Yersinia phage YeP4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6B541|A0A5J6B541_9CAUD Uncharacterized protein OS=Yersinia phage YeP4 OX=2562812 PE=4 SV=1
MM1 pKa = 7.63ARR3 pKa = 11.84PKK5 pKa = 10.35TKK7 pKa = 9.9IEE9 pKa = 4.11VPGQEE14 pKa = 4.23TLQDD18 pKa = 3.92DD19 pKa = 4.15ATDD22 pKa = 5.13LIGTAGEE29 pKa = 4.3DD30 pKa = 2.85TGTALTVAEE39 pKa = 4.96ALTGLDD45 pKa = 3.45SNATTEE51 pKa = 4.07EE52 pKa = 4.45LPAVDD57 pKa = 3.15HH58 pKa = 5.7TARR61 pKa = 11.84NEE63 pKa = 3.76ALTGINAAGFTLITRR78 pKa = 11.84FEE80 pKa = 4.04EE81 pKa = 4.27LGFVDD86 pKa = 6.37AIGHH90 pKa = 6.7PLTNCLDD97 pKa = 4.12FIEE100 pKa = 4.77LVKK103 pKa = 10.74QATTEE108 pKa = 4.07KK109 pKa = 8.89QTGAGSVGSVGAIGGASQTLTDD131 pKa = 4.72AGWIVQQ137 pKa = 3.62

Molecular weight: 14.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6B684|A0A5J6B684_9CAUD Uncharacterized protein OS=Yersinia phage YeP4 OX=2562812 PE=4 SV=1
MM1 pKa = 7.75KK2 pKa = 9.58LTDD5 pKa = 3.5TKK7 pKa = 10.75IRR9 pKa = 11.84NAKK12 pKa = 8.31PQEE15 pKa = 4.01KK16 pKa = 9.88AYY18 pKa = 10.48QLQDD22 pKa = 3.33GNGLYY27 pKa = 10.64LDD29 pKa = 3.98VRR31 pKa = 11.84PSGVKK36 pKa = 6.49TWRR39 pKa = 11.84YY40 pKa = 9.37RR41 pKa = 11.84YY42 pKa = 8.76WVTPTKK48 pKa = 10.43DD49 pKa = 2.95GRR51 pKa = 11.84YY52 pKa = 8.23TIGSYY57 pKa = 10.04PAVSLAEE64 pKa = 3.89ARR66 pKa = 11.84KK67 pKa = 8.02VRR69 pKa = 11.84EE70 pKa = 3.88WARR73 pKa = 11.84EE74 pKa = 3.83QVKK77 pKa = 10.76SGIVPKK83 pKa = 9.87EE84 pKa = 3.75AKK86 pKa = 9.1ATEE89 pKa = 3.87RR90 pKa = 11.84DD91 pKa = 3.22IAKK94 pKa = 10.39AEE96 pKa = 4.07SANTFEE102 pKa = 4.22IVARR106 pKa = 11.84EE107 pKa = 3.9WMAKK111 pKa = 9.64KK112 pKa = 9.34WDD114 pKa = 3.53NWSVNTKK121 pKa = 8.83NQIIAFMDD129 pKa = 3.47NDD131 pKa = 3.7IFPAIGGMPMRR142 pKa = 11.84EE143 pKa = 3.8INASHH148 pKa = 6.77ILKK151 pKa = 10.29IIRR154 pKa = 11.84EE155 pKa = 4.29LEE157 pKa = 3.7NRR159 pKa = 11.84GAISVAVKK167 pKa = 9.32TRR169 pKa = 11.84QWCSAIFCYY178 pKa = 10.27GVATLRR184 pKa = 11.84ADD186 pKa = 3.74SDD188 pKa = 3.62PAAALKK194 pKa = 10.58GAIITPKK201 pKa = 9.66IVHH204 pKa = 6.0SRR206 pKa = 11.84SLTGDD211 pKa = 3.5EE212 pKa = 4.51LRR214 pKa = 11.84DD215 pKa = 3.41YY216 pKa = 10.95YY217 pKa = 11.4LRR219 pKa = 11.84LEE221 pKa = 4.66SYY223 pKa = 8.97TGTRR227 pKa = 11.84ATIIALKK234 pKa = 10.03ILPLVFVRR242 pKa = 11.84QVEE245 pKa = 4.16LRR247 pKa = 11.84SAEE250 pKa = 3.95WAHH253 pKa = 7.18IDD255 pKa = 3.63FDD257 pKa = 3.85NAEE260 pKa = 4.03WVIPAEE266 pKa = 3.97LMKK269 pKa = 9.87MRR271 pKa = 11.84RR272 pKa = 11.84IHH274 pKa = 6.81RR275 pKa = 11.84VPLSKK280 pKa = 10.36PVLSLLHH287 pKa = 6.03EE288 pKa = 4.66LKK290 pKa = 10.73EE291 pKa = 4.33LTGKK295 pKa = 10.13RR296 pKa = 11.84RR297 pKa = 11.84WLFPNTRR304 pKa = 11.84LDD306 pKa = 3.64TFMGASTLNWAIVHH320 pKa = 5.92MGYY323 pKa = 10.23GRR325 pKa = 11.84KK326 pKa = 9.35VLTTHH331 pKa = 7.3DD332 pKa = 3.92FRR334 pKa = 11.84ATASTRR340 pKa = 11.84LHH342 pKa = 5.52EE343 pKa = 4.05MGYY346 pKa = 9.02RR347 pKa = 11.84HH348 pKa = 6.95DD349 pKa = 4.07VIEE352 pKa = 4.3RR353 pKa = 11.84QLAHH357 pKa = 5.58VEE359 pKa = 4.15KK360 pKa = 10.56NRR362 pKa = 11.84VSAAYY367 pKa = 10.08NHH369 pKa = 6.74AEE371 pKa = 3.88YY372 pKa = 9.83MPEE375 pKa = 3.8RR376 pKa = 11.84RR377 pKa = 11.84EE378 pKa = 4.39LMEE381 pKa = 4.22GWGRR385 pKa = 11.84WVTGLLAADD394 pKa = 4.25SPQQ397 pKa = 2.9

Molecular weight: 45.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

10718

39

841

274.8

30.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.056 ± 0.553

0.914 ± 0.148

6.102 ± 0.221

6.26 ± 0.337

3.387 ± 0.139

7.436 ± 0.314

1.782 ± 0.165

5.43 ± 0.183

5.542 ± 0.338

8.444 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.145

4.544 ± 0.252

4.003 ± 0.206

4.46 ± 0.303

5.542 ± 0.277

5.701 ± 0.291

6.307 ± 0.282

6.54 ± 0.362

1.577 ± 0.121

2.724 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski