Amino acid dipepetide frequency for Borrelia duttonii CR2A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.533AlaAla: 3.533 ± 0.161
0.455AlaCys: 0.455 ± 0.031
2.26AlaAsp: 2.26 ± 0.088
2.74AlaGlu: 2.74 ± 0.094
2.321AlaPhe: 2.321 ± 0.077
3.114AlaGly: 3.114 ± 0.114
0.629AlaHis: 0.629 ± 0.038
4.958AlaIle: 4.958 ± 0.113
5.071AlaLys: 5.071 ± 0.143
5.08AlaLeu: 5.08 ± 0.138
1.039AlaMet: 1.039 ± 0.046
3.145AlaAsn: 3.145 ± 0.097
0.907AlaPro: 0.907 ± 0.058
1.228AlaGln: 1.228 ± 0.065
1.757AlaArg: 1.757 ± 0.072
3.787AlaSer: 3.787 ± 0.108
2.503AlaThr: 2.503 ± 0.077
3.379AlaVal: 3.379 ± 0.139
0.241AlaTrp: 0.241 ± 0.023
2.111AlaTyr: 2.111 ± 0.082
0.0AlaXaa: 0.0 ± 0.0
Cys
0.31CysAla: 0.31 ± 0.026
0.116CysCys: 0.116 ± 0.013
0.479CysAsp: 0.479 ± 0.03
0.392CysGlu: 0.392 ± 0.028
0.473CysPhe: 0.473 ± 0.037
0.468CysGly: 0.468 ± 0.033
0.114CysHis: 0.114 ± 0.016
0.733CysIle: 0.733 ± 0.043
0.724CysLys: 0.724 ± 0.044
0.767CysLeu: 0.767 ± 0.043
0.21CysMet: 0.21 ± 0.02
0.838CysAsn: 0.838 ± 0.047
0.172CysPro: 0.172 ± 0.022
0.145CysGln: 0.145 ± 0.017
0.241CysArg: 0.241 ± 0.023
0.602CysSer: 0.602 ± 0.038
0.272CysThr: 0.272 ± 0.025
0.51CysVal: 0.51 ± 0.034
0.031CysTrp: 0.031 ± 0.007
0.43CysTyr: 0.43 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
3.17AspAla: 3.17 ± 0.131
0.328AspCys: 0.328 ± 0.025
3.373AspAsp: 3.373 ± 0.099
3.72AspGlu: 3.72 ± 0.117
3.752AspPhe: 3.752 ± 0.121
2.697AspGly: 2.697 ± 0.09
0.586AspHis: 0.586 ± 0.042
6.279AspIle: 6.279 ± 0.116
5.535AspLys: 5.535 ± 0.123
5.577AspLeu: 5.577 ± 0.117
1.19AspMet: 1.19 ± 0.05
4.22AspAsn: 4.22 ± 0.098
1.259AspPro: 1.259 ± 0.053
1.039AspGln: 1.039 ± 0.05
1.701AspArg: 1.701 ± 0.058
3.712AspSer: 3.712 ± 0.107
2.608AspThr: 2.608 ± 0.087
3.593AspVal: 3.593 ± 0.103
0.243AspTrp: 0.243 ± 0.023
2.37AspTyr: 2.37 ± 0.069
0.0AspXaa: 0.0 ± 0.0
Glu
3.415GluAla: 3.415 ± 0.104
0.441GluCys: 0.441 ± 0.034
3.415GluAsp: 3.415 ± 0.078
5.181GluGlu: 5.181 ± 0.138
3.683GluPhe: 3.683 ± 0.092
3.415GluGly: 3.415 ± 0.089
1.068GluHis: 1.068 ± 0.045
7.907GluIle: 7.907 ± 0.153
6.741GluLys: 6.741 ± 0.152
6.507GluLeu: 6.507 ± 0.161
1.522GluMet: 1.522 ± 0.057
4.75GluAsn: 4.75 ± 0.119
1.148GluPro: 1.148 ± 0.048
2.22GluGln: 2.22 ± 0.075
2.461GluArg: 2.461 ± 0.087
3.678GluSer: 3.678 ± 0.091
2.713GluThr: 2.713 ± 0.087
4.037GluVal: 4.037 ± 0.11
0.274GluTrp: 0.274 ± 0.026
2.996GluTyr: 2.996 ± 0.09
0.0GluXaa: 0.0 ± 0.0
Phe
2.321PheAla: 2.321 ± 0.077
0.604PheCys: 0.604 ± 0.041
3.263PheAsp: 3.263 ± 0.103
3.11PheGlu: 3.11 ± 0.091
3.486PhePhe: 3.486 ± 0.162
2.784PheGly: 2.784 ± 0.101
0.658PheHis: 0.658 ± 0.045
5.294PheIle: 5.294 ± 0.117
5.111PheLys: 5.111 ± 0.12
6.333PheLeu: 6.333 ± 0.139
1.286PheMet: 1.286 ± 0.058
3.834PheAsn: 3.834 ± 0.102
1.074PhePro: 1.074 ± 0.052
1.284PheGln: 1.284 ± 0.057
1.621PheArg: 1.621 ± 0.078
3.946PheSer: 3.946 ± 0.094
2.185PheThr: 2.185 ± 0.07
3.312PheVal: 3.312 ± 0.101
0.281PheTrp: 0.281 ± 0.028
2.528PheTyr: 2.528 ± 0.08
0.0PheXaa: 0.0 ± 0.0
Gly
3.399GlyAla: 3.399 ± 0.14
0.604GlyCys: 0.604 ± 0.031
3.221GlyAsp: 3.221 ± 0.09
3.161GlyGlu: 3.161 ± 0.087
3.052GlyPhe: 3.052 ± 0.099
3.562GlyGly: 3.562 ± 0.132
0.738GlyHis: 0.738 ± 0.039
6.346GlyIle: 6.346 ± 0.147
4.828GlyLys: 4.828 ± 0.114
4.472GlyLeu: 4.472 ± 0.117
1.166GlyMet: 1.166 ± 0.061
3.136GlyAsn: 3.136 ± 0.096
0.849GlyPro: 0.849 ± 0.053
1.351GlyGln: 1.351 ± 0.056
1.71GlyArg: 1.71 ± 0.064
3.315GlySer: 3.315 ± 0.083
2.621GlyThr: 2.621 ± 0.093
3.758GlyVal: 3.758 ± 0.098
0.288GlyTrp: 0.288 ± 0.026
2.022GlyTyr: 2.022 ± 0.073
0.0GlyXaa: 0.0 ± 0.0
His
0.606HisAla: 0.606 ± 0.034
0.085HisCys: 0.085 ± 0.014
0.711HisAsp: 0.711 ± 0.04
0.673HisGlu: 0.673 ± 0.043
0.802HisPhe: 0.802 ± 0.047
0.584HisGly: 0.584 ± 0.037
0.21HisHis: 0.21 ± 0.025
1.358HisIle: 1.358 ± 0.053
1.476HisLys: 1.476 ± 0.067
1.529HisLeu: 1.529 ± 0.065
0.281HisMet: 0.281 ± 0.029
1.012HisAsn: 1.012 ± 0.049
0.395HisPro: 0.395 ± 0.037
0.366HisGln: 0.366 ± 0.027
0.479HisArg: 0.479 ± 0.035
0.914HisSer: 0.914 ± 0.044
0.66HisThr: 0.66 ± 0.045
0.597HisVal: 0.597 ± 0.041
0.053HisTrp: 0.053 ± 0.011
0.689HisTyr: 0.689 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
5.357IleAla: 5.357 ± 0.122
0.885IleCys: 0.885 ± 0.045
5.896IleAsp: 5.896 ± 0.115
6.788IleGlu: 6.788 ± 0.14
5.254IlePhe: 5.254 ± 0.11
4.94IleGly: 4.94 ± 0.131
1.237IleHis: 1.237 ± 0.058
9.603IleIle: 9.603 ± 0.241
10.858IleLys: 10.858 ± 0.168
10.408IleLeu: 10.408 ± 0.182
2.082IleMet: 2.082 ± 0.08
7.501IleAsn: 7.501 ± 0.163
2.753IlePro: 2.753 ± 0.106
2.659IleGln: 2.659 ± 0.093
3.346IleArg: 3.346 ± 0.078
6.988IleSer: 6.988 ± 0.139
4.356IleThr: 4.356 ± 0.119
5.423IleVal: 5.423 ± 0.106
0.446IleTrp: 0.446 ± 0.033
3.954IleTyr: 3.954 ± 0.094
0.0IleXaa: 0.0 ± 0.0
Lys
4.605LysAla: 4.605 ± 0.117
0.649LysCys: 0.649 ± 0.043
6.525LysAsp: 6.525 ± 0.14
8.694LysGlu: 8.694 ± 0.176
4.481LysPhe: 4.481 ± 0.099
5.062LysGly: 5.062 ± 0.128
1.344LysHis: 1.344 ± 0.057
11.014LysIle: 11.014 ± 0.188
9.229LysLys: 9.229 ± 0.176
8.743LysLeu: 8.743 ± 0.148
2.285LysMet: 2.285 ± 0.065
8.165LysAsn: 8.165 ± 0.153
1.772LysPro: 1.772 ± 0.078
2.869LysGln: 2.869 ± 0.105
3.573LysArg: 3.573 ± 0.09
6.748LysSer: 6.748 ± 0.156
4.846LysThr: 4.846 ± 0.117
5.551LysVal: 5.551 ± 0.125
0.524LysTrp: 0.524 ± 0.033
4.131LysTyr: 4.131 ± 0.122
0.0LysXaa: 0.0 ± 0.0
Leu
4.425LeuAla: 4.425 ± 0.1
0.794LeuCys: 0.794 ± 0.048
5.86LeuAsp: 5.86 ± 0.146
7.138LeuGlu: 7.138 ± 0.144
5.058LeuPhe: 5.058 ± 0.156
5.615LeuGly: 5.615 ± 0.141
1.317LeuHis: 1.317 ± 0.058
9.271LeuIle: 9.271 ± 0.198
11.554LeuLys: 11.554 ± 0.2
8.995LeuLeu: 8.995 ± 0.165
2.075LeuMet: 2.075 ± 0.063
7.653LeuAsn: 7.653 ± 0.152
2.238LeuPro: 2.238 ± 0.083
2.82LeuGln: 2.82 ± 0.084
3.975LeuArg: 3.975 ± 0.093
7.659LeuSer: 7.659 ± 0.132
3.972LeuThr: 3.972 ± 0.099
5.031LeuVal: 5.031 ± 0.121
0.392LeuTrp: 0.392 ± 0.027
3.27LeuTyr: 3.27 ± 0.085
0.0LeuXaa: 0.0 ± 0.0
Met
1.066MetAla: 1.066 ± 0.048
0.149MetCys: 0.149 ± 0.02
1.008MetAsp: 1.008 ± 0.052
1.239MetGlu: 1.239 ± 0.056
1.09MetPhe: 1.09 ± 0.054
1.42MetGly: 1.42 ± 0.064
0.441MetHis: 0.441 ± 0.035
2.084MetIle: 2.084 ± 0.067
2.238MetLys: 2.238 ± 0.074
2.225MetLeu: 2.225 ± 0.073
1.092MetMet: 1.092 ± 0.066
1.407MetAsn: 1.407 ± 0.061
0.557MetPro: 0.557 ± 0.037
0.925MetGln: 0.925 ± 0.039
0.945MetArg: 0.945 ± 0.048
1.587MetSer: 1.587 ± 0.051
0.678MetThr: 0.678 ± 0.039
1.429MetVal: 1.429 ± 0.065
0.089MetTrp: 0.089 ± 0.016
0.667MetTyr: 0.667 ± 0.043
0.0MetXaa: 0.0 ± 0.0
Asn
3.578AsnAla: 3.578 ± 0.106
0.502AsnCys: 0.502 ± 0.033
3.687AsnAsp: 3.687 ± 0.106
4.621AsnGlu: 4.621 ± 0.122
4.122AsnPhe: 4.122 ± 0.109
3.114AsnGly: 3.114 ± 0.079
0.798AsnHis: 0.798 ± 0.046
7.699AsnIle: 7.699 ± 0.163
7.59AsnLys: 7.59 ± 0.177
7.873AsnLeu: 7.873 ± 0.166
1.527AsnMet: 1.527 ± 0.053
5.878AsnAsn: 5.878 ± 0.178
1.877AsnPro: 1.877 ± 0.062
1.899AsnGln: 1.899 ± 0.086
2.249AsnArg: 2.249 ± 0.079
5.031AsnSer: 5.031 ± 0.131
3.177AsnThr: 3.177 ± 0.096
4.041AsnVal: 4.041 ± 0.112
0.328AsnTrp: 0.328 ± 0.026
3.038AsnTyr: 3.038 ± 0.099
0.0AsnXaa: 0.0 ± 0.0
Pro
0.936ProAla: 0.936 ± 0.063
0.132ProCys: 0.132 ± 0.019
1.119ProAsp: 1.119 ± 0.068
1.398ProGlu: 1.398 ± 0.073
1.395ProPhe: 1.395 ± 0.063
1.061ProGly: 1.061 ± 0.055
0.428ProHis: 0.428 ± 0.034
2.465ProIle: 2.465 ± 0.088
2.127ProLys: 2.127 ± 0.072
1.984ProLeu: 1.984 ± 0.07
0.45ProMet: 0.45 ± 0.029
1.525ProAsn: 1.525 ± 0.057
0.477ProPro: 0.477 ± 0.033
0.622ProGln: 0.622 ± 0.034
0.604ProArg: 0.604 ± 0.043
1.632ProSer: 1.632 ± 0.066
1.119ProThr: 1.119 ± 0.053
1.282ProVal: 1.282 ± 0.059
0.176ProTrp: 0.176 ± 0.02
0.961ProTyr: 0.961 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
1.331GlnAla: 1.331 ± 0.059
0.129GlnCys: 0.129 ± 0.017
1.681GlnAsp: 1.681 ± 0.065
2.062GlnGlu: 2.062 ± 0.083
1.11GlnPhe: 1.11 ± 0.056
1.453GlnGly: 1.453 ± 0.06
0.341GlnHis: 0.341 ± 0.033
2.697GlnIle: 2.697 ± 0.077
3.156GlnLys: 3.156 ± 0.107
2.008GlnLeu: 2.008 ± 0.065
0.678GlnMet: 0.678 ± 0.044
2.421GlnAsn: 2.421 ± 0.082
0.412GlnPro: 0.412 ± 0.035
0.883GlnGln: 0.883 ± 0.052
1.061GlnArg: 1.061 ± 0.057
1.618GlnSer: 1.618 ± 0.06
1.21GlnThr: 1.21 ± 0.059
1.5GlnVal: 1.5 ± 0.053
0.127GlnTrp: 0.127 ± 0.018
0.992GlnTyr: 0.992 ± 0.048
0.0GlnXaa: 0.0 ± 0.0
Arg
1.734ArgAla: 1.734 ± 0.078
0.196ArgCys: 0.196 ± 0.021
2.249ArgAsp: 2.249 ± 0.088
3.344ArgGlu: 3.344 ± 0.118
1.752ArgPhe: 1.752 ± 0.062
1.835ArgGly: 1.835 ± 0.076
0.544ArgHis: 0.544 ± 0.033
3.19ArgIle: 3.19 ± 0.076
2.882ArgLys: 2.882 ± 0.078
3.032ArgLeu: 3.032 ± 0.084
0.883ArgMet: 0.883 ± 0.043
2.214ArgAsn: 2.214 ± 0.073
0.74ArgPro: 0.74 ± 0.049
0.961ArgGln: 0.961 ± 0.051
1.567ArgArg: 1.567 ± 0.064
1.926ArgSer: 1.926 ± 0.066
1.382ArgThr: 1.382 ± 0.057
2.423ArgVal: 2.423 ± 0.085
0.163ArgTrp: 0.163 ± 0.02
1.507ArgTyr: 1.507 ± 0.068
0.0ArgXaa: 0.0 ± 0.0
Ser
3.15SerAla: 3.15 ± 0.102
0.753SerCys: 0.753 ± 0.038
3.825SerAsp: 3.825 ± 0.103
4.224SerGlu: 4.224 ± 0.106
4.017SerPhe: 4.017 ± 0.116
4.108SerGly: 4.108 ± 0.088
0.967SerHis: 0.967 ± 0.046
6.743SerIle: 6.743 ± 0.151
6.832SerLys: 6.832 ± 0.122
7.664SerLeu: 7.664 ± 0.159
1.331SerMet: 1.331 ± 0.056
4.543SerAsn: 4.543 ± 0.114
1.362SerPro: 1.362 ± 0.064
1.866SerGln: 1.866 ± 0.067
2.387SerArg: 2.387 ± 0.084
5.129SerSer: 5.129 ± 0.133
2.76SerThr: 2.76 ± 0.082
4.093SerVal: 4.093 ± 0.104
0.348SerTrp: 0.348 ± 0.03
3.025SerTyr: 3.025 ± 0.076
0.0SerXaa: 0.0 ± 0.0
Thr
2.18ThrAla: 2.18 ± 0.086
0.321ThrCys: 0.321 ± 0.027
1.919ThrAsp: 1.919 ± 0.066
2.414ThrGlu: 2.414 ± 0.074
2.158ThrPhe: 2.158 ± 0.074
2.514ThrGly: 2.514 ± 0.087
0.702ThrHis: 0.702 ± 0.041
4.09ThrIle: 4.09 ± 0.106
4.42ThrLys: 4.42 ± 0.125
5.045ThrLeu: 5.045 ± 0.099
0.816ThrMet: 0.816 ± 0.042
3.0ThrAsn: 3.0 ± 0.081
1.38ThrPro: 1.38 ± 0.055
1.337ThrGln: 1.337 ± 0.06
1.389ThrArg: 1.389 ± 0.055
3.123ThrSer: 3.123 ± 0.086
2.28ThrThr: 2.28 ± 0.087
2.247ThrVal: 2.247 ± 0.078
0.198ThrTrp: 0.198 ± 0.023
1.806ThrTyr: 1.806 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
3.185ValAla: 3.185 ± 0.093
0.444ValCys: 0.444 ± 0.032
3.667ValAsp: 3.667 ± 0.095
3.732ValGlu: 3.732 ± 0.112
3.201ValPhe: 3.201 ± 0.087
3.72ValGly: 3.72 ± 0.112
0.655ValHis: 0.655 ± 0.037
4.917ValIle: 4.917 ± 0.12
5.943ValLys: 5.943 ± 0.135
5.945ValLeu: 5.945 ± 0.122
1.431ValMet: 1.431 ± 0.061
4.144ValAsn: 4.144 ± 0.113
1.382ValPro: 1.382 ± 0.061
1.3ValGln: 1.3 ± 0.05
1.904ValArg: 1.904 ± 0.065
4.251ValSer: 4.251 ± 0.101
2.214ValThr: 2.214 ± 0.078
3.868ValVal: 3.868 ± 0.107
0.243ValTrp: 0.243 ± 0.025
2.185ValTyr: 2.185 ± 0.073
0.0ValXaa: 0.0 ± 0.0
Trp
0.252TrpAla: 0.252 ± 0.025
0.076TrpCys: 0.076 ± 0.014
0.283TrpAsp: 0.283 ± 0.027
0.314TrpGlu: 0.314 ± 0.029
0.239TrpPhe: 0.239 ± 0.023
0.296TrpGly: 0.296 ± 0.025
0.078TrpHis: 0.078 ± 0.012
0.51TrpIle: 0.51 ± 0.035
0.33TrpLys: 0.33 ± 0.029
0.395TrpLeu: 0.395 ± 0.029
0.181TrpMet: 0.181 ± 0.017
0.415TrpAsn: 0.415 ± 0.031
0.116TrpPro: 0.116 ± 0.016
0.16TrpGln: 0.16 ± 0.02
0.172TrpArg: 0.172 ± 0.019
0.279TrpSer: 0.279 ± 0.023
0.198TrpThr: 0.198 ± 0.022
0.203TrpVal: 0.203 ± 0.024
0.036TrpTrp: 0.036 ± 0.009
0.216TrpTyr: 0.216 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.705TyrAla: 1.705 ± 0.057
0.366TyrCys: 0.366 ± 0.03
2.51TyrAsp: 2.51 ± 0.095
2.63TyrGlu: 2.63 ± 0.085
2.871TyrPhe: 2.871 ± 0.081
1.835TyrGly: 1.835 ± 0.072
0.624TyrHis: 0.624 ± 0.034
3.573TyrIle: 3.573 ± 0.09
4.162TyrLys: 4.162 ± 0.099
4.532TyrLeu: 4.532 ± 0.106
0.809TyrMet: 0.809 ± 0.047
2.829TyrAsn: 2.829 ± 0.086
1.021TyrPro: 1.021 ± 0.048
0.938TyrGln: 0.938 ± 0.049
1.382TyrArg: 1.382 ± 0.058
3.203TyrSer: 3.203 ± 0.087
1.587TyrThr: 1.587 ± 0.062
2.051TyrVal: 2.051 ± 0.065
0.267TyrTrp: 0.267 ± 0.023
2.448TyrTyr: 2.448 ± 0.093
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1995 proteins (448606 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski