Uncia uncia papillomavirus type 1
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4Z4U0|A4Z4U0_9PAPI Replication protein E1 OS=Uncia uncia papillomavirus type 1 OX=348686 GN=E1 PE=3 SV=1
MM1 pKa = 7.57 IGVAPTIGDD10 pKa = 3.31 IVLTEE15 pKa = 4.1 QPPEE19 pKa = 4.2 TVDD22 pKa = 3.44 LRR24 pKa = 11.84 CYY26 pKa = 9.66 EE27 pKa = 3.96 QMPGEE32 pKa = 4.11 EE33 pKa = 4.25 EE34 pKa = 4.39 EE35 pKa = 4.43 EE36 pKa = 4.38 VEE38 pKa = 4.33 SQSRR42 pKa = 11.84 DD43 pKa = 3.35 LYY45 pKa = 10.81 RR46 pKa = 11.84 VSAYY50 pKa = 10.47 CGLCGSGVRR59 pKa = 11.84 FACLAGGEE67 pKa = 4.14 DD68 pKa = 4.46 LLRR71 pKa = 11.84 SLLSSVQFVCVSCVNHH87 pKa = 6.03 QKK89 pKa = 10.67 LNHH92 pKa = 6.28 GGG94 pKa = 3.37
Molecular weight: 10.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.327
IPC2_protein 4.546
IPC_protein 4.393
Toseland 4.253
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.177
Rodwell 4.228
Grimsley 4.164
Solomon 4.317
Lehninger 4.266
Nozaki 4.444
DTASelect 4.533
Thurlkill 4.253
EMBOSS 4.19
Sillero 4.495
Patrickios 0.655
IPC_peptide 4.329
IPC2_peptide 4.495
IPC2.peptide.svr19 4.426
Protein with the highest isoelectric point:
>tr|A4Z4U2|A4Z4U2_9PAPI E4 (Fragment) OS=Uncia uncia papillomavirus type 1 OX=348686 GN=E4 PE=4 SV=1
MM1 pKa = 7.49 EE2 pKa = 4.93 NVSKK6 pKa = 10.77 ALEE9 pKa = 4.26 SVQEE13 pKa = 3.97 QLLALYY19 pKa = 9.53 EE20 pKa = 4.29 KK21 pKa = 10.7 DD22 pKa = 3.46 SAEE25 pKa = 3.71 LTEE28 pKa = 6.04 QIRR31 pKa = 11.84 HH32 pKa = 4.63 WNLNRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 3.95 QVLYY43 pKa = 10.79 YY44 pKa = 9.81 SARR47 pKa = 11.84 QQGITRR53 pKa = 11.84 IGMNPVPSLAAAQHH67 pKa = 5.91 KK68 pKa = 10.06 AKK70 pKa = 10.59 AAIEE74 pKa = 3.95 QEE76 pKa = 4.01 LLLRR80 pKa = 11.84 SLLDD84 pKa = 3.15 SGFAGEE90 pKa = 5.08 PWTLMDD96 pKa = 3.84 TSRR99 pKa = 11.84 EE100 pKa = 3.83 RR101 pKa = 11.84 LLADD105 pKa = 3.94 PPYY108 pKa = 9.84 CFKK111 pKa = 11.07 KK112 pKa = 10.47 GGQQVEE118 pKa = 4.26 VRR120 pKa = 11.84 FDD122 pKa = 3.45 NDD124 pKa = 3.19 RR125 pKa = 11.84 EE126 pKa = 4.29 NVSRR130 pKa = 11.84 YY131 pKa = 8.34 VLWTDD136 pKa = 4.09 IYY138 pKa = 10.66 HH139 pKa = 5.36 QTASDD144 pKa = 3.54 DD145 pKa = 3.26 WRR147 pKa = 11.84 KK148 pKa = 7.77 TRR150 pKa = 11.84 GQADD154 pKa = 3.43 NRR156 pKa = 11.84 GLYY159 pKa = 9.9 YY160 pKa = 10.48 VDD162 pKa = 3.26 EE163 pKa = 5.3 KK164 pKa = 11.26 GVKK167 pKa = 9.2 SYY169 pKa = 11.37 YY170 pKa = 10.45 VDD172 pKa = 4.83 FEE174 pKa = 4.27 QEE176 pKa = 3.59 AKK178 pKa = 10.75 KK179 pKa = 10.41 FGKK182 pKa = 8.02 TGHH185 pKa = 5.72 YY186 pKa = 10.37 AIVSKK191 pKa = 9.68 LTTPVPTSTTGSGLGDD207 pKa = 3.6 SSSSGAATASGAPKK221 pKa = 10.12 KK222 pKa = 8.23 QTPTKK227 pKa = 8.36 TRR229 pKa = 11.84 KK230 pKa = 9.4 RR231 pKa = 11.84 LLRR234 pKa = 11.84 LSSPKK239 pKa = 8.17 TTGSRR244 pKa = 11.84 FRR246 pKa = 11.84 RR247 pKa = 11.84 GGGGGGKK254 pKa = 9.12 RR255 pKa = 11.84 DD256 pKa = 4.14 LEE258 pKa = 4.49 TSTLRR263 pKa = 11.84 PVPPTPEE270 pKa = 4.05 EE271 pKa = 4.04 VGKK274 pKa = 10.21 LATSVPRR281 pKa = 11.84 GSGGRR286 pKa = 11.84 LGRR289 pKa = 11.84 LLHH292 pKa = 6.18 EE293 pKa = 5.0 ARR295 pKa = 11.84 DD296 pKa = 3.9 PPILVLKK303 pKa = 10.46 GDD305 pKa = 4.24 PNSLKK310 pKa = 9.42 CTRR313 pKa = 11.84 YY314 pKa = 9.34 RR315 pKa = 11.84 LKK317 pKa = 10.89 GKK319 pKa = 10.58 YY320 pKa = 9.69 SDD322 pKa = 3.9 LFCRR326 pKa = 11.84 ASTTWQWTSSTGTDD340 pKa = 2.18 RR341 pKa = 11.84 WGRR344 pKa = 11.84 SRR346 pKa = 11.84 MLLTFADD353 pKa = 4.23 RR354 pKa = 11.84 EE355 pKa = 4.15 QRR357 pKa = 11.84 DD358 pKa = 3.91 LFEE361 pKa = 4.69 KK362 pKa = 9.71 TVKK365 pKa = 10.32 LPKK368 pKa = 9.6 SVSYY372 pKa = 10.46 FRR374 pKa = 11.84 GFLEE378 pKa = 4.91 DD379 pKa = 3.63 LL380 pKa = 4.14
Molecular weight: 42.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.108
IPC2_protein 9.121
IPC_protein 9.107
Toseland 9.823
ProMoST 9.531
Dawson 10.058
Bjellqvist 9.721
Wikipedia 10.218
Rodwell 10.394
Grimsley 10.131
Solomon 10.072
Lehninger 10.043
Nozaki 9.838
DTASelect 9.721
Thurlkill 9.897
EMBOSS 10.233
Sillero 9.97
Patrickios 9.414
IPC_peptide 10.072
IPC2_peptide 8.287
IPC2.peptide.svr19 8.096
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2339
94
606
334.1
37.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.156 ± 0.645
2.522 ± 0.795
5.772 ± 0.437
6.541 ± 0.45
4.404 ± 0.731
6.883 ± 0.653
1.582 ± 0.177
3.378 ± 0.804
5.344 ± 0.924
10.261 ± 0.826
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.368 ± 0.287
3.206 ± 0.744
6.755 ± 1.364
4.703 ± 0.391
6.584 ± 0.767
7.824 ± 1.096
5.985 ± 0.57
6.712 ± 0.712
1.368 ± 0.378
2.651 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here