Thermomonas sp. SY21
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2753 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B7ZTF1|A0A5B7ZTF1_9GAMM Uncharacterized protein OS=Thermomonas sp. SY21 OX=2202149 GN=FHQ07_06950 PE=4 SV=1
MM1 pKa = 7.65 SDD3 pKa = 3.12 SAAVDD8 pKa = 3.36 APYY11 pKa = 8.83 RR12 pKa = 11.84 TWMCVVCGFVYY23 pKa = 10.68 DD24 pKa = 4.02 EE25 pKa = 4.77 ALGLPEE31 pKa = 6.22 DD32 pKa = 4.81 GIEE35 pKa = 4.57 PGTRR39 pKa = 11.84 WEE41 pKa = 5.53 DD42 pKa = 3.72 IPDD45 pKa = 3.14 TWTCPDD51 pKa = 4.14 CGVSKK56 pKa = 11.13 DD57 pKa = 3.95 DD58 pKa = 3.8 FEE60 pKa = 5.87 LMRR63 pKa = 11.84 GG64 pKa = 3.51
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.808
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.783
Patrickios 0.693
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.728
Protein with the highest isoelectric point:
>tr|A0A5B7ZNZ4|A0A5B7ZNZ4_9GAMM NADH-quinone oxidoreductase OS=Thermomonas sp. SY21 OX=2202149 GN=nuoG PE=3 SV=1
MM1 pKa = 7.15 STGTCAMRR9 pKa = 11.84 LPGRR13 pKa = 11.84 STNSAISTTPRR24 pKa = 11.84 TCAPPISRR32 pKa = 11.84 DD33 pKa = 2.98 WKK35 pKa = 9.8 RR36 pKa = 11.84 PPGKK40 pKa = 9.36 RR41 pKa = 11.84 ARR43 pKa = 11.84 RR44 pKa = 11.84 AWRR47 pKa = 11.84 MPACCRR53 pKa = 11.84 PRR55 pKa = 11.84 WRR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 GRR61 pKa = 11.84 DD62 pKa = 2.85 RR63 pKa = 11.84 AAFPLGGRR71 pKa = 11.84 PGSRR75 pKa = 11.84 RR76 pKa = 11.84 LAASWPPRR84 pKa = 11.84 VRR86 pKa = 11.84 LGVGLGYY93 pKa = 7.78 GTCAGPMEE101 pKa = 4.75 SAMHH105 pKa = 6.27 APNRR109 pKa = 11.84 FRR111 pKa = 11.84 IALLLCGGALAAGGAMAARR130 pKa = 11.84 PAASAGEE137 pKa = 4.34 CFASAFEE144 pKa = 4.88 SGDD147 pKa = 3.15 ADD149 pKa = 5.44 AIAACYY155 pKa = 10.6 AEE157 pKa = 5.63 DD158 pKa = 4.97 AIIWFPGGSMAKK170 pKa = 9.78 GRR172 pKa = 11.84 AAIRR176 pKa = 11.84 DD177 pKa = 3.91 GFAHH181 pKa = 6.73 FLAGATVKK189 pKa = 10.44 DD190 pKa = 4.0 FQMTNIGQEE199 pKa = 4.21 SVGDD203 pKa = 3.92 SRR205 pKa = 11.84 VSWGTYY211 pKa = 7.27 VIRR214 pKa = 11.84 MVDD217 pKa = 3.11 KK218 pKa = 10.73 ASQAEE223 pKa = 4.36 SVQTGRR229 pKa = 11.84 YY230 pKa = 7.43 TDD232 pKa = 3.45 VQKK235 pKa = 11.13 LVDD238 pKa = 4.23 GRR240 pKa = 11.84 WLYY243 pKa = 10.56 IVDD246 pKa = 4.71 HH247 pKa = 7.09 PSDD250 pKa = 4.73 DD251 pKa = 3.87 PAPPAKK257 pKa = 10.44
Molecular weight: 27.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.414
IPC_protein 10.189
Toseland 10.35
ProMoST 10.175
Dawson 10.511
Bjellqvist 10.277
Wikipedia 10.73
Rodwell 10.599
Grimsley 10.57
Solomon 10.613
Lehninger 10.57
Nozaki 10.438
DTASelect 10.248
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.452
Patrickios 10.277
IPC_peptide 10.613
IPC2_peptide 9.663
IPC2.peptide.svr19 8.461
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2753
0
2753
912151
32
2007
331.3
35.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.764 ± 0.083
0.86 ± 0.016
6.076 ± 0.036
5.268 ± 0.042
3.412 ± 0.03
8.761 ± 0.053
2.188 ± 0.025
4.215 ± 0.03
3.173 ± 0.04
10.745 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.261 ± 0.02
2.529 ± 0.034
5.211 ± 0.038
3.625 ± 0.028
7.698 ± 0.054
4.894 ± 0.037
4.422 ± 0.042
7.041 ± 0.038
1.533 ± 0.021
2.324 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here