Anelloviridae sp.
Average proteome isoelectric point is 8.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385E3J9|A0A385E3J9_9VIRU Capsid protein OS=Anelloviridae sp. OX=2055263 PE=3 SV=1
MM1 pKa = 7.51 GKK3 pKa = 9.86 ALKK6 pKa = 10.31 KK7 pKa = 11.01 AMFLGKK13 pKa = 10.33 LYY15 pKa = 10.33 RR16 pKa = 11.84 KK17 pKa = 9.49 KK18 pKa = 10.54 RR19 pKa = 11.84 RR20 pKa = 11.84 LSLSSLHH27 pKa = 6.43 CAPKK31 pKa = 10.29 ACKK34 pKa = 9.59 LLKK37 pKa = 10.73 GMWRR41 pKa = 11.84 PPTQNVSGQEE51 pKa = 4.14 RR52 pKa = 11.84 NWYY55 pKa = 9.37 DD56 pKa = 3.14 AVFYY60 pKa = 10.96 SHH62 pKa = 7.26 AAFCGCGDD70 pKa = 4.1 CVGHH74 pKa = 7.09 LSYY77 pKa = 11.07 LATHH81 pKa = 7.37 LGRR84 pKa = 11.84 PPSAQPPPQPQPPVIRR100 pKa = 11.84 RR101 pKa = 11.84 LPALPAPPNPSGDD114 pKa = 3.18 RR115 pKa = 11.84 AAWPTGGGDD124 pKa = 3.56 AGEE127 pKa = 5.05 DD128 pKa = 4.04 GPGEE132 pKa = 4.23 GGAAGGFADD141 pKa = 5.39 LADD144 pKa = 5.22 DD145 pKa = 4.47 EE146 pKa = 5.37 LLTAAVEE153 pKa = 4.29 AAEE156 pKa = 4.21 EE157 pKa = 4.1
Molecular weight: 16.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.405
IPC2_protein 7.644
IPC_protein 7.614
Toseland 7.205
ProMoST 8.039
Dawson 8.258
Bjellqvist 8.624
Wikipedia 8.17
Rodwell 8.258
Grimsley 7.19
Solomon 8.404
Lehninger 8.434
Nozaki 8.931
DTASelect 8.346
Thurlkill 8.404
EMBOSS 8.492
Sillero 8.756
Patrickios 4.418
IPC_peptide 8.404
IPC2_peptide 7.761
IPC2.peptide.svr19 7.733
Protein with the highest isoelectric point:
>tr|A0A385E6L0|A0A385E6L0_9VIRU Uncharacterized protein OS=Anelloviridae sp. OX=2055263 PE=4 SV=1
MM1 pKa = 7.64 AYY3 pKa = 8.26 WWWRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 PWRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 WRR21 pKa = 11.84 LRR23 pKa = 11.84 RR24 pKa = 11.84 PRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 APYY32 pKa = 8.44 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 GRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 VRR42 pKa = 11.84 KK43 pKa = 9.44 RR44 pKa = 11.84 RR45 pKa = 11.84 GRR47 pKa = 11.84 WRR49 pKa = 11.84 RR50 pKa = 11.84 TYY52 pKa = 10.49 RR53 pKa = 11.84 RR54 pKa = 11.84 WRR56 pKa = 11.84 RR57 pKa = 11.84 KK58 pKa = 8.68 RR59 pKa = 11.84 GRR61 pKa = 11.84 RR62 pKa = 11.84 KK63 pKa = 9.46 HH64 pKa = 5.55 RR65 pKa = 11.84 PKK67 pKa = 10.4 IILKK71 pKa = 8.59 QWQPNFIRR79 pKa = 11.84 NCFIVGYY86 pKa = 8.81 MPLIFCGEE94 pKa = 4.06 NTVSQNYY101 pKa = 7.24 ATHH104 pKa = 6.89 SDD106 pKa = 3.67 DD107 pKa = 5.47 LVAKK111 pKa = 9.65 GPYY114 pKa = 8.76 GGGMTTTKK122 pKa = 9.14 FTLRR126 pKa = 11.84 ILFDD130 pKa = 3.04 EE131 pKa = 4.62 HH132 pKa = 7.5 LRR134 pKa = 11.84 YY135 pKa = 9.03 MNYY138 pKa = 7.2 WTVSNEE144 pKa = 3.71 DD145 pKa = 3.73 LDD147 pKa = 3.82 LCRR150 pKa = 11.84 YY151 pKa = 9.35 VGAKK155 pKa = 9.93 FYY157 pKa = 10.17 FYY159 pKa = 10.81 RR160 pKa = 11.84 HH161 pKa = 5.45 PTVDD165 pKa = 4.17 FMVQIHH171 pKa = 5.41 TQAPFLDD178 pKa = 4.23 TEE180 pKa = 4.33 LTGPSIHH187 pKa = 7.18 PGMMILNKK195 pKa = 10.2 RR196 pKa = 11.84 KK197 pKa = 9.93 IFVPSLKK204 pKa = 9.28 TRR206 pKa = 11.84 PNRR209 pKa = 11.84 KK210 pKa = 8.15 HH211 pKa = 5.71 KK212 pKa = 10.84 VRR214 pKa = 11.84 VKK216 pKa = 10.46 VGAPRR221 pKa = 11.84 LFEE224 pKa = 5.01 DD225 pKa = 2.54 KK226 pKa = 10.04 WYY228 pKa = 8.92 PQSEE232 pKa = 4.62 LCDD235 pKa = 3.6 TTLVAIYY242 pKa = 8.26 ATACDD247 pKa = 3.79 LQFPFGSPLTEE258 pKa = 3.84 NPCVNFQVLGSAYY271 pKa = 8.94 KK272 pKa = 9.51 QHH274 pKa = 6.97 LSILTTEE281 pKa = 4.14 TEE283 pKa = 4.21 KK284 pKa = 11.41 NKK286 pKa = 10.42 AHH288 pKa = 6.5 YY289 pKa = 8.99 EE290 pKa = 3.68 QALFNKK296 pKa = 8.35 VAYY299 pKa = 10.17 YY300 pKa = 9.1 NTFQTIAHH308 pKa = 6.05 LRR310 pKa = 11.84 EE311 pKa = 3.98 TGKK314 pKa = 10.02 QEE316 pKa = 3.82 QANPQWSTCVNSTALKK332 pKa = 10.48 FDD334 pKa = 3.7 GTNQNQCNDD343 pKa = 2.33 TWYY346 pKa = 11.0 YY347 pKa = 10.12 GNAYY351 pKa = 9.47 NCNIQSLCEE360 pKa = 3.97 KK361 pKa = 9.77 TRR363 pKa = 11.84 QKK365 pKa = 10.92 YY366 pKa = 9.45 FKK368 pKa = 8.83 ATKK371 pKa = 10.01 SAFPAYY377 pKa = 8.32 ATLVDD382 pKa = 5.31 DD383 pKa = 4.86 LYY385 pKa = 10.26 EE386 pKa = 4.11 YY387 pKa = 10.85 HH388 pKa = 6.81 GGIYY392 pKa = 10.33 SSIFLSAGRR401 pKa = 11.84 SYY403 pKa = 11.41 FEE405 pKa = 3.91 TLGAYY410 pKa = 10.15 SDD412 pKa = 4.3 IIYY415 pKa = 10.87 NPFVDD420 pKa = 4.42 KK421 pKa = 11.26 GLGNIVWIDD430 pKa = 3.67 YY431 pKa = 10.16 LSKK434 pKa = 10.86 SDD436 pKa = 5.01 AVWKK440 pKa = 9.62 PPQSKK445 pKa = 8.33 CTIKK449 pKa = 10.72 DD450 pKa = 3.37 QPLWAAFNGYY460 pKa = 9.26 SEE462 pKa = 5.38 FCAKK466 pKa = 9.43 STGDD470 pKa = 3.2 GAVNINCRR478 pKa = 11.84 VVVRR482 pKa = 11.84 CPYY485 pKa = 5.83 TTPMLIDD492 pKa = 3.91 NKK494 pKa = 10.79 DD495 pKa = 3.55 PNQGFVPYY503 pKa = 10.41 SKK505 pKa = 11.04 NFGNAKK511 pKa = 9.17 MKK513 pKa = 10.58 GGSADD518 pKa = 3.48 VPLSWRR524 pKa = 11.84 VKK526 pKa = 8.01 WYY528 pKa = 11.04 VMMYY532 pKa = 9.13 HH533 pKa = 5.21 QQEE536 pKa = 4.12 FMEE539 pKa = 5.36 EE540 pKa = 4.1 IVQSGPWAYY549 pKa = 9.95 KK550 pKa = 10.45 GDD552 pKa = 3.94 QKK554 pKa = 11.33 SAALNMKK561 pKa = 9.88 YY562 pKa = 9.92 VFKK565 pKa = 10.26 WKK567 pKa = 9.49 WGGNPISGQVVRR579 pKa = 11.84 NPCKK583 pKa = 9.14 TSGSSRR589 pKa = 11.84 EE590 pKa = 3.89 PRR592 pKa = 11.84 SIQAVDD598 pKa = 4.09 PKK600 pKa = 10.55 RR601 pKa = 11.84 VAPPLVFHH609 pKa = 6.36 SWDD612 pKa = 3.15 YY613 pKa = 9.77 RR614 pKa = 11.84 RR615 pKa = 11.84 GYY617 pKa = 9.93 FGHH620 pKa = 7.27 ASIKK624 pKa = 10.35 RR625 pKa = 11.84 MSEE628 pKa = 3.68 EE629 pKa = 4.34 SEE631 pKa = 4.21 PPTLFTGPSGKK642 pKa = 9.81 RR643 pKa = 11.84 PRR645 pKa = 11.84 RR646 pKa = 11.84 DD647 pKa = 3.15 TNVQQAEE654 pKa = 4.23 EE655 pKa = 4.17 EE656 pKa = 4.24 QQKK659 pKa = 10.34 EE660 pKa = 4.11 SSTFRR665 pKa = 11.84 VQQQLQPWINSSQEE679 pKa = 4.11 TQSSQEE685 pKa = 4.07 EE686 pKa = 4.08 IQAQGSIQEE695 pKa = 4.15 QLLQQLGEE703 pKa = 3.9 QRR705 pKa = 11.84 ALRR708 pKa = 11.84 KK709 pKa = 9.49 QLEE712 pKa = 4.3 HH713 pKa = 6.71 VARR716 pKa = 11.84 QVLKK720 pKa = 10.52 VQAGTHH726 pKa = 5.72 LHH728 pKa = 6.75 PLLSSQAA735 pKa = 3.44
Molecular weight: 86.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.271
IPC2_protein 9.487
IPC_protein 9.663
Toseland 10.014
ProMoST 9.78
Dawson 10.262
Bjellqvist 9.97
Wikipedia 10.452
Rodwell 10.57
Grimsley 10.35
Solomon 10.277
Lehninger 10.233
Nozaki 10.058
DTASelect 9.955
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.175
Patrickios 9.75
IPC_peptide 10.277
IPC2_peptide 8.858
IPC2.peptide.svr19 8.413
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1072
157
735
357.3
40.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.09 ± 1.812
2.052 ± 0.329
3.638 ± 0.465
4.664 ± 0.284
3.545 ± 1.004
6.437 ± 1.436
2.239 ± 0.174
2.985 ± 1.291
7.09 ± 1.068
7.183 ± 0.961
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.679 ± 0.361
4.291 ± 0.646
6.81 ± 1.383
5.877 ± 0.94
8.769 ± 0.835
8.116 ± 3.021
5.504 ± 0.757
4.944 ± 0.741
2.425 ± 0.725
4.664 ± 0.635
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here