Listeria phage A500 (Bacteriophage A500)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8ASM6|A8ASM6_BPA50 Gp37 OS=Listeria phage A500 OX=40522 PE=4 SV=1
MM1 pKa = 8.13IIIEE5 pKa = 4.28EE6 pKa = 4.12FKK8 pKa = 10.76EE9 pKa = 3.75YY10 pKa = 10.45TINNKK15 pKa = 9.48NNNFFNQQILYY26 pKa = 9.76KK27 pKa = 10.56FPNNYY32 pKa = 8.6GASVVSGPYY41 pKa = 9.61TYY43 pKa = 11.14GLEE46 pKa = 3.81LAVIFFSNNSDD57 pKa = 4.02DD58 pKa = 4.64DD59 pKa = 3.55WSLVYY64 pKa = 8.51DD65 pKa = 3.84TPVTNDD71 pKa = 3.19VLGHH75 pKa = 6.72LDD77 pKa = 4.25KK78 pKa = 11.33EE79 pKa = 4.58SLKK82 pKa = 10.62QALEE86 pKa = 4.6DD87 pKa = 4.41IYY89 pKa = 11.46NLPIKK94 pKa = 10.53

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8ASN7|A8ASN7_BPA50 Gp48 OS=Listeria phage A500 OX=40522 PE=4 SV=1
MM1 pKa = 7.31FLLGKK6 pKa = 9.4YY7 pKa = 7.82YY8 pKa = 9.89WHH10 pKa = 7.06VSRR13 pKa = 11.84LGGKK17 pKa = 8.95PSEE20 pKa = 3.84IRR22 pKa = 11.84HH23 pKa = 5.51YY24 pKa = 10.98NHH26 pKa = 5.91ITKK29 pKa = 9.45MYY31 pKa = 10.54KK32 pKa = 10.34FILRR36 pKa = 11.84NPAMFKK42 pKa = 10.89DD43 pKa = 3.79KK44 pKa = 10.4TLTIYY49 pKa = 11.07DD50 pKa = 3.48HH51 pKa = 7.07AKK53 pKa = 9.8PVTNMTFNEE62 pKa = 3.38IRR64 pKa = 11.84YY65 pKa = 8.72RR66 pKa = 11.84ASLNLCEE73 pKa = 4.24TVEE76 pKa = 4.09RR77 pKa = 11.84KK78 pKa = 9.52YY79 pKa = 11.49VLGLTEE85 pKa = 5.31RR86 pKa = 11.84LTKK89 pKa = 8.62EE90 pKa = 3.79QKK92 pKa = 10.22GVRR95 pKa = 11.84SRR97 pKa = 3.51

Molecular weight: 11.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

11851

29

1788

188.1

21.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.354 ± 0.998

0.616 ± 0.122

5.949 ± 0.343

7.645 ± 0.612

3.907 ± 0.253

5.78 ± 0.468

1.266 ± 0.186

6.902 ± 0.293

9.214 ± 0.419

8.295 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.455 ± 0.149

6.151 ± 0.274

2.371 ± 0.231

3.671 ± 0.232

3.578 ± 0.435

6.438 ± 0.478

6.278 ± 0.215

6.32 ± 0.21

1.004 ± 0.147

3.806 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski