Vibrio phage JSF7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Tawavirus; Vibrio virus JSF7

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A240EWV2|A0A240EWV2_9CAUD Uncharacterized protein OS=Vibrio phage JSF7 OX=1292086 PE=4 SV=1
MM1 pKa = 8.41DD2 pKa = 4.52INLWRR7 pKa = 11.84KK8 pKa = 9.89SYY10 pKa = 9.64TVDD13 pKa = 3.64ARR15 pKa = 11.84ALFSNASLVAMKK27 pKa = 10.47ADD29 pKa = 3.54YY30 pKa = 10.48LQLIEE35 pKa = 4.6EE36 pKa = 4.22QPEE39 pKa = 4.03LLMNSAEE46 pKa = 4.2TLYY49 pKa = 11.6DD50 pKa = 3.49MLCQGNQVIEE60 pKa = 4.31VTQVLMYY67 pKa = 9.16FSNQAQCDD75 pKa = 3.62TAMDD79 pKa = 4.15EE80 pKa = 4.66YY81 pKa = 10.13YY82 pKa = 10.42TIIRR86 pKa = 11.84GEE88 pKa = 4.06VV89 pKa = 2.85

Molecular weight: 10.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A219XCZ9|A0A219XCZ9_9CAUD Uncharacterized protein OS=Vibrio phage JSF7 OX=1292086 PE=4 SV=1
MM1 pKa = 7.42LKK3 pKa = 10.54ARR5 pKa = 11.84GIDD8 pKa = 3.22KK9 pKa = 10.81RR10 pKa = 11.84EE11 pKa = 3.85VVVRR15 pKa = 11.84LHH17 pKa = 6.05TNTWLHH23 pKa = 5.35TVQPDD28 pKa = 3.26AAASLLTRR36 pKa = 11.84TVRR39 pKa = 11.84DD40 pKa = 3.24QVHH43 pKa = 4.37QVARR47 pKa = 11.84LAGVSIYY54 pKa = 9.18WDD56 pKa = 3.61RR57 pKa = 11.84YY58 pKa = 9.21DD59 pKa = 5.22GIVGTRR65 pKa = 11.84GLEE68 pKa = 3.69VNMRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 10.25VYY76 pKa = 9.58TGPPHH81 pKa = 7.4AGRR84 pKa = 11.84SKK86 pKa = 11.02ANTVKK91 pKa = 9.91HH92 pKa = 5.89YY93 pKa = 10.11YY94 pKa = 8.72MIRR97 pKa = 11.84RR98 pKa = 11.84VYY100 pKa = 10.23WEE102 pKa = 4.04VLSAAVINGRR112 pKa = 11.84CTKK115 pKa = 10.39KK116 pKa = 10.99LEE118 pKa = 5.2DD119 pKa = 3.83ISNGNASSKK128 pKa = 10.99

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

14120

61

1683

288.2

32.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.101 ± 0.599

1.126 ± 0.161

5.673 ± 0.251

6.402 ± 0.259

3.499 ± 0.175

6.997 ± 0.318

2.082 ± 0.181

4.738 ± 0.277

5.949 ± 0.325

7.797 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.399 ± 0.178

5.028 ± 0.368

4.285 ± 0.269

4.618 ± 0.343

5.453 ± 0.244

5.326 ± 0.194

5.574 ± 0.295

7.443 ± 0.267

1.806 ± 0.129

3.704 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski