Bacillus phage vB_Bpu_PumA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Bundooravirus; Bacillus virus PumA1

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W7N7|A0A5Q2W7N7_9CAUD Morphogenesis protein OS=Bacillus phage vB_Bpu_PumA1 OX=2662127 PE=4 SV=1
MM1 pKa = 7.56VLVTKK6 pKa = 10.6DD7 pKa = 3.67DD8 pKa = 4.08FGEE11 pKa = 4.24CTIMGSKK18 pKa = 9.96EE19 pKa = 3.63MTMDD23 pKa = 5.1DD24 pKa = 3.05IGYY27 pKa = 9.18VIGMTIEE34 pKa = 4.55EE35 pKa = 4.46IIEE38 pKa = 4.21CQFVKK43 pKa = 10.52EE44 pKa = 4.47GEE46 pKa = 3.92ADD48 pKa = 3.16YY49 pKa = 11.36DD50 pKa = 4.4AIFTEE55 pKa = 4.82LQSKK59 pKa = 9.41YY60 pKa = 10.18CSEE63 pKa = 4.54EE64 pKa = 3.86KK65 pKa = 11.02GQDD68 pKa = 3.01

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WD83|A0A5Q2WD83_9CAUD DNA polymerase OS=Bacillus phage vB_Bpu_PumA1 OX=2662127 PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 10.3KK3 pKa = 10.06VVIDD7 pKa = 4.23AGHH10 pKa = 6.39GGIDD14 pKa = 3.35SGASAGGVRR23 pKa = 11.84EE24 pKa = 3.83KK25 pKa = 10.91DD26 pKa = 2.91ITLKK30 pKa = 10.12IVKK33 pKa = 9.69LIEE36 pKa = 4.54GEE38 pKa = 4.12LVNYY42 pKa = 8.97DD43 pKa = 3.0VKK45 pKa = 11.09AIYY48 pKa = 9.04TRR50 pKa = 11.84SSDD53 pKa = 3.75TYY55 pKa = 8.26PSLSEE60 pKa = 3.72RR61 pKa = 11.84VEE63 pKa = 3.64IANRR67 pKa = 11.84NKK69 pKa = 9.55TDD71 pKa = 3.47LFVSVHH77 pKa = 5.48VNSATNKK84 pKa = 7.34TANGYY89 pKa = 7.13EE90 pKa = 4.04TLVYY94 pKa = 10.49LGLGRR99 pKa = 11.84SRR101 pKa = 11.84QGAIFHH107 pKa = 6.39KK108 pKa = 10.42HH109 pKa = 5.35LSTVNKK115 pKa = 10.38AYY117 pKa = 10.32GKK119 pKa = 9.65KK120 pKa = 10.1DD121 pKa = 3.35RR122 pKa = 11.84GIKK125 pKa = 9.05EE126 pKa = 3.52QDD128 pKa = 3.57VQVLRR133 pKa = 11.84ATKK136 pKa = 9.11MPAVLTEE143 pKa = 3.91NLFVSNEE150 pKa = 3.92YY151 pKa = 9.68EE152 pKa = 4.27RR153 pKa = 11.84KK154 pKa = 10.03LLLDD158 pKa = 4.11DD159 pKa = 5.69KK160 pKa = 11.11YY161 pKa = 11.4LQLVAKK167 pKa = 9.71AHH169 pKa = 6.62IDD171 pKa = 3.82AIVEE175 pKa = 4.03FLSLKK180 pKa = 10.41KK181 pKa = 9.52KK182 pKa = 9.37TSIEE186 pKa = 3.85NYY188 pKa = 9.62KK189 pKa = 10.35IKK191 pKa = 9.95TGDD194 pKa = 3.36TLYY197 pKa = 11.1SIAKK201 pKa = 7.97NHH203 pKa = 5.9KK204 pKa = 6.38TTVNAIQKK212 pKa = 10.13RR213 pKa = 11.84NNIKK217 pKa = 10.07NASLIRR223 pKa = 11.84AGKK226 pKa = 8.01VLKK229 pKa = 10.29IKK231 pKa = 10.57RR232 pKa = 11.84GEE234 pKa = 3.99KK235 pKa = 10.18

Molecular weight: 26.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

5748

56

613

221.1

25.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.393 ± 0.723

0.922 ± 0.167

6.246 ± 0.262

7.237 ± 0.565

4.732 ± 0.531

6.193 ± 0.619

1.688 ± 0.171

6.715 ± 0.297

7.933 ± 0.738

7.463 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.992 ± 0.355

6.768 ± 0.476

2.818 ± 0.277

3.532 ± 0.332

4.106 ± 0.292

5.898 ± 0.372

6.698 ± 0.418

6.768 ± 0.491

0.905 ± 0.151

4.993 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski