Acidianus two-tailed virus (ATV)
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q3V4T4|Y059A_ATV Uncharacterized protein ORF59a OS=Acidianus two-tailed virus OX=315953 PE=4 SV=1
MM1 pKa = 7.39 NCLEE5 pKa = 4.09 ILISKK10 pKa = 8.82 GYY12 pKa = 10.49 NMQGYY17 pKa = 9.6 SPDD20 pKa = 3.6 TLLLLSEE27 pKa = 4.58 KK28 pKa = 10.28 EE29 pKa = 4.21 GNDD32 pKa = 3.4 VEE34 pKa = 4.63 EE35 pKa = 4.9 CEE37 pKa = 6.09 AYY39 pKa = 10.46 FSWLEE44 pKa = 3.8 AVYY47 pKa = 10.64 GG48 pKa = 3.85
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.966
IPC2_protein 3.999
IPC_protein 3.757
Toseland 3.617
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.617
Grimsley 3.554
Solomon 3.668
Lehninger 3.617
Nozaki 3.872
DTASelect 3.897
Thurlkill 3.681
EMBOSS 3.63
Sillero 3.872
Patrickios 0.006
IPC_peptide 3.668
IPC2_peptide 3.846
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>sp|Q3V4V9|Y059B_ATV Uncharacterized protein ORF59b OS=Acidianus two-tailed virus OX=315953 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.35 VLSFLIRR9 pKa = 11.84 QDD11 pKa = 3.13 HH12 pKa = 6.7 LLYY15 pKa = 10.82 LDD17 pKa = 4.81 IFAKK21 pKa = 10.39 QNNLTRR27 pKa = 11.84 SDD29 pKa = 4.17 AIRR32 pKa = 11.84 YY33 pKa = 7.73 AISVLDD39 pKa = 4.01 EE40 pKa = 4.2 EE41 pKa = 4.77 STPVSLVGFPGIKK54 pKa = 9.77 LVRR57 pKa = 11.84 TSVKK61 pKa = 10.06 LAEE64 pKa = 4.24 NVISRR69 pKa = 11.84 IDD71 pKa = 3.28 RR72 pKa = 11.84 LAILSKK78 pKa = 9.49 ITRR81 pKa = 11.84 SDD83 pKa = 3.56 VIRR86 pKa = 11.84 NAIYY90 pKa = 10.45 HH91 pKa = 6.35 LLINNAPKK99 pKa = 10.16 QLPPVTAQTEE109 pKa = 4.14 KK110 pKa = 10.64 KK111 pKa = 9.56 YY112 pKa = 10.98 GYY114 pKa = 9.03 VCPYY118 pKa = 9.15 CVSRR122 pKa = 11.84 FPTVRR127 pKa = 11.84 ALKK130 pKa = 8.94 IHH132 pKa = 6.98 LKK134 pKa = 9.86 RR135 pKa = 11.84 RR136 pKa = 11.84 HH137 pKa = 6.39 NGFPWCPVCYY147 pKa = 10.14 KK148 pKa = 10.29 PLKK151 pKa = 10.0 NKK153 pKa = 9.98 NATNHH158 pKa = 5.26 FRR160 pKa = 11.84 RR161 pKa = 11.84 FTDD164 pKa = 3.62 PQHH167 pKa = 5.95 QFWYY171 pKa = 8.34 MISRR175 pKa = 11.84 KK176 pKa = 9.46 RR177 pKa = 11.84 YY178 pKa = 8.01 LSSHH182 pKa = 6.05 RR183 pKa = 11.84 KK184 pKa = 8.47 EE185 pKa = 4.12 AVKK188 pKa = 10.47 QQ189 pKa = 3.48
Molecular weight: 22.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.706
IPC_protein 10.058
Toseland 10.423
ProMoST 10.058
Dawson 10.57
Bjellqvist 10.248
Wikipedia 10.745
Rodwell 11.008
Grimsley 10.628
Solomon 10.613
Lehninger 10.584
Nozaki 10.423
DTASelect 10.233
Thurlkill 10.438
EMBOSS 10.818
Sillero 10.496
Patrickios 10.687
IPC_peptide 10.613
IPC2_peptide 9.121
IPC2.peptide.svr19 8.619
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
19264
34
1940
267.6
30.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.975 ± 0.317
0.54 ± 0.087
4.532 ± 0.423
7.283 ± 0.751
4.36 ± 0.317
5.248 ± 0.412
1.355 ± 0.244
7.413 ± 0.291
7.807 ± 0.653
9.619 ± 0.437
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.139 ± 0.17
5.347 ± 0.309
5.253 ± 0.546
4.033 ± 0.328
3.774 ± 0.446
6.868 ± 0.556
6.099 ± 0.575
6.759 ± 0.38
0.877 ± 0.127
4.719 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here