Candidatus Walczuchella monophlebidarum
Average proteome isoelectric point is 8.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 243 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068DNU0|A0A068DNU0_9FLAO GTPase Obg OS=Candidatus Walczuchella monophlebidarum OX=1415657 GN=obg PE=3 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 3.98 LASYY10 pKa = 10.9 LEE12 pKa = 4.44 DD13 pKa = 4.82 APWPANKK20 pKa = 10.23 DD21 pKa = 3.59 EE22 pKa = 5.39 LIDD25 pKa = 3.49 YY26 pKa = 10.37 AMRR29 pKa = 11.84 SGAPLAVIEE38 pKa = 4.53 NLQQLEE44 pKa = 4.38 DD45 pKa = 4.67 DD46 pKa = 4.01 GDD48 pKa = 3.96 TYY50 pKa = 11.66 EE51 pKa = 4.9 SIEE54 pKa = 4.85 EE55 pKa = 3.8 IWIDD59 pKa = 3.65 YY60 pKa = 7.36 PTNEE64 pKa = 4.44 DD65 pKa = 3.74 LFFNEE70 pKa = 5.67 DD71 pKa = 3.37 EE72 pKa = 4.76 FSGKK76 pKa = 9.2 NPKK79 pKa = 10.05 KK80 pKa = 10.21 FLRR83 pKa = 11.84 KK84 pKa = 9.49
Molecular weight: 9.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.186
IPC2_protein 3.961
IPC_protein 3.884
Toseland 3.694
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.617
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.77
Sillero 3.999
Patrickios 3.427
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.924
Protein with the highest isoelectric point:
>tr|A0A068DQB7|A0A068DQB7_9FLAO DNA-directed RNA polymerase subunit omega OS=Candidatus Walczuchella monophlebidarum OX=1415657 GN=FNIIJ_133 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.41 RR3 pKa = 11.84 TYY5 pKa = 10.21 QPSGRR10 pKa = 11.84 KK11 pKa = 8.78 RR12 pKa = 11.84 RR13 pKa = 11.84 NVHH16 pKa = 5.8 GFIKK20 pKa = 10.61 RR21 pKa = 11.84 MGTKK25 pKa = 9.99 GGRR28 pKa = 11.84 KK29 pKa = 8.08 ILSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 8.37 NLTVSNVRR48 pKa = 11.84 YY49 pKa = 9.88 KK50 pKa = 10.75 RR51 pKa = 3.46
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 11.082
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.252
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.974
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.022
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
243
0
243
82362
39
1417
338.9
38.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.6 ± 0.104
1.121 ± 0.047
4.429 ± 0.081
6.664 ± 0.144
4.866 ± 0.139
6.125 ± 0.128
2.249 ± 0.06
10.177 ± 0.146
9.462 ± 0.155
9.416 ± 0.126
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.127 ± 0.047
5.439 ± 0.089
3.465 ± 0.066
3.363 ± 0.061
4.701 ± 0.09
6.893 ± 0.097
4.654 ± 0.078
5.282 ± 0.111
0.93 ± 0.065
4.036 ± 0.092
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here