Andrena haemorrhoa nege-like virus
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L2P6U6|A0A2L2P6U6_9VIRU ORF1 OS=Andrena haemorrhoa nege-like virus OX=2094259 PE=4 SV=1
MM1 pKa = 7.29 SPLRR5 pKa = 11.84 VFVLSYY11 pKa = 10.62 LVSFGLSEE19 pKa = 4.09 FTTLLGNLPDD29 pKa = 3.94 EE30 pKa = 4.49 VLEE33 pKa = 4.19 QIQVTEE39 pKa = 3.74 DD40 pKa = 3.15 RR41 pKa = 11.84 YY42 pKa = 9.86 RR43 pKa = 11.84 TFLDD47 pKa = 3.94 YY48 pKa = 11.55 YY49 pKa = 10.42 DD50 pKa = 3.92 TADD53 pKa = 3.25 VKK55 pKa = 11.19 AYY57 pKa = 10.27 DD58 pKa = 4.44 LSWASKK64 pKa = 9.22 FFKK67 pKa = 10.83 DD68 pKa = 4.09 PVCHH72 pKa = 6.34 NGWVLHH78 pKa = 6.01 LLSNSAFRR86 pKa = 11.84 DD87 pKa = 3.67 EE88 pKa = 5.22 YY89 pKa = 8.99 YY90 pKa = 10.9 LCVRR94 pKa = 11.84 TEE96 pKa = 4.16 GNKK99 pKa = 9.54 KK100 pKa = 10.14 RR101 pKa = 11.84 LIEE104 pKa = 4.08 TTQLRR109 pKa = 11.84 FSDD112 pKa = 3.51 QQQWIFSDD120 pKa = 3.35 ADD122 pKa = 3.52 NQVGVIVLEE131 pKa = 3.7 LDD133 pKa = 3.3 IPNKK137 pKa = 10.47 YY138 pKa = 9.95 IFYY141 pKa = 10.53 KK142 pKa = 10.94 DD143 pKa = 3.59 GDD145 pKa = 4.47 DD146 pKa = 5.61 LYY148 pKa = 8.68 MTTPMCMSNHH158 pKa = 6.31 YY159 pKa = 10.38 YY160 pKa = 9.51 LTDD163 pKa = 3.85 DD164 pKa = 4.9 KK165 pKa = 10.82 IFPPEE170 pKa = 3.74 PPIEE174 pKa = 4.15 NKK176 pKa = 10.39 KK177 pKa = 10.51 NFIKK181 pKa = 10.79 YY182 pKa = 9.84 FYY184 pKa = 9.35 TYY186 pKa = 7.76 QCRR189 pKa = 11.84 PVRR192 pKa = 11.84 VHH194 pKa = 6.08 NTLKK198 pKa = 10.34 QLPIEE203 pKa = 4.53 VKK205 pKa = 8.77 WRR207 pKa = 11.84 VAGGQGYY214 pKa = 9.14 SPEE217 pKa = 3.91 YY218 pKa = 10.86 VVFLEE223 pKa = 5.62 DD224 pKa = 3.43 AFKK227 pKa = 11.45 SNLLRR232 pKa = 11.84 QLDD235 pKa = 3.45 QDD237 pKa = 3.87 AFVPTDD243 pKa = 3.41 NLYY246 pKa = 10.85 YY247 pKa = 10.8 SPDD250 pKa = 3.61 YY251 pKa = 10.96 NVTIMDD257 pKa = 4.1 TPSVYY262 pKa = 10.2 AVKK265 pKa = 10.54 YY266 pKa = 10.4 LNHH269 pKa = 6.58 LPDD272 pKa = 3.28 YY273 pKa = 9.91 EE274 pKa = 5.15 IIYY277 pKa = 10.12 FEE279 pKa = 5.68 HH280 pKa = 5.95 ITSNFFLDD288 pKa = 4.87 LIEE291 pKa = 4.6 FVLDD295 pKa = 3.58 RR296 pKa = 11.84 VISLIVTILSTIFNFVLEE314 pKa = 5.08 KK315 pKa = 10.23 INQYY319 pKa = 9.89 VFSNIPYY326 pKa = 8.12 YY327 pKa = 10.66 FKK329 pKa = 10.9 SFYY332 pKa = 11.0 EE333 pKa = 4.45 FFFSLDD339 pKa = 3.55 PYY341 pKa = 11.28 VKK343 pKa = 9.92 FLIMSFVLIYY353 pKa = 10.55 IKK355 pKa = 7.44 TTKK358 pKa = 10.25 FIKK361 pKa = 10.01 TIFLVFLLTLFTYY374 pKa = 10.89 NKK376 pKa = 9.01 LTT378 pKa = 3.52
Molecular weight: 44.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.765
IPC2_protein 5.003
IPC_protein 4.952
Toseland 4.863
ProMoST 5.105
Dawson 4.952
Bjellqvist 5.08
Wikipedia 4.851
Rodwell 4.851
Grimsley 4.774
Solomon 4.952
Lehninger 4.914
Nozaki 5.067
DTASelect 5.27
Thurlkill 4.876
EMBOSS 4.889
Sillero 5.13
Patrickios 2.079
IPC_peptide 4.952
IPC2_peptide 5.118
IPC2.peptide.svr19 5.049
Protein with the highest isoelectric point:
>tr|A0A2L2P6U8|A0A2L2P6U8_9VIRU ORF3 OS=Andrena haemorrhoa nege-like virus OX=2094259 PE=4 SV=1
MM1 pKa = 7.24 SQRR4 pKa = 11.84 KK5 pKa = 7.17 QAISSAVRR13 pKa = 11.84 RR14 pKa = 11.84 TTVQPVVVKK23 pKa = 9.94 PKK25 pKa = 10.59 GRR27 pKa = 11.84 LTSVRR32 pKa = 11.84 NKK34 pKa = 9.63 FRR36 pKa = 11.84 NSVKK40 pKa = 10.79 SFDD43 pKa = 3.41 LTSFYY48 pKa = 11.02 NYY50 pKa = 9.01 TLNVVSDD57 pKa = 3.73 PTFLLLYY64 pKa = 9.48 CLSIYY69 pKa = 10.16 ICYY72 pKa = 10.05 DD73 pKa = 3.09 YY74 pKa = 11.65 SNSHH78 pKa = 6.93 DD79 pKa = 3.54 KK80 pKa = 11.21 SHH82 pKa = 6.72 IFKK85 pKa = 9.04 FTKK88 pKa = 10.53 NLVTTFPSLSASACAVYY105 pKa = 10.52 NWIVAFIPFLPVILTVPPKK124 pKa = 10.62 LRR126 pKa = 11.84 IPTFIGTILYY136 pKa = 10.57 YY137 pKa = 10.8 SFIPEE142 pKa = 3.95 RR143 pKa = 11.84 TVYY146 pKa = 10.15 EE147 pKa = 4.0 YY148 pKa = 10.68 LIHH151 pKa = 6.5 SVCIYY156 pKa = 10.61 LFIRR160 pKa = 11.84 TRR162 pKa = 11.84 NRR164 pKa = 11.84 YY165 pKa = 8.64 FRR167 pKa = 11.84 ILSLSLLFLSYY178 pKa = 11.23 VMQFAIPLPANSVLVCNSTTFKK200 pKa = 11.17
Molecular weight: 23.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.645
IPC2_protein 9.663
IPC_protein 9.882
Toseland 9.765
ProMoST 9.736
Dawson 10.101
Bjellqvist 9.897
Wikipedia 10.35
Rodwell 10.292
Grimsley 10.218
Solomon 10.131
Lehninger 10.072
Nozaki 9.867
DTASelect 9.867
Thurlkill 9.926
EMBOSS 10.218
Sillero 10.043
Patrickios 4.329
IPC_peptide 10.116
IPC2_peptide 8.946
IPC2.peptide.svr19 8.4
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2822
200
2244
940.7
109.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.827 ± 0.443
2.091 ± 0.303
5.634 ± 0.894
5.315 ± 1.221
6.697 ± 0.919
2.339 ± 0.349
1.949 ± 0.194
7.583 ± 0.208
6.804 ± 0.663
10.241 ± 0.475
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.447
7.335 ± 1.126
3.756 ± 0.775
2.835 ± 0.23
3.863 ± 0.522
8.186 ± 0.829
6.201 ± 0.54
6.839 ± 0.898
0.39 ± 0.225
5.882 ± 0.892
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here