Amino acid dipepetide frequency for Streptomyces sp. F-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.492AlaAla: 20.492 ± 0.194	1.14AlaCys: 1.14 ± 0.03	7.65AlaAsp: 7.65 ± 0.078	8.99AlaGlu: 8.99 ± 0.109	3.253AlaPhe: 3.253 ± 0.049	12.765AlaGly: 12.765 ± 0.11	2.964AlaHis: 2.964 ± 0.048	3.09AlaIle: 3.09 ± 0.046	2.716AlaLys: 2.716 ± 0.041	14.142AlaLeu: 14.142 ± 0.126	2.383AlaMet: 2.383 ± 0.042	1.735AlaAsn: 1.735 ± 0.03	7.265AlaPro: 7.265 ± 0.089	3.789AlaGln: 3.789 ± 0.053	11.288AlaArg: 11.288 ± 0.113	5.695AlaSer: 5.695 ± 0.057	6.154AlaThr: 6.154 ± 0.064	12.19AlaVal: 12.19 ± 0.118	1.779AlaTrp: 1.779 ± 0.033	2.589AlaTyr: 2.589 ± 0.045	0.002AlaXaa: 0.002 ± 0.001
Cys	1.217CysAla: 1.217 ± 0.029	0.117CysCys: 0.117 ± 0.009	0.541CysAsp: 0.541 ± 0.02	0.44CysGlu: 0.44 ± 0.018	0.237CysPhe: 0.237 ± 0.012	1.0CysGly: 1.0 ± 0.026	0.221CysHis: 0.221 ± 0.011	0.178CysIle: 0.178 ± 0.011	0.117CysLys: 0.117 ± 0.008	0.81CysLeu: 0.81 ± 0.023	0.133CysMet: 0.133 ± 0.009	0.124CysAsn: 0.124 ± 0.009	0.636CysPro: 0.636 ± 0.02	0.163CysGln: 0.163 ± 0.008	0.814CysArg: 0.814 ± 0.022	0.478CysSer: 0.478 ± 0.017	0.583CysThr: 0.583 ± 0.017	0.691CysVal: 0.691 ± 0.018	0.124CysTrp: 0.124 ± 0.009	0.138CysTyr: 0.138 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.131AspAla: 7.131 ± 0.081	0.428AspCys: 0.428 ± 0.018	3.476AspAsp: 3.476 ± 0.05	3.963AspGlu: 3.963 ± 0.054	1.531AspPhe: 1.531 ± 0.028	6.252AspGly: 6.252 ± 0.07	1.377AspHis: 1.377 ± 0.026	1.78AspIle: 1.78 ± 0.037	1.23AspLys: 1.23 ± 0.031	6.008AspLeu: 6.008 ± 0.066	0.809AspMet: 0.809 ± 0.021	0.898AspAsn: 0.898 ± 0.024	4.516AspPro: 4.516 ± 0.057	1.393AspGln: 1.393 ± 0.032	5.189AspArg: 5.189 ± 0.064	2.371AspSer: 2.371 ± 0.038	3.208AspThr: 3.208 ± 0.045	4.497AspVal: 4.497 ± 0.061	0.968AspTrp: 0.968 ± 0.025	1.0AspTyr: 1.0 ± 0.026	0.001AspXaa: 0.001 ± 0.001
Glu	7.64GluAla: 7.64 ± 0.09	0.384GluCys: 0.384 ± 0.015	3.081GluAsp: 3.081 ± 0.044	4.332GluGlu: 4.332 ± 0.062	1.404GluPhe: 1.404 ± 0.029	4.593GluGly: 4.593 ± 0.053	1.596GluHis: 1.596 ± 0.032	2.317GluIle: 2.317 ± 0.041	1.679GluLys: 1.679 ± 0.037	6.826GluLeu: 6.826 ± 0.068	0.948GluMet: 0.948 ± 0.024	1.169GluAsn: 1.169 ± 0.028	3.692GluPro: 3.692 ± 0.055	2.435GluGln: 2.435 ± 0.041	5.935GluArg: 5.935 ± 0.072	2.466GluSer: 2.466 ± 0.043	2.994GluThr: 2.994 ± 0.044	4.666GluVal: 4.666 ± 0.055	0.744GluTrp: 0.744 ± 0.023	1.153GluTyr: 1.153 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.388PheAla: 3.388 ± 0.045	0.287PheCys: 0.287 ± 0.014	1.8PheAsp: 1.8 ± 0.036	1.393PheGlu: 1.393 ± 0.028	0.818PhePhe: 0.818 ± 0.026	2.806PheGly: 2.806 ± 0.044	0.638PheHis: 0.638 ± 0.019	0.679PheIle: 0.679 ± 0.021	0.511PheLys: 0.511 ± 0.016	2.562PheLeu: 2.562 ± 0.043	0.379PheMet: 0.379 ± 0.015	0.518PheAsn: 0.518 ± 0.016	1.436PhePro: 1.436 ± 0.03	0.602PheGln: 0.602 ± 0.018	1.945PheArg: 1.945 ± 0.032	1.399PheSer: 1.399 ± 0.026	1.892PheThr: 1.892 ± 0.035	2.162PheVal: 2.162 ± 0.038	0.387PheTrp: 0.387 ± 0.016	0.543PheTyr: 0.543 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	10.464GlyAla: 10.464 ± 0.094	0.936GlyCys: 0.936 ± 0.026	4.952GlyAsp: 4.952 ± 0.065	5.263GlyGlu: 5.263 ± 0.06	2.743GlyPhe: 2.743 ± 0.043	8.841GlyGly: 8.841 ± 0.108	2.439GlyHis: 2.439 ± 0.043	3.367GlyIle: 3.367 ± 0.043	2.369GlyLys: 2.369 ± 0.048	9.208GlyLeu: 9.208 ± 0.076	1.943GlyMet: 1.943 ± 0.038	1.727GlyAsn: 1.727 ± 0.042	5.654GlyPro: 5.654 ± 0.074	2.528GlyGln: 2.528 ± 0.042	8.933GlyArg: 8.933 ± 0.09	5.095GlySer: 5.095 ± 0.066	6.641GlyThr: 6.641 ± 0.066	7.313GlyVal: 7.313 ± 0.077	1.584GlyTrp: 1.584 ± 0.03	2.057GlyTyr: 2.057 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.71HisAla: 2.71 ± 0.036	0.232HisCys: 0.232 ± 0.012	1.363HisAsp: 1.363 ± 0.028	1.295HisGlu: 1.295 ± 0.028	0.645HisPhe: 0.645 ± 0.02	2.629HisGly: 2.629 ± 0.04	0.713HisHis: 0.713 ± 0.021	0.698HisIle: 0.698 ± 0.024	0.4HisLys: 0.4 ± 0.016	2.46HisLeu: 2.46 ± 0.039	0.345HisMet: 0.345 ± 0.015	0.364HisAsn: 0.364 ± 0.015	1.933HisPro: 1.933 ± 0.038	0.622HisGln: 0.622 ± 0.021	2.448HisArg: 2.448 ± 0.039	0.98HisSer: 0.98 ± 0.025	1.342HisThr: 1.342 ± 0.033	1.687HisVal: 1.687 ± 0.036	0.377HisTrp: 0.377 ± 0.013	0.461HisTyr: 0.461 ± 0.015	0.001HisXaa: 0.001 ± 0.001
Ile	4.518IleAla: 4.518 ± 0.055	0.303IleCys: 0.303 ± 0.012	2.17IleAsp: 2.17 ± 0.031	1.992IleGlu: 1.992 ± 0.039	0.652IlePhe: 0.652 ± 0.019	3.414IleGly: 3.414 ± 0.054	0.607IleHis: 0.607 ± 0.02	0.821IleIle: 0.821 ± 0.026	0.743IleLys: 0.743 ± 0.021	2.275IleLeu: 2.275 ± 0.041	0.424IleMet: 0.424 ± 0.016	0.678IleAsn: 0.678 ± 0.023	1.805IlePro: 1.805 ± 0.032	0.696IleGln: 0.696 ± 0.02	2.476IleArg: 2.476 ± 0.04	1.502IleSer: 1.502 ± 0.032	2.117IleThr: 2.117 ± 0.039	2.673IleVal: 2.673 ± 0.046	0.349IleTrp: 0.349 ± 0.016	0.484IleTyr: 0.484 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	3.032LysAla: 3.032 ± 0.053	0.129LysCys: 0.129 ± 0.009	1.429LysAsp: 1.429 ± 0.033	1.451LysGlu: 1.451 ± 0.034	0.421LysPhe: 0.421 ± 0.014	1.979LysGly: 1.979 ± 0.046	0.43LysHis: 0.43 ± 0.014	0.838LysIle: 0.838 ± 0.025	0.907LysLys: 0.907 ± 0.038	1.952LysLeu: 1.952 ± 0.034	0.385LysMet: 0.385 ± 0.016	0.557LysAsn: 0.557 ± 0.021	1.387LysPro: 1.387 ± 0.034	0.747LysGln: 0.747 ± 0.023	1.569LysArg: 1.569 ± 0.033	1.098LysSer: 1.098 ± 0.026	1.233LysThr: 1.233 ± 0.034	1.906LysVal: 1.906 ± 0.039	0.26LysTrp: 0.26 ± 0.013	0.472LysTyr: 0.472 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.392LeuAla: 14.392 ± 0.131	0.926LeuCys: 0.926 ± 0.023	6.407LeuAsp: 6.407 ± 0.066	5.253LeuGlu: 5.253 ± 0.063	2.5LeuPhe: 2.5 ± 0.042	8.927LeuGly: 8.927 ± 0.088	2.361LeuHis: 2.361 ± 0.044	3.173LeuIle: 3.173 ± 0.043	2.141LeuLys: 2.141 ± 0.041	11.394LeuLeu: 11.394 ± 0.113	1.657LeuMet: 1.657 ± 0.033	1.583LeuAsn: 1.583 ± 0.029	6.64LeuPro: 6.64 ± 0.075	2.189LeuGln: 2.189 ± 0.042	9.376LeuArg: 9.376 ± 0.091	5.101LeuSer: 5.101 ± 0.057	6.598LeuThr: 6.598 ± 0.069	8.62LeuVal: 8.62 ± 0.081	1.288LeuTrp: 1.288 ± 0.025	1.834LeuTyr: 1.834 ± 0.033	0.001LeuXaa: 0.001 ± 0.001
Met	2.246MetAla: 2.246 ± 0.039	0.153MetCys: 0.153 ± 0.008	0.888MetAsp: 0.888 ± 0.022	0.805MetGlu: 0.805 ± 0.021	0.447MetPhe: 0.447 ± 0.017	1.334MetGly: 1.334 ± 0.029	0.367MetHis: 0.367 ± 0.015	0.682MetIle: 0.682 ± 0.019	0.406MetLys: 0.406 ± 0.015	1.673MetLeu: 1.673 ± 0.035	0.294MetMet: 0.294 ± 0.015	0.418MetAsn: 0.418 ± 0.015	1.167MetPro: 1.167 ± 0.026	0.438MetGln: 0.438 ± 0.017	1.57MetArg: 1.57 ± 0.032	1.268MetSer: 1.268 ± 0.025	1.466MetThr: 1.466 ± 0.029	1.246MetVal: 1.246 ± 0.027	0.201MetTrp: 0.201 ± 0.011	0.325MetTyr: 0.325 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.104AsnAla: 2.104 ± 0.04	0.153AsnCys: 0.153 ± 0.009	0.894AsnAsp: 0.894 ± 0.023	0.809AsnGlu: 0.809 ± 0.021	0.443AsnPhe: 0.443 ± 0.017	1.817AsnGly: 1.817 ± 0.039	0.388AsnHis: 0.388 ± 0.014	0.613AsnIle: 0.613 ± 0.02	0.452AsnLys: 0.452 ± 0.017	1.587AsnLeu: 1.587 ± 0.033	0.28AsnMet: 0.28 ± 0.014	0.405AsnAsn: 0.405 ± 0.016	1.334AsnPro: 1.334 ± 0.028	0.477AsnGln: 0.477 ± 0.018	1.382AsnArg: 1.382 ± 0.028	0.853AsnSer: 0.853 ± 0.023	1.032AsnThr: 1.032 ± 0.028	1.315AsnVal: 1.315 ± 0.032	0.258AsnTrp: 0.258 ± 0.013	0.367AsnTyr: 0.367 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.943ProAla: 8.943 ± 0.104	0.484ProCys: 0.484 ± 0.019	4.608ProAsp: 4.608 ± 0.058	4.648ProGlu: 4.648 ± 0.053	1.555ProPhe: 1.555 ± 0.034	7.082ProGly: 7.082 ± 0.075	1.534ProHis: 1.534 ± 0.03	1.219ProIle: 1.219 ± 0.027	1.239ProLys: 1.239 ± 0.036	5.584ProLeu: 5.584 ± 0.057	1.045ProMet: 1.045 ± 0.026	0.837ProAsn: 0.837 ± 0.023	4.168ProPro: 4.168 ± 0.079	1.777ProGln: 1.777 ± 0.035	4.83ProArg: 4.83 ± 0.06	3.486ProSer: 3.486 ± 0.056	2.907ProThr: 2.907 ± 0.044	5.847ProVal: 5.847 ± 0.068	0.935ProTrp: 0.935 ± 0.023	1.476ProTyr: 1.476 ± 0.033	0.001ProXaa: 0.001 ± 0.001
Gln	3.689GlnAla: 3.689 ± 0.053	0.186GlnCys: 0.186 ± 0.01	1.37GlnAsp: 1.37 ± 0.03	1.677GlnGlu: 1.677 ± 0.035	0.623GlnPhe: 0.623 ± 0.019	2.311GlnGly: 2.311 ± 0.039	0.61GlnHis: 0.61 ± 0.017	1.036GlnIle: 1.036 ± 0.026	0.714GlnLys: 0.714 ± 0.02	2.845GlnLeu: 2.845 ± 0.039	0.507GlnMet: 0.507 ± 0.016	0.48GlnAsn: 0.48 ± 0.018	1.681GlnPro: 1.681 ± 0.041	1.215GlnGln: 1.215 ± 0.033	2.453GlnArg: 2.453 ± 0.04	1.204GlnSer: 1.204 ± 0.029	1.274GlnThr: 1.274 ± 0.028	2.251GlnVal: 2.251 ± 0.044	0.437GlnTrp: 0.437 ± 0.015	0.584GlnTyr: 0.584 ± 0.022	0.001GlnXaa: 0.001 ± 0.001
Arg	10.75ArgAla: 10.75 ± 0.112	0.757ArgCys: 0.757 ± 0.022	4.341ArgAsp: 4.341 ± 0.061	5.259ArgGlu: 5.259 ± 0.071	2.428ArgPhe: 2.428 ± 0.039	6.544ArgGly: 6.544 ± 0.075	2.402ArgHis: 2.402 ± 0.038	3.627ArgIle: 3.627 ± 0.048	1.963ArgLys: 1.963 ± 0.037	9.668ArgLeu: 9.668 ± 0.09	1.952ArgMet: 1.952 ± 0.039	1.49ArgAsn: 1.49 ± 0.033	5.951ArgPro: 5.951 ± 0.077	2.487ArgGln: 2.487 ± 0.035	9.374ArgArg: 9.374 ± 0.113	4.396ArgSer: 4.396 ± 0.054	6.205ArgThr: 6.205 ± 0.068	6.154ArgVal: 6.154 ± 0.069	1.411ArgTrp: 1.411 ± 0.033	1.83ArgTyr: 1.83 ± 0.036	0.001ArgXaa: 0.001 ± 0.001
Ser	6.418SerAla: 6.418 ± 0.061	0.476SerCys: 0.476 ± 0.017	2.531SerAsp: 2.531 ± 0.039	2.395SerGlu: 2.395 ± 0.042	1.509SerPhe: 1.509 ± 0.028	5.674SerGly: 5.674 ± 0.06	0.981SerHis: 0.981 ± 0.024	1.272SerIle: 1.272 ± 0.029	0.965SerLys: 0.965 ± 0.024	4.648SerLeu: 4.648 ± 0.048	1.078SerMet: 1.078 ± 0.027	0.765SerAsn: 0.765 ± 0.019	3.367SerPro: 3.367 ± 0.048	1.149SerGln: 1.149 ± 0.025	4.047SerArg: 4.047 ± 0.047	2.897SerSer: 2.897 ± 0.046	2.859SerThr: 2.859 ± 0.046	3.957SerVal: 3.957 ± 0.052	0.853SerTrp: 0.853 ± 0.022	1.132SerTyr: 1.132 ± 0.029	0.001SerXaa: 0.001 ± 0.001
Thr	8.722ThrAla: 8.722 ± 0.081	0.483ThrCys: 0.483 ± 0.018	3.451ThrAsp: 3.451 ± 0.049	3.241ThrGlu: 3.241 ± 0.046	1.522ThrPhe: 1.522 ± 0.03	6.736ThrGly: 6.736 ± 0.066	1.209ThrHis: 1.209 ± 0.028	1.612ThrIle: 1.612 ± 0.036	1.102ThrLys: 1.102 ± 0.029	5.425ThrLeu: 5.425 ± 0.058	0.923ThrMet: 0.923 ± 0.021	0.914ThrAsn: 0.914 ± 0.025	4.158ThrPro: 4.158 ± 0.051	1.229ThrGln: 1.229 ± 0.029	4.247ThrArg: 4.247 ± 0.059	2.946ThrSer: 2.946 ± 0.045	3.641ThrThr: 3.641 ± 0.068	6.01ThrVal: 6.01 ± 0.068	0.862ThrTrp: 0.862 ± 0.026	1.296ThrTyr: 1.296 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	10.047ValAla: 10.047 ± 0.101	0.835ValCys: 0.835 ± 0.024	4.78ValAsp: 4.78 ± 0.055	4.824ValGlu: 4.824 ± 0.053	2.322ValPhe: 2.322 ± 0.043	6.117ValGly: 6.117 ± 0.071	2.121ValHis: 2.121 ± 0.035	2.664ValIle: 2.664 ± 0.041	1.725ValLys: 1.725 ± 0.035	9.654ValLeu: 9.654 ± 0.094	1.38ValMet: 1.38 ± 0.032	1.51ValAsn: 1.51 ± 0.03	5.625ValPro: 5.625 ± 0.06	2.063ValGln: 2.063 ± 0.034	7.9ValArg: 7.9 ± 0.079	4.024ValSer: 4.024 ± 0.047	5.446ValThr: 5.446 ± 0.06	8.199ValVal: 8.199 ± 0.096	1.119ValTrp: 1.119 ± 0.023	1.539ValTyr: 1.539 ± 0.031	0.001ValXaa: 0.001 ± 0.001
Trp	1.564TrpAla: 1.564 ± 0.031	0.169TrpCys: 0.169 ± 0.01	0.753TrpAsp: 0.753 ± 0.019	0.726TrpGlu: 0.726 ± 0.022	0.484TrpPhe: 0.484 ± 0.017	1.012TrpGly: 1.012 ± 0.023	0.362TrpHis: 0.362 ± 0.015	0.531TrpIle: 0.531 ± 0.017	0.372TrpLys: 0.372 ± 0.018	1.697TrpLeu: 1.697 ± 0.037	0.287TrpMet: 0.287 ± 0.013	0.411TrpAsn: 0.411 ± 0.017	0.785TrpPro: 0.785 ± 0.025	0.578TrpGln: 0.578 ± 0.018	1.35TrpArg: 1.35 ± 0.028	0.85TrpSer: 0.85 ± 0.023	1.024TrpThr: 1.024 ± 0.026	0.948TrpVal: 0.948 ± 0.024	0.335TrpTrp: 0.335 ± 0.015	0.355TrpTyr: 0.355 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.603TyrAla: 2.603 ± 0.04	0.185TyrCys: 0.185 ± 0.01	1.539TyrAsp: 1.539 ± 0.037	1.297TyrGlu: 1.297 ± 0.027	0.61TyrPhe: 0.61 ± 0.018	2.224TyrGly: 2.224 ± 0.046	0.387TyrHis: 0.387 ± 0.015	0.503TyrIle: 0.503 ± 0.018	0.427TyrLys: 0.427 ± 0.015	1.942TyrLeu: 1.942 ± 0.037	0.242TyrMet: 0.242 ± 0.011	0.379TyrAsn: 0.379 ± 0.018	1.0TyrPro: 1.0 ± 0.024	0.57TyrGln: 0.57 ± 0.019	1.864TyrArg: 1.864 ± 0.033	0.862TyrSer: 0.862 ± 0.022	1.113TyrThr: 1.113 ± 0.033	1.561TyrVal: 1.561 ± 0.03	0.325TyrTrp: 0.325 ± 0.014	0.414TyrTyr: 0.414 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 6040 proteins (1761871 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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