Amino acid dipepetide frequency for Enterococcus phage phiEF24C (Enterococcus bacteriophage phi-EF24C)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.166AlaAla: 0.166 ± 0.073
0.403AlaCys: 0.403 ± 0.11
3.41AlaAsp: 3.41 ± 0.357
4.475AlaGlu: 4.475 ± 0.387
2.178AlaPhe: 2.178 ± 0.209
3.173AlaGly: 3.173 ± 0.443
0.71AlaHis: 0.71 ± 0.137
4.167AlaIle: 4.167 ± 0.328
5.493AlaLys: 5.493 ± 0.365
5.138AlaLeu: 5.138 ± 0.326
1.657AlaMet: 1.657 ± 0.258
3.126AlaAsn: 3.126 ± 0.347
1.989AlaPro: 1.989 ± 0.256
2.368AlaGln: 2.368 ± 0.279
2.178AlaArg: 2.178 ± 0.22
3.386AlaSer: 3.386 ± 0.37
3.789AlaThr: 3.789 ± 0.322
4.073AlaVal: 4.073 ± 0.303
0.592AlaTrp: 0.592 ± 0.105
2.368AlaTyr: 2.368 ± 0.235
0.0AlaXaa: 0.0 ± 0.0
Cys
0.426CysAla: 0.426 ± 0.11
0.071CysCys: 0.071 ± 0.044
0.521CysAsp: 0.521 ± 0.135
0.568CysGlu: 0.568 ± 0.125
0.379CysPhe: 0.379 ± 0.1
0.805CysGly: 0.805 ± 0.16
0.142CysHis: 0.142 ± 0.069
0.284CysIle: 0.284 ± 0.09
0.474CysLys: 0.474 ± 0.108
0.758CysLeu: 0.758 ± 0.118
0.095CysMet: 0.095 ± 0.053
0.331CysAsn: 0.331 ± 0.093
0.379CysPro: 0.379 ± 0.107
0.118CysGln: 0.118 ± 0.057
0.26CysArg: 0.26 ± 0.078
0.45CysSer: 0.45 ± 0.112
0.497CysThr: 0.497 ± 0.119
0.568CysVal: 0.568 ± 0.113
0.095CysTrp: 0.095 ± 0.048
0.545CysTyr: 0.545 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
3.054AspAla: 3.054 ± 0.336
0.568AspCys: 0.568 ± 0.139
2.723AspAsp: 2.723 ± 0.294
5.517AspGlu: 5.517 ± 0.387
2.581AspPhe: 2.581 ± 0.23
3.789AspGly: 3.789 ± 0.4
0.521AspHis: 0.521 ± 0.116
4.901AspIle: 4.901 ± 0.31
5.47AspLys: 5.47 ± 0.374
4.925AspLeu: 4.925 ± 0.336
2.084AspMet: 2.084 ± 0.27
3.717AspAsn: 3.717 ± 0.306
1.35AspPro: 1.35 ± 0.21
0.994AspGln: 0.994 ± 0.162
2.202AspArg: 2.202 ± 0.224
3.907AspSer: 3.907 ± 0.324
4.38AspThr: 4.38 ± 0.315
4.759AspVal: 4.759 ± 0.355
0.852AspTrp: 0.852 ± 0.138
3.386AspTyr: 3.386 ± 0.31
0.0AspXaa: 0.0 ± 0.0
Glu
5.257GluAla: 5.257 ± 0.382
0.781GluCys: 0.781 ± 0.132
5.114GluAsp: 5.114 ± 0.346
10.016GluGlu: 10.016 ± 0.816
2.983GluPhe: 2.983 ± 0.271
5.043GluGly: 5.043 ± 0.333
1.444GluHis: 1.444 ± 0.218
5.28GluIle: 5.28 ± 0.327
6.511GluLys: 6.511 ± 0.458
9.519GluLeu: 9.519 ± 0.616
1.918GluMet: 1.918 ± 0.225
4.688GluAsn: 4.688 ± 0.372
2.652GluPro: 2.652 ± 0.268
4.096GluGln: 4.096 ± 0.332
3.362GluArg: 3.362 ± 0.283
4.475GluSer: 4.475 ± 0.353
4.002GluThr: 4.002 ± 0.273
6.488GluVal: 6.488 ± 0.447
1.018GluTrp: 1.018 ± 0.152
3.789GluTyr: 3.789 ± 0.276
0.0GluXaa: 0.0 ± 0.0
Phe
1.705PheAla: 1.705 ± 0.191
0.331PheCys: 0.331 ± 0.09
2.391PheAsp: 2.391 ± 0.273
2.486PheGlu: 2.486 ± 0.266
1.326PhePhe: 1.326 ± 0.191
2.107PheGly: 2.107 ± 0.268
0.474PheHis: 0.474 ± 0.127
3.102PheIle: 3.102 ± 0.297
2.723PheLys: 2.723 ± 0.274
2.96PheLeu: 2.96 ± 0.312
0.947PheMet: 0.947 ± 0.139
2.605PheAsn: 2.605 ± 0.221
1.089PhePro: 1.089 ± 0.197
1.089PheGln: 1.089 ± 0.132
1.35PheArg: 1.35 ± 0.167
3.315PheSer: 3.315 ± 0.273
3.007PheThr: 3.007 ± 0.29
2.391PheVal: 2.391 ± 0.252
0.331PheTrp: 0.331 ± 0.088
1.776PheTyr: 1.776 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
3.646GlyAla: 3.646 ± 0.467
0.521GlyCys: 0.521 ± 0.106
3.717GlyAsp: 3.717 ± 0.332
4.523GlyGlu: 4.523 ± 0.3
2.297GlyPhe: 2.297 ± 0.195
4.712GlyGly: 4.712 ± 0.593
1.066GlyHis: 1.066 ± 0.161
4.475GlyIle: 4.475 ± 0.368
5.28GlyLys: 5.28 ± 0.328
4.523GlyLeu: 4.523 ± 0.267
1.823GlyMet: 1.823 ± 0.222
3.244GlyAsn: 3.244 ± 0.278
0.024GlyPro: 0.024 ± 0.024
1.871GlyGln: 1.871 ± 0.246
2.486GlyArg: 2.486 ± 0.25
4.002GlySer: 4.002 ± 0.381
4.546GlyThr: 4.546 ± 0.402
4.57GlyVal: 4.57 ± 0.332
0.994GlyTrp: 0.994 ± 0.144
3.765GlyTyr: 3.765 ± 0.291
0.0GlyXaa: 0.0 ± 0.0
His
0.758HisAla: 0.758 ± 0.124
0.308HisCys: 0.308 ± 0.097
0.947HisAsp: 0.947 ± 0.144
0.829HisGlu: 0.829 ± 0.118
0.497HisPhe: 0.497 ± 0.108
0.9HisGly: 0.9 ± 0.156
0.237HisHis: 0.237 ± 0.081
1.16HisIle: 1.16 ± 0.204
1.113HisLys: 1.113 ± 0.175
1.137HisLeu: 1.137 ± 0.201
0.284HisMet: 0.284 ± 0.078
0.971HisAsn: 0.971 ± 0.167
0.568HisPro: 0.568 ± 0.098
0.379HisGln: 0.379 ± 0.088
0.521HisArg: 0.521 ± 0.103
0.781HisSer: 0.781 ± 0.145
0.994HisThr: 0.994 ± 0.157
1.184HisVal: 1.184 ± 0.171
0.26HisTrp: 0.26 ± 0.085
0.9HisTyr: 0.9 ± 0.133
0.0HisXaa: 0.0 ± 0.0
Ile
3.812IleAla: 3.812 ± 0.349
0.355IleCys: 0.355 ± 0.083
4.949IleAsp: 4.949 ± 0.318
6.133IleGlu: 6.133 ± 0.427
2.036IlePhe: 2.036 ± 0.254
3.504IleGly: 3.504 ± 0.34
1.113IleHis: 1.113 ± 0.188
4.783IleIle: 4.783 ± 0.419
5.706IleLys: 5.706 ± 0.359
4.688IleLeu: 4.688 ± 0.345
1.634IleMet: 1.634 ± 0.194
4.451IleAsn: 4.451 ± 0.372
2.036IlePro: 2.036 ± 0.234
2.439IleGln: 2.439 ± 0.265
2.912IleArg: 2.912 ± 0.226
4.617IleSer: 4.617 ± 0.289
3.931IleThr: 3.931 ± 0.345
4.144IleVal: 4.144 ± 0.43
0.497IleTrp: 0.497 ± 0.11
2.605IleTyr: 2.605 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
4.38LysAla: 4.38 ± 0.373
0.616LysCys: 0.616 ± 0.133
4.925LysAsp: 4.925 ± 0.36
9.708LysGlu: 9.708 ± 0.457
2.391LysPhe: 2.391 ± 0.251
4.191LysGly: 4.191 ± 0.296
1.279LysHis: 1.279 ± 0.18
4.286LysIle: 4.286 ± 0.278
5.991LysLys: 5.991 ± 0.376
6.867LysLeu: 6.867 ± 0.35
2.202LysMet: 2.202 ± 0.226
4.333LysAsn: 4.333 ± 0.295
2.747LysPro: 2.747 ± 0.271
3.197LysGln: 3.197 ± 0.224
3.836LysArg: 3.836 ± 0.287
3.836LysSer: 3.836 ± 0.318
4.309LysThr: 4.309 ± 0.31
6.275LysVal: 6.275 ± 0.395
0.71LysTrp: 0.71 ± 0.122
3.126LysTyr: 3.126 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
5.422LeuAla: 5.422 ± 0.368
0.474LeuCys: 0.474 ± 0.118
6.18LeuAsp: 6.18 ± 0.39
8.429LeuGlu: 8.429 ± 0.584
3.054LeuPhe: 3.054 ± 0.303
6.227LeuGly: 6.227 ± 0.375
0.971LeuHis: 0.971 ± 0.155
5.02LeuIle: 5.02 ± 0.351
6.298LeuLys: 6.298 ± 0.409
7.932LeuLeu: 7.932 ± 0.56
2.226LeuMet: 2.226 ± 0.228
5.754LeuAsn: 5.754 ± 0.466
2.652LeuPro: 2.652 ± 0.283
3.362LeuGln: 3.362 ± 0.279
3.552LeuArg: 3.552 ± 0.248
5.47LeuSer: 5.47 ± 0.335
5.328LeuThr: 5.328 ± 0.366
4.428LeuVal: 4.428 ± 0.394
0.639LeuTrp: 0.639 ± 0.145
3.197LeuTyr: 3.197 ± 0.33
0.0LeuXaa: 0.0 ± 0.0
Met
1.729MetAla: 1.729 ± 0.235
0.166MetCys: 0.166 ± 0.066
1.515MetAsp: 1.515 ± 0.186
1.847MetGlu: 1.847 ± 0.193
0.876MetPhe: 0.876 ± 0.127
1.563MetGly: 1.563 ± 0.272
0.379MetHis: 0.379 ± 0.089
1.492MetIle: 1.492 ± 0.16
2.439MetLys: 2.439 ± 0.213
2.391MetLeu: 2.391 ± 0.221
0.45MetMet: 0.45 ± 0.107
1.942MetAsn: 1.942 ± 0.2
0.663MetPro: 0.663 ± 0.149
0.876MetGln: 0.876 ± 0.185
1.089MetArg: 1.089 ± 0.154
1.894MetSer: 1.894 ± 0.167
1.752MetThr: 1.752 ± 0.186
1.137MetVal: 1.137 ± 0.162
0.166MetTrp: 0.166 ± 0.058
1.184MetTyr: 1.184 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
2.818AsnAla: 2.818 ± 0.268
0.331AsnCys: 0.331 ± 0.092
3.126AsnAsp: 3.126 ± 0.271
4.475AsnGlu: 4.475 ± 0.403
1.965AsnPhe: 1.965 ± 0.234
4.925AsnGly: 4.925 ± 0.336
1.231AsnHis: 1.231 ± 0.169
3.504AsnIle: 3.504 ± 0.246
5.399AsnLys: 5.399 ± 0.353
4.451AsnLeu: 4.451 ± 0.313
1.492AsnMet: 1.492 ± 0.179
3.931AsnAsn: 3.931 ± 0.41
1.942AsnPro: 1.942 ± 0.225
1.942AsnGln: 1.942 ± 0.271
2.391AsnArg: 2.391 ± 0.243
3.765AsnSer: 3.765 ± 0.328
3.339AsnThr: 3.339 ± 0.298
3.67AsnVal: 3.67 ± 0.294
0.687AsnTrp: 0.687 ± 0.132
2.747AsnTyr: 2.747 ± 0.277
0.0AsnXaa: 0.0 ± 0.0
Pro
1.444ProAla: 1.444 ± 0.254
0.237ProCys: 0.237 ± 0.069
2.178ProAsp: 2.178 ± 0.246
3.007ProGlu: 3.007 ± 0.286
1.326ProPhe: 1.326 ± 0.188
0.521ProGly: 0.521 ± 0.111
0.474ProHis: 0.474 ± 0.105
2.107ProIle: 2.107 ± 0.253
2.226ProLys: 2.226 ± 0.267
2.344ProLeu: 2.344 ± 0.252
0.687ProMet: 0.687 ± 0.136
1.657ProAsn: 1.657 ± 0.218
0.568ProPro: 0.568 ± 0.116
1.042ProGln: 1.042 ± 0.251
1.113ProArg: 1.113 ± 0.154
2.178ProSer: 2.178 ± 0.271
2.249ProThr: 2.249 ± 0.209
2.534ProVal: 2.534 ± 0.319
0.379ProTrp: 0.379 ± 0.094
1.563ProTyr: 1.563 ± 0.181
0.0ProXaa: 0.0 ± 0.0
Gln
2.865GlnAla: 2.865 ± 0.303
0.142GlnCys: 0.142 ± 0.059
1.492GlnAsp: 1.492 ± 0.185
2.912GlnGlu: 2.912 ± 0.271
1.539GlnPhe: 1.539 ± 0.202
2.202GlnGly: 2.202 ± 0.258
0.355GlnHis: 0.355 ± 0.084
1.847GlnIle: 1.847 ± 0.2
2.249GlnLys: 2.249 ± 0.278
3.268GlnLeu: 3.268 ± 0.239
0.876GlnMet: 0.876 ± 0.172
1.468GlnAsn: 1.468 ± 0.198
1.563GlnPro: 1.563 ± 0.448
1.8GlnGln: 1.8 ± 0.471
1.397GlnArg: 1.397 ± 0.199
2.036GlnSer: 2.036 ± 0.231
1.61GlnThr: 1.61 ± 0.185
2.699GlnVal: 2.699 ± 0.258
0.308GlnTrp: 0.308 ± 0.103
1.681GlnTyr: 1.681 ± 0.205
0.0GlnXaa: 0.0 ± 0.0
Arg
2.463ArgAla: 2.463 ± 0.338
0.331ArgCys: 0.331 ± 0.097
2.439ArgAsp: 2.439 ± 0.298
3.646ArgGlu: 3.646 ± 0.29
1.776ArgPhe: 1.776 ± 0.183
2.391ArgGly: 2.391 ± 0.274
0.592ArgHis: 0.592 ± 0.117
2.652ArgIle: 2.652 ± 0.256
3.197ArgLys: 3.197 ± 0.288
3.836ArgLeu: 3.836 ± 0.306
0.994ArgMet: 0.994 ± 0.153
1.942ArgAsn: 1.942 ± 0.228
1.042ArgPro: 1.042 ± 0.152
1.468ArgGln: 1.468 ± 0.21
1.468ArgArg: 1.468 ± 0.174
1.989ArgSer: 1.989 ± 0.215
2.178ArgThr: 2.178 ± 0.226
3.268ArgVal: 3.268 ± 0.32
0.331ArgTrp: 0.331 ± 0.094
1.989ArgTyr: 1.989 ± 0.221
0.0ArgXaa: 0.0 ± 0.0
Ser
3.197SerAla: 3.197 ± 0.285
0.474SerCys: 0.474 ± 0.116
4.167SerAsp: 4.167 ± 0.369
4.096SerGlu: 4.096 ± 0.295
2.699SerPhe: 2.699 ± 0.283
4.025SerGly: 4.025 ± 0.384
0.994SerHis: 0.994 ± 0.157
4.523SerIle: 4.523 ± 0.321
5.209SerLys: 5.209 ± 0.401
5.422SerLeu: 5.422 ± 0.352
1.776SerMet: 1.776 ± 0.182
3.173SerAsn: 3.173 ± 0.257
1.776SerPro: 1.776 ± 0.205
2.226SerGln: 2.226 ± 0.258
2.415SerArg: 2.415 ± 0.268
4.309SerSer: 4.309 ± 0.312
3.646SerThr: 3.646 ± 0.406
4.286SerVal: 4.286 ± 0.275
0.781SerTrp: 0.781 ± 0.148
2.794SerTyr: 2.794 ± 0.25
0.0SerXaa: 0.0 ± 0.0
Thr
3.765ThrAla: 3.765 ± 0.321
0.45ThrCys: 0.45 ± 0.116
3.575ThrAsp: 3.575 ± 0.31
5.114ThrGlu: 5.114 ± 0.416
2.652ThrPhe: 2.652 ± 0.245
3.907ThrGly: 3.907 ± 0.326
0.805ThrHis: 0.805 ± 0.119
4.854ThrIle: 4.854 ± 0.366
4.428ThrLys: 4.428 ± 0.369
5.801ThrLeu: 5.801 ± 0.382
1.35ThrMet: 1.35 ± 0.183
3.102ThrAsn: 3.102 ± 0.216
2.344ThrPro: 2.344 ± 0.269
1.823ThrGln: 1.823 ± 0.227
2.391ThrArg: 2.391 ± 0.24
3.789ThrSer: 3.789 ± 0.327
4.12ThrThr: 4.12 ± 0.404
4.665ThrVal: 4.665 ± 0.454
0.758ThrTrp: 0.758 ± 0.13
3.197ThrTyr: 3.197 ± 0.306
0.0ThrXaa: 0.0 ± 0.0
Val
4.499ValAla: 4.499 ± 0.343
0.663ValCys: 0.663 ± 0.13
4.854ValAsp: 4.854 ± 0.4
6.014ValGlu: 6.014 ± 0.481
2.983ValPhe: 2.983 ± 0.275
4.144ValGly: 4.144 ± 0.337
1.018ValHis: 1.018 ± 0.156
4.428ValIle: 4.428 ± 0.294
4.807ValLys: 4.807 ± 0.362
5.588ValLeu: 5.588 ± 0.457
1.705ValMet: 1.705 ± 0.199
4.215ValAsn: 4.215 ± 0.358
2.51ValPro: 2.51 ± 0.212
1.586ValGln: 1.586 ± 0.237
2.912ValArg: 2.912 ± 0.306
4.665ValSer: 4.665 ± 0.304
4.972ValThr: 4.972 ± 0.437
5.28ValVal: 5.28 ± 0.449
0.616ValTrp: 0.616 ± 0.122
3.102ValTyr: 3.102 ± 0.25
0.0ValXaa: 0.0 ± 0.0
Trp
0.663TrpAla: 0.663 ± 0.122
0.095TrpCys: 0.095 ± 0.042
0.568TrpAsp: 0.568 ± 0.117
0.994TrpGlu: 0.994 ± 0.141
0.521TrpPhe: 0.521 ± 0.097
0.781TrpGly: 0.781 ± 0.135
0.166TrpHis: 0.166 ± 0.057
0.403TrpIle: 0.403 ± 0.099
0.545TrpLys: 0.545 ± 0.096
1.231TrpLeu: 1.231 ± 0.192
0.166TrpMet: 0.166 ± 0.055
0.568TrpAsn: 0.568 ± 0.109
0.0TrpPro: 0.0 ± 0.0
0.45TrpGln: 0.45 ± 0.127
0.403TrpArg: 0.403 ± 0.112
0.497TrpSer: 0.497 ± 0.097
0.923TrpThr: 0.923 ± 0.163
0.805TrpVal: 0.805 ± 0.19
0.331TrpTrp: 0.331 ± 0.097
0.71TrpTyr: 0.71 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.77TyrAla: 2.77 ± 0.285
0.45TyrCys: 0.45 ± 0.097
2.889TyrAsp: 2.889 ± 0.245
3.646TyrGlu: 3.646 ± 0.281
1.444TyrPhe: 1.444 ± 0.209
3.173TyrGly: 3.173 ± 0.276
0.758TyrHis: 0.758 ± 0.125
3.078TyrIle: 3.078 ± 0.269
3.67TyrLys: 3.67 ± 0.337
3.836TyrLeu: 3.836 ± 0.307
1.16TyrMet: 1.16 ± 0.19
2.912TyrAsn: 2.912 ± 0.321
1.942TyrPro: 1.942 ± 0.267
1.184TyrGln: 1.184 ± 0.193
1.752TyrArg: 1.752 ± 0.223
2.628TyrSer: 2.628 ± 0.261
3.315TyrThr: 3.315 ± 0.316
3.339TyrVal: 3.339 ± 0.306
0.474TyrTrp: 0.474 ± 0.123
2.297TyrTyr: 2.297 ± 0.25
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 221 proteins (42234 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski