Clostridium sp. CAG:343

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1443 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6NCN6|R6NCN6_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:343 OX=1262796 GN=BN615_00457 PE=4 SV=1
MM1 pKa = 7.8AYY3 pKa = 9.72KK4 pKa = 9.08ITEE7 pKa = 3.99KK8 pKa = 10.69CISCGACAVEE18 pKa = 4.94CPVGCISQGEE28 pKa = 4.3DD29 pKa = 3.19KK30 pKa = 10.96FVIDD34 pKa = 4.65ASACISCGTCAGVCPVEE51 pKa = 4.41APEE54 pKa = 4.25EE55 pKa = 4.14EE56 pKa = 4.37

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6NDW7|R6NDW7_9CLOT DUF2179 domain-containing protein OS=Clostridium sp. CAG:343 OX=1262796 GN=BN615_00797 PE=4 SV=1
MM1 pKa = 6.81YY2 pKa = 10.44LKK4 pKa = 10.5RR5 pKa = 11.84LEE7 pKa = 4.12LQGFKK12 pKa = 10.84SFADD16 pKa = 3.44KK17 pKa = 10.68TILEE21 pKa = 4.32FMPGITSVIGPNGSGKK37 pKa = 10.98SNISDD42 pKa = 3.98SIRR45 pKa = 11.84WVLRR49 pKa = 11.84RR50 pKa = 11.84AKK52 pKa = 10.64HH53 pKa = 6.0EE54 pKa = 4.24IIKK57 pKa = 9.59RR58 pKa = 11.84NKK60 pKa = 8.64VIRR63 pKa = 11.84HH64 pKa = 4.81YY65 pKa = 10.75FCRR68 pKa = 11.84NPKK71 pKa = 9.86

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1443

0

1443

406588

29

2556

281.8

32.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.82 ± 0.053

1.106 ± 0.024

5.378 ± 0.054

8.547 ± 0.077

3.941 ± 0.056

5.607 ± 0.059

1.219 ± 0.024

10.514 ± 0.085

10.555 ± 0.073

8.275 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.033

7.041 ± 0.071

2.495 ± 0.037

2.851 ± 0.034

3.257 ± 0.048

5.688 ± 0.056

5.332 ± 0.055

5.822 ± 0.047

0.631 ± 0.02

4.508 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski