Amino acid dipepetide frequency for Prevotella sp. CAG:891

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.451AlaAla: 7.451 ± 0.13	1.16AlaCys: 1.16 ± 0.04	5.571AlaAsp: 5.571 ± 0.103	5.877AlaGlu: 5.877 ± 0.112	3.435AlaPhe: 3.435 ± 0.081	5.184AlaGly: 5.184 ± 0.094	1.718AlaHis: 1.718 ± 0.058	4.374AlaIle: 4.374 ± 0.101	4.335AlaLys: 4.335 ± 0.097	7.816AlaLeu: 7.816 ± 0.127	2.093AlaMet: 2.093 ± 0.061	3.608AlaAsn: 3.608 ± 0.08	2.757AlaPro: 2.757 ± 0.069	3.851AlaGln: 3.851 ± 0.074	3.806AlaArg: 3.806 ± 0.094	4.126AlaSer: 4.126 ± 0.079	4.393AlaThr: 4.393 ± 0.076	5.359AlaVal: 5.359 ± 0.086	0.874AlaTrp: 0.874 ± 0.038	3.247AlaTyr: 3.247 ± 0.065	0.0AlaXaa: 0.0 ± 0.0
Cys	1.049CysAla: 1.049 ± 0.043	0.256CysCys: 0.256 ± 0.02	0.703CysAsp: 0.703 ± 0.034	0.739CysGlu: 0.739 ± 0.032	0.627CysPhe: 0.627 ± 0.029	1.158CysGly: 1.158 ± 0.046	0.372CysHis: 0.372 ± 0.025	1.03CysIle: 1.03 ± 0.038	0.738CysLys: 0.738 ± 0.032	1.238CysLeu: 1.238 ± 0.045	0.392CysMet: 0.392 ± 0.027	0.601CysAsn: 0.601 ± 0.03	0.652CysPro: 0.652 ± 0.032	0.453CysGln: 0.453 ± 0.027	0.725CysArg: 0.725 ± 0.029	0.765CysSer: 0.765 ± 0.036	0.749CysThr: 0.749 ± 0.038	0.961CysVal: 0.961 ± 0.041	0.166CysTrp: 0.166 ± 0.017	0.513CysTyr: 0.513 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.472AspAla: 4.472 ± 0.095	0.786AspCys: 0.786 ± 0.036	2.768AspAsp: 2.768 ± 0.072	4.053AspGlu: 4.053 ± 0.082	3.115AspPhe: 3.115 ± 0.076	3.927AspGly: 3.927 ± 0.092	0.969AspHis: 0.969 ± 0.043	3.399AspIle: 3.399 ± 0.08	3.365AspLys: 3.365 ± 0.082	4.859AspLeu: 4.859 ± 0.091	1.38AspMet: 1.38 ± 0.044	2.699AspAsn: 2.699 ± 0.069	1.786AspPro: 1.786 ± 0.058	1.263AspGln: 1.263 ± 0.046	2.464AspArg: 2.464 ± 0.06	3.074AspSer: 3.074 ± 0.083	2.901AspThr: 2.901 ± 0.066	3.621AspVal: 3.621 ± 0.072	0.755AspTrp: 0.755 ± 0.034	2.645AspTyr: 2.645 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	5.604GluAla: 5.604 ± 0.109	0.721GluCys: 0.721 ± 0.032	2.682GluAsp: 2.682 ± 0.075	4.211GluGlu: 4.211 ± 0.109	2.191GluPhe: 2.191 ± 0.057	4.315GluGly: 4.315 ± 0.095	1.423GluHis: 1.423 ± 0.044	3.631GluIle: 3.631 ± 0.073	4.156GluLys: 4.156 ± 0.087	5.963GluLeu: 5.963 ± 0.12	1.975GluMet: 1.975 ± 0.051	3.121GluAsn: 3.121 ± 0.073	1.923GluPro: 1.923 ± 0.061	2.828GluGln: 2.828 ± 0.072	3.529GluArg: 3.529 ± 0.093	2.851GluSer: 2.851 ± 0.066	3.293GluThr: 3.293 ± 0.066	4.431GluVal: 4.431 ± 0.085	0.854GluTrp: 0.854 ± 0.034	2.092GluTyr: 2.092 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	3.506PheAla: 3.506 ± 0.082	0.779PheCys: 0.779 ± 0.035	2.845PheAsp: 2.845 ± 0.069	2.443PheGlu: 2.443 ± 0.054	2.147PhePhe: 2.147 ± 0.07	3.218PheGly: 3.218 ± 0.077	0.961PheHis: 0.961 ± 0.037	2.739PheIle: 2.739 ± 0.066	2.369PheLys: 2.369 ± 0.066	3.88PheLeu: 3.88 ± 0.08	1.246PheMet: 1.246 ± 0.041	2.278PheAsn: 2.278 ± 0.062	1.549PhePro: 1.549 ± 0.048	1.296PheGln: 1.296 ± 0.047	2.169PheArg: 2.169 ± 0.062	3.144PheSer: 3.144 ± 0.082	2.942PheThr: 2.942 ± 0.065	3.158PheVal: 3.158 ± 0.074	0.451PheTrp: 0.451 ± 0.027	1.795PheTyr: 1.795 ± 0.056	0.001PheXaa: 0.001 ± 0.001
Gly	4.838GlyAla: 4.838 ± 0.094	1.025GlyCys: 1.025 ± 0.042	3.183GlyAsp: 3.183 ± 0.064	3.904GlyGlu: 3.904 ± 0.083	3.189GlyPhe: 3.189 ± 0.073	4.774GlyGly: 4.774 ± 0.112	1.467GlyHis: 1.467 ± 0.046	4.765GlyIle: 4.765 ± 0.101	4.919GlyLys: 4.919 ± 0.104	5.95GlyLeu: 5.95 ± 0.094	2.098GlyMet: 2.098 ± 0.053	3.498GlyAsn: 3.498 ± 0.083	1.421GlyPro: 1.421 ± 0.05	2.325GlyGln: 2.325 ± 0.078	3.293GlyArg: 3.293 ± 0.065	3.867GlySer: 3.867 ± 0.088	4.08GlyThr: 4.08 ± 0.079	4.958GlyVal: 4.958 ± 0.096	0.955GlyTrp: 0.955 ± 0.041	3.043GlyTyr: 3.043 ± 0.067	0.007GlyXaa: 0.007 ± 0.003
His	1.664HisAla: 1.664 ± 0.058	0.352HisCys: 0.352 ± 0.021	1.12HisAsp: 1.12 ± 0.046	1.228HisGlu: 1.228 ± 0.041	1.366HisPhe: 1.366 ± 0.044	1.394HisGly: 1.394 ± 0.047	0.647HisHis: 0.647 ± 0.035	1.634HisIle: 1.634 ± 0.049	1.059HisLys: 1.059 ± 0.04	2.156HisLeu: 2.156 ± 0.064	0.391HisMet: 0.391 ± 0.023	1.045HisAsn: 1.045 ± 0.035	1.128HisPro: 1.128 ± 0.044	0.718HisGln: 0.718 ± 0.036	1.215HisArg: 1.215 ± 0.047	1.32HisSer: 1.32 ± 0.044	1.411HisThr: 1.411 ± 0.044	1.349HisVal: 1.349 ± 0.047	0.281HisTrp: 0.281 ± 0.021	0.976HisTyr: 0.976 ± 0.04	0.0HisXaa: 0.0 ± 0.0
Ile	4.95IleAla: 4.95 ± 0.101	1.026IleCys: 1.026 ± 0.044	4.072IleAsp: 4.072 ± 0.089	4.174IleGlu: 4.174 ± 0.086	2.305IlePhe: 2.305 ± 0.061	3.985IleGly: 3.985 ± 0.091	1.336IleHis: 1.336 ± 0.054	3.638IleIle: 3.638 ± 0.096	3.465IleLys: 3.465 ± 0.078	4.811IleLeu: 4.811 ± 0.116	1.35IleMet: 1.35 ± 0.047	2.913IleAsn: 2.913 ± 0.062	2.653IlePro: 2.653 ± 0.065	1.957IleGln: 1.957 ± 0.049	2.834IleArg: 2.834 ± 0.06	3.985IleSer: 3.985 ± 0.08	3.53IleThr: 3.53 ± 0.073	4.238IleVal: 4.238 ± 0.088	0.544IleTrp: 0.544 ± 0.029	2.338IleTyr: 2.338 ± 0.056	0.0IleXaa: 0.0 ± 0.0
Lys	5.011LysAla: 5.011 ± 0.103	0.587LysCys: 0.587 ± 0.036	3.075LysAsp: 3.075 ± 0.081	4.086LysGlu: 4.086 ± 0.096	2.149LysPhe: 2.149 ± 0.054	4.119LysGly: 4.119 ± 0.092	1.387LysHis: 1.387 ± 0.038	3.199LysIle: 3.199 ± 0.078	4.077LysLys: 4.077 ± 0.097	5.346LysLeu: 5.346 ± 0.09	1.883LysMet: 1.883 ± 0.048	3.173LysAsn: 3.173 ± 0.079	2.319LysPro: 2.319 ± 0.06	2.943LysGln: 2.943 ± 0.062	3.178LysArg: 3.178 ± 0.07	3.05LysSer: 3.05 ± 0.069	3.117LysThr: 3.117 ± 0.066	4.107LysVal: 4.107 ± 0.087	0.635LysTrp: 0.635 ± 0.028	2.402LysTyr: 2.402 ± 0.071	0.0LysXaa: 0.0 ± 0.0
Leu	7.083LeuAla: 7.083 ± 0.113	1.566LeuCys: 1.566 ± 0.048	4.561LeuAsp: 4.561 ± 0.099	4.522LeuGlu: 4.522 ± 0.102	4.244LeuPhe: 4.244 ± 0.096	5.79LeuGly: 5.79 ± 0.099	2.314LeuHis: 2.314 ± 0.063	5.183LeuIle: 5.183 ± 0.118	5.737LeuLys: 5.737 ± 0.102	9.41LeuLeu: 9.41 ± 0.168	2.575LeuMet: 2.575 ± 0.066	4.636LeuAsn: 4.636 ± 0.086	4.627LeuPro: 4.627 ± 0.086	4.065LeuGln: 4.065 ± 0.088	5.159LeuArg: 5.159 ± 0.095	6.394LeuSer: 6.394 ± 0.114	6.046LeuThr: 6.046 ± 0.102	5.406LeuVal: 5.406 ± 0.106	0.988LeuTrp: 0.988 ± 0.045	3.321LeuTyr: 3.321 ± 0.069	0.001LeuXaa: 0.001 ± 0.001
Met	2.39MetAla: 2.39 ± 0.064	0.271MetCys: 0.271 ± 0.02	1.299MetAsp: 1.299 ± 0.043	1.46MetGlu: 1.46 ± 0.043	0.999MetPhe: 0.999 ± 0.043	1.954MetGly: 1.954 ± 0.052	0.551MetHis: 0.551 ± 0.029	1.329MetIle: 1.329 ± 0.045	2.136MetLys: 2.136 ± 0.048	2.774MetLeu: 2.774 ± 0.069	0.873MetMet: 0.873 ± 0.041	1.532MetAsn: 1.532 ± 0.047	1.4MetPro: 1.4 ± 0.051	1.334MetGln: 1.334 ± 0.037	1.413MetArg: 1.413 ± 0.043	1.516MetSer: 1.516 ± 0.047	1.589MetThr: 1.589 ± 0.047	1.713MetVal: 1.713 ± 0.051	0.237MetTrp: 0.237 ± 0.021	0.723MetTyr: 0.723 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.927AsnAla: 3.927 ± 0.082	0.574AsnCys: 0.574 ± 0.031	2.615AsnAsp: 2.615 ± 0.075	3.22AsnGlu: 3.22 ± 0.074	2.247AsnPhe: 2.247 ± 0.057	3.75AsnGly: 3.75 ± 0.092	1.072AsnHis: 1.072 ± 0.038	3.08AsnIle: 3.08 ± 0.069	2.784AsnLys: 2.784 ± 0.076	4.485AsnLeu: 4.485 ± 0.101	1.323AsnMet: 1.323 ± 0.042	2.355AsnAsn: 2.355 ± 0.067	2.457AsnPro: 2.457 ± 0.056	1.671AsnGln: 1.671 ± 0.053	2.534AsnArg: 2.534 ± 0.066	2.483AsnSer: 2.483 ± 0.063	2.561AsnThr: 2.561 ± 0.067	3.173AsnVal: 3.173 ± 0.07	0.604AsnTrp: 0.604 ± 0.031	2.143AsnTyr: 2.143 ± 0.061	0.0AsnXaa: 0.0 ± 0.0
Pro	3.219ProAla: 3.219 ± 0.075	0.463ProCys: 0.463 ± 0.03	2.587ProAsp: 2.587 ± 0.071	3.297ProGlu: 3.297 ± 0.076	1.917ProPhe: 1.917 ± 0.056	2.54ProGly: 2.54 ± 0.064	0.877ProHis: 0.877 ± 0.039	2.179ProIle: 2.179 ± 0.057	1.978ProLys: 1.978 ± 0.053	3.654ProLeu: 3.654 ± 0.076	1.037ProMet: 1.037 ± 0.04	1.913ProAsn: 1.913 ± 0.058	0.901ProPro: 0.901 ± 0.051	1.707ProGln: 1.707 ± 0.051	1.539ProArg: 1.539 ± 0.053	2.139ProSer: 2.139 ± 0.069	2.41ProThr: 2.41 ± 0.064	2.743ProVal: 2.743 ± 0.064	0.465ProTrp: 0.465 ± 0.025	1.661ProTyr: 1.661 ± 0.047	0.001ProXaa: 0.001 ± 0.001
Gln	3.129GlnAla: 3.129 ± 0.065	0.46GlnCys: 0.46 ± 0.025	1.367GlnAsp: 1.367 ± 0.044	1.848GlnGlu: 1.848 ± 0.058	1.558GlnPhe: 1.558 ± 0.055	2.415GlnGly: 2.415 ± 0.067	1.057GlnHis: 1.057 ± 0.038	2.446GlnIle: 2.446 ± 0.059	2.417GlnLys: 2.417 ± 0.056	3.92GlnLeu: 3.92 ± 0.076	1.245GlnMet: 1.245 ± 0.04	1.907GlnAsn: 1.907 ± 0.057	1.74GlnPro: 1.74 ± 0.049	2.083GlnGln: 2.083 ± 0.065	2.311GlnArg: 2.311 ± 0.063	2.203GlnSer: 2.203 ± 0.056	2.626GlnThr: 2.626 ± 0.064	2.365GlnVal: 2.365 ± 0.057	0.561GlnTrp: 0.561 ± 0.031	1.441GlnTyr: 1.441 ± 0.046	0.0GlnXaa: 0.0 ± 0.0
Arg	3.328ArgAla: 3.328 ± 0.067	0.613ArgCys: 0.613 ± 0.032	2.282ArgAsp: 2.282 ± 0.056	3.097ArgGlu: 3.097 ± 0.079	2.471ArgPhe: 2.471 ± 0.063	2.776ArgGly: 2.776 ± 0.076	1.245ArgHis: 1.245 ± 0.046	3.532ArgIle: 3.532 ± 0.078	3.247ArgLys: 3.247 ± 0.081	4.889ArgLeu: 4.889 ± 0.103	1.691ArgMet: 1.691 ± 0.047	2.584ArgAsn: 2.584 ± 0.064	1.926ArgPro: 1.926 ± 0.058	2.213ArgGln: 2.213 ± 0.059	2.876ArgArg: 2.876 ± 0.079	2.78ArgSer: 2.78 ± 0.073	2.888ArgThr: 2.888 ± 0.07	3.119ArgVal: 3.119 ± 0.066	0.651ArgTrp: 0.651 ± 0.03	2.236ArgTyr: 2.236 ± 0.069	0.0ArgXaa: 0.0 ± 0.0
Ser	4.623SerAla: 4.623 ± 0.078	0.76SerCys: 0.76 ± 0.035	2.933SerAsp: 2.933 ± 0.068	3.29SerGlu: 3.29 ± 0.072	3.026SerPhe: 3.026 ± 0.081	4.049SerGly: 4.049 ± 0.077	1.29SerHis: 1.29 ± 0.049	3.732SerIle: 3.732 ± 0.085	3.087SerLys: 3.087 ± 0.087	5.786SerLeu: 5.786 ± 0.115	1.475SerMet: 1.475 ± 0.048	2.481SerAsn: 2.481 ± 0.074	2.135SerPro: 2.135 ± 0.061	2.082SerGln: 2.082 ± 0.058	2.747SerArg: 2.747 ± 0.075	3.431SerSer: 3.431 ± 0.085	3.404SerThr: 3.404 ± 0.09	4.197SerVal: 4.197 ± 0.077	0.711SerTrp: 0.711 ± 0.031	2.285SerTyr: 2.285 ± 0.082	0.0SerXaa: 0.0 ± 0.0
Thr	5.093ThrAla: 5.093 ± 0.099	0.64ThrCys: 0.64 ± 0.033	3.82ThrAsp: 3.82 ± 0.085	3.458ThrGlu: 3.458 ± 0.08	2.665ThrPhe: 2.665 ± 0.061	4.056ThrGly: 4.056 ± 0.073	1.364ThrHis: 1.364 ± 0.046	3.377ThrIle: 3.377 ± 0.068	2.65ThrLys: 2.65 ± 0.068	6.142ThrLeu: 6.142 ± 0.108	1.15ThrMet: 1.15 ± 0.042	2.49ThrAsn: 2.49 ± 0.065	3.119ThrPro: 3.119 ± 0.069	2.123ThrGln: 2.123 ± 0.058	2.369ThrArg: 2.369 ± 0.061	3.246ThrSer: 3.246 ± 0.072	3.546ThrThr: 3.546 ± 0.084	3.954ThrVal: 3.954 ± 0.099	0.604ThrTrp: 0.604 ± 0.032	2.41ThrTyr: 2.41 ± 0.073	0.001ThrXaa: 0.001 ± 0.001
Val	5.508ValAla: 5.508 ± 0.1	1.147ValCys: 1.147 ± 0.046	3.831ValAsp: 3.831 ± 0.084	3.979ValGlu: 3.979 ± 0.091	2.778ValPhe: 2.778 ± 0.072	4.522ValGly: 4.522 ± 0.111	1.194ValHis: 1.194 ± 0.042	3.905ValIle: 3.905 ± 0.079	4.352ValLys: 4.352 ± 0.082	5.72ValLeu: 5.72 ± 0.101	1.907ValMet: 1.907 ± 0.056	3.377ValAsn: 3.377 ± 0.073	2.794ValPro: 2.794 ± 0.06	2.209ValGln: 2.209 ± 0.055	3.471ValArg: 3.471 ± 0.074	4.359ValSer: 4.359 ± 0.08	3.796ValThr: 3.796 ± 0.078	5.041ValVal: 5.041 ± 0.127	0.819ValTrp: 0.819 ± 0.041	2.578ValTyr: 2.578 ± 0.075	0.001ValXaa: 0.001 ± 0.001
Trp	0.76TrpAla: 0.76 ± 0.033	0.162TrpCys: 0.162 ± 0.016	0.614TrpAsp: 0.614 ± 0.033	0.588TrpGlu: 0.588 ± 0.031	0.547TrpPhe: 0.547 ± 0.029	0.863TrpGly: 0.863 ± 0.037	0.323TrpHis: 0.323 ± 0.019	0.679TrpIle: 0.679 ± 0.032	0.777TrpLys: 0.777 ± 0.031	1.147TrpLeu: 1.147 ± 0.042	0.409TrpMet: 0.409 ± 0.025	0.726TrpAsn: 0.726 ± 0.034	0.308TrpPro: 0.308 ± 0.021	0.64TrpGln: 0.64 ± 0.032	0.644TrpArg: 0.644 ± 0.034	0.625TrpSer: 0.625 ± 0.034	0.651TrpThr: 0.651 ± 0.036	0.694TrpVal: 0.694 ± 0.032	0.175TrpTrp: 0.175 ± 0.017	0.463TrpTyr: 0.463 ± 0.029	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.426TyrAla: 3.426 ± 0.078	0.54TyrCys: 0.54 ± 0.029	2.373TyrAsp: 2.373 ± 0.057	2.319TyrGlu: 2.319 ± 0.061	1.938TyrPhe: 1.938 ± 0.05	2.771TyrGly: 2.771 ± 0.067	0.888TyrHis: 0.888 ± 0.034	2.253TyrIle: 2.253 ± 0.059	2.196TyrLys: 2.196 ± 0.058	3.679TyrLeu: 3.679 ± 0.077	0.998TyrMet: 0.998 ± 0.039	2.122TyrAsn: 2.122 ± 0.078	1.63TyrPro: 1.63 ± 0.051	1.336TyrGln: 1.336 ± 0.049	2.164TyrArg: 2.164 ± 0.058	2.166TyrSer: 2.166 ± 0.061	2.345TyrThr: 2.345 ± 0.065	2.702TyrVal: 2.702 ± 0.065	0.466TyrTrp: 0.466 ± 0.029	1.929TyrTyr: 1.929 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.004XaaArg: 0.004 ± 0.002	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.014XaaXaa: 0.014 ± 0.005

Statistics based on 2058 proteins (703646 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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