Amino acid dipepetide frequency for Staphylococcus phage SMSAP5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.176AlaAla: 3.176 ± 0.8
0.244AlaCys: 0.244 ± 0.127
2.932AlaAsp: 2.932 ± 0.414
4.561AlaGlu: 4.561 ± 0.498
1.873AlaPhe: 1.873 ± 0.294
3.828AlaGly: 3.828 ± 0.712
1.059AlaHis: 1.059 ± 0.3
4.805AlaIle: 4.805 ± 0.643
6.353AlaLys: 6.353 ± 1.109
5.946AlaLeu: 5.946 ± 0.979
1.547AlaMet: 1.547 ± 0.352
4.724AlaAsn: 4.724 ± 0.86
1.547AlaPro: 1.547 ± 0.418
1.629AlaGln: 1.629 ± 0.398
2.851AlaArg: 2.851 ± 0.383
4.724AlaSer: 4.724 ± 0.775
2.851AlaThr: 2.851 ± 0.506
3.014AlaVal: 3.014 ± 0.462
1.14AlaTrp: 1.14 ± 0.392
2.932AlaTyr: 2.932 ± 0.546
0.0AlaXaa: 0.0 ± 0.0
Cys
0.244CysAla: 0.244 ± 0.143
0.0CysCys: 0.0 ± 0.0
0.081CysAsp: 0.081 ± 0.087
0.326CysGlu: 0.326 ± 0.169
0.326CysPhe: 0.326 ± 0.171
0.244CysGly: 0.244 ± 0.155
0.163CysHis: 0.163 ± 0.113
0.407CysIle: 0.407 ± 0.167
0.489CysLys: 0.489 ± 0.211
0.57CysLeu: 0.57 ± 0.235
0.081CysMet: 0.081 ± 0.065
0.163CysAsn: 0.163 ± 0.137
0.081CysPro: 0.081 ± 0.072
0.244CysGln: 0.244 ± 0.126
0.326CysArg: 0.326 ± 0.162
0.244CysSer: 0.244 ± 0.155
0.244CysThr: 0.244 ± 0.141
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.407CysTyr: 0.407 ± 0.197
0.0CysXaa: 0.0 ± 0.0
Asp
2.932AspAla: 2.932 ± 0.619
0.326AspCys: 0.326 ± 0.17
3.747AspAsp: 3.747 ± 0.72
5.294AspGlu: 5.294 ± 0.917
4.072AspPhe: 4.072 ± 0.67
3.747AspGly: 3.747 ± 0.651
0.57AspHis: 0.57 ± 0.162
5.375AspIle: 5.375 ± 0.533
6.679AspLys: 6.679 ± 0.64
5.783AspLeu: 5.783 ± 0.614
1.955AspMet: 1.955 ± 0.393
2.851AspAsn: 2.851 ± 0.433
1.059AspPro: 1.059 ± 0.311
1.629AspGln: 1.629 ± 0.336
2.118AspArg: 2.118 ± 0.391
3.421AspSer: 3.421 ± 0.614
3.747AspThr: 3.747 ± 0.534
4.561AspVal: 4.561 ± 0.575
0.814AspTrp: 0.814 ± 0.249
2.606AspTyr: 2.606 ± 0.449
0.0AspXaa: 0.0 ± 0.0
Glu
5.05GluAla: 5.05 ± 0.494
0.407GluCys: 0.407 ± 0.205
4.887GluAsp: 4.887 ± 0.898
7.737GluGlu: 7.737 ± 1.485
3.584GluPhe: 3.584 ± 0.677
3.339GluGly: 3.339 ± 0.544
1.14GluHis: 1.14 ± 0.341
5.457GluIle: 5.457 ± 0.998
7.982GluLys: 7.982 ± 1.114
8.145GluLeu: 8.145 ± 0.921
2.525GluMet: 2.525 ± 0.481
4.805GluAsn: 4.805 ± 0.679
1.303GluPro: 1.303 ± 0.338
2.769GluGln: 2.769 ± 0.495
3.014GluArg: 3.014 ± 0.582
3.502GluSer: 3.502 ± 0.61
4.235GluThr: 4.235 ± 0.561
3.665GluVal: 3.665 ± 0.49
1.222GluTrp: 1.222 ± 0.216
2.932GluTyr: 2.932 ± 0.587
0.0GluXaa: 0.0 ± 0.0
Phe
2.036PheAla: 2.036 ± 0.527
0.407PheCys: 0.407 ± 0.201
3.339PheAsp: 3.339 ± 0.557
3.258PheGlu: 3.258 ± 0.503
1.059PhePhe: 1.059 ± 0.279
2.688PheGly: 2.688 ± 0.543
0.57PheHis: 0.57 ± 0.19
3.502PheIle: 3.502 ± 0.564
5.131PheLys: 5.131 ± 0.77
2.118PheLeu: 2.118 ± 0.46
0.733PheMet: 0.733 ± 0.186
3.828PheAsn: 3.828 ± 0.588
1.14PhePro: 1.14 ± 0.377
1.059PheGln: 1.059 ± 0.297
1.222PheArg: 1.222 ± 0.288
2.118PheSer: 2.118 ± 0.393
1.792PheThr: 1.792 ± 0.334
1.71PheVal: 1.71 ± 0.325
0.244PheTrp: 0.244 ± 0.127
1.873PheTyr: 1.873 ± 0.388
0.0PheXaa: 0.0 ± 0.0
Gly
4.724GlyAla: 4.724 ± 0.944
0.244GlyCys: 0.244 ± 0.121
3.991GlyAsp: 3.991 ± 0.505
3.665GlyGlu: 3.665 ± 0.469
2.769GlyPhe: 2.769 ± 0.439
5.783GlyGly: 5.783 ± 1.139
1.466GlyHis: 1.466 ± 0.435
3.421GlyIle: 3.421 ± 0.514
6.19GlyLys: 6.19 ± 0.595
5.457GlyLeu: 5.457 ± 0.903
1.222GlyMet: 1.222 ± 0.44
3.258GlyAsn: 3.258 ± 0.646
0.977GlyPro: 0.977 ± 0.223
1.792GlyGln: 1.792 ± 0.445
2.362GlyArg: 2.362 ± 0.565
3.421GlySer: 3.421 ± 0.652
3.665GlyThr: 3.665 ± 0.678
3.991GlyVal: 3.991 ± 0.77
0.896GlyTrp: 0.896 ± 0.328
2.362GlyTyr: 2.362 ± 0.504
0.0GlyXaa: 0.0 ± 0.0
His
0.977HisAla: 0.977 ± 0.283
0.081HisCys: 0.081 ± 0.072
0.896HisAsp: 0.896 ± 0.3
1.059HisGlu: 1.059 ± 0.215
0.977HisPhe: 0.977 ± 0.392
1.14HisGly: 1.14 ± 0.301
0.489HisHis: 0.489 ± 0.197
1.14HisIle: 1.14 ± 0.366
1.303HisLys: 1.303 ± 0.262
1.629HisLeu: 1.629 ± 0.279
0.326HisMet: 0.326 ± 0.188
0.977HisAsn: 0.977 ± 0.26
0.977HisPro: 0.977 ± 0.184
0.489HisGln: 0.489 ± 0.19
0.652HisArg: 0.652 ± 0.223
1.14HisSer: 1.14 ± 0.241
1.222HisThr: 1.222 ± 0.443
0.733HisVal: 0.733 ± 0.299
0.081HisTrp: 0.081 ± 0.083
1.222HisTyr: 1.222 ± 0.286
0.0HisXaa: 0.0 ± 0.0
Ile
4.235IleAla: 4.235 ± 0.594
0.326IleCys: 0.326 ± 0.173
5.213IleAsp: 5.213 ± 0.776
5.701IleGlu: 5.701 ± 0.633
2.443IlePhe: 2.443 ± 0.6
3.502IleGly: 3.502 ± 0.587
1.873IleHis: 1.873 ± 0.402
4.154IleIle: 4.154 ± 0.765
7.493IleLys: 7.493 ± 0.935
4.561IleLeu: 4.561 ± 0.762
1.71IleMet: 1.71 ± 0.371
4.887IleAsn: 4.887 ± 0.654
2.362IlePro: 2.362 ± 0.39
1.955IleGln: 1.955 ± 0.323
2.932IleArg: 2.932 ± 0.411
4.561IleSer: 4.561 ± 0.509
3.909IleThr: 3.909 ± 0.571
3.747IleVal: 3.747 ± 0.673
0.652IleTrp: 0.652 ± 0.276
2.525IleTyr: 2.525 ± 0.461
0.0IleXaa: 0.0 ± 0.0
Lys
8.308LysAla: 8.308 ± 1.058
0.326LysCys: 0.326 ± 0.172
5.783LysAsp: 5.783 ± 0.646
8.552LysGlu: 8.552 ± 1.034
1.955LysPhe: 1.955 ± 0.389
5.538LysGly: 5.538 ± 0.848
1.792LysHis: 1.792 ± 0.431
6.027LysIle: 6.027 ± 0.697
7.656LysLys: 7.656 ± 1.125
7.819LysLeu: 7.819 ± 0.861
3.014LysMet: 3.014 ± 0.549
6.19LysAsn: 6.19 ± 0.911
2.606LysPro: 2.606 ± 0.535
4.398LysGln: 4.398 ± 0.629
3.828LysArg: 3.828 ± 0.785
5.946LysSer: 5.946 ± 1.284
4.968LysThr: 4.968 ± 0.664
5.213LysVal: 5.213 ± 0.683
1.955LysTrp: 1.955 ± 0.428
4.561LysTyr: 4.561 ± 0.636
0.0LysXaa: 0.0 ± 0.0
Leu
4.805LeuAla: 4.805 ± 0.767
0.326LeuCys: 0.326 ± 0.175
5.864LeuAsp: 5.864 ± 1.07
6.108LeuGlu: 6.108 ± 0.672
2.118LeuPhe: 2.118 ± 0.298
4.398LeuGly: 4.398 ± 0.937
1.222LeuHis: 1.222 ± 0.435
4.887LeuIle: 4.887 ± 0.626
8.226LeuLys: 8.226 ± 1.301
6.76LeuLeu: 6.76 ± 0.952
2.118LeuMet: 2.118 ± 0.361
6.271LeuAsn: 6.271 ± 0.745
3.258LeuPro: 3.258 ± 0.644
3.014LeuGln: 3.014 ± 0.653
3.665LeuArg: 3.665 ± 0.516
5.946LeuSer: 5.946 ± 0.787
5.294LeuThr: 5.294 ± 0.599
3.991LeuVal: 3.991 ± 0.414
0.163LeuTrp: 0.163 ± 0.113
3.584LeuTyr: 3.584 ± 0.854
0.0LeuXaa: 0.0 ± 0.0
Met
1.222MetAla: 1.222 ± 0.295
0.244MetCys: 0.244 ± 0.143
1.222MetAsp: 1.222 ± 0.414
1.385MetGlu: 1.385 ± 0.319
0.814MetPhe: 0.814 ± 0.215
1.547MetGly: 1.547 ± 0.498
0.407MetHis: 0.407 ± 0.192
1.222MetIle: 1.222 ± 0.255
2.932MetLys: 2.932 ± 0.548
2.199MetLeu: 2.199 ± 0.556
0.489MetMet: 0.489 ± 0.189
1.873MetAsn: 1.873 ± 0.451
0.814MetPro: 0.814 ± 0.203
2.036MetGln: 2.036 ± 0.541
1.303MetArg: 1.303 ± 0.294
2.036MetSer: 2.036 ± 0.385
2.362MetThr: 2.362 ± 0.354
0.977MetVal: 0.977 ± 0.227
0.244MetTrp: 0.244 ± 0.143
0.977MetTyr: 0.977 ± 0.262
0.0MetXaa: 0.0 ± 0.0
Asn
3.502AsnAla: 3.502 ± 0.589
0.081AsnCys: 0.081 ± 0.072
3.991AsnAsp: 3.991 ± 0.468
4.642AsnGlu: 4.642 ± 0.784
1.71AsnPhe: 1.71 ± 0.322
4.317AsnGly: 4.317 ± 0.606
1.385AsnHis: 1.385 ± 0.342
4.48AsnIle: 4.48 ± 0.479
6.679AsnLys: 6.679 ± 0.712
5.05AsnLeu: 5.05 ± 0.626
1.385AsnMet: 1.385 ± 0.315
3.828AsnAsn: 3.828 ± 0.805
2.443AsnPro: 2.443 ± 0.406
2.932AsnGln: 2.932 ± 0.514
2.688AsnArg: 2.688 ± 0.504
4.48AsnSer: 4.48 ± 0.62
4.317AsnThr: 4.317 ± 0.49
3.339AsnVal: 3.339 ± 0.61
1.222AsnTrp: 1.222 ± 0.383
2.932AsnTyr: 2.932 ± 0.555
0.0AsnXaa: 0.0 ± 0.0
Pro
1.059ProAla: 1.059 ± 0.221
0.244ProCys: 0.244 ± 0.123
1.385ProAsp: 1.385 ± 0.379
2.118ProGlu: 2.118 ± 0.415
1.629ProPhe: 1.629 ± 0.357
1.792ProGly: 1.792 ± 0.36
0.244ProHis: 0.244 ± 0.13
1.873ProIle: 1.873 ± 0.522
2.525ProLys: 2.525 ± 0.536
2.606ProLeu: 2.606 ± 0.573
0.896ProMet: 0.896 ± 0.271
2.118ProAsn: 2.118 ± 0.386
0.733ProPro: 0.733 ± 0.317
1.14ProGln: 1.14 ± 0.251
1.303ProArg: 1.303 ± 0.288
2.199ProSer: 2.199 ± 0.318
1.547ProThr: 1.547 ± 0.266
1.385ProVal: 1.385 ± 0.394
0.244ProTrp: 0.244 ± 0.14
0.977ProTyr: 0.977 ± 0.307
0.0ProXaa: 0.0 ± 0.0
Gln
2.851GlnAla: 2.851 ± 0.373
0.163GlnCys: 0.163 ± 0.11
2.281GlnAsp: 2.281 ± 0.443
2.525GlnGlu: 2.525 ± 0.47
1.466GlnPhe: 1.466 ± 0.303
2.525GlnGly: 2.525 ± 0.473
0.489GlnHis: 0.489 ± 0.176
2.932GlnIle: 2.932 ± 0.532
2.851GlnLys: 2.851 ± 0.422
3.421GlnLeu: 3.421 ± 0.513
0.896GlnMet: 0.896 ± 0.292
2.118GlnAsn: 2.118 ± 0.503
1.059GlnPro: 1.059 ± 0.276
1.303GlnGln: 1.303 ± 0.381
2.118GlnArg: 2.118 ± 0.376
2.281GlnSer: 2.281 ± 0.334
1.629GlnThr: 1.629 ± 0.388
1.792GlnVal: 1.792 ± 0.48
0.489GlnTrp: 0.489 ± 0.194
1.466GlnTyr: 1.466 ± 0.328
0.0GlnXaa: 0.0 ± 0.0
Arg
2.525ArgAla: 2.525 ± 0.479
0.0ArgCys: 0.0 ± 0.0
3.339ArgAsp: 3.339 ± 0.363
2.525ArgGlu: 2.525 ± 0.51
2.281ArgPhe: 2.281 ± 0.42
2.036ArgGly: 2.036 ± 0.384
0.814ArgHis: 0.814 ± 0.262
3.747ArgIle: 3.747 ± 0.64
3.991ArgLys: 3.991 ± 0.528
3.584ArgLeu: 3.584 ± 0.689
1.059ArgMet: 1.059 ± 0.273
2.769ArgAsn: 2.769 ± 0.524
0.733ArgPro: 0.733 ± 0.331
1.385ArgGln: 1.385 ± 0.281
2.036ArgArg: 2.036 ± 0.468
1.547ArgSer: 1.547 ± 0.4
2.443ArgThr: 2.443 ± 0.441
2.199ArgVal: 2.199 ± 0.392
0.244ArgTrp: 0.244 ± 0.142
2.362ArgTyr: 2.362 ± 0.479
0.0ArgXaa: 0.0 ± 0.0
Ser
4.154SerAla: 4.154 ± 0.801
0.244SerCys: 0.244 ± 0.135
4.642SerAsp: 4.642 ± 0.586
5.05SerGlu: 5.05 ± 0.739
2.688SerPhe: 2.688 ± 0.506
4.48SerGly: 4.48 ± 0.905
0.57SerHis: 0.57 ± 0.218
3.584SerIle: 3.584 ± 0.376
6.027SerLys: 6.027 ± 1.124
4.398SerLeu: 4.398 ± 0.496
1.71SerMet: 1.71 ± 0.378
4.805SerAsn: 4.805 ± 0.69
1.71SerPro: 1.71 ± 0.368
3.095SerGln: 3.095 ± 0.503
2.281SerArg: 2.281 ± 0.393
3.909SerSer: 3.909 ± 0.711
3.502SerThr: 3.502 ± 0.545
3.747SerVal: 3.747 ± 0.569
0.896SerTrp: 0.896 ± 0.195
1.792SerTyr: 1.792 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
3.584ThrAla: 3.584 ± 0.543
0.244ThrCys: 0.244 ± 0.127
3.502ThrAsp: 3.502 ± 0.587
4.398ThrGlu: 4.398 ± 0.613
2.851ThrPhe: 2.851 ± 0.41
4.317ThrGly: 4.317 ± 0.644
1.385ThrHis: 1.385 ± 0.384
4.561ThrIle: 4.561 ± 0.679
5.538ThrLys: 5.538 ± 0.706
4.154ThrLeu: 4.154 ± 0.614
1.14ThrMet: 1.14 ± 0.365
3.584ThrAsn: 3.584 ± 0.586
2.199ThrPro: 2.199 ± 0.325
1.385ThrGln: 1.385 ± 0.327
1.71ThrArg: 1.71 ± 0.309
3.258ThrSer: 3.258 ± 0.548
2.932ThrThr: 2.932 ± 0.581
3.991ThrVal: 3.991 ± 0.494
0.326ThrTrp: 0.326 ± 0.218
1.955ThrTyr: 1.955 ± 0.435
0.0ThrXaa: 0.0 ± 0.0
Val
3.258ValAla: 3.258 ± 0.581
0.244ValCys: 0.244 ± 0.146
3.421ValAsp: 3.421 ± 0.505
5.131ValGlu: 5.131 ± 0.621
2.199ValPhe: 2.199 ± 0.461
3.421ValGly: 3.421 ± 0.61
0.896ValHis: 0.896 ± 0.264
3.421ValIle: 3.421 ± 0.402
3.828ValLys: 3.828 ± 0.682
3.991ValLeu: 3.991 ± 0.493
1.547ValMet: 1.547 ± 0.317
3.584ValAsn: 3.584 ± 0.599
1.629ValPro: 1.629 ± 0.427
2.199ValGln: 2.199 ± 0.381
2.688ValArg: 2.688 ± 0.459
4.398ValSer: 4.398 ± 0.638
2.932ValThr: 2.932 ± 0.62
3.014ValVal: 3.014 ± 0.556
0.57ValTrp: 0.57 ± 0.208
2.036ValTyr: 2.036 ± 0.554
0.0ValXaa: 0.0 ± 0.0
Trp
0.814TrpAla: 0.814 ± 0.228
0.0TrpCys: 0.0 ± 0.0
0.489TrpAsp: 0.489 ± 0.226
0.814TrpGlu: 0.814 ± 0.31
1.629TrpPhe: 1.629 ± 0.362
0.244TrpGly: 0.244 ± 0.131
0.0TrpHis: 0.0 ± 0.0
0.896TrpIle: 0.896 ± 0.296
0.57TrpLys: 0.57 ± 0.191
1.059TrpLeu: 1.059 ± 0.249
0.407TrpMet: 0.407 ± 0.219
0.814TrpAsn: 0.814 ± 0.221
0.326TrpPro: 0.326 ± 0.225
0.407TrpGln: 0.407 ± 0.141
0.489TrpArg: 0.489 ± 0.199
1.222TrpSer: 1.222 ± 0.412
0.652TrpThr: 0.652 ± 0.191
0.733TrpVal: 0.733 ± 0.195
0.163TrpTrp: 0.163 ± 0.121
0.489TrpTyr: 0.489 ± 0.147
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.281TyrAla: 2.281 ± 0.283
0.489TyrCys: 0.489 ± 0.194
2.281TyrAsp: 2.281 ± 0.604
2.851TyrGlu: 2.851 ± 0.583
1.385TyrPhe: 1.385 ± 0.419
2.851TyrGly: 2.851 ± 0.646
0.977TyrHis: 0.977 ± 0.276
2.851TyrIle: 2.851 ± 0.614
3.991TyrLys: 3.991 ± 0.562
2.769TyrLeu: 2.769 ± 0.463
1.466TyrMet: 1.466 ± 0.295
1.955TyrAsn: 1.955 ± 0.442
1.059TyrPro: 1.059 ± 0.227
1.792TyrGln: 1.792 ± 0.289
2.036TyrArg: 2.036 ± 0.568
2.932TyrSer: 2.932 ± 0.412
2.769TyrThr: 2.769 ± 0.495
2.606TyrVal: 2.606 ± 0.496
0.652TyrTrp: 0.652 ± 0.196
1.547TyrTyr: 1.547 ± 0.428
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 42 proteins (12279 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski