Spodoptera exigua multiple nucleopolyhedrovirus
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 135 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9J833|Q9J833_9ABAC ORF104 OS=Spodoptera exigua multiple nucleopolyhedrovirus OX=10454 GN=HTSEG24_101 PE=4 SV=1
MM1 pKa = 7.4 SSVSIANDD9 pKa = 3.04 VLNAILSDD17 pKa = 3.75 NLEE20 pKa = 5.13 LIDD23 pKa = 5.22 DD24 pKa = 4.31 SYY26 pKa = 11.78 IILNVVDD33 pKa = 4.76 HH34 pKa = 6.81 EE35 pKa = 4.9 SSGGTIKK42 pKa = 10.21 PVCIGEE48 pKa = 4.17 INSFQTNQSDD58 pKa = 4.15 KK59 pKa = 10.98 YY60 pKa = 8.88 PVSDD64 pKa = 3.58 SSVSSEE70 pKa = 4.05 LQSDD74 pKa = 3.58 QTLL77 pKa = 3.3
Molecular weight: 8.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.792
IPC2_protein 3.656
IPC_protein 3.567
Toseland 3.376
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.579
Rodwell 3.414
Grimsley 3.287
Solomon 3.554
Lehninger 3.503
Nozaki 3.732
DTASelect 3.948
Thurlkill 3.465
EMBOSS 3.579
Sillero 3.706
Patrickios 1.837
IPC_peptide 3.541
IPC2_peptide 3.668
IPC2.peptide.svr19 3.697
Protein with the highest isoelectric point:
>tr|Q9J891|Q9J891_9ABAC ORF44 OS=Spodoptera exigua multiple nucleopolyhedrovirus OX=10454 PE=4 SV=1
MM1 pKa = 7.43 WLISKK6 pKa = 9.66 KK7 pKa = 10.49 CQDD10 pKa = 3.03 INVSVFFDD18 pKa = 3.43 QFCVMWVSADD28 pKa = 3.77 DD29 pKa = 3.83 VLHH32 pKa = 6.85 LLRR35 pKa = 11.84 LPASTLQNIPQRR47 pKa = 11.84 HH48 pKa = 5.15 KK49 pKa = 10.79 KK50 pKa = 9.17 CWVDD54 pKa = 4.55 FRR56 pKa = 11.84 CPHH59 pKa = 6.33 HH60 pKa = 6.92 CSHH63 pKa = 7.74 DD64 pKa = 3.63 GGKK67 pKa = 9.99 VFIDD71 pKa = 4.44 LYY73 pKa = 11.61 GLGNLCNRR81 pKa = 11.84 VNSQIADD88 pKa = 3.48 YY89 pKa = 11.33 LMTVFVAEE97 pKa = 4.49 SYY99 pKa = 10.69 NEE101 pKa = 3.59 RR102 pKa = 11.84 RR103 pKa = 11.84 RR104 pKa = 11.84 DD105 pKa = 3.35 SRR107 pKa = 11.84 RR108 pKa = 11.84 SFSPRR113 pKa = 11.84 RR114 pKa = 11.84 RR115 pKa = 11.84 SHH117 pKa = 4.85 SPRR120 pKa = 11.84 RR121 pKa = 11.84 RR122 pKa = 11.84 SPSPRR127 pKa = 11.84 RR128 pKa = 11.84 RR129 pKa = 11.84 SPSPRR134 pKa = 11.84 RR135 pKa = 11.84 RR136 pKa = 11.84 SRR138 pKa = 11.84 SRR140 pKa = 11.84 SGGGGAGRR148 pKa = 11.84 RR149 pKa = 11.84 RR150 pKa = 11.84 SSSRR154 pKa = 11.84 CCPRR158 pKa = 11.84 RR159 pKa = 11.84 HH160 pKa = 5.82 HH161 pKa = 6.35 HH162 pKa = 6.13 HH163 pKa = 6.5 HH164 pKa = 6.37 HH165 pKa = 6.01 HH166 pKa = 6.37 HH167 pKa = 5.66 HH168 pKa = 6.44 TEE170 pKa = 3.54 LLEE173 pKa = 4.81 RR174 pKa = 11.84 ITRR177 pKa = 11.84 QNDD180 pKa = 4.15 LILTSVNQLTVTNANQHH197 pKa = 6.26 LEE199 pKa = 3.92 LSNILNALRR208 pKa = 11.84 LQQTTIAGQVAQILEE223 pKa = 4.42 TVEE226 pKa = 3.97 GLGDD230 pKa = 3.28 ITGDD234 pKa = 3.48 FTRR237 pKa = 11.84 LLAEE241 pKa = 4.63 LDD243 pKa = 3.43 TRR245 pKa = 11.84 LAALSTTILNAINQLSDD262 pKa = 3.16 QLRR265 pKa = 11.84 NEE267 pKa = 3.94 LTGINSVLNNLSSSVTNINATLNNLLQAINGLNLGTVVGEE307 pKa = 4.05 LQQTINTILDD317 pKa = 4.09 LLQTILGILQPNIPLNKK334 pKa = 9.73 KK335 pKa = 9.61
Molecular weight: 37.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.37
IPC_protein 10.248
Toseland 10.584
ProMoST 10.423
Dawson 10.657
Bjellqvist 10.438
Wikipedia 10.906
Rodwell 10.687
Grimsley 10.701
Solomon 10.833
Lehninger 10.804
Nozaki 10.628
DTASelect 10.409
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.613
Patrickios 10.409
IPC_peptide 10.847
IPC2_peptide 9.882
IPC2.peptide.svr19 8.658
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
135
0
135
39664
57
1222
293.8
33.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.115 ± 0.19
2.224 ± 0.129
7.319 ± 0.251
5.065 ± 0.116
4.856 ± 0.141
3.477 ± 0.153
2.327 ± 0.103
6.837 ± 0.133
6.593 ± 0.287
8.728 ± 0.198
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.753 ± 0.102
7.558 ± 0.168
3.515 ± 0.188
3.457 ± 0.179
5.206 ± 0.148
6.648 ± 0.199
5.695 ± 0.165
7.092 ± 0.153
0.711 ± 0.054
4.823 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here