Facklamia ignava CCUG 37419

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Aerococcaceae; Facklamia; Facklamia ignava

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K1MM40|K1MM40_9LACT Pyruvate kinase OS=Facklamia ignava CCUG 37419 OX=883112 GN=HMPREF9707_00688 PE=3 SV=1
MM1 pKa = 7.43FFNINLLFNGNCQEE15 pKa = 3.86AFEE18 pKa = 5.2FYY20 pKa = 10.49HH21 pKa = 6.53QILGGEE27 pKa = 4.37AFNPTITYY35 pKa = 10.8GEE37 pKa = 4.35IDD39 pKa = 3.9PEE41 pKa = 4.43ISDD44 pKa = 3.66EE45 pKa = 4.42LKK47 pKa = 11.0DD48 pKa = 3.64KK49 pKa = 10.67IYY51 pKa = 9.91YY52 pKa = 9.22TEE54 pKa = 4.56LSFGDD59 pKa = 3.64FTLRR63 pKa = 11.84GQDD66 pKa = 3.29EE67 pKa = 4.33ALSGSFLDD75 pKa = 3.91RR76 pKa = 11.84TNTIINLDD84 pKa = 3.74FDD86 pKa = 4.22SSLVGEE92 pKa = 5.09ALYY95 pKa = 10.68QDD97 pKa = 4.63LSDD100 pKa = 3.99GGTIIEE106 pKa = 4.78PLDD109 pKa = 3.57TLIDD113 pKa = 3.81GVQYY117 pKa = 11.3SKK119 pKa = 11.05FIDD122 pKa = 3.39KK123 pKa = 10.77FGITWEE129 pKa = 4.02ILIHH133 pKa = 6.11II134 pKa = 5.11

Molecular weight: 15.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K1M3K7|K1M3K7_9LACT Phosphoesterase OS=Facklamia ignava CCUG 37419 OX=883112 GN=HMPREF9707_00776 PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.67QPKK9 pKa = 9.49KK10 pKa = 8.97RR11 pKa = 11.84KK12 pKa = 8.49NKK14 pKa = 9.15KK15 pKa = 7.58VHH17 pKa = 5.61GFRR20 pKa = 11.84KK21 pKa = 10.01RR22 pKa = 11.84MSTKK26 pKa = 9.21NGRR29 pKa = 11.84RR30 pKa = 11.84VLSKK34 pKa = 10.33RR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 10.37GRR40 pKa = 11.84KK41 pKa = 8.47RR42 pKa = 11.84LAVV45 pKa = 3.41

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1621

0

1621

507603

24

2183

313.1

35.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.874 ± 0.062

0.626 ± 0.016

5.654 ± 0.047

7.624 ± 0.073

4.018 ± 0.048

6.277 ± 0.063

2.253 ± 0.033

8.05 ± 0.049

5.311 ± 0.064

9.785 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.03

4.556 ± 0.044

3.669 ± 0.033

4.991 ± 0.057

4.451 ± 0.046

5.978 ± 0.043

5.52 ± 0.049

6.705 ± 0.053

0.879 ± 0.019

4.114 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski