Cytospora leucostoma

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Diaporthales; Valsaceae; Cytospora

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10071 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A423VDN6|A0A423VDN6_9PEZI Uncharacterized protein OS=Cytospora leucostoma OX=1230097 GN=VPNG_10062 PE=4 SV=1
MM1 pKa = 7.7DD2 pKa = 5.41HH3 pKa = 7.24SSAAQIGDD11 pKa = 3.68EE12 pKa = 4.14SFMVAFEE19 pKa = 5.06GYY21 pKa = 9.2PEE23 pKa = 4.49PKK25 pKa = 9.5STDD28 pKa = 3.51TNHH31 pKa = 6.45SLAHH35 pKa = 6.52DD36 pKa = 5.39PILTLTFSHH45 pKa = 7.74DD46 pKa = 3.56YY47 pKa = 11.25LLGEE51 pKa = 4.13KK52 pKa = 9.95EE53 pKa = 4.06NANFHH58 pKa = 5.41PTQYY62 pKa = 10.52EE63 pKa = 4.06DD64 pKa = 5.16KK65 pKa = 10.02NPEE68 pKa = 4.11MQWLDD73 pKa = 3.5NDD75 pKa = 3.56AMNLFGDD82 pKa = 4.37GALDD86 pKa = 3.37ILLVDD91 pKa = 3.7MTKK94 pKa = 10.89EE95 pKa = 3.68NANFHH100 pKa = 5.44PTQYY104 pKa = 10.52EE105 pKa = 4.06DD106 pKa = 5.16KK107 pKa = 10.02NPEE110 pKa = 4.11MQWLDD115 pKa = 3.5NDD117 pKa = 3.56AMNLFGDD124 pKa = 4.37GALDD128 pKa = 3.37ILLVDD133 pKa = 3.86MTTEE137 pKa = 3.85NTNFHH142 pKa = 6.36PMQYY146 pKa = 10.02EE147 pKa = 4.02DD148 pKa = 5.38KK149 pKa = 10.1NPEE152 pKa = 4.11MQWLDD157 pKa = 3.5NDD159 pKa = 3.56AMNLFGDD166 pKa = 4.37GALDD170 pKa = 3.45ILLADD175 pKa = 3.71ISEE178 pKa = 4.43EE179 pKa = 3.78NANIHH184 pKa = 4.69VTYY187 pKa = 10.83DD188 pKa = 3.36EE189 pKa = 4.93DD190 pKa = 4.32EE191 pKa = 4.5NPEE194 pKa = 4.08MLWVDD199 pKa = 3.91NYY201 pKa = 11.87AMYY204 pKa = 10.56LYY206 pKa = 10.89GDD208 pKa = 4.14GDD210 pKa = 3.49IGDD213 pKa = 4.37IDD215 pKa = 4.46SLLADD220 pKa = 3.61MAEE223 pKa = 4.32EE224 pKa = 4.03NTDD227 pKa = 2.81IHH229 pKa = 6.22ATSSQNRR236 pKa = 11.84QPQQ239 pKa = 2.98

Molecular weight: 27.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A423XJN6|A0A423XJN6_9PEZI Uncharacterized protein OS=Cytospora leucostoma OX=1230097 GN=VPNG_02873 PE=4 SV=1
MM1 pKa = 7.67SSTSTQRR8 pKa = 11.84RR9 pKa = 11.84LLARR13 pKa = 11.84LAQRR17 pKa = 11.84GQWRR21 pKa = 11.84IASTHH26 pKa = 5.42VSPSLAPRR34 pKa = 11.84HH35 pKa = 6.4HH36 pKa = 5.8YY37 pKa = 8.46QQNNILRR44 pKa = 11.84QRR46 pKa = 11.84PGRR49 pKa = 11.84ISPTPSLRR57 pKa = 11.84GFSTSPRR64 pKa = 11.84LLQEE68 pKa = 3.64QHH70 pKa = 5.61QQQQTPPPTSAHH82 pKa = 5.66VASSSSPSPTPGSPPTRR99 pKa = 11.84SSASSSSSTPSSPQPADD116 pKa = 3.1KK117 pKa = 10.59RR118 pKa = 11.84PCRR121 pKa = 11.84RR122 pKa = 11.84PRR124 pKa = 11.84LVSTLLLLLLGLTAGTATRR143 pKa = 11.84AVLAPPPPLIPGSDD157 pKa = 3.63ADD159 pKa = 3.75TSLAADD165 pKa = 3.37VRR167 pKa = 11.84AAAQHH172 pKa = 6.05LPLVRR177 pKa = 11.84QLQTDD182 pKa = 3.88PDD184 pKa = 3.41WCLRR188 pKa = 11.84HH189 pKa = 6.08HH190 pKa = 7.06DD191 pKa = 3.93AYY193 pKa = 10.38EE194 pKa = 4.27GVPAAQRR201 pKa = 11.84AGRR204 pKa = 11.84ITTGALGGSRR214 pKa = 11.84GVGAYY219 pKa = 7.19QHH221 pKa = 5.76VWHH224 pKa = 6.81NARR227 pKa = 11.84TGEE230 pKa = 4.1ALAVIYY236 pKa = 10.31FGTGTIGWPGVVHH249 pKa = 6.56GGVIATILDD258 pKa = 3.62EE259 pKa = 4.64HH260 pKa = 6.47SARR263 pKa = 11.84AALGDD268 pKa = 3.59PSALVGGAKK277 pKa = 10.27GLLTAKK283 pKa = 10.59LDD285 pKa = 3.53ITYY288 pKa = 10.2KK289 pKa = 10.63RR290 pKa = 11.84PTLSSDD296 pKa = 3.97FYY298 pKa = 10.88VVRR301 pKa = 11.84ASAVPEE307 pKa = 4.28GEE309 pKa = 3.94LAEE312 pKa = 4.17EE313 pKa = 4.28EE314 pKa = 4.02RR315 pKa = 11.84GKK317 pKa = 10.41RR318 pKa = 11.84ARR320 pKa = 11.84KK321 pKa = 8.25VWVDD325 pKa = 3.14ARR327 pKa = 11.84IEE329 pKa = 4.23TVEE332 pKa = 4.0GQTCVASRR340 pKa = 11.84ALFVTPKK347 pKa = 10.65GVDD350 pKa = 3.25LRR352 pKa = 11.84AIPEE356 pKa = 4.03NFF358 pKa = 3.11

Molecular weight: 38.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10071

0

10071

5010871

49

5411

497.6

54.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.125 ± 0.023

1.08 ± 0.009

5.994 ± 0.017

6.291 ± 0.022

3.524 ± 0.014

7.371 ± 0.025

2.351 ± 0.01

4.596 ± 0.015

4.678 ± 0.021

8.752 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.028 ± 0.01

3.496 ± 0.013

6.109 ± 0.028

3.957 ± 0.017

6.195 ± 0.021

7.946 ± 0.03

5.986 ± 0.019

6.331 ± 0.019

1.462 ± 0.01

2.729 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski