0.281CysAsn: 0.281 ± 0.021
0.488CysPro: 0.488 ± 0.027
0.587CysGln: 0.587 ± 0.029
0.623CysArg: 0.623 ± 0.037
0.506CysSer: 0.506 ± 0.027
0.345CysThr: 0.345 ± 0.023
0.547CysVal: 0.547 ± 0.026
0.109CysTrp: 0.109 ± 0.012
0.263CysTyr: 0.263 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
5.642AspAla: 5.642 ± 0.084
0.526AspCys: 0.526 ± 0.029
3.642AspAsp: 3.642 ± 0.113
3.668AspGlu: 3.668 ± 0.073
2.521AspPhe: 2.521 ± 0.072
4.138AspGly: 4.138 ± 0.131
1.202AspHis: 1.202 ± 0.043
3.913AspIle: 3.913 ± 0.083
2.778AspLys: 2.778 ± 0.055
5.805AspLeu: 5.805 ± 0.094
1.521AspMet: 1.521 ± 0.047
2.48AspAsn: 2.48 ± 0.063
2.453AspPro: 2.453 ± 0.059
2.357AspGln: 2.357 ± 0.06
2.889AspArg: 2.889 ± 0.069
3.354AspSer: 3.354 ± 0.082
3.118AspThr: 3.118 ± 0.082
3.857AspVal: 3.857 ± 0.072
1.085AspTrp: 1.085 ± 0.038
2.091AspTyr: 2.091 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
5.78GluAla: 5.78 ± 0.09
0.376GluCys: 0.376 ± 0.021
2.639GluAsp: 2.639 ± 0.062
2.814GluGlu: 2.814 ± 0.072
1.985GluPhe: 1.985 ± 0.052
3.141GluGly: 3.141 ± 0.072
1.293GluHis: 1.293 ± 0.043
3.89GluIle: 3.89 ± 0.068
3.117GluLys: 3.117 ± 0.061
5.844GluLeu: 5.844 ± 0.086
1.724GluMet: 1.724 ± 0.048
2.575GluAsn: 2.575 ± 0.06
2.238GluPro: 2.238 ± 0.056
4.033GluGln: 4.033 ± 0.073
3.383GluArg: 3.383 ± 0.073
3.496GluSer: 3.496 ± 0.074
3.753GluThr: 3.753 ± 0.072
3.973GluVal: 3.973 ± 0.076
0.68GluTrp: 0.68 ± 0.029
1.399GluTyr: 1.399 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
3.532PheAla: 3.532 ± 0.067
0.44PheCys: 0.44 ± 0.023
2.735PheAsp: 2.735 ± 0.068
2.248PheGlu: 2.248 ± 0.044
1.625PhePhe: 1.625 ± 0.049
2.907PheGly: 2.907 ± 0.068
0.782PheHis: 0.782 ± 0.03
2.357PheIle: 2.357 ± 0.053
1.389PheLys: 1.389 ± 0.044
3.48PheLeu: 3.48 ± 0.074
0.966PheMet: 0.966 ± 0.034
1.677PheAsn: 1.677 ± 0.051
1.399PhePro: 1.399 ± 0.045
1.408PheGln: 1.408 ± 0.039
1.881PheArg: 1.881 ± 0.041
2.906PheSer: 2.906 ± 0.055
2.246PheThr: 2.246 ± 0.063
2.466PheVal: 2.466 ± 0.059
0.52PheTrp: 0.52 ± 0.026
1.261PheTyr: 1.261 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
5.381GlyAla: 5.381 ± 0.099
0.769GlyCys: 0.769 ± 0.034
3.972GlyAsp: 3.972 ± 0.122
4.373GlyGlu: 4.373 ± 0.074
3.174GlyPhe: 3.174 ± 0.058
5.119GlyGly: 5.119 ± 0.151
1.695GlyHis: 1.695 ± 0.047
4.495GlyIle: 4.495 ± 0.086
3.43GlyLys: 3.43 ± 0.065
7.557GlyLeu: 7.557 ± 0.109
1.927GlyMet: 1.927 ± 0.054
2.646GlyAsn: 2.646 ± 0.09
1.894GlyPro: 1.894 ± 0.05
3.392GlyGln: 3.392 ± 0.074
3.679GlyArg: 3.679 ± 0.07
4.039GlySer: 4.039 ± 0.096
3.423GlyThr: 3.423 ± 0.112
4.922GlyVal: 4.922 ± 0.085
1.072GlyTrp: 1.072 ± 0.038
2.292GlyTyr: 2.292 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
2.02HisAla: 2.02 ± 0.053
0.358HisCys: 0.358 ± 0.03
1.385HisAsp: 1.385 ± 0.036
1.172HisGlu: 1.172 ± 0.04
1.055HisPhe: 1.055 ± 0.038
1.638HisGly: 1.638 ± 0.05
0.83HisHis: 0.83 ± 0.036
1.408HisIle: 1.408 ± 0.05
0.776HisLys: 0.776 ± 0.031
2.363HisLeu: 2.363 ± 0.059
0.451HisMet: 0.451 ± 0.022
0.818HisAsn: 0.818 ± 0.034
1.309HisPro: 1.309 ± 0.044
1.461HisGln: 1.461 ± 0.053
1.354HisArg: 1.354 ± 0.044
1.177HisSer: 1.177 ± 0.042
1.123HisThr: 1.123 ± 0.034
1.354HisVal: 1.354 ± 0.045
0.427HisTrp: 0.427 ± 0.025
0.91HisTyr: 0.91 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.333IleAla: 6.333 ± 0.096
0.565IleCys: 0.565 ± 0.024
4.269IleAsp: 4.269 ± 0.08
4.181IleGlu: 4.181 ± 0.074
1.979IlePhe: 1.979 ± 0.052
4.475IleGly: 4.475 ± 0.085
1.321IleHis: 1.321 ± 0.042
3.32IleIle: 3.32 ± 0.066
2.48IleLys: 2.48 ± 0.061
5.241IleLeu: 5.241 ± 0.093
1.262IleMet: 1.262 ± 0.041
2.718IleAsn: 2.718 ± 0.063
2.511IlePro: 2.511 ± 0.06
2.487IleGln: 2.487 ± 0.062
3.437IleArg: 3.437 ± 0.069
4.093IleSer: 4.093 ± 0.073
3.654IleThr: 3.654 ± 0.091
3.765IleVal: 3.765 ± 0.08
0.713IleTrp: 0.713 ± 0.037
1.56IleTyr: 1.56 ± 0.042
0.0IleXaa: 0.0 ± 0.0
Lys
4.049LysAla: 4.049 ± 0.082
0.233LysCys: 0.233 ± 0.02
1.854LysAsp: 1.854 ± 0.052
1.933LysGlu: 1.933 ± 0.059
1.094LysPhe: 1.094 ± 0.038
2.372LysGly: 2.372 ± 0.06
0.981LysHis: 0.981 ± 0.037
2.345LysIle: 2.345 ± 0.06
2.16LysLys: 2.16 ± 0.071
4.409LysLeu: 4.409 ± 0.086
1.108LysMet: 1.108 ± 0.047
1.631LysAsn: 1.631 ± 0.047
2.133LysPro: 2.133 ± 0.061
2.743LysGln: 2.743 ± 0.061
2.578LysArg: 2.578 ± 0.068
2.538LysSer: 2.538 ± 0.053
2.74LysThr: 2.74 ± 0.06
2.669LysVal: 2.669 ± 0.065
0.45LysTrp: 0.45 ± 0.03
0.894LysTyr: 0.894 ± 0.037
0.0LysXaa: 0.0 ± 0.0
Leu
11.13LeuAla: 11.13 ± 0.144
0.944LeuCys: 0.944 ± 0.037
6.053LeuAsp: 6.053 ± 0.09
5.517LeuGlu: 5.517 ± 0.097
4.221LeuPhe: 4.221 ± 0.092
6.678LeuGly: 6.678 ± 0.114
2.314LeuHis: 2.314 ± 0.05
6.68LeuIle: 6.68 ± 0.106
4.4LeuLys: 4.4 ± 0.081
11.413LeuLeu: 11.413 ± 0.178
2.707LeuMet: 2.707 ± 0.056
4.21LeuAsn: 4.21 ± 0.07
5.702LeuPro: 5.702 ± 0.084
5.123LeuGln: 5.123 ± 0.092
5.732LeuArg: 5.732 ± 0.097
7.647LeuSer: 7.647 ± 0.099
6.755LeuThr: 6.755 ± 0.094
6.512LeuVal: 6.512 ± 0.102
1.204LeuTrp: 1.204 ± 0.043
2.505LeuTyr: 2.505 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
2.841MetAla: 2.841 ± 0.059
0.163MetCys: 0.163 ± 0.016
1.243MetAsp: 1.243 ± 0.041
1.066MetGlu: 1.066 ± 0.041
0.761MetPhe: 0.761 ± 0.031
1.622MetGly: 1.622 ± 0.052
0.559MetHis: 0.559 ± 0.025
1.421MetIle: 1.421 ± 0.048
1.078MetLys: 1.078 ± 0.037
2.812MetLeu: 2.812 ± 0.073
0.776MetMet: 0.776 ± 0.034
0.927MetAsn: 0.927 ± 0.032
1.33MetPro: 1.33 ± 0.044
1.507MetGln: 1.507 ± 0.045
1.498MetArg: 1.498 ± 0.043
1.677MetSer: 1.677 ± 0.045
1.801MetThr: 1.801 ± 0.051
1.702MetVal: 1.702 ± 0.045
0.224MetTrp: 0.224 ± 0.015
0.377MetTyr: 0.377 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.659AsnAla: 3.659 ± 0.072
0.306AsnCys: 0.306 ± 0.019
2.185AsnAsp: 2.185 ± 0.083
1.979AsnGlu: 1.979 ± 0.051
1.205AsnPhe: 1.205 ± 0.038
2.729AsnGly: 2.729 ± 0.081
0.967AsnHis: 0.967 ± 0.037
2.228AsnIle: 2.228 ± 0.056
1.539AsnLys: 1.539 ± 0.046
3.983AsnLeu: 3.983 ± 0.078
0.89AsnMet: 0.89 ± 0.032
1.59AsnAsn: 1.59 ± 0.058
2.04AsnPro: 2.04 ± 0.058
2.183AsnGln: 2.183 ± 0.048
2.191AsnArg: 2.191 ± 0.059
2.053AsnSer: 2.053 ± 0.064
2.148AsnThr: 2.148 ± 0.066
2.344AsnVal: 2.344 ± 0.06
0.619AsnTrp: 0.619 ± 0.027
1.129AsnTyr: 1.129 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
4.337ProAla: 4.337 ± 0.078
0.318ProCys: 0.318 ± 0.021
3.218ProAsp: 3.218 ± 0.058
3.527ProGlu: 3.527 ± 0.073
1.669ProPhe: 1.669 ± 0.048
3.039ProGly: 3.039 ± 0.069
0.835ProHis: 0.835 ± 0.031
2.256ProIle: 2.256 ± 0.055
1.568ProLys: 1.568 ± 0.047
4.35ProLeu: 4.35 ± 0.087
1.03ProMet: 1.03 ± 0.033
1.475ProAsn: 1.475 ± 0.044
1.614ProPro: 1.614 ± 0.056
1.768ProGln: 1.768 ± 0.047
1.797ProArg: 1.797 ± 0.052
2.325ProSer: 2.325 ± 0.054
2.187ProThr: 2.187 ± 0.053
3.935ProVal: 3.935 ± 0.073
0.54ProTrp: 0.54 ± 0.027
1.171ProTyr: 1.171 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
5.393GlnAla: 5.393 ± 0.096
0.425GlnCys: 0.425 ± 0.025
2.279GlnAsp: 2.279 ± 0.064
2.271GlnGlu: 2.271 ± 0.06
1.878GlnPhe: 1.878 ± 0.049
3.013GlnGly: 3.013 ± 0.062
1.57GlnHis: 1.57 ± 0.047
3.066GlnIle: 3.066 ± 0.064
2.02GlnLys: 2.02 ± 0.052
6.013GlnLeu: 6.013 ± 0.108
1.103GlnMet: 1.103 ± 0.035
1.695GlnAsn: 1.695 ± 0.045
2.43GlnPro: 2.43 ± 0.057
4.434GlnGln: 4.434 ± 0.111
3.489GlnArg: 3.489 ± 0.079
3.167GlnSer: 3.167 ± 0.069
2.974GlnThr: 2.974 ± 0.056
3.386GlnVal: 3.386 ± 0.073
0.749GlnTrp: 0.749 ± 0.034
1.336GlnTyr: 1.336 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
4.186ArgAla: 4.186 ± 0.075
0.489ArgCys: 0.489 ± 0.026
3.161ArgAsp: 3.161 ± 0.067
3.452ArgGlu: 3.452 ± 0.08
2.509ArgPhe: 2.509 ± 0.056
3.149ArgGly: 3.149 ± 0.058
1.671ArgHis: 1.671 ± 0.045
3.377ArgIle: 3.377 ± 0.079
2.173ArgLys: 2.173 ± 0.061
6.577ArgLeu: 6.577 ± 0.096
1.389ArgMet: 1.389 ± 0.039
1.902ArgAsn: 1.902 ± 0.046
2.136ArgPro: 2.136 ± 0.06
3.706ArgGln: 3.706 ± 0.081
3.476ArgArg: 3.476 ± 0.066
2.947ArgSer: 2.947 ± 0.071
2.277ArgThr: 2.277 ± 0.049
3.482ArgVal: 3.482 ± 0.064
0.869ArgTrp: 0.869 ± 0.033
2.087ArgTyr: 2.087 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
5.97SerAla: 5.97 ± 0.09
0.52SerCys: 0.52 ± 0.026
3.68SerAsp: 3.68 ± 0.084
3.649SerGlu: 3.649 ± 0.071
2.246SerPhe: 2.246 ± 0.052
5.277SerGly: 5.277 ± 0.117
1.46SerHis: 1.46 ± 0.042
3.168SerIle: 3.168 ± 0.058
2.142SerLys: 2.142 ± 0.052
6.875SerLeu: 6.875 ± 0.085
1.525SerMet: 1.525 ± 0.043
2.011SerAsn: 2.011 ± 0.064
2.591SerPro: 2.591 ± 0.054
3.023SerGln: 3.023 ± 0.067
3.44SerArg: 3.44 ± 0.066
3.515SerSer: 3.515 ± 0.081
2.978SerThr: 2.978 ± 0.072
4.239SerVal: 4.239 ± 0.079
0.863SerTrp: 0.863 ± 0.033
1.669SerTyr: 1.669 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
5.521ThrAla: 5.521 ± 0.093
0.389ThrCys: 0.389 ± 0.023
3.441ThrAsp: 3.441 ± 0.076
3.301ThrGlu: 3.301 ± 0.072
1.95ThrPhe: 1.95 ± 0.063
4.787ThrGly: 4.787 ± 0.102
1.236ThrHis: 1.236 ± 0.046
3.227ThrIle: 3.227 ± 0.078
1.663ThrLys: 1.663 ± 0.048
6.896ThrLeu: 6.896 ± 0.099
1.25ThrMet: 1.25 ± 0.036
1.778ThrAsn: 1.778 ± 0.055
2.941ThrPro: 2.941 ± 0.065
2.41ThrGln: 2.41 ± 0.051
2.615ThrArg: 2.615 ± 0.058
3.066ThrSer: 3.066 ± 0.066
2.961ThrThr: 2.961 ± 0.072
4.07ThrVal: 4.07 ± 0.1
0.581ThrTrp: 0.581 ± 0.03
1.301ThrTyr: 1.301 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
6.304ValAla: 6.304 ± 0.093
0.631ValCys: 0.631 ± 0.029
4.222ValAsp: 4.222 ± 0.077
3.878ValGlu: 3.878 ± 0.081
2.718ValPhe: 2.718 ± 0.072
4.362ValGly: 4.362 ± 0.107
1.26ValHis: 1.26 ± 0.045
4.655ValIle: 4.655 ± 0.089
2.675ValLys: 2.675 ± 0.064
6.779ValLeu: 6.779 ± 0.11
1.968ValMet: 1.968 ± 0.05
2.792ValAsn: 2.792 ± 0.06
2.712ValPro: 2.712 ± 0.062
2.428ValGln: 2.428 ± 0.059
3.351ValArg: 3.351 ± 0.076
4.677ValSer: 4.677 ± 0.079
4.216ValThr: 4.216 ± 0.084
4.962ValVal: 4.962 ± 0.096
0.832ValTrp: 0.832 ± 0.039
1.668ValTyr: 1.668 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.92TrpAla: 0.92 ± 0.039
0.146TrpCys: 0.146 ± 0.014
0.547TrpAsp: 0.547 ± 0.028
0.519TrpGlu: 0.519 ± 0.024
0.564TrpPhe: 0.564 ± 0.026
0.736TrpGly: 0.736 ± 0.029
0.438TrpHis: 0.438 ± 0.027
0.684TrpIle: 0.684 ± 0.03
0.372TrpLys: 0.372 ± 0.021
2.183TrpLeu: 2.183 ± 0.057
0.309TrpMet: 0.309 ± 0.02
0.382TrpAsn: 0.382 ± 0.022
0.598TrpPro: 0.598 ± 0.027
1.37TrpGln: 1.37 ± 0.051
0.891TrpArg: 0.891 ± 0.037
0.736TrpSer: 0.736 ± 0.03
0.531TrpThr: 0.531 ± 0.026
0.8TrpVal: 0.8 ± 0.035
0.217TrpTrp: 0.217 ± 0.015
0.352TrpTyr: 0.352 ± 0.034
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.435TyrAla: 2.435 ± 0.054
0.323TyrCys: 0.323 ± 0.02
1.647TyrAsp: 1.647 ± 0.049
1.466TyrGlu: 1.466 ± 0.049
1.239TyrPhe: 1.239 ± 0.036
1.993TyrGly: 1.993 ± 0.054
0.739TyrHis: 0.739 ± 0.032
1.309TyrIle: 1.309 ± 0.044
0.887TyrLys: 0.887 ± 0.037
3.208TyrLeu: 3.208 ± 0.055
0.421TyrMet: 0.421 ± 0.021
0.854TyrAsn: 0.854 ± 0.033
1.302TyrPro: 1.302 ± 0.04
2.125TyrGln: 2.125 ± 0.057
1.915TyrArg: 1.915 ± 0.055
1.586TyrSer: 1.586 ± 0.046
1.257TyrThr: 1.257 ± 0.042
1.616TyrVal: 1.616 ± 0.042
0.446TyrTrp: 0.446 ± 0.023
0.897TyrTyr: 0.897 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2689 proteins (835832 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski