Trichechus manatus papillomavirus 3
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6TNZ5|A0A0F6TNZ5_9PAPI Replication protein E1 OS=Trichechus manatus papillomavirus 3 OX=1429799 GN=E1 PE=3 SV=1
MM1 pKa = 7.49 PRR3 pKa = 11.84 LRR5 pKa = 11.84 TKK7 pKa = 10.47 RR8 pKa = 11.84 ATVEE12 pKa = 4.0 SIYY15 pKa = 10.46 RR16 pKa = 11.84 SCKK19 pKa = 8.62 ATNTCSADD27 pKa = 3.44 VINTVEE33 pKa = 4.05 QNTLADD39 pKa = 5.17 RR40 pKa = 11.84 LLKK43 pKa = 9.91 WISGLVYY50 pKa = 10.41 FGNLGIGTAGGGGGRR65 pKa = 11.84 FGYY68 pKa = 10.16 GALGGTRR75 pKa = 11.84 AAITPSAARR84 pKa = 11.84 PPVVPEE90 pKa = 3.71 TLGLLEE96 pKa = 4.28 VAGPTAGEE104 pKa = 3.89 IDD106 pKa = 3.73 AATPSIVPLLDD117 pKa = 3.57 GSTVEE122 pKa = 4.34 SVNIEE127 pKa = 3.95 AVAEE131 pKa = 3.99 VHH133 pKa = 6.37 PAPPAQGTSTLGGVAEE149 pKa = 4.52 TTGSILPASDD159 pKa = 4.52 PPIQTAVTHH168 pKa = 6.0 TEE170 pKa = 4.17 FSNPAYY176 pKa = 9.96 EE177 pKa = 4.42 ITTSTSDD184 pKa = 3.34 SVGEE188 pKa = 4.23 ISTGDD193 pKa = 3.27 HH194 pKa = 5.24 MVVVGGGSGQSIGEE208 pKa = 4.47 EE209 pKa = 3.81 IPLLQLSPDD218 pKa = 3.53 NTFDD222 pKa = 3.41 TSDD225 pKa = 3.44 VLEE228 pKa = 4.42 TEE230 pKa = 4.13 FGGRR234 pKa = 11.84 TSTPDD239 pKa = 3.04 TQAPRR244 pKa = 11.84 VRR246 pKa = 11.84 GDD248 pKa = 2.87 RR249 pKa = 11.84 GLLYY253 pKa = 10.63 SRR255 pKa = 11.84 YY256 pKa = 5.88 TTQQEE261 pKa = 3.9 ITNPTFLSTPYY272 pKa = 11.13 DD273 pKa = 3.3 LVAYY277 pKa = 8.53 NNPAFEE283 pKa = 4.64 GTDD286 pKa = 3.31 SFDD289 pKa = 3.96 FPPAASPYY297 pKa = 8.38 TVEE300 pKa = 4.62 AAPDD304 pKa = 3.6 PAFNDD309 pKa = 3.56 ILHH312 pKa = 6.99 LGHH315 pKa = 6.96 ARR317 pKa = 11.84 YY318 pKa = 8.07 STTATNRR325 pKa = 11.84 VRR327 pKa = 11.84 VSRR330 pKa = 11.84 LGTRR334 pKa = 11.84 PGVRR338 pKa = 11.84 TRR340 pKa = 11.84 TGTLLTGRR348 pKa = 11.84 THH350 pKa = 7.96 FYY352 pKa = 10.99 QDD354 pKa = 3.25 LSSINEE360 pKa = 4.21 SLEE363 pKa = 3.81 LTPLSEE369 pKa = 5.58 RR370 pKa = 11.84 IPSSHH375 pKa = 6.15 STSTSIGQSIQGYY388 pKa = 8.61 PDD390 pKa = 3.87 NDD392 pKa = 4.61 PIIIDD397 pKa = 3.68 SSFEE401 pKa = 4.32 IIDD404 pKa = 4.08 LNSTSAEE411 pKa = 3.95 YY412 pKa = 10.95 SEE414 pKa = 6.05 ADD416 pKa = 3.69 LLDD419 pKa = 3.88 IYY421 pKa = 11.28 EE422 pKa = 5.56 DD423 pKa = 3.8 IADD426 pKa = 4.01 HH427 pKa = 6.04 AQLVIGRR434 pKa = 11.84 PRR436 pKa = 11.84 HH437 pKa = 4.9 PTTVNIPIEE446 pKa = 4.02 NTIGKK451 pKa = 8.25 DD452 pKa = 3.39 TIIDD456 pKa = 3.65 SSSGFFVEE464 pKa = 4.51 VPEE467 pKa = 4.45 VHH469 pKa = 6.11 GTTLNPTQTDD479 pKa = 3.45 RR480 pKa = 11.84 DD481 pKa = 3.56 GHH483 pKa = 7.04 IFVYY487 pKa = 10.42 SPHH490 pKa = 6.11 LTSFDD495 pKa = 4.08 FLPHH499 pKa = 6.59 PSLLKK504 pKa = 10.22 RR505 pKa = 11.84 KK506 pKa = 9.5 RR507 pKa = 11.84 KK508 pKa = 9.65 RR509 pKa = 11.84 SLDD512 pKa = 3.48 DD513 pKa = 4.98 DD514 pKa = 4.02 FTILQQ519 pKa = 3.8
Molecular weight: 55.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.661
IPC2_protein 4.66
IPC_protein 4.622
Toseland 4.482
ProMoST 4.749
Dawson 4.596
Bjellqvist 4.736
Wikipedia 4.482
Rodwell 4.482
Grimsley 4.406
Solomon 4.596
Lehninger 4.546
Nozaki 4.711
DTASelect 4.889
Thurlkill 4.495
EMBOSS 4.507
Sillero 4.762
Patrickios 3.821
IPC_peptide 4.596
IPC2_peptide 4.749
IPC2.peptide.svr19 4.685
Protein with the highest isoelectric point:
>tr|A0A0F6RB75|A0A0F6RB75_9PAPI Regulatory protein E2 OS=Trichechus manatus papillomavirus 3 OX=1429799 GN=E2 PE=3 SV=1
MM1 pKa = 7.51 SLPRR5 pKa = 11.84 TLQSLRR11 pKa = 11.84 DD12 pKa = 4.11 DD13 pKa = 4.24 FGLDD17 pKa = 3.12 YY18 pKa = 8.47 TTFYY22 pKa = 9.74 IACVLCGGKK31 pKa = 10.26 LGTLDD36 pKa = 4.43 LMNFEE41 pKa = 4.8 KK42 pKa = 11.1 GRR44 pKa = 11.84 FFLIWQYY51 pKa = 11.29 GLPWACCKK59 pKa = 10.56 FCIRR63 pKa = 11.84 WWAAKK68 pKa = 10.3 DD69 pKa = 3.42 RR70 pKa = 11.84 VANYY74 pKa = 9.93 QYY76 pKa = 10.18 SAPVATVEE84 pKa = 4.88 AEE86 pKa = 4.43 TKK88 pKa = 8.63 QTLAEE93 pKa = 4.0 LHH95 pKa = 5.97 VRR97 pKa = 11.84 CIGCLTEE104 pKa = 4.66 LTYY107 pKa = 10.87 LDD109 pKa = 3.59 KK110 pKa = 11.35 LRR112 pKa = 11.84 IEE114 pKa = 4.26 RR115 pKa = 11.84 LGACLHH121 pKa = 6.37 LCRR124 pKa = 11.84 NKK126 pKa = 9.38 WRR128 pKa = 11.84 GWCSRR133 pKa = 11.84 CQRR136 pKa = 11.84 QWW138 pKa = 2.67
Molecular weight: 16.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.041
IPC2_protein 7.922
IPC_protein 7.732
Toseland 7.146
ProMoST 8.302
Dawson 8.419
Bjellqvist 8.916
Wikipedia 8.317
Rodwell 8.434
Grimsley 7.088
Solomon 8.492
Lehninger 8.521
Nozaki 9.224
DTASelect 8.551
Thurlkill 8.595
EMBOSS 8.624
Sillero 8.975
Patrickios 3.681
IPC_peptide 8.492
IPC2_peptide 8.404
IPC2.peptide.svr19 8.553
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2337
99
628
389.5
43.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.033 ± 0.461
2.525 ± 1.092
6.376 ± 0.367
5.991 ± 0.468
3.894 ± 0.418
6.675 ± 0.796
2.182 ± 0.143
4.707 ± 0.606
4.108 ± 0.801
8.772 ± 0.643
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.369 ± 0.328
3.894 ± 0.553
6.675 ± 1.083
4.279 ± 0.594
6.59 ± 0.57
7.274 ± 0.818
7.745 ± 1.183
5.777 ± 0.33
1.669 ± 0.574
3.466 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here