Desulfobulbus sp. Tol-SR

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Desulfobulbus; unclassified Desulfobulbus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2HW43|A0A0A2HW43_9DELT C4-dicarboxylate ABC transporter OS=Desulfobulbus sp. Tol-SR OX=1536652 GN=JT06_04610 PE=4 SV=1
MM1 pKa = 7.17TMNRR5 pKa = 11.84LLMAILALGLVLFTADD21 pKa = 3.71LQAGEE26 pKa = 4.13HH27 pKa = 6.6RR28 pKa = 11.84IGGGANYY35 pKa = 8.62WVAVDD40 pKa = 5.27DD41 pKa = 4.48LDD43 pKa = 5.07EE44 pKa = 5.13EE45 pKa = 5.34GVDD48 pKa = 4.01DD49 pKa = 6.61DD50 pKa = 6.41DD51 pKa = 4.68GFSYY55 pKa = 10.53LVSYY59 pKa = 7.15QHH61 pKa = 6.04WWDD64 pKa = 3.54FLGIEE69 pKa = 4.51VDD71 pKa = 4.25MEE73 pKa = 4.32LLPDD77 pKa = 3.97RR78 pKa = 11.84FGEE81 pKa = 4.0TAYY84 pKa = 10.65APEE87 pKa = 5.07AYY89 pKa = 9.16ILVGSTIYY97 pKa = 10.77AGAGMGLIRR106 pKa = 11.84TDD108 pKa = 3.6GDD110 pKa = 3.6FADD113 pKa = 4.24DD114 pKa = 3.47PFYY117 pKa = 11.34AFRR120 pKa = 11.84VGLNLEE126 pKa = 4.44MLPSVYY132 pKa = 10.52VDD134 pKa = 3.86LSANYY139 pKa = 9.71RR140 pKa = 11.84FNDD143 pKa = 3.54SAQLEE148 pKa = 4.4DD149 pKa = 5.46DD150 pKa = 4.45DD151 pKa = 5.21TDD153 pKa = 3.77IDD155 pKa = 3.76TDD157 pKa = 4.12TIFFGAALRR166 pKa = 11.84FALL169 pKa = 4.85

Molecular weight: 18.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2HXD9|A0A0A2HXD9_9DELT Uncharacterized protein OS=Desulfobulbus sp. Tol-SR OX=1536652 GN=JT06_06955 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.3DD3 pKa = 3.32SLPPRR8 pKa = 11.84LARR11 pKa = 11.84EE12 pKa = 4.02LATITAMIGIHH23 pKa = 6.45CRR25 pKa = 11.84DD26 pKa = 3.27HH27 pKa = 6.5HH28 pKa = 7.5HH29 pKa = 7.01GEE31 pKa = 4.1GRR33 pKa = 11.84QLCPQCRR40 pKa = 11.84EE41 pKa = 3.76LAAYY45 pKa = 8.12AAKK48 pKa = 10.43RR49 pKa = 11.84LAKK52 pKa = 10.58CPFQQKK58 pKa = 10.18KK59 pKa = 6.78PTCGNCPVHH68 pKa = 6.97CYY70 pKa = 10.1APRR73 pKa = 11.84YY74 pKa = 8.32RR75 pKa = 11.84IEE77 pKa = 3.84VRR79 pKa = 11.84SVMRR83 pKa = 11.84YY84 pKa = 9.4AGSRR88 pKa = 11.84MIWAHH93 pKa = 6.23PLMALRR99 pKa = 11.84HH100 pKa = 5.6LLDD103 pKa = 3.41GRR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84APVAGQRR114 pKa = 11.84HH115 pKa = 5.98HH116 pKa = 6.91NGLSIRR122 pKa = 11.84DD123 pKa = 3.8KK124 pKa = 11.41NRR126 pKa = 11.84TKK128 pKa = 10.67PP129 pKa = 3.3

Molecular weight: 14.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3201

0

3201

984164

34

2051

307.5

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.543 ± 0.046

1.352 ± 0.02

5.548 ± 0.033

6.264 ± 0.04

4.032 ± 0.03

7.965 ± 0.041

2.178 ± 0.019

6.405 ± 0.033

4.342 ± 0.043

10.369 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.02

3.062 ± 0.026

4.586 ± 0.029

3.467 ± 0.028

6.756 ± 0.047

5.347 ± 0.027

5.219 ± 0.029

7.114 ± 0.033

1.102 ± 0.017

2.723 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski