Circoviridae 19 LDMD-2013
Average proteome isoelectric point is 7.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5TMY4|S5TMY4_9CIRC ATP-dependent helicase Rep OS=Circoviridae 19 LDMD-2013 OX=1379723 PE=3 SV=1
MM1 pKa = 7.23 TSQRR5 pKa = 11.84 SRR7 pKa = 11.84 AWVFTLNNPTSDD19 pKa = 4.14 DD20 pKa = 3.14 ISRR23 pKa = 11.84 LSEE26 pKa = 4.02 CGASYY31 pKa = 10.73 GVYY34 pKa = 10.23 GRR36 pKa = 11.84 EE37 pKa = 3.8 TGEE40 pKa = 4.14 EE41 pKa = 4.63 GTHH44 pKa = 5.99 HH45 pKa = 6.1 LQGYY49 pKa = 9.67 LYY51 pKa = 10.26 FKK53 pKa = 9.84 SQRR56 pKa = 11.84 TLSSLKK62 pKa = 10.19 KK63 pKa = 9.66 KK64 pKa = 10.52 LPRR67 pKa = 11.84 AHH69 pKa = 7.1 LEE71 pKa = 4.01 TRR73 pKa = 11.84 KK74 pKa = 10.32 GSHH77 pKa = 5.07 EE78 pKa = 3.95 QARR81 pKa = 11.84 DD82 pKa = 3.45 YY83 pKa = 10.87 CIKK86 pKa = 10.72 DD87 pKa = 3.38 GNVEE91 pKa = 3.94 EE92 pKa = 5.14 WGTPPCTQKK101 pKa = 11.01 EE102 pKa = 4.11 KK103 pKa = 11.38 GEE105 pKa = 4.51 AGTKK109 pKa = 9.69 VYY111 pKa = 10.79 RR112 pKa = 11.84 EE113 pKa = 3.86 AWNLAKK119 pKa = 10.28 EE120 pKa = 4.14 GRR122 pKa = 11.84 IEE124 pKa = 4.54 EE125 pKa = 3.91 IHH127 pKa = 5.22 EE128 pKa = 4.17 TLRR131 pKa = 11.84 LKK133 pKa = 10.87 YY134 pKa = 10.68 YY135 pKa = 9.49 STLKK139 pKa = 10.48 KK140 pKa = 9.41 IQKK143 pKa = 10.0 DD144 pKa = 3.85 YY145 pKa = 11.0 MVAPPXLDD153 pKa = 3.66 YY154 pKa = 11.5 FEE156 pKa = 5.68 GWWFYY161 pKa = 11.53 GPSGTGKK168 pKa = 9.89 SRR170 pKa = 11.84 KK171 pKa = 9.33 ARR173 pKa = 11.84 LDD175 pKa = 3.3 NPGAYY180 pKa = 9.05 IKK182 pKa = 10.54 NANKK186 pKa = 9.15 WWDD189 pKa = 3.8 GYY191 pKa = 10.74 QGQDD195 pKa = 3.49 VVIIDD200 pKa = 3.82 EE201 pKa = 4.52 WSPSHH206 pKa = 6.99 SVLAHH211 pKa = 6.08 HH212 pKa = 6.83 LKK214 pKa = 10.47 VWVDD218 pKa = 3.46 HH219 pKa = 5.91 YY220 pKa = 11.87 AFNAEE225 pKa = 4.26 VKK227 pKa = 9.79 GGAMMIRR234 pKa = 11.84 PKK236 pKa = 10.63 KK237 pKa = 10.66 VIITSNYY244 pKa = 8.44 TIRR247 pKa = 11.84 EE248 pKa = 4.4 CFTEE252 pKa = 4.14 EE253 pKa = 4.1 QDD255 pKa = 3.92 YY256 pKa = 11.42 LPLTRR261 pKa = 11.84 RR262 pKa = 11.84 FTEE265 pKa = 4.05 VPFGHH270 pKa = 6.82 RR271 pKa = 11.84 GGYY274 pKa = 9.8 VSDD277 pKa = 4.34 FGMEE281 pKa = 4.0 ISQQ284 pKa = 3.97
Molecular weight: 32.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.656
IPC2_protein 7.907
IPC_protein 7.863
Toseland 7.936
ProMoST 8.273
Dawson 8.565
Bjellqvist 8.668
Wikipedia 8.58
Rodwell 8.609
Grimsley 7.922
Solomon 8.712
Lehninger 8.697
Nozaki 8.726
DTASelect 8.521
Thurlkill 8.58
EMBOSS 8.741
Sillero 8.829
Patrickios 4.266
IPC_peptide 8.697
IPC2_peptide 7.527
IPC2.peptide.svr19 7.706
Protein with the highest isoelectric point:
>tr|S5TMY4|S5TMY4_9CIRC ATP-dependent helicase Rep OS=Circoviridae 19 LDMD-2013 OX=1379723 PE=3 SV=1
MM1 pKa = 7.23 TSQRR5 pKa = 11.84 SRR7 pKa = 11.84 AWVFTLNNPTSDD19 pKa = 4.14 DD20 pKa = 3.14 ISRR23 pKa = 11.84 LSEE26 pKa = 4.02 CGASYY31 pKa = 10.73 GVYY34 pKa = 10.23 GRR36 pKa = 11.84 EE37 pKa = 3.8 TGEE40 pKa = 4.14 EE41 pKa = 4.63 GTHH44 pKa = 5.99 HH45 pKa = 6.1 LQGYY49 pKa = 9.67 LYY51 pKa = 10.26 FKK53 pKa = 9.84 SQRR56 pKa = 11.84 TLSSLKK62 pKa = 10.19 KK63 pKa = 9.66 KK64 pKa = 10.52 LPRR67 pKa = 11.84 AHH69 pKa = 7.1 LEE71 pKa = 4.01 TRR73 pKa = 11.84 KK74 pKa = 10.32 GSHH77 pKa = 5.07 EE78 pKa = 3.95 QARR81 pKa = 11.84 DD82 pKa = 3.45 YY83 pKa = 10.87 CIKK86 pKa = 10.72 DD87 pKa = 3.38 GNVEE91 pKa = 3.94 EE92 pKa = 5.14 WGTPPCTQKK101 pKa = 11.01 EE102 pKa = 4.11 KK103 pKa = 11.38 GEE105 pKa = 4.51 AGTKK109 pKa = 9.69 VYY111 pKa = 10.79 RR112 pKa = 11.84 EE113 pKa = 3.86 AWNLAKK119 pKa = 10.28 EE120 pKa = 4.14 GRR122 pKa = 11.84 IEE124 pKa = 4.54 EE125 pKa = 3.91 IHH127 pKa = 5.22 EE128 pKa = 4.17 TLRR131 pKa = 11.84 LKK133 pKa = 10.87 YY134 pKa = 10.68 YY135 pKa = 9.49 STLKK139 pKa = 10.48 KK140 pKa = 9.41 IQKK143 pKa = 10.0 DD144 pKa = 3.85 YY145 pKa = 11.0 MVAPPXLDD153 pKa = 3.66 YY154 pKa = 11.5 FEE156 pKa = 5.68 GWWFYY161 pKa = 11.53 GPSGTGKK168 pKa = 9.89 SRR170 pKa = 11.84 KK171 pKa = 9.33 ARR173 pKa = 11.84 LDD175 pKa = 3.3 NPGAYY180 pKa = 9.05 IKK182 pKa = 10.54 NANKK186 pKa = 9.15 WWDD189 pKa = 3.8 GYY191 pKa = 10.74 QGQDD195 pKa = 3.49 VVIIDD200 pKa = 3.82 EE201 pKa = 4.52 WSPSHH206 pKa = 6.99 SVLAHH211 pKa = 6.08 HH212 pKa = 6.83 LKK214 pKa = 10.47 VWVDD218 pKa = 3.46 HH219 pKa = 5.91 YY220 pKa = 11.87 AFNAEE225 pKa = 4.26 VKK227 pKa = 9.79 GGAMMIRR234 pKa = 11.84 PKK236 pKa = 10.63 KK237 pKa = 10.66 VIITSNYY244 pKa = 8.44 TIRR247 pKa = 11.84 EE248 pKa = 4.4 CFTEE252 pKa = 4.14 EE253 pKa = 4.1 QDD255 pKa = 3.92 YY256 pKa = 11.42 LPLTRR261 pKa = 11.84 RR262 pKa = 11.84 FTEE265 pKa = 4.05 VPFGHH270 pKa = 6.82 RR271 pKa = 11.84 GGYY274 pKa = 9.8 VSDD277 pKa = 4.34 FGMEE281 pKa = 4.0 ISQQ284 pKa = 3.97
Molecular weight: 32.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.656
IPC2_protein 7.907
IPC_protein 7.863
Toseland 7.936
ProMoST 8.273
Dawson 8.565
Bjellqvist 8.668
Wikipedia 8.58
Rodwell 8.609
Grimsley 7.922
Solomon 8.712
Lehninger 8.697
Nozaki 8.726
DTASelect 8.521
Thurlkill 8.58
EMBOSS 8.741
Sillero 8.829
Patrickios 4.266
IPC_peptide 8.697
IPC2_peptide 7.527
IPC2.peptide.svr19 7.706
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
284
284
284
284.0
32.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.282 ± 0.0
1.408 ± 0.0
4.577 ± 0.0
8.099 ± 0.0
3.169 ± 0.0
8.803 ± 0.0
3.521 ± 0.0
4.577 ± 0.0
7.746 ± 0.0
6.338 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.761 ± 0.0
3.169 ± 0.0
4.225 ± 0.0
3.521 ± 0.0
6.338 ± 0.0
6.69 ± 0.0
6.338 ± 0.0
4.93 ± 0.0
3.169 ± 0.0
5.986 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here