Amino acid dipepetide frequency for Vibrio phage PWH3a-P1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.662AlaAla: 2.662 ± 0.347
0.78AlaCys: 0.78 ± 0.147
2.34AlaAsp: 2.34 ± 0.264
2.582AlaGlu: 2.582 ± 0.27
2.017AlaPhe: 2.017 ± 0.251
2.851AlaGly: 2.851 ± 0.3
0.834AlaHis: 0.834 ± 0.178
2.958AlaIle: 2.958 ± 0.337
3.738AlaLys: 3.738 ± 0.295
3.577AlaLeu: 3.577 ± 0.301
1.156AlaMet: 1.156 ± 0.201
1.99AlaAsn: 1.99 ± 0.311
1.264AlaPro: 1.264 ± 0.179
1.856AlaGln: 1.856 ± 0.23
1.64AlaArg: 1.64 ± 0.197
2.958AlaSer: 2.958 ± 0.302
2.662AlaThr: 2.662 ± 0.393
2.77AlaVal: 2.77 ± 0.333
0.484AlaTrp: 0.484 ± 0.118
1.721AlaTyr: 1.721 ± 0.212
0.0AlaXaa: 0.0 ± 0.0
Cys
0.619CysAla: 0.619 ± 0.121
0.592CysCys: 0.592 ± 0.134
1.076CysAsp: 1.076 ± 0.194
1.694CysGlu: 1.694 ± 0.22
0.565CysPhe: 0.565 ± 0.114
1.829CysGly: 1.829 ± 0.233
0.296CysHis: 0.296 ± 0.089
1.049CysIle: 1.049 ± 0.189
1.721CysLys: 1.721 ± 0.245
1.398CysLeu: 1.398 ± 0.194
0.457CysMet: 0.457 ± 0.1
0.968CysAsn: 0.968 ± 0.176
0.565CysPro: 0.565 ± 0.112
0.484CysGln: 0.484 ± 0.12
0.592CysArg: 0.592 ± 0.119
2.259CysSer: 2.259 ± 0.355
0.699CysThr: 0.699 ± 0.143
0.941CysVal: 0.941 ± 0.19
0.35CysTrp: 0.35 ± 0.108
0.753CysTyr: 0.753 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
2.904AspAla: 2.904 ± 0.297
1.21AspCys: 1.21 ± 0.199
3.657AspAsp: 3.657 ± 0.308
5.782AspGlu: 5.782 ± 0.476
3.281AspPhe: 3.281 ± 0.323
4.599AspGly: 4.599 ± 0.36
1.156AspHis: 1.156 ± 0.165
4.303AspIle: 4.303 ± 0.372
5.351AspLys: 5.351 ± 0.347
5.351AspLeu: 5.351 ± 0.366
1.587AspMet: 1.587 ± 0.199
4.41AspAsn: 4.41 ± 0.365
1.963AspPro: 1.963 ± 0.208
1.694AspGln: 1.694 ± 0.193
2.232AspArg: 2.232 ± 0.246
4.383AspSer: 4.383 ± 0.389
4.088AspThr: 4.088 ± 0.327
5.325AspVal: 5.325 ± 0.374
1.264AspTrp: 1.264 ± 0.198
3.765AspTyr: 3.765 ± 0.315
0.0AspXaa: 0.0 ± 0.0
Glu
4.061GluAla: 4.061 ± 0.418
1.425GluCys: 1.425 ± 0.214
6.051GluAsp: 6.051 ± 0.38
6.535GluGlu: 6.535 ± 0.483
3.469GluPhe: 3.469 ± 0.336
4.975GluGly: 4.975 ± 0.364
1.614GluHis: 1.614 ± 0.232
4.921GluIle: 4.921 ± 0.32
5.755GluLys: 5.755 ± 0.409
6.346GluLeu: 6.346 ± 0.378
2.205GluMet: 2.205 ± 0.263
4.356GluAsn: 4.356 ± 0.357
1.345GluPro: 1.345 ± 0.203
2.635GluGln: 2.635 ± 0.271
2.501GluArg: 2.501 ± 0.248
4.33GluSer: 4.33 ± 0.315
4.437GluThr: 4.437 ± 0.316
4.652GluVal: 4.652 ± 0.351
1.452GluTrp: 1.452 ± 0.208
4.464GluTyr: 4.464 ± 0.372
0.0GluXaa: 0.0 ± 0.0
Phe
1.775PheAla: 1.775 ± 0.252
0.699PheCys: 0.699 ± 0.149
3.308PheAsp: 3.308 ± 0.316
3.577PheGlu: 3.577 ± 0.27
1.291PhePhe: 1.291 ± 0.175
2.366PheGly: 2.366 ± 0.234
1.076PheHis: 1.076 ± 0.171
2.824PheIle: 2.824 ± 0.285
3.765PheLys: 3.765 ± 0.341
2.985PheLeu: 2.985 ± 0.311
1.129PheMet: 1.129 ± 0.144
3.254PheAsn: 3.254 ± 0.282
1.533PhePro: 1.533 ± 0.207
1.103PheGln: 1.103 ± 0.152
1.614PheArg: 1.614 ± 0.25
2.824PheSer: 2.824 ± 0.264
3.012PheThr: 3.012 ± 0.291
3.415PheVal: 3.415 ± 0.27
0.672PheTrp: 0.672 ± 0.12
2.151PheTyr: 2.151 ± 0.221
0.0PheXaa: 0.0 ± 0.0
Gly
2.178GlyAla: 2.178 ± 0.271
1.318GlyCys: 1.318 ± 0.209
4.383GlyAsp: 4.383 ± 0.371
4.276GlyGlu: 4.276 ± 0.294
3.066GlyPhe: 3.066 ± 0.303
3.98GlyGly: 3.98 ± 0.357
0.726GlyHis: 0.726 ± 0.157
3.765GlyIle: 3.765 ± 0.299
5.109GlyLys: 5.109 ± 0.42
5.19GlyLeu: 5.19 ± 0.374
1.506GlyMet: 1.506 ± 0.204
3.388GlyAsn: 3.388 ± 0.309
0.538GlyPro: 0.538 ± 0.119
1.587GlyGln: 1.587 ± 0.25
2.124GlyArg: 2.124 ± 0.191
4.034GlySer: 4.034 ± 0.294
2.366GlyThr: 2.366 ± 0.28
4.545GlyVal: 4.545 ± 0.378
1.345GlyTrp: 1.345 ± 0.172
3.926GlyTyr: 3.926 ± 0.329
0.0GlyXaa: 0.0 ± 0.0
His
0.78HisAla: 0.78 ± 0.14
0.43HisCys: 0.43 ± 0.109
1.103HisAsp: 1.103 ± 0.179
1.264HisGlu: 1.264 ± 0.204
0.78HisPhe: 0.78 ± 0.131
0.834HisGly: 0.834 ± 0.152
0.376HisHis: 0.376 ± 0.097
1.291HisIle: 1.291 ± 0.178
1.909HisLys: 1.909 ± 0.189
1.56HisLeu: 1.56 ± 0.213
0.43HisMet: 0.43 ± 0.113
1.425HisAsn: 1.425 ± 0.198
0.807HisPro: 0.807 ± 0.149
0.726HisGln: 0.726 ± 0.146
0.914HisArg: 0.914 ± 0.159
1.237HisSer: 1.237 ± 0.188
1.291HisThr: 1.291 ± 0.195
0.78HisVal: 0.78 ± 0.156
0.242HisTrp: 0.242 ± 0.089
1.049HisTyr: 1.049 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
3.119IleAla: 3.119 ± 0.331
0.834IleCys: 0.834 ± 0.158
4.679IleAsp: 4.679 ± 0.338
5.163IleGlu: 5.163 ± 0.375
2.501IlePhe: 2.501 ± 0.248
3.2IleGly: 3.2 ± 0.302
1.129IleHis: 1.129 ± 0.18
3.872IleIle: 3.872 ± 0.311
5.728IleLys: 5.728 ± 0.459
5.351IleLeu: 5.351 ± 0.388
1.667IleMet: 1.667 ± 0.208
4.061IleAsn: 4.061 ± 0.286
1.963IlePro: 1.963 ± 0.2
1.802IleGln: 1.802 ± 0.222
2.259IleArg: 2.259 ± 0.215
4.141IleSer: 4.141 ± 0.325
4.222IleThr: 4.222 ± 0.303
3.711IleVal: 3.711 ± 0.295
0.753IleTrp: 0.753 ± 0.158
2.286IleTyr: 2.286 ± 0.251
0.0IleXaa: 0.0 ± 0.0
Lys
3.388LysAla: 3.388 ± 0.358
1.291LysCys: 1.291 ± 0.176
6.4LysAsp: 6.4 ± 0.406
6.615LysGlu: 6.615 ± 0.455
3.684LysPhe: 3.684 ± 0.323
5.109LysGly: 5.109 ± 0.389
1.56LysHis: 1.56 ± 0.2
5.163LysIle: 5.163 ± 0.388
4.518LysLys: 4.518 ± 0.473
7.664LysLeu: 7.664 ± 0.468
2.528LysMet: 2.528 ± 0.281
4.249LysAsn: 4.249 ± 0.329
2.635LysPro: 2.635 ± 0.305
3.281LysGln: 3.281 ± 0.276
3.926LysArg: 3.926 ± 0.392
5.674LysSer: 5.674 ± 0.424
4.706LysThr: 4.706 ± 0.318
6.32LysVal: 6.32 ± 0.39
1.022LysTrp: 1.022 ± 0.158
3.711LysTyr: 3.711 ± 0.297
0.0LysXaa: 0.0 ± 0.0
Leu
4.249LeuAla: 4.249 ± 0.432
1.479LeuCys: 1.479 ± 0.212
6.75LeuAsp: 6.75 ± 0.456
6.158LeuGlu: 6.158 ± 0.373
3.146LeuPhe: 3.146 ± 0.323
4.841LeuGly: 4.841 ± 0.412
1.963LeuHis: 1.963 ± 0.237
4.545LeuIle: 4.545 ± 0.309
7.099LeuLys: 7.099 ± 0.449
6.266LeuLeu: 6.266 ± 0.38
2.098LeuMet: 2.098 ± 0.229
4.464LeuAsn: 4.464 ± 0.332
2.958LeuPro: 2.958 ± 0.291
2.797LeuGln: 2.797 ± 0.274
3.711LeuArg: 3.711 ± 0.287
6.642LeuSer: 6.642 ± 0.453
5.378LeuThr: 5.378 ± 0.297
4.464LeuVal: 4.464 ± 0.306
1.129LeuTrp: 1.129 ± 0.176
3.711LeuTyr: 3.711 ± 0.332
0.0LeuXaa: 0.0 ± 0.0
Met
1.129MetAla: 1.129 ± 0.159
0.43MetCys: 0.43 ± 0.102
1.614MetAsp: 1.614 ± 0.187
2.232MetGlu: 2.232 ± 0.227
1.345MetPhe: 1.345 ± 0.206
1.076MetGly: 1.076 ± 0.169
0.215MetHis: 0.215 ± 0.083
1.882MetIle: 1.882 ± 0.256
2.501MetLys: 2.501 ± 0.264
1.829MetLeu: 1.829 ± 0.211
0.484MetMet: 0.484 ± 0.133
1.237MetAsn: 1.237 ± 0.19
0.403MetPro: 0.403 ± 0.105
0.995MetGln: 0.995 ± 0.162
1.183MetArg: 1.183 ± 0.157
1.694MetSer: 1.694 ± 0.202
1.829MetThr: 1.829 ± 0.241
1.291MetVal: 1.291 ± 0.184
0.269MetTrp: 0.269 ± 0.09
0.753MetTyr: 0.753 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
1.829AsnAla: 1.829 ± 0.249
0.672AsnCys: 0.672 ± 0.166
3.146AsnAsp: 3.146 ± 0.295
3.2AsnGlu: 3.2 ± 0.349
2.635AsnPhe: 2.635 ± 0.221
3.496AsnGly: 3.496 ± 0.29
1.398AsnHis: 1.398 ± 0.167
3.899AsnIle: 3.899 ± 0.367
6.024AsnLys: 6.024 ± 0.421
5.54AsnLeu: 5.54 ± 0.381
1.56AsnMet: 1.56 ± 0.184
3.577AsnAsn: 3.577 ± 0.321
2.151AsnPro: 2.151 ± 0.239
2.071AsnGln: 2.071 ± 0.219
2.662AsnArg: 2.662 ± 0.269
3.254AsnSer: 3.254 ± 0.24
3.657AsnThr: 3.657 ± 0.334
3.308AsnVal: 3.308 ± 0.316
0.699AsnTrp: 0.699 ± 0.138
2.958AsnTyr: 2.958 ± 0.3
0.0AsnXaa: 0.0 ± 0.0
Pro
0.861ProAla: 0.861 ± 0.157
0.834ProCys: 0.834 ± 0.145
2.044ProAsp: 2.044 ± 0.242
3.119ProGlu: 3.119 ± 0.289
1.64ProPhe: 1.64 ± 0.213
0.108ProGly: 0.108 ± 0.054
0.592ProHis: 0.592 ± 0.128
1.64ProIle: 1.64 ± 0.207
2.017ProLys: 2.017 ± 0.216
2.555ProLeu: 2.555 ± 0.283
0.592ProMet: 0.592 ± 0.136
1.479ProAsn: 1.479 ± 0.191
0.619ProPro: 0.619 ± 0.13
1.049ProGln: 1.049 ± 0.185
0.914ProArg: 0.914 ± 0.189
2.555ProSer: 2.555 ± 0.237
1.694ProThr: 1.694 ± 0.187
2.124ProVal: 2.124 ± 0.252
0.43ProTrp: 0.43 ± 0.123
1.56ProTyr: 1.56 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
1.748GlnAla: 1.748 ± 0.257
0.565GlnCys: 0.565 ± 0.129
2.366GlnAsp: 2.366 ± 0.245
2.797GlnGlu: 2.797 ± 0.281
1.56GlnPhe: 1.56 ± 0.163
1.748GlnGly: 1.748 ± 0.194
0.78GlnHis: 0.78 ± 0.128
1.936GlnIle: 1.936 ± 0.205
2.447GlnLys: 2.447 ± 0.309
2.662GlnLeu: 2.662 ± 0.273
0.645GlnMet: 0.645 ± 0.114
1.829GlnAsn: 1.829 ± 0.231
1.049GlnPro: 1.049 ± 0.156
1.667GlnGln: 1.667 ± 0.22
1.909GlnArg: 1.909 ± 0.219
1.506GlnSer: 1.506 ± 0.197
2.151GlnThr: 2.151 ± 0.246
1.936GlnVal: 1.936 ± 0.237
0.834GlnTrp: 0.834 ± 0.177
1.614GlnTyr: 1.614 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
1.587ArgAla: 1.587 ± 0.182
0.968ArgCys: 0.968 ± 0.191
2.34ArgAsp: 2.34 ± 0.21
3.308ArgGlu: 3.308 ± 0.366
1.694ArgPhe: 1.694 ± 0.199
2.635ArgGly: 2.635 ± 0.274
0.619ArgHis: 0.619 ± 0.137
1.99ArgIle: 1.99 ± 0.263
3.711ArgLys: 3.711 ± 0.325
3.577ArgLeu: 3.577 ± 0.351
0.941ArgMet: 0.941 ± 0.144
2.447ArgAsn: 2.447 ± 0.222
0.672ArgPro: 0.672 ± 0.121
1.398ArgGln: 1.398 ± 0.203
1.856ArgArg: 1.856 ± 0.314
2.743ArgSer: 2.743 ± 0.25
2.044ArgThr: 2.044 ± 0.198
2.931ArgVal: 2.931 ± 0.23
0.78ArgTrp: 0.78 ± 0.145
1.56ArgTyr: 1.56 ± 0.197
0.0ArgXaa: 0.0 ± 0.0
Ser
2.635SerAla: 2.635 ± 0.258
1.129SerCys: 1.129 ± 0.188
4.249SerAsp: 4.249 ± 0.354
5.19SerGlu: 5.19 ± 0.367
3.281SerPhe: 3.281 ± 0.291
4.303SerGly: 4.303 ± 0.401
1.129SerHis: 1.129 ± 0.186
3.872SerIle: 3.872 ± 0.309
5.298SerLys: 5.298 ± 0.45
5.916SerLeu: 5.916 ± 0.379
1.076SerMet: 1.076 ± 0.152
3.872SerAsn: 3.872 ± 0.348
2.124SerPro: 2.124 ± 0.195
2.34SerGln: 2.34 ± 0.243
2.662SerArg: 2.662 ± 0.261
4.76SerSer: 4.76 ± 0.422
3.738SerThr: 3.738 ± 0.321
4.76SerVal: 4.76 ± 0.424
1.345SerTrp: 1.345 ± 0.174
3.2SerTyr: 3.2 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
2.582ThrAla: 2.582 ± 0.351
1.076ThrCys: 1.076 ± 0.182
3.684ThrAsp: 3.684 ± 0.337
4.356ThrGlu: 4.356 ± 0.354
2.985ThrPhe: 2.985 ± 0.298
4.114ThrGly: 4.114 ± 0.366
1.237ThrHis: 1.237 ± 0.176
3.98ThrIle: 3.98 ± 0.327
4.975ThrLys: 4.975 ± 0.372
5.809ThrLeu: 5.809 ± 0.418
0.995ThrMet: 0.995 ± 0.179
3.093ThrAsn: 3.093 ± 0.336
2.528ThrPro: 2.528 ± 0.302
2.34ThrGln: 2.34 ± 0.224
1.667ThrArg: 1.667 ± 0.19
3.496ThrSer: 3.496 ± 0.388
3.388ThrThr: 3.388 ± 0.348
3.792ThrVal: 3.792 ± 0.345
0.592ThrTrp: 0.592 ± 0.126
2.555ThrTyr: 2.555 ± 0.305
0.0ThrXaa: 0.0 ± 0.0
Val
2.635ValAla: 2.635 ± 0.314
1.614ValCys: 1.614 ± 0.215
4.975ValAsp: 4.975 ± 0.302
5.029ValGlu: 5.029 ± 0.373
2.609ValPhe: 2.609 ± 0.258
3.711ValGly: 3.711 ± 0.347
1.156ValHis: 1.156 ± 0.188
4.41ValIle: 4.41 ± 0.371
5.836ValLys: 5.836 ± 0.435
4.948ValLeu: 4.948 ± 0.322
1.775ValMet: 1.775 ± 0.202
4.061ValAsn: 4.061 ± 0.323
1.829ValPro: 1.829 ± 0.257
1.398ValGln: 1.398 ± 0.229
2.689ValArg: 2.689 ± 0.32
4.841ValSer: 4.841 ± 0.395
3.765ValThr: 3.765 ± 0.31
5.378ValVal: 5.378 ± 0.408
0.78ValTrp: 0.78 ± 0.145
2.851ValTyr: 2.851 ± 0.278
0.0ValXaa: 0.0 ± 0.0
Trp
0.403TrpAla: 0.403 ± 0.099
0.484TrpCys: 0.484 ± 0.117
1.103TrpAsp: 1.103 ± 0.142
1.318TrpGlu: 1.318 ± 0.177
0.887TrpPhe: 0.887 ± 0.156
0.861TrpGly: 0.861 ± 0.163
0.323TrpHis: 0.323 ± 0.095
0.457TrpIle: 0.457 ± 0.116
1.237TrpLys: 1.237 ± 0.185
1.614TrpLeu: 1.614 ± 0.195
0.484TrpMet: 0.484 ± 0.118
0.753TrpAsn: 0.753 ± 0.133
0.081TrpPro: 0.081 ± 0.045
0.699TrpGln: 0.699 ± 0.103
0.726TrpArg: 0.726 ± 0.166
0.834TrpSer: 0.834 ± 0.139
0.807TrpThr: 0.807 ± 0.159
1.076TrpVal: 1.076 ± 0.179
0.511TrpTrp: 0.511 ± 0.12
0.914TrpTyr: 0.914 ± 0.17
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.694TyrAla: 1.694 ± 0.197
1.076TyrCys: 1.076 ± 0.171
3.039TyrAsp: 3.039 ± 0.309
3.335TyrGlu: 3.335 ± 0.352
1.936TyrPhe: 1.936 ± 0.2
2.716TyrGly: 2.716 ± 0.221
1.049TyrHis: 1.049 ± 0.19
3.684TyrIle: 3.684 ± 0.348
4.706TyrLys: 4.706 ± 0.438
3.711TyrLeu: 3.711 ± 0.346
1.049TyrMet: 1.049 ± 0.161
2.851TyrAsn: 2.851 ± 0.308
1.398TyrPro: 1.398 ± 0.194
1.829TyrGln: 1.829 ± 0.214
2.017TyrArg: 2.017 ± 0.222
2.716TyrSer: 2.716 ± 0.249
3.308TyrThr: 3.308 ± 0.276
2.77TyrVal: 2.77 ± 0.289
0.619TyrTrp: 0.619 ± 0.12
2.151TyrTyr: 2.151 ± 0.233
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 209 proteins (37187 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski