Rhizobium phage RHEph10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7TKU9|L7TKU9_9CAUD Uncharacterized protein OS=Rhizobium phage RHEph10 OX=1220717 GN=RHEph10_gp100 PE=4 SV=1
MM1 pKa = 7.88WIHH4 pKa = 6.23LTDD7 pKa = 4.36AEE9 pKa = 4.44TSLLQEE15 pKa = 4.41ALRR18 pKa = 11.84GNCTDD23 pKa = 3.81IPGHH27 pKa = 6.0EE28 pKa = 4.31NDD30 pKa = 4.58HH31 pKa = 7.1DD32 pKa = 4.0RR33 pKa = 11.84LFDD36 pKa = 3.71KK37 pKa = 11.47VEE39 pKa = 3.73AAAARR44 pKa = 11.84QRR46 pKa = 11.84EE47 pKa = 4.25PEE49 pKa = 4.92AILVSGTIVYY59 pKa = 10.71GDD61 pKa = 4.08DD62 pKa = 3.54CSGVQSVAFEE72 pKa = 4.51RR73 pKa = 11.84YY74 pKa = 8.9DD75 pKa = 3.43ALTDD79 pKa = 3.76GNINYY84 pKa = 8.13DD85 pKa = 3.9ANTIMCRR92 pKa = 11.84VDD94 pKa = 3.22DD95 pKa = 4.26GAYY98 pKa = 9.31VMGWIYY104 pKa = 10.41VSNMEE109 pKa = 5.11IEE111 pKa = 5.57DD112 pKa = 3.67VDD114 pKa = 3.68QSS116 pKa = 3.52

Molecular weight: 12.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7TNG6|L7TNG6_9CAUD DNA polymerase III subunit alpha OS=Rhizobium phage RHEph10 OX=1220717 GN=RHEph10_gp048 PE=4 SV=1
MM1 pKa = 7.35SKK3 pKa = 9.62SHH5 pKa = 7.22KK6 pKa = 10.2LRR8 pKa = 11.84AEE10 pKa = 3.52LRR12 pKa = 11.84EE13 pKa = 4.23AYY15 pKa = 10.62NLVAPSGATKK25 pKa = 9.99TIIGKK30 pKa = 9.39ALWLHH35 pKa = 6.1LRR37 pKa = 11.84RR38 pKa = 11.84LGDD41 pKa = 3.41DD42 pKa = 3.4MRR44 pKa = 11.84WYY46 pKa = 10.58AIRR49 pKa = 11.84RR50 pKa = 11.84PLPVPKK56 pKa = 10.37DD57 pKa = 3.2FTIFRR62 pKa = 11.84KK63 pKa = 10.09AEE65 pKa = 3.78QKK67 pKa = 9.76LTILL71 pKa = 4.29

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

171

0

171

35183

38

1566

205.7

22.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.542 ± 0.227

0.924 ± 0.083

6.537 ± 0.218

6.833 ± 0.272

3.669 ± 0.116

7.148 ± 0.229

2.166 ± 0.132

5.611 ± 0.108

5.406 ± 0.247

8.22 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.606 ± 0.144

3.743 ± 0.14

4.479 ± 0.166

3.749 ± 0.167

6.401 ± 0.223

5.346 ± 0.163

5.818 ± 0.258

6.711 ± 0.194

1.356 ± 0.099

2.734 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski