Microbacterium phage Theresita

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6E595|A0A4D6E595_9CAUD Lysin A OS=Microbacterium phage Theresita OX=2562367 GN=24 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 3.65MQSIRR7 pKa = 11.84EE8 pKa = 4.11AVEE11 pKa = 3.82GLGLVAYY18 pKa = 8.76TGYY21 pKa = 10.0PSSNSRR27 pKa = 11.84APYY30 pKa = 9.1VVVRR34 pKa = 11.84PMIVDD39 pKa = 3.95TEE41 pKa = 4.28NIAINGATIDD51 pKa = 3.43WDD53 pKa = 3.77HH54 pKa = 6.86QYY56 pKa = 11.98GLYY59 pKa = 10.45CVGGSVEE66 pKa = 4.73ASFNLAKK73 pKa = 10.57AVVQGLDD80 pKa = 3.6GEE82 pKa = 4.6RR83 pKa = 11.84MGDD86 pKa = 3.46STLSPSIGYY95 pKa = 9.6VGAQVEE101 pKa = 4.88GNYY104 pKa = 7.05EE105 pKa = 4.08TQVTVQSTQGGII117 pKa = 3.37

Molecular weight: 12.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6E3I2|A0A4D6E3I2_9CAUD Uncharacterized protein OS=Microbacterium phage Theresita OX=2562367 GN=28 PE=4 SV=1
MM1 pKa = 7.36NPTEE5 pKa = 4.22RR6 pKa = 11.84LLTKK10 pKa = 10.25IRR12 pKa = 11.84QNPSAARR19 pKa = 11.84RR20 pKa = 11.84VYY22 pKa = 10.53FNKK25 pKa = 9.27TRR27 pKa = 11.84LGTRR31 pKa = 11.84GMNHH35 pKa = 6.3HH36 pKa = 6.33QLATAMIRR44 pKa = 11.84AGISSFSFF52 pKa = 3.41

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12685

35

887

222.5

24.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.72 ± 0.35

0.631 ± 0.144

6.59 ± 0.228

6.212 ± 0.33

2.617 ± 0.183

9.074 ± 0.372

1.585 ± 0.152

4.825 ± 0.431

4.155 ± 0.274

8.262 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.162

3.177 ± 0.204

4.785 ± 0.192

4.005 ± 0.178

6.22 ± 0.392

6.007 ± 0.289

6.724 ± 0.311

7.954 ± 0.3

2.365 ± 0.307

2.838 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski