Betta splendens (Siamese fighting fish)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata; Acanthomorphata;

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41292 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P7LUX0|A0A6P7LUX0_BETSP transcription factor E2F6-like OS=Betta splendens OX=158456 GN=LOC114850007 PE=3 SV=1
MM1 pKa = 7.9DD2 pKa = 5.47LSFMAAQIPVMTGAFMDD19 pKa = 4.02SSPNDD24 pKa = 3.88DD25 pKa = 3.69YY26 pKa = 11.86STDD29 pKa = 3.18HH30 pKa = 6.97SLFNSSASVHH40 pKa = 5.71AASMVAQSPPEE51 pKa = 4.19EE52 pKa = 4.27PQSMSRR58 pKa = 11.84DD59 pKa = 4.1AIWLWIAITATIGNIVVVGVVYY81 pKa = 10.98AFTFF85 pKa = 3.7

Molecular weight: 9.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P7NV97|A0A6P7NV97_BETSP Isoform of A0A6P7P2G4 tuftelin-like isoform X4 OS=Betta splendens OX=158456 GN=LOC114866266 PE=4 SV=1
MM1 pKa = 7.76AFMSAKK7 pKa = 10.31LEE9 pKa = 4.09RR10 pKa = 11.84SGVWSVPAEE19 pKa = 3.78QVSTNRR25 pKa = 11.84SASTGQRR32 pKa = 11.84PQVSTNRR39 pKa = 11.84SASTGQRR46 pKa = 11.84LQVSANRR53 pKa = 11.84STPTGQRR60 pKa = 11.84QQVSANRR67 pKa = 11.84SAPTGQRR74 pKa = 11.84QQVNASRR81 pKa = 11.84SAPTGQRR88 pKa = 11.84PQVSANRR95 pKa = 11.84SAPTGQRR102 pKa = 11.84QQVNASRR109 pKa = 11.84SAPTGQRR116 pKa = 11.84QQVNASRR123 pKa = 11.84SAPTGQRR130 pKa = 11.84QQVSAHH136 pKa = 6.38RR137 pKa = 11.84SASTGQRR144 pKa = 11.84PQVNANRR151 pKa = 11.84STPRR155 pKa = 11.84VNAHH159 pKa = 6.23RR160 pKa = 11.84SVPTGQRR167 pKa = 11.84PQVNAHH173 pKa = 6.75RR174 pKa = 11.84STPTGQRR181 pKa = 11.84PQVNANRR188 pKa = 11.84STPRR192 pKa = 11.84VNAHH196 pKa = 6.23RR197 pKa = 11.84SVPTGQRR204 pKa = 11.84PP205 pKa = 3.04

Molecular weight: 21.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22601

18691

41292

32944339

31

33161

797.8

88.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.935 ± 0.01

2.089 ± 0.011

5.286 ± 0.008

7.023 ± 0.018

3.273 ± 0.009

6.379 ± 0.016

2.663 ± 0.006

4.033 ± 0.009

5.541 ± 0.016

9.378 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.006

3.688 ± 0.007

5.97 ± 0.02

4.966 ± 0.013

5.84 ± 0.009

9.064 ± 0.015

5.695 ± 0.013

6.293 ± 0.011

1.079 ± 0.004

2.548 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski