Streptococcus phage P0091

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286QNI6|A0A286QNI6_9CAUD Major capsid protein OS=Streptococcus phage P0091 OX=1971410 GN=P0091_06 PE=4 SV=1
MM1 pKa = 7.3SVSKK5 pKa = 9.08EE6 pKa = 4.26TIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.69IEE28 pKa = 4.01SAKK31 pKa = 10.51QYY33 pKa = 10.21IINAVGSDD41 pKa = 3.14QKK43 pKa = 11.06FYY45 pKa = 11.02EE46 pKa = 4.29LEE48 pKa = 3.93NVKK51 pKa = 10.63YY52 pKa = 10.77LFDD55 pKa = 3.62TAVIALTSSYY65 pKa = 8.66FTYY68 pKa = 10.07RR69 pKa = 11.84VALTDD74 pKa = 3.49TVTYY78 pKa = 9.35PINLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.4ATYY98 pKa = 10.89SEE100 pKa = 4.8VVANGG105 pKa = 3.05

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286QNI5|A0A286QNI5_9CAUD DNA packaging protein OS=Streptococcus phage P0091 OX=1971410 GN=P0091_07 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84VRR5 pKa = 11.84YY6 pKa = 9.3LPSDD10 pKa = 3.51FRR12 pKa = 11.84FKK14 pKa = 11.35ADD16 pKa = 3.25FGTYY20 pKa = 9.46QSTPNKK26 pKa = 8.16FTGVSVPKK34 pKa = 10.14FVKK37 pKa = 10.38QFTLHH42 pKa = 5.9YY43 pKa = 9.16KK44 pKa = 9.46PHH46 pKa = 6.1TRR48 pKa = 11.84TLNQEE53 pKa = 3.83YY54 pKa = 10.01LAQQNGEE61 pKa = 3.95SDD63 pKa = 3.66TIVIVIRR70 pKa = 11.84HH71 pKa = 5.08NAKK74 pKa = 9.68VLEE77 pKa = 4.46GQVVTLNGTQYY88 pKa = 11.15DD89 pKa = 3.86IVRR92 pKa = 11.84ISPDD96 pKa = 3.02EE97 pKa = 3.9NFGFNHH103 pKa = 6.67YY104 pKa = 11.11DD105 pKa = 3.11FLTLKK110 pKa = 10.2KK111 pKa = 9.72RR112 pKa = 11.84KK113 pKa = 9.51KK114 pKa = 10.37VGG116 pKa = 3.08

Molecular weight: 13.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

11264

40

1593

256.0

28.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.561 ± 0.477

0.55 ± 0.124

6.401 ± 0.279

6.96 ± 0.658

4.181 ± 0.236

6.712 ± 0.513

1.42 ± 0.106

6.578 ± 0.221

8.319 ± 0.548

8.008 ± 0.389

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.176

6.268 ± 0.276

3.045 ± 0.192

4.554 ± 0.215

4.146 ± 0.255

6.578 ± 0.406

6.241 ± 0.404

5.824 ± 0.235

1.394 ± 0.158

3.933 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski