Sida mottle Alagoas virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7YFK9|L7YFK9_9GEMI Capsid protein OS=Sida mottle Alagoas virus OX=1266995 PE=3 SV=1
MM1 pKa = 7.28PRR3 pKa = 11.84KK4 pKa = 10.11GSFCIKK10 pKa = 9.86AKK12 pKa = 10.7NYY14 pKa = 9.52FLTFPRR20 pKa = 11.84CSLTKK25 pKa = 10.64EE26 pKa = 3.97EE27 pKa = 4.88ALSQILNLKK36 pKa = 8.6TPVNKK41 pKa = 10.17KK42 pKa = 9.76FIKK45 pKa = 9.73ICRR48 pKa = 11.84EE49 pKa = 3.68LHH51 pKa = 7.45DD52 pKa = 5.41DD53 pKa = 4.39GEE55 pKa = 4.48PHH57 pKa = 6.89LHH59 pKa = 6.41VLLQFEE65 pKa = 5.61GKK67 pKa = 9.17FQCTNNRR74 pKa = 11.84FFDD77 pKa = 3.78MVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.04VKK101 pKa = 11.23SYY103 pKa = 10.86VEE105 pKa = 3.96KK106 pKa = 11.17DD107 pKa = 3.09GDD109 pKa = 3.75TMEE112 pKa = 4.12WGEE115 pKa = 3.96FQVDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQTSNDD132 pKa = 3.69AAAEE136 pKa = 3.98ALNAATKK143 pKa = 9.82EE144 pKa = 3.82AALQIIKK151 pKa = 10.44EE152 pKa = 4.38KK153 pKa = 10.65IPEE156 pKa = 4.11KK157 pKa = 10.92YY158 pKa = 10.34LFQFHH163 pKa = 6.7NLNSNLDD170 pKa = 4.13RR171 pKa = 11.84IFSKK175 pKa = 10.83APEE178 pKa = 4.03PWAPPSLLCSFTNVPDD194 pKa = 4.63EE195 pKa = 4.14MQEE198 pKa = 3.79WADD201 pKa = 3.78DD202 pKa = 3.86YY203 pKa = 11.3FGRR206 pKa = 11.84GAAARR211 pKa = 11.84PEE213 pKa = 4.13RR214 pKa = 11.84PISLIVEE221 pKa = 4.55GDD223 pKa = 3.35SRR225 pKa = 11.84TGKK228 pKa = 8.55TMWARR233 pKa = 11.84ALGPHH238 pKa = 6.63NYY240 pKa = 10.14LSGHH244 pKa = 6.93LDD246 pKa = 3.73FNPRR250 pKa = 11.84IYY252 pKa = 10.96SDD254 pKa = 3.41DD255 pKa = 3.64AEE257 pKa = 4.58YY258 pKa = 11.35NVIDD262 pKa = 4.99DD263 pKa = 4.11VTPHH267 pKa = 5.44YY268 pKa = 10.89LKK270 pKa = 10.69LKK272 pKa = 9.09HH273 pKa = 5.7WKK275 pKa = 9.67EE276 pKa = 3.98LIGAQKK282 pKa = 10.57DD283 pKa = 3.42WQSNCKK289 pKa = 9.38YY290 pKa = 10.21GKK292 pKa = 9.18PVQIKK297 pKa = 10.19GGIPSIVLCNPGEE310 pKa = 4.16GASYY314 pKa = 11.29KK315 pKa = 10.87DD316 pKa = 3.72FLDD319 pKa = 3.95KK320 pKa = 11.34EE321 pKa = 4.56EE322 pKa = 4.13NASLKK327 pKa = 10.71AWTLHH332 pKa = 4.56NAKK335 pKa = 10.34FIFLNSPLYY344 pKa = 9.3QTTTQDD350 pKa = 3.63CQEE353 pKa = 4.51EE354 pKa = 4.62SSQATTSS361 pKa = 3.14

Molecular weight: 40.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7YDB5|L7YDB5_9GEMI Replication enhancer OS=Sida mottle Alagoas virus OX=1266995 PE=3 SV=1
MM1 pKa = 7.62GSLISTSSSSSKK13 pKa = 10.63GNSNARR19 pKa = 11.84ITDD22 pKa = 3.3SSTWCPQQGQHH33 pKa = 5.39ISIRR37 pKa = 11.84TFRR40 pKa = 11.84EE41 pKa = 3.69LNHH44 pKa = 6.87RR45 pKa = 11.84PTSSPTSRR53 pKa = 11.84KK54 pKa = 8.13TEE56 pKa = 4.13TPWNGEE62 pKa = 3.89NSRR65 pKa = 11.84STAEE69 pKa = 3.97VLEE72 pKa = 4.52EE73 pKa = 3.81VAKK76 pKa = 10.77RR77 pKa = 11.84LTTLQQRR84 pKa = 11.84HH85 pKa = 5.11

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

958

85

361

191.6

21.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.637 ± 1.156

1.983 ± 0.391

5.115 ± 0.675

4.906 ± 0.91

4.28 ± 0.658

5.741 ± 0.464

3.653 ± 0.482

5.219 ± 0.39

6.263 ± 0.997

6.889 ± 0.853

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.296 ± 0.644

6.159 ± 0.435

5.532 ± 0.619

4.28 ± 0.483

6.889 ± 1.326

8.246 ± 1.469

6.054 ± 0.929

5.219 ± 1.118

1.879 ± 0.084

3.758 ± 0.819
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski