Serendipita indica (strain DSM 11827) (Root endophyte fungus) (Piriformospora indica)
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11780 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G4U0X3|G4U0X3_SERID Uncharacterized protein OS=Serendipita indica (strain DSM 11827) OX=1109443 GN=PIIN_11198 PE=4 SV=1
MM1 pKa = 7.42 AHH3 pKa = 6.67 FANPVVVDD11 pKa = 4.86 DD12 pKa = 4.13 KK13 pKa = 11.42 TFAYY17 pKa = 10.18 DD18 pKa = 3.37 EE19 pKa = 4.32 RR20 pKa = 11.84 DD21 pKa = 3.4 AYY23 pKa = 11.11 AMGNFHH29 pKa = 7.47 AGLATEE35 pKa = 4.48 ATMDD39 pKa = 4.15 DD40 pKa = 4.12 YY41 pKa = 11.85 ADD43 pKa = 4.52 EE44 pKa = 4.44 EE45 pKa = 4.37 DD46 pKa = 4.28 SPYY49 pKa = 11.32 EE50 pKa = 3.91 EE51 pKa = 4.68
Molecular weight: 5.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.77
IPC_protein 3.681
Toseland 3.49
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.935
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.656
Lehninger 3.617
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.775
Protein with the highest isoelectric point:
>tr|G4T6L9|G4T6L9_SERID F-box domain-containing protein OS=Serendipita indica (strain DSM 11827) OX=1109443 GN=PIIN_00799 PE=4 SV=1
MM1 pKa = 7.26 TLASALGLRR10 pKa = 11.84 RR11 pKa = 11.84 SPRR14 pKa = 11.84 NHH16 pKa = 7.0 RR17 pKa = 11.84 SHH19 pKa = 6.97 AANRR23 pKa = 11.84 NAGLKK28 pKa = 10.19 SALTNPNSTHH38 pKa = 7.15 AGRR41 pKa = 11.84 AQAKK45 pKa = 10.01 HH46 pKa = 5.32 EE47 pKa = 3.91 LHH49 pKa = 6.3 MRR51 pKa = 11.84 GQSAHH56 pKa = 5.8 VPLMAKK62 pKa = 9.8 VKK64 pKa = 10.07 RR65 pKa = 11.84 ALGMHH70 pKa = 4.93 QTPRR74 pKa = 11.84 HH75 pKa = 4.29 QRR77 pKa = 11.84 KK78 pKa = 9.38 VNNARR83 pKa = 11.84 ARR85 pKa = 11.84 ARR87 pKa = 11.84 RR88 pKa = 11.84 QAGTTRR94 pKa = 11.84 KK95 pKa = 5.41 THH97 pKa = 6.04 RR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 HH101 pKa = 4.66 YY102 pKa = 10.7
Molecular weight: 11.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 11.082
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.062
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.107
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11780
0
11780
5140652
10
4811
436.4
48.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.389 ± 0.019
1.139 ± 0.009
5.357 ± 0.015
5.945 ± 0.02
3.492 ± 0.014
6.392 ± 0.024
2.547 ± 0.01
5.043 ± 0.018
4.515 ± 0.017
9.252 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.112 ± 0.008
3.533 ± 0.01
6.445 ± 0.03
3.925 ± 0.014
6.36 ± 0.019
8.937 ± 0.034
6.332 ± 0.016
6.135 ± 0.016
1.44 ± 0.008
2.649 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here