Hart Park virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Hapavirus; Hart Park hapavirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T2FGV0|T2FGV0_9RHAB U1 protein OS=Hart Park virus OX=200401 GN=U1 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 5.85LYY4 pKa = 10.46IHH6 pKa = 6.96LGITFHH12 pKa = 6.94YY13 pKa = 9.77NGSYY17 pKa = 10.71LDD19 pKa = 3.87NDD21 pKa = 3.55NLNWIISRR29 pKa = 11.84MIYY32 pKa = 10.41DD33 pKa = 4.43CVRR36 pKa = 11.84EE37 pKa = 4.18SGVPGDD43 pKa = 3.8VAAFAINLAWEE54 pKa = 4.29HH55 pKa = 6.37VDD57 pKa = 3.05ICFDD61 pKa = 3.74SGNITHH67 pKa = 7.17GYY69 pKa = 9.48CWLQEE74 pKa = 4.02AVSLPGTPKK83 pKa = 10.94DD84 pKa = 3.96LDD86 pKa = 3.63KK87 pKa = 11.71LNNTFSSRR95 pKa = 11.84GSFFIQGDD103 pKa = 3.62EE104 pKa = 4.05DD105 pKa = 4.04LGEE108 pKa = 3.93VEE110 pKa = 5.16YY111 pKa = 10.62IFFVAEE117 pKa = 3.69PTLQEE122 pKa = 3.76GHH124 pKa = 6.69PWDD127 pKa = 4.6VVWHH131 pKa = 5.97PMFVDD136 pKa = 4.15PQAYY140 pKa = 9.38HH141 pKa = 7.0IKK143 pKa = 10.42RR144 pKa = 11.84NPDD147 pKa = 2.63VVAYY151 pKa = 9.49KK152 pKa = 10.5FRR154 pKa = 11.84FQHH157 pKa = 6.88LIHH160 pKa = 6.95PP161 pKa = 5.17

Molecular weight: 18.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R1J5|A0A0D3R1J5_9RHAB Glycoprotein OS=Hart Park virus OX=200401 PE=4 SV=1
MM1 pKa = 7.38GFDD4 pKa = 3.13IGGDD8 pKa = 2.8IGKK11 pKa = 9.03PLKK14 pKa = 10.65DD15 pKa = 3.45AFDD18 pKa = 3.87KK19 pKa = 11.25FGADD23 pKa = 2.6IKK25 pKa = 9.46MTFLTVLNWMKK36 pKa = 10.06WISIGILIVISVILICKK53 pKa = 9.05IIKK56 pKa = 10.01VLFQCGKK63 pKa = 10.27CLLSCFGFCKK73 pKa = 10.35KK74 pKa = 9.83CVKK77 pKa = 10.35GNHH80 pKa = 4.7SHH82 pKa = 5.82MNKK85 pKa = 8.12TRR87 pKa = 11.84KK88 pKa = 9.03KK89 pKa = 7.91HH90 pKa = 4.24QFRR93 pKa = 11.84GKK95 pKa = 8.19VKK97 pKa = 10.4KK98 pKa = 8.1MTVPVIRR105 pKa = 11.84KK106 pKa = 8.55KK107 pKa = 10.94VKK109 pKa = 9.22IRR111 pKa = 11.84KK112 pKa = 9.21DD113 pKa = 3.27PSLVEE118 pKa = 4.02LVV120 pKa = 3.45

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4224

120

2104

469.3

53.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.859 ± 0.514

1.847 ± 0.228

5.611 ± 0.644

5.682 ± 0.387

4.498 ± 0.263

5.611 ± 0.522

2.438 ± 0.232

7.884 ± 0.312

6.818 ± 0.551

10.038 ± 0.974

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.344

5.8 ± 0.522

4.451 ± 0.484

3.717 ± 0.302

4.64 ± 0.248

8.002 ± 0.55

4.853 ± 0.39

5.469 ± 0.42

2.107 ± 0.233

4.214 ± 0.494
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski