Phenylobacterium sp. Root1277

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Phenylobacterium; unclassified Phenylobacterium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4022 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7D775|A0A0Q7D775_9CAUL Uncharacterized protein OS=Phenylobacterium sp. Root1277 OX=1736442 GN=ASC73_17545 PE=4 SV=1
MM1 pKa = 7.17FVDD4 pKa = 3.81TRR6 pKa = 11.84VEE8 pKa = 4.25GYY10 pKa = 7.24EE11 pKa = 4.1TIVTGVAPGAEE22 pKa = 4.19VVLIDD27 pKa = 4.56ASGDD31 pKa = 3.3GVAQMAQALAGRR43 pKa = 11.84TDD45 pKa = 2.72ITAIHH50 pKa = 6.37IVGHH54 pKa = 5.26GQAGVMMLGSATLGAGDD71 pKa = 3.33IDD73 pKa = 4.4AYY75 pKa = 10.65AGEE78 pKa = 4.15LRR80 pKa = 11.84AIGGALAPDD89 pKa = 4.49GDD91 pKa = 3.92IQLWGCEE98 pKa = 3.73IGAGPEE104 pKa = 3.64GAAFIQALAAATGADD119 pKa = 3.2IAASDD124 pKa = 4.53DD125 pKa = 3.6LTGAGGDD132 pKa = 3.49WVLEE136 pKa = 4.37TQTGTIEE143 pKa = 4.04TSVAITPAGQHH154 pKa = 5.82AFAGTLAIGSEE165 pKa = 4.26NFDD168 pKa = 3.61SLGLITTFGTTLTAGNWQFTSGSAADD194 pKa = 3.88HH195 pKa = 6.36AVASSPEE202 pKa = 3.86YY203 pKa = 10.76AVWLNNDD210 pKa = 3.09GGASDD215 pKa = 3.88RR216 pKa = 11.84AVILNYY222 pKa = 10.8NGDD225 pKa = 4.03NITNYY230 pKa = 10.76AMKK233 pKa = 10.61SSDD236 pKa = 3.18GTNFALQSFKK246 pKa = 10.53IGQSAGATQTLTIAAYY262 pKa = 9.8SNGVQVVAPEE272 pKa = 4.32TVNLAASDD280 pKa = 4.1SIGGVNYY287 pKa = 10.79ALGGNDD293 pKa = 3.15GSGAYY298 pKa = 7.58GTLTFSSAYY307 pKa = 10.66ANIDD311 pKa = 3.32EE312 pKa = 4.3IRR314 pKa = 11.84FVFGGLSDD322 pKa = 4.31LHH324 pKa = 8.4IDD326 pKa = 4.87DD327 pKa = 4.48IAVAPAVVDD336 pKa = 3.38VTAPVFVSAAVNGATLVMTYY356 pKa = 10.78NEE358 pKa = 5.7AITLDD363 pKa = 3.81AVNAPAAGRR372 pKa = 11.84FAVMADD378 pKa = 3.57GASVTVNSVTVNAAAKK394 pKa = 8.86TVTLTLQSAVTAGQAVTVAYY414 pKa = 8.05TDD416 pKa = 3.52PTGGDD421 pKa = 3.59DD422 pKa = 4.74ASAIQDD428 pKa = 3.51AVGNDD433 pKa = 3.72AASLPATSVTNNTVDD448 pKa = 3.74PPPLVSGNIAVPADD462 pKa = 3.35GSYY465 pKa = 11.08AAGQTLSFTVTFTEE479 pKa = 4.06NVTVVGTDD487 pKa = 3.09SVLGLTIGSTARR499 pKa = 11.84EE500 pKa = 3.92AVFASSTANSVTYY513 pKa = 10.14AYY515 pKa = 8.44TVQAGDD521 pKa = 3.27TDD523 pKa = 4.49ANGVAVGALNLNTSTIRR540 pKa = 11.84DD541 pKa = 3.69VAGNDD546 pKa = 3.13ANLALGGHH554 pKa = 5.78VPSTAGVLVDD564 pKa = 3.37TTVPEE569 pKa = 4.24VATVSVPSGGTYY581 pKa = 10.63AAGQTLSFTVNFDD594 pKa = 4.21DD595 pKa = 3.98NVTVAGTDD603 pKa = 3.2STLALTIGATARR615 pKa = 11.84TATYY619 pKa = 10.49LSKK622 pKa = 9.49TANSITYY629 pKa = 8.48TYY631 pKa = 9.39TVQAGEE637 pKa = 4.11TDD639 pKa = 3.15ADD641 pKa = 4.81GITVGALSKK650 pKa = 10.8GSSTIRR656 pKa = 11.84DD657 pKa = 3.4AAGNDD662 pKa = 3.27AVLTLNSVGATSGVLVDD679 pKa = 3.55ATVPTVSGNISVPTGGMYY697 pKa = 9.46MAGQTLSFTVTFDD710 pKa = 3.58EE711 pKa = 4.31NVTLSGSPSTLGLTIGAVSRR731 pKa = 11.84SADD734 pKa = 3.18ITGTTGNSITYY745 pKa = 9.37SYY747 pKa = 9.82TVQASDD753 pKa = 3.53YY754 pKa = 9.32DD755 pKa = 3.95ADD757 pKa = 4.83GIAVTGISLNGGTIRR772 pKa = 11.84DD773 pKa = 3.86GAGNNADD780 pKa = 3.82LTLSGHH786 pKa = 6.48LPSLAGVLVDD796 pKa = 4.37GGPPAVSGNIAVPANGSYY814 pKa = 11.39GEE816 pKa = 4.4GATLTFTVTFGEE828 pKa = 4.41NVTVTGSDD836 pKa = 3.13SVLGLTIGSIARR848 pKa = 11.84EE849 pKa = 3.91ATYY852 pKa = 10.71ASSSGASVTYY862 pKa = 10.14AYY864 pKa = 8.54TVQPGEE870 pKa = 3.91TDD872 pKa = 3.12ANGIAIGAVNLNTSTIKK889 pKa = 10.78DD890 pKa = 3.33AAGNHH895 pKa = 6.24ANLALGGHH903 pKa = 6.08VPATSGILVDD913 pKa = 3.89TTAPGVASVSVPSSATYY930 pKa = 10.57VAGQNLDD937 pKa = 4.01FIVNFDD943 pKa = 3.82EE944 pKa = 4.56AVTIAGSDD952 pKa = 3.56SVIALMVGSTSRR964 pKa = 11.84SATFFSSSGSTATYY978 pKa = 10.0RR979 pKa = 11.84YY980 pKa = 7.06TVQAGDD986 pKa = 3.32VDD988 pKa = 4.08ADD990 pKa = 4.48GITVGALSKK999 pKa = 10.8GSSTIRR1005 pKa = 11.84DD1006 pKa = 3.54SAGNDD1011 pKa = 3.15AEE1013 pKa = 4.39LTLNSVGATNGVMIDD1028 pKa = 3.51GTVPSVTGNVSVPANDD1044 pKa = 3.64TYY1046 pKa = 11.81VVGEE1050 pKa = 4.44TPSFTVTFDD1059 pKa = 3.42EE1060 pKa = 4.41NVTVTGTGSTLGLTIGATARR1080 pKa = 11.84TAAYY1084 pKa = 9.98ASKK1087 pKa = 8.46TANSITYY1094 pKa = 8.48TYY1096 pKa = 9.82TVQAGDD1102 pKa = 3.61NDD1104 pKa = 3.9ADD1106 pKa = 4.25GIVVNGISLNGGTIRR1121 pKa = 11.84DD1122 pKa = 3.81GAGNNANLSLTGHH1135 pKa = 6.73IPATTGVLVDD1145 pKa = 3.9TAAPSFTSATVNGATLVMTYY1165 pKa = 10.68SDD1167 pKa = 5.24AGLLDD1172 pKa = 4.67AGHH1175 pKa = 7.13APGAGAFTVMVGGAPVSVTAVAVNGAAHH1203 pKa = 5.87TVSLTLASPVAAGAAVTVAYY1223 pKa = 10.43VDD1225 pKa = 3.75PTAGDD1230 pKa = 3.87DD1231 pKa = 3.52VSAIQDD1237 pKa = 3.35AGRR1240 pKa = 11.84NDD1242 pKa = 3.97AASLAATSVTNTTAAPPTTPPTTPPVTTTVDD1273 pKa = 3.54GVPVQTQTSTASDD1286 pKa = 3.69GSVSQIVTVLVVQPGRR1302 pKa = 11.84VEE1304 pKa = 3.8QVGNNTVADD1313 pKa = 3.73IPLVKK1318 pKa = 10.58DD1319 pKa = 3.54GAGQALLALQVPVGYY1334 pKa = 10.49GLQASGAAQPKK1345 pKa = 9.58AAGDD1349 pKa = 3.83ALSDD1353 pKa = 4.37LIRR1356 pKa = 11.84EE1357 pKa = 4.32IRR1359 pKa = 11.84AHH1361 pKa = 5.96TNSSSQDD1368 pKa = 3.18QGQLGGAGTDD1378 pKa = 3.86FVNGLGASTPLLVQTIVTTGADD1400 pKa = 3.91AAPGAQPLTISGQPQAQGQPQTALVIDD1427 pKa = 4.34GRR1429 pKa = 11.84SNPGIHH1435 pKa = 7.08IEE1437 pKa = 4.04LQNVEE1442 pKa = 4.25FAAIIGSATVTGGAGSQHH1460 pKa = 5.14VWGDD1464 pKa = 3.54GASQTIFLGADD1475 pKa = 3.32DD1476 pKa = 5.41DD1477 pKa = 4.36VLHH1480 pKa = 6.94GGGGNDD1486 pKa = 3.44TVGSAGGNDD1495 pKa = 3.56EE1496 pKa = 5.13VYY1498 pKa = 10.99GDD1500 pKa = 4.78DD1501 pKa = 4.01GDD1503 pKa = 4.27DD1504 pKa = 3.49VVVGGEE1510 pKa = 4.08GADD1513 pKa = 3.61FVHH1516 pKa = 6.95GNAGNDD1522 pKa = 3.63TARR1525 pKa = 11.84GDD1527 pKa = 3.66AGDD1530 pKa = 3.93DD1531 pKa = 3.37LVYY1534 pKa = 10.69GGKK1537 pKa = 10.53GDD1539 pKa = 3.78DD1540 pKa = 3.69QVFGGADD1547 pKa = 3.49NDD1549 pKa = 4.65LLFGDD1554 pKa = 5.1LGQDD1558 pKa = 3.33TLQGNTGHH1566 pKa = 6.97DD1567 pKa = 3.87TLDD1570 pKa = 3.73GGADD1574 pKa = 3.53NDD1576 pKa = 5.23LMFGGQNNDD1585 pKa = 3.42LLLGGEE1591 pKa = 4.35GDD1593 pKa = 4.27DD1594 pKa = 5.16RR1595 pKa = 11.84LNGDD1599 pKa = 4.76LGNDD1603 pKa = 3.63TLQGNVGRR1611 pKa = 11.84DD1612 pKa = 3.56TLEE1615 pKa = 4.31GGAGADD1621 pKa = 3.78FLYY1624 pKa = 10.64GGQDD1628 pKa = 3.18NDD1630 pKa = 3.64VLSGGEE1636 pKa = 4.08GADD1639 pKa = 3.28TLFGDD1644 pKa = 4.86RR1645 pKa = 11.84GDD1647 pKa = 3.76DD1648 pKa = 3.67TLIGGAGADD1657 pKa = 3.68LFVTASGRR1665 pKa = 11.84GEE1667 pKa = 4.13DD1668 pKa = 3.91QVLDD1672 pKa = 4.55FAAGDD1677 pKa = 3.85RR1678 pKa = 11.84VGLLAGTTYY1687 pKa = 10.92SAAQVGGDD1695 pKa = 3.55TVITLTGGGQTTLVNVQLSGLGDD1718 pKa = 3.37GWITTII1724 pKa = 4.94

Molecular weight: 169.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7D7K4|A0A0Q7D7K4_9CAUL Uncharacterized protein OS=Phenylobacterium sp. Root1277 OX=1736442 GN=ASC73_14245 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGAKK29 pKa = 9.48IVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4022

0

4022

1269598

41

3085

315.7

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.998 ± 0.055

0.731 ± 0.013

5.754 ± 0.026

5.7 ± 0.038

3.626 ± 0.023

9.034 ± 0.038

1.804 ± 0.019

4.411 ± 0.028

3.171 ± 0.031

9.977 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.018

2.353 ± 0.026

5.445 ± 0.034

3.23 ± 0.022

7.107 ± 0.038

5.086 ± 0.024

5.076 ± 0.03

7.542 ± 0.028

1.41 ± 0.017

2.203 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski