Candidatus Micrarchaeum sp. AZ1

Taxonomy: cellular organisms; Archaea; DPANN group; Candidatus Micrarchaeota; Candidatus Micrarchaeum; unclassified Candidatus Micrarchaeum

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 840 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M2YIG1|A0A1M2YIG1_9ARCH Uncharacterized protein OS=Candidatus Micrarchaeum sp. AZ1 OX=1531428 GN=JJ59_01150 PE=4 SV=1
AA1 pKa = 7.36VVPIFFNISNIPTSLQSNPSGIATIDD27 pKa = 3.68GVSYY31 pKa = 10.65SYY33 pKa = 11.38SQLTKK38 pKa = 10.69KK39 pKa = 8.41PTNFDD44 pKa = 3.22WGCNTTHH51 pKa = 7.21TYY53 pKa = 10.78SFDD56 pKa = 4.43PIIYY60 pKa = 9.0NSSGTRR66 pKa = 11.84FVFKK70 pKa = 10.63SVTIDD75 pKa = 3.46GFTSDD80 pKa = 4.5SNSGSATVSTCKK92 pKa = 10.31SQNVTVSYY100 pKa = 10.97AEE102 pKa = 3.87QYY104 pKa = 10.13EE105 pKa = 4.56LYY107 pKa = 10.4DD108 pKa = 3.37IASPSIGGTVSPATSWYY125 pKa = 9.8APSSSVTISEE135 pKa = 4.48TPNTTGHH142 pKa = 5.92YY143 pKa = 10.81VFIDD147 pKa = 3.57WTCTGTGCYY156 pKa = 9.95SGTATSTSVTLNNPINEE173 pKa = 4.27TAVFQSTTTSTSTTSTSTTTSTTSTSTTTSTTSTSTSTTSTSTTTSTSTTTSTSTTSTSTTSTSTTTSTTSTSTSTTSTSTTTSTSTTTSTSTTTSTSTTTSTSTTTSSSTIPFYY288 pKa = 10.0GTCTTNSSSATTFSPSCSNNNGVFSEE314 pKa = 4.71CVAAIYY320 pKa = 10.8QSMSSVDD327 pKa = 3.17WNINSEE333 pKa = 4.19GGGTCQYY340 pKa = 10.72CSTNIGYY347 pKa = 7.61ITNGNACSATADD359 pKa = 3.83SSSSGAIAIGGNPATNYY376 pKa = 9.51QLWATNGSNYY386 pKa = 10.04NSGVQPVFWVNHH398 pKa = 4.42GSIPSSLVILTYY410 pKa = 9.59ATSGGQEE417 pKa = 4.43GILNSTTEE425 pKa = 4.38PGTSAIMYY433 pKa = 9.36DD434 pKa = 3.47GGSFPTYY441 pKa = 9.41GCSVTEE447 pKa = 4.15NLEE450 pKa = 3.74QTTPLFSSTHH460 pKa = 5.44YY461 pKa = 10.51NDD463 pKa = 4.16VYY465 pKa = 11.09ILACGNIPYY474 pKa = 10.02DD475 pKa = 3.99GTGGWIGPEE484 pKa = 4.03LMTGSSTSFSYY495 pKa = 10.69AVYY498 pKa = 10.6VFNPDD503 pKa = 2.48STYY506 pKa = 11.12TFNKK510 pKa = 10.15NSS512 pKa = 3.35

Molecular weight: 52.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M2YH48|A0A1M2YH48_9ARCH Uncharacterized protein OS=Candidatus Micrarchaeum sp. AZ1 OX=1531428 GN=JJ59_02780 PE=4 SV=1
MM1 pKa = 7.66PRR3 pKa = 11.84QSPKK7 pKa = 10.4TKK9 pKa = 10.49DD10 pKa = 3.11LARR13 pKa = 11.84AIARR17 pKa = 11.84KK18 pKa = 9.18RR19 pKa = 11.84VAGLISLSEE28 pKa = 3.87KK29 pKa = 10.62ALRR32 pKa = 11.84DD33 pKa = 3.59GNQEE37 pKa = 3.47LSRR40 pKa = 11.84RR41 pKa = 11.84YY42 pKa = 10.2AEE44 pKa = 4.27LASKK48 pKa = 9.71IQSHH52 pKa = 4.71YY53 pKa = 10.43RR54 pKa = 11.84IRR56 pKa = 11.84PSLRR60 pKa = 11.84YY61 pKa = 8.82RR62 pKa = 11.84VCKK65 pKa = 10.18NCGSILVPGITSSTRR80 pKa = 11.84LSSSRR85 pKa = 11.84GYY87 pKa = 10.18IVVKK91 pKa = 10.51CMVCGTEE98 pKa = 3.63LHH100 pKa = 6.15KK101 pKa = 10.92VYY103 pKa = 10.86KK104 pKa = 10.06KK105 pKa = 10.89

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

840

0

840

239489

50

1152

285.1

31.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.082 ± 0.086

0.703 ± 0.026

4.599 ± 0.069

6.417 ± 0.121

4.292 ± 0.074

7.076 ± 0.072

1.512 ± 0.03

8.288 ± 0.074

7.801 ± 0.12

8.845 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.016 ± 0.039

4.752 ± 0.085

3.497 ± 0.052

2.365 ± 0.053

4.248 ± 0.078

8.268 ± 0.115

4.831 ± 0.079

6.439 ± 0.069

0.709 ± 0.024

4.261 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski