Anomala cuprea entomopoxvirus
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 241 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W6JLL8|W6JLL8_9POXV PPM-type phosphatase domain-containing protein OS=Anomala cuprea entomopoxvirus OX=62099 PE=4 SV=1
MM1 pKa = 7.26 YY2 pKa = 9.41 LTMKK6 pKa = 10.29 DD7 pKa = 3.86 LNSILYY13 pKa = 9.91 LNNDD17 pKa = 4.13 IIDD20 pKa = 3.76 EE21 pKa = 4.3 THH23 pKa = 6.75 YY24 pKa = 10.16 DD25 pKa = 3.78 TINISILDD33 pKa = 3.93 ILPEE37 pKa = 4.66 FIDD40 pKa = 4.64 LIKK43 pKa = 11.23 NSVDD47 pKa = 3.26 NNLL50 pKa = 3.56
Molecular weight: 5.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.795
IPC_protein 3.694
Toseland 3.49
ProMoST 3.923
Dawson 3.719
Bjellqvist 3.897
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.834
Patrickios 1.901
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|W6JPM5|W6JPM5_9POXV Nucleopolyhedrovirus p26 protein OS=Anomala cuprea entomopoxvirus OX=62099 PE=4 SV=1
MM1 pKa = 8.0 DD2 pKa = 3.79 VPIINLDD9 pKa = 3.06 NLYY12 pKa = 9.9 IHH14 pKa = 6.87 NGNKK18 pKa = 9.46 SRR20 pKa = 11.84 SFKK23 pKa = 10.71 QLLNNLQAKK32 pKa = 7.66 YY33 pKa = 10.84 LKK35 pKa = 10.35 DD36 pKa = 3.43 QEE38 pKa = 4.23 KK39 pKa = 10.12 TIDD42 pKa = 3.62 YY43 pKa = 10.7 LNTYY47 pKa = 10.85 DD48 pKa = 5.55 NIAGKK53 pKa = 9.85 NIKK56 pKa = 9.46 KK57 pKa = 10.5 KK58 pKa = 10.62 NGSTKK63 pKa = 9.49 VKK65 pKa = 10.19 EE66 pKa = 4.37 GAKK69 pKa = 9.99 HH70 pKa = 5.25 EE71 pKa = 4.07 LVGIYY76 pKa = 10.11 KK77 pKa = 9.88 DD78 pKa = 4.04 APPIHH83 pKa = 6.99 CIVCKK88 pKa = 10.52 SVTKK92 pKa = 10.83 NIDD95 pKa = 3.1 PVQVYY100 pKa = 7.87 FSQNKK105 pKa = 7.88 TRR107 pKa = 11.84 LKK109 pKa = 9.2 CTCINCNNSKK119 pKa = 10.34 SSYY122 pKa = 10.16 IRR124 pKa = 11.84 FEE126 pKa = 4.02 KK127 pKa = 10.56 LPSYY131 pKa = 9.8 LQQKK135 pKa = 9.42 LPPNLQRR142 pKa = 11.84 KK143 pKa = 8.54 DD144 pKa = 3.25 AA145 pKa = 4.45
Molecular weight: 16.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.099
IPC2_protein 8.96
IPC_protein 8.829
Toseland 9.648
ProMoST 9.341
Dawson 9.882
Bjellqvist 9.589
Wikipedia 10.028
Rodwell 10.365
Grimsley 9.94
Solomon 9.911
Lehninger 9.882
Nozaki 9.765
DTASelect 9.531
Thurlkill 9.736
EMBOSS 10.072
Sillero 9.823
Patrickios 7.556
IPC_peptide 9.911
IPC2_peptide 8.229
IPC2.peptide.svr19 8.024
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
241
0
241
67363
50
1506
279.5
33.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.869 ± 0.073
1.958 ± 0.153
6.115 ± 0.109
4.893 ± 0.127
4.836 ± 0.097
2.553 ± 0.124
1.789 ± 0.079
14.722 ± 0.235
9.057 ± 0.173
9.284 ± 0.155
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.848 ± 0.057
11.949 ± 0.223
2.167 ± 0.081
2.111 ± 0.072
2.421 ± 0.081
6.141 ± 0.122
5.067 ± 0.105
3.22 ± 0.1
0.548 ± 0.041
7.452 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here