Circoviridae 20 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples; Circoviridae LDMD-2013a

Average proteome isoelectric point is 7.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TNF8|S5TNF8_9CIRC Uncharacterized protein OS=Circoviridae 20 LDMD-2013 OX=1379724 PE=4 SV=1
MM1 pKa = 7.69HH2 pKa = 7.46ILSXXIFXDD11 pKa = 5.25CIAKK15 pKa = 10.07NNSVAEE21 pKa = 4.51DD22 pKa = 4.0DD23 pKa = 4.25PLTNIIQGIDD33 pKa = 3.09NRR35 pKa = 11.84FNADD39 pKa = 3.02AGFEE43 pKa = 4.05NSIMYY48 pKa = 9.98YY49 pKa = 9.62PEE51 pKa = 4.74HH52 pKa = 6.38SHH54 pKa = 6.82VFLEE58 pKa = 3.94NWKK61 pKa = 8.43ITGKK65 pKa = 10.43RR66 pKa = 11.84SVTLHH71 pKa = 6.69PGEE74 pKa = 4.08QTTISHH80 pKa = 5.87SCSGYY85 pKa = 6.75STRR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84YY91 pKa = 10.22LILLSEE97 pKa = 4.41LSLWFII103 pKa = 4.9

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5T6B0|S5T6B0_9CIRC ATP-dependent helicase Rep OS=Circoviridae 20 LDMD-2013 OX=1379724 PE=3 SV=1
MM1 pKa = 7.39AGIVFFEE8 pKa = 4.21AAEE11 pKa = 4.27LGLPVLEE18 pKa = 4.81AAMEE22 pKa = 4.25ASEE25 pKa = 4.42FVHH28 pKa = 6.62PFARR32 pKa = 11.84QTVRR36 pKa = 11.84TQVGAGAFAAGVNKK50 pKa = 8.87LTSGPDD56 pKa = 3.32HH57 pKa = 6.69KK58 pKa = 10.41RR59 pKa = 11.84KK60 pKa = 10.73SMVKK64 pKa = 8.81TRR66 pKa = 11.84SHH68 pKa = 6.2TKK70 pKa = 10.44NEE72 pKa = 3.69LAALRR77 pKa = 11.84TIAAQNDD84 pKa = 3.71LMKK87 pKa = 9.85TQTQMRR93 pKa = 11.84TFVPTSFKK101 pKa = 10.09KK102 pKa = 10.25RR103 pKa = 11.84KK104 pKa = 9.92RR105 pKa = 11.84FDD107 pKa = 3.37KK108 pKa = 11.08LSDD111 pKa = 3.36PGIWQTRR118 pKa = 11.84RR119 pKa = 11.84QVEE122 pKa = 4.18NVILTGNTNAATYY135 pKa = 7.34TVSYY139 pKa = 9.92IGRR142 pKa = 11.84NSDD145 pKa = 4.58LLNLMDD151 pKa = 6.74DD152 pKa = 3.86EE153 pKa = 4.7FKK155 pKa = 11.08KK156 pKa = 10.68IGGSNDD162 pKa = 3.25IEE164 pKa = 4.38HH165 pKa = 6.86VDD167 pKa = 3.54YY168 pKa = 9.42TAASMAAFSPILQFKK183 pKa = 9.24IRR185 pKa = 11.84QVLHH189 pKa = 5.63MKK191 pKa = 10.38NNYY194 pKa = 9.86AYY196 pKa = 8.98TVXXYY201 pKa = 9.1FFXLYY206 pKa = 9.84CKK208 pKa = 10.39KK209 pKa = 10.74

Molecular weight: 23.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

710

103

282

177.5

20.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.915 ± 1.738

1.549 ± 0.403

5.493 ± 0.725

4.366 ± 0.621

5.07 ± 0.463

5.634 ± 0.907

2.676 ± 0.578

5.915 ± 2.169

6.901 ± 1.223

7.746 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.958 ± 0.403

4.93 ± 0.829

3.662 ± 0.359

3.803 ± 0.634

5.775 ± 0.488

7.183 ± 1.516

7.887 ± 0.649

5.352 ± 0.942

1.972 ± 0.761

3.944 ± 0.686
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski