Tannerella sp. CAG:51
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2586 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7DEX2|R7DEX2_9BACT Uncharacterized protein OS=Tannerella sp. CAG:51 OX=1262979 GN=BN686_01645 PE=4 SV=1
MM1 pKa = 7.26 SQQDD5 pKa = 3.75 DD6 pKa = 3.98 LQYY9 pKa = 11.56 DD10 pKa = 3.3 EE11 pKa = 6.03 DD12 pKa = 4.58 DD13 pKa = 3.28 AVKK16 pKa = 10.28 FIRR19 pKa = 11.84 NFLPGEE25 pKa = 4.2 VASKK29 pKa = 10.75 FSDD32 pKa = 3.57 DD33 pKa = 4.69 DD34 pKa = 3.68 INYY37 pKa = 9.97 VIDD40 pKa = 5.34 LIYY43 pKa = 10.73 EE44 pKa = 4.55 FYY46 pKa = 10.6 EE47 pKa = 4.12 SKK49 pKa = 11.21 GYY51 pKa = 9.33 MDD53 pKa = 4.95 EE54 pKa = 5.05 NNDD57 pKa = 3.66 SEE59 pKa = 4.4 VDD61 pKa = 3.55 IIEE64 pKa = 5.09 DD65 pKa = 3.61 EE66 pKa = 4.04 LTEE69 pKa = 4.02 YY70 pKa = 11.13 VITNARR76 pKa = 11.84 KK77 pKa = 9.58 NGFTRR82 pKa = 11.84 FDD84 pKa = 3.91 ADD86 pKa = 3.72 DD87 pKa = 3.53 VSMVVQGEE95 pKa = 4.36 LEE97 pKa = 4.22 YY98 pKa = 10.95 CDD100 pKa = 3.98 SIGMFEE106 pKa = 3.94
Molecular weight: 12.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 3.579
IPC_protein 3.567
Toseland 3.35
ProMoST 3.732
Dawson 3.567
Bjellqvist 3.77
Wikipedia 3.516
Rodwell 3.401
Grimsley 3.261
Solomon 3.554
Lehninger 3.503
Nozaki 3.694
DTASelect 3.91
Thurlkill 3.414
EMBOSS 3.528
Sillero 3.694
Patrickios 1.812
IPC_peptide 3.541
IPC2_peptide 3.668
IPC2.peptide.svr19 3.702
Protein with the highest isoelectric point:
>tr|R7DIN2|R7DIN2_9BACT 30S ribosomal protein S18 OS=Tannerella sp. CAG:51 OX=1262979 GN=rpsR PE=3 SV=1
MM1 pKa = 7.55 KK2 pKa = 10.21 RR3 pKa = 11.84 RR4 pKa = 11.84 TVLSIVLLSLVGWCGAYY21 pKa = 10.28 AQVQVADD28 pKa = 3.82 TSIVQSLEE36 pKa = 3.82 RR37 pKa = 11.84 VTGNARR43 pKa = 11.84 VRR45 pKa = 11.84 LFQDD49 pKa = 2.62 ARR51 pKa = 11.84 LTDD54 pKa = 3.59 RR55 pKa = 11.84 LARR58 pKa = 11.84 RR59 pKa = 11.84 TGNVALSGTSSRR71 pKa = 11.84 DD72 pKa = 3.25 MNYY75 pKa = 7.95 VTVRR79 pKa = 11.84 GYY81 pKa = 10.64 RR82 pKa = 11.84 IQVFSDD88 pKa = 2.83 NNQRR92 pKa = 11.84 RR93 pKa = 11.84 SKK95 pKa = 11.29 DD96 pKa = 3.15 EE97 pKa = 4.5 AYY99 pKa = 10.62 QKK101 pKa = 11.61 ASMIKK106 pKa = 10.45 DD107 pKa = 3.44 ADD109 pKa = 4.24 PEE111 pKa = 4.19 LSTYY115 pKa = 8.28 VTFTSPFWRR124 pKa = 11.84 LRR126 pKa = 11.84 VGDD129 pKa = 3.71 FRR131 pKa = 11.84 SFEE134 pKa = 4.01 EE135 pKa = 4.46 ANLKK139 pKa = 10.54 LIEE142 pKa = 4.67 LKK144 pKa = 9.47 NTFPQFRR151 pKa = 11.84 EE152 pKa = 3.78 MRR154 pKa = 11.84 VVKK157 pKa = 10.61 DD158 pKa = 3.56 MIRR161 pKa = 11.84 LTRR164 pKa = 11.84 IVEE167 pKa = 4.01
Molecular weight: 19.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.516
IPC_protein 10.233
Toseland 10.379
ProMoST 10.101
Dawson 10.54
Bjellqvist 10.248
Wikipedia 10.745
Rodwell 10.716
Grimsley 10.599
Solomon 10.613
Lehninger 10.584
Nozaki 10.365
DTASelect 10.248
Thurlkill 10.409
EMBOSS 10.789
Sillero 10.452
Patrickios 10.409
IPC_peptide 10.613
IPC2_peptide 9.107
IPC2.peptide.svr19 8.687
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2586
0
2586
968938
29
2488
374.7
42.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.428 ± 0.047
1.187 ± 0.017
5.606 ± 0.037
6.348 ± 0.044
4.787 ± 0.034
6.766 ± 0.044
1.739 ± 0.019
7.923 ± 0.053
6.935 ± 0.038
8.926 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.533 ± 0.021
5.393 ± 0.044
3.822 ± 0.023
3.253 ± 0.027
4.415 ± 0.038
6.575 ± 0.033
5.456 ± 0.04
6.176 ± 0.046
1.225 ± 0.019
4.503 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here