Amino acid dipepetide frequency for Pseudoclostridium thermosuccinogenes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.865AlaAla: 5.865 ± 0.084	0.912AlaCys: 0.912 ± 0.027	3.687AlaAsp: 3.687 ± 0.06	4.709AlaGlu: 4.709 ± 0.072	3.023AlaPhe: 3.023 ± 0.053	5.276AlaGly: 5.276 ± 0.079	0.99AlaHis: 0.99 ± 0.032	5.382AlaIle: 5.382 ± 0.084	4.618AlaLys: 4.618 ± 0.062	6.732AlaLeu: 6.732 ± 0.094	1.972AlaMet: 1.972 ± 0.042	2.66AlaAsn: 2.66 ± 0.047	1.844AlaPro: 1.844 ± 0.039	1.876AlaGln: 1.876 ± 0.04	3.014AlaArg: 3.014 ± 0.054	4.003AlaSer: 4.003 ± 0.061	2.892AlaThr: 2.892 ± 0.051	5.613AlaVal: 5.613 ± 0.071	0.627AlaTrp: 0.627 ± 0.025	2.663AlaTyr: 2.663 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.689CysAla: 0.689 ± 0.023	0.231CysCys: 0.231 ± 0.015	0.728CysAsp: 0.728 ± 0.027	0.741CysGlu: 0.741 ± 0.025	0.507CysPhe: 0.507 ± 0.023	1.121CysGly: 1.121 ± 0.04	0.244CysHis: 0.244 ± 0.017	1.001CysIle: 1.001 ± 0.027	0.711CysLys: 0.711 ± 0.026	0.988CysLeu: 0.988 ± 0.031	0.334CysMet: 0.334 ± 0.018	0.526CysAsn: 0.526 ± 0.023	0.576CysPro: 0.576 ± 0.026	0.292CysGln: 0.292 ± 0.018	0.667CysArg: 0.667 ± 0.023	0.858CysSer: 0.858 ± 0.027	0.555CysThr: 0.555 ± 0.022	0.725CysVal: 0.725 ± 0.027	0.125CysTrp: 0.125 ± 0.01	0.463CysTyr: 0.463 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	3.676AspAla: 3.676 ± 0.052	0.671AspCys: 0.671 ± 0.026	3.036AspAsp: 3.036 ± 0.056	4.69AspGlu: 4.69 ± 0.062	2.747AspPhe: 2.747 ± 0.05	4.241AspGly: 4.241 ± 0.075	0.754AspHis: 0.754 ± 0.026	5.645AspIle: 5.645 ± 0.074	4.26AspLys: 4.26 ± 0.06	4.422AspLeu: 4.422 ± 0.065	1.871AspMet: 1.871 ± 0.038	2.595AspAsn: 2.595 ± 0.054	1.86AspPro: 1.86 ± 0.046	1.118AspGln: 1.118 ± 0.032	2.607AspArg: 2.607 ± 0.049	3.265AspSer: 3.265 ± 0.061	2.828AspThr: 2.828 ± 0.052	3.882AspVal: 3.882 ± 0.062	0.6AspTrp: 0.6 ± 0.023	2.641AspTyr: 2.641 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.938GluAla: 4.938 ± 0.063	0.651GluCys: 0.651 ± 0.025	3.841GluAsp: 3.841 ± 0.062	5.633GluGlu: 5.633 ± 0.08	2.806GluPhe: 2.806 ± 0.047	4.245GluGly: 4.245 ± 0.062	1.14GluHis: 1.14 ± 0.033	6.097GluIle: 6.097 ± 0.083	6.583GluLys: 6.583 ± 0.077	6.494GluLeu: 6.494 ± 0.083	2.125GluMet: 2.125 ± 0.043	4.059GluAsn: 4.059 ± 0.064	1.836GluPro: 1.836 ± 0.044	2.14GluGln: 2.14 ± 0.041	3.282GluArg: 3.282 ± 0.057	3.591GluSer: 3.591 ± 0.057	2.973GluThr: 2.973 ± 0.048	4.472GluVal: 4.472 ± 0.076	0.659GluTrp: 0.659 ± 0.026	3.14GluTyr: 3.14 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	2.893PheAla: 2.893 ± 0.045	0.588PheCys: 0.588 ± 0.024	2.737PheAsp: 2.737 ± 0.053	2.731PheGlu: 2.731 ± 0.051	2.005PhePhe: 2.005 ± 0.048	2.962PheGly: 2.962 ± 0.048	0.668PheHis: 0.668 ± 0.024	3.668PheIle: 3.668 ± 0.059	2.848PheLys: 2.848 ± 0.047	3.827PheLeu: 3.827 ± 0.064	1.195PheMet: 1.195 ± 0.037	2.285PheAsn: 2.285 ± 0.042	1.444PhePro: 1.444 ± 0.037	1.018PheGln: 1.018 ± 0.024	1.839PheArg: 1.839 ± 0.036	3.172PheSer: 3.172 ± 0.049	2.439PheThr: 2.439 ± 0.047	2.677PheVal: 2.677 ± 0.046	0.45PheTrp: 0.45 ± 0.019	1.807PheTyr: 1.807 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	4.259GlyAla: 4.259 ± 0.079	1.034GlyCys: 1.034 ± 0.032	3.642GlyAsp: 3.642 ± 0.055	4.412GlyGlu: 4.412 ± 0.071	3.273GlyPhe: 3.273 ± 0.062	4.536GlyGly: 4.536 ± 0.078	1.15GlyHis: 1.15 ± 0.032	6.545GlyIle: 6.545 ± 0.078	5.543GlyLys: 5.543 ± 0.072	5.863GlyLeu: 5.863 ± 0.078	2.291GlyMet: 2.291 ± 0.043	3.196GlyAsn: 3.196 ± 0.06	1.449GlyPro: 1.449 ± 0.036	1.784GlyGln: 1.784 ± 0.04	3.131GlyArg: 3.131 ± 0.056	4.204GlySer: 4.204 ± 0.063	3.682GlyThr: 3.682 ± 0.064	4.715GlyVal: 4.715 ± 0.067	0.76GlyTrp: 0.76 ± 0.03	3.301GlyTyr: 3.301 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	0.967HisAla: 0.967 ± 0.028	0.242HisCys: 0.242 ± 0.014	0.908HisAsp: 0.908 ± 0.028	1.01HisGlu: 1.01 ± 0.027	0.77HisPhe: 0.77 ± 0.027	1.2HisGly: 1.2 ± 0.034	0.38HisHis: 0.38 ± 0.019	1.44HisIle: 1.44 ± 0.034	0.93HisLys: 0.93 ± 0.028	1.363HisLeu: 1.363 ± 0.037	0.45HisMet: 0.45 ± 0.018	0.696HisAsn: 0.696 ± 0.024	0.794HisPro: 0.794 ± 0.028	0.438HisGln: 0.438 ± 0.017	0.696HisArg: 0.696 ± 0.024	0.965HisSer: 0.965 ± 0.027	0.789HisThr: 0.789 ± 0.024	0.985HisVal: 0.985 ± 0.028	0.197HisTrp: 0.197 ± 0.013	0.713HisTyr: 0.713 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.002IleAla: 6.002 ± 0.084	1.089IleCys: 1.089 ± 0.033	5.112IleAsp: 5.112 ± 0.063	5.526IleGlu: 5.526 ± 0.074	3.506IlePhe: 3.506 ± 0.06	5.279IleGly: 5.279 ± 0.073	1.258IleHis: 1.258 ± 0.032	6.878IleIle: 6.878 ± 0.084	6.128IleLys: 6.128 ± 0.066	7.467IleLeu: 7.467 ± 0.079	2.19IleMet: 2.19 ± 0.043	4.359IleAsn: 4.359 ± 0.055	3.605IlePro: 3.605 ± 0.053	2.061IleGln: 2.061 ± 0.039	3.75IleArg: 3.75 ± 0.058	6.181IleSer: 6.181 ± 0.075	4.429IleThr: 4.429 ± 0.063	5.256IleVal: 5.256 ± 0.063	0.682IleTrp: 0.682 ± 0.025	3.202IleTyr: 3.202 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	5.506LysAla: 5.506 ± 0.072	0.678LysCys: 0.678 ± 0.027	4.577LysAsp: 4.577 ± 0.063	6.05LysGlu: 6.05 ± 0.074	2.288LysPhe: 2.288 ± 0.04	4.838LysGly: 4.838 ± 0.069	1.205LysHis: 1.205 ± 0.029	5.635LysIle: 5.635 ± 0.066	5.911LysLys: 5.911 ± 0.072	6.53LysLeu: 6.53 ± 0.067	1.961LysMet: 1.961 ± 0.041	4.097LysAsn: 4.097 ± 0.072	2.538LysPro: 2.538 ± 0.048	2.131LysGln: 2.131 ± 0.046	3.329LysArg: 3.329 ± 0.058	4.218LysSer: 4.218 ± 0.059	3.579LysThr: 3.579 ± 0.063	4.912LysVal: 4.912 ± 0.065	0.668LysTrp: 0.668 ± 0.026	3.36LysTyr: 3.36 ± 0.054	0.0LysXaa: 0.0 ± 0.0
Leu	6.104LeuAla: 6.104 ± 0.086	1.157LeuCys: 1.157 ± 0.033	5.061LeuAsp: 5.061 ± 0.077	6.072LeuGlu: 6.072 ± 0.079	3.859LeuPhe: 3.859 ± 0.061	5.721LeuGly: 5.721 ± 0.077	1.344LeuHis: 1.344 ± 0.036	6.932LeuIle: 6.932 ± 0.081	7.477LeuLys: 7.477 ± 0.081	8.55LeuLeu: 8.55 ± 0.111	2.59LeuMet: 2.59 ± 0.049	4.472LeuAsn: 4.472 ± 0.057	3.597LeuPro: 3.597 ± 0.054	2.442LeuGln: 2.442 ± 0.051	3.778LeuArg: 3.778 ± 0.063	6.512LeuSer: 6.512 ± 0.076	4.497LeuThr: 4.497 ± 0.06	5.561LeuVal: 5.561 ± 0.07	0.836LeuTrp: 0.836 ± 0.028	3.527LeuTyr: 3.527 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.138MetAla: 2.138 ± 0.036	0.263MetCys: 0.263 ± 0.015	1.747MetAsp: 1.747 ± 0.039	2.166MetGlu: 2.166 ± 0.041	1.076MetPhe: 1.076 ± 0.029	1.91MetGly: 1.91 ± 0.036	0.488MetHis: 0.488 ± 0.021	1.987MetIle: 1.987 ± 0.04	2.26MetLys: 2.26 ± 0.041	2.908MetLeu: 2.908 ± 0.053	0.828MetMet: 0.828 ± 0.026	1.347MetAsn: 1.347 ± 0.035	1.216MetPro: 1.216 ± 0.027	0.886MetGln: 0.886 ± 0.027	1.223MetArg: 1.223 ± 0.029	1.751MetSer: 1.751 ± 0.036	1.335MetThr: 1.335 ± 0.031	1.867MetVal: 1.867 ± 0.046	0.22MetTrp: 0.22 ± 0.016	0.886MetTyr: 0.886 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.3AsnAla: 3.3 ± 0.053	0.59AsnCys: 0.59 ± 0.026	2.431AsnAsp: 2.431 ± 0.053	3.074AsnGlu: 3.074 ± 0.055	1.962AsnPhe: 1.962 ± 0.043	3.536AsnGly: 3.536 ± 0.054	0.751AsnHis: 0.751 ± 0.025	4.714AsnIle: 4.714 ± 0.072	3.141AsnLys: 3.141 ± 0.06	4.13AsnLeu: 4.13 ± 0.064	1.364AsnMet: 1.364 ± 0.032	2.439AsnAsn: 2.439 ± 0.054	2.342AsnPro: 2.342 ± 0.048	1.209AsnGln: 1.209 ± 0.029	2.311AsnArg: 2.311 ± 0.045	3.17AsnSer: 3.17 ± 0.057	2.581AsnThr: 2.581 ± 0.044	3.052AsnVal: 3.052 ± 0.048	0.505AsnTrp: 0.505 ± 0.022	2.073AsnTyr: 2.073 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	2.348ProAla: 2.348 ± 0.044	0.398ProCys: 0.398 ± 0.018	2.498ProAsp: 2.498 ± 0.05	3.409ProGlu: 3.409 ± 0.056	1.633ProPhe: 1.633 ± 0.037	2.553ProGly: 2.553 ± 0.048	0.61ProHis: 0.61 ± 0.022	2.295ProIle: 2.295 ± 0.045	2.001ProLys: 2.001 ± 0.044	2.836ProLeu: 2.836 ± 0.051	0.84ProMet: 0.84 ± 0.028	1.367ProAsn: 1.367 ± 0.032	0.977ProPro: 0.977 ± 0.033	1.034ProGln: 1.034 ± 0.029	1.219ProArg: 1.219 ± 0.029	2.021ProSer: 2.021 ± 0.04	1.582ProThr: 1.582 ± 0.039	3.132ProVal: 3.132 ± 0.05	0.388ProTrp: 0.388 ± 0.017	1.591ProTyr: 1.591 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	1.87GlnAla: 1.87 ± 0.038	0.261GlnCys: 0.261 ± 0.016	1.427GlnAsp: 1.427 ± 0.032	1.855GlnGlu: 1.855 ± 0.045	1.017GlnPhe: 1.017 ± 0.027	1.654GlnGly: 1.654 ± 0.04	0.446GlnHis: 0.446 ± 0.019	2.203GlnIle: 2.203 ± 0.041	2.317GlnLys: 2.317 ± 0.045	2.456GlnLeu: 2.456 ± 0.045	0.83GlnMet: 0.83 ± 0.028	1.428GlnAsn: 1.428 ± 0.035	0.822GlnPro: 0.822 ± 0.025	0.895GlnGln: 0.895 ± 0.03	1.273GlnArg: 1.273 ± 0.033	1.548GlnSer: 1.548 ± 0.036	1.241GlnThr: 1.241 ± 0.036	1.715GlnVal: 1.715 ± 0.036	0.345GlnTrp: 0.345 ± 0.017	1.215GlnTyr: 1.215 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	2.436ArgAla: 2.436 ± 0.049	0.525ArgCys: 0.525 ± 0.024	2.459ArgAsp: 2.459 ± 0.045	3.565ArgGlu: 3.565 ± 0.063	2.051ArgPhe: 2.051 ± 0.039	2.441ArgGly: 2.441 ± 0.047	0.753ArgHis: 0.753 ± 0.027	4.086ArgIle: 4.086 ± 0.065	3.729ArgLys: 3.729 ± 0.056	4.19ArgLeu: 4.19 ± 0.074	1.395ArgMet: 1.395 ± 0.033	2.325ArgAsn: 2.325 ± 0.044	1.3ArgPro: 1.3 ± 0.04	1.499ArgGln: 1.499 ± 0.036	2.161ArgArg: 2.161 ± 0.047	2.259ArgSer: 2.259 ± 0.048	2.124ArgThr: 2.124 ± 0.041	2.859ArgVal: 2.859 ± 0.053	0.456ArgTrp: 0.456 ± 0.021	2.121ArgTyr: 2.121 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	3.963SerAla: 3.963 ± 0.061	0.702SerCys: 0.702 ± 0.026	3.497SerAsp: 3.497 ± 0.054	3.889SerGlu: 3.889 ± 0.066	3.056SerPhe: 3.056 ± 0.052	5.157SerGly: 5.157 ± 0.074	0.967SerHis: 0.967 ± 0.031	5.486SerIle: 5.486 ± 0.071	4.389SerLys: 4.389 ± 0.065	5.811SerLeu: 5.811 ± 0.073	1.81SerMet: 1.81 ± 0.038	2.758SerAsn: 2.758 ± 0.047	2.076SerPro: 2.076 ± 0.04	1.665SerGln: 1.665 ± 0.037	2.975SerArg: 2.975 ± 0.052	4.179SerSer: 4.179 ± 0.075	3.038SerThr: 3.038 ± 0.053	4.214SerVal: 4.214 ± 0.057	0.659SerTrp: 0.659 ± 0.024	2.552SerTyr: 2.552 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.79ThrAla: 3.79 ± 0.059	0.525ThrCys: 0.525 ± 0.021	2.864ThrAsp: 2.864 ± 0.049	3.047ThrGlu: 3.047 ± 0.05	2.024ThrPhe: 2.024 ± 0.039	4.356ThrGly: 4.356 ± 0.062	0.821ThrHis: 0.821 ± 0.026	3.99ThrIle: 3.99 ± 0.06	2.71ThrLys: 2.71 ± 0.053	4.498ThrLeu: 4.498 ± 0.064	1.173ThrMet: 1.173 ± 0.03	2.012ThrAsn: 2.012 ± 0.045	2.071ThrPro: 2.071 ± 0.042	1.252ThrGln: 1.252 ± 0.032	1.992ThrArg: 1.992 ± 0.043	3.014ThrSer: 3.014 ± 0.053	2.556ThrThr: 2.556 ± 0.06	4.021ThrVal: 4.021 ± 0.07	0.521ThrTrp: 0.521 ± 0.022	1.982ThrTyr: 1.982 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	4.309ValAla: 4.309 ± 0.069	0.9ValCys: 0.9 ± 0.029	4.0ValAsp: 4.0 ± 0.057	4.604ValGlu: 4.604 ± 0.067	3.167ValPhe: 3.167 ± 0.057	4.06ValGly: 4.06 ± 0.072	1.057ValHis: 1.057 ± 0.031	5.633ValIle: 5.633 ± 0.073	4.944ValLys: 4.944 ± 0.061	6.457ValLeu: 6.457 ± 0.076	1.92ValMet: 1.92 ± 0.036	3.227ValAsn: 3.227 ± 0.058	2.496ValPro: 2.496 ± 0.045	1.662ValGln: 1.662 ± 0.036	3.01ValArg: 3.01 ± 0.058	4.578ValSer: 4.578 ± 0.064	3.484ValThr: 3.484 ± 0.068	4.777ValVal: 4.777 ± 0.074	0.626ValTrp: 0.626 ± 0.022	2.719ValTyr: 2.719 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.633TrpAla: 0.633 ± 0.022	0.121TrpCys: 0.121 ± 0.011	0.62TrpAsp: 0.62 ± 0.027	0.661TrpGlu: 0.661 ± 0.023	0.466TrpPhe: 0.466 ± 0.02	0.716TrpGly: 0.716 ± 0.03	0.187TrpHis: 0.187 ± 0.011	0.739TrpIle: 0.739 ± 0.027	0.703TrpLys: 0.703 ± 0.027	0.9TrpLeu: 0.9 ± 0.032	0.282TrpMet: 0.282 ± 0.015	0.576TrpAsn: 0.576 ± 0.024	0.274TrpPro: 0.274 ± 0.014	0.359TrpGln: 0.359 ± 0.018	0.422TrpArg: 0.422 ± 0.017	0.605TrpSer: 0.605 ± 0.025	0.475TrpThr: 0.475 ± 0.022	0.618TrpVal: 0.618 ± 0.019	0.151TrpTrp: 0.151 ± 0.011	0.422TrpTyr: 0.422 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.672TyrAla: 2.672 ± 0.049	0.544TyrCys: 0.544 ± 0.026	2.539TyrAsp: 2.539 ± 0.046	2.862TyrGlu: 2.862 ± 0.047	2.016TyrPhe: 2.016 ± 0.04	2.97TyrGly: 2.97 ± 0.049	0.717TyrHis: 0.717 ± 0.024	3.603TyrIle: 3.603 ± 0.056	2.807TyrLys: 2.807 ± 0.056	3.668TyrLeu: 3.668 ± 0.058	1.082TyrMet: 1.082 ± 0.028	2.247TyrAsn: 2.247 ± 0.051	1.617TyrPro: 1.617 ± 0.039	1.055TyrGln: 1.055 ± 0.028	2.054TyrArg: 2.054 ± 0.041	2.754TyrSer: 2.754 ± 0.054	2.14TyrThr: 2.14 ± 0.051	2.575TyrVal: 2.575 ± 0.051	0.455TyrTrp: 0.455 ± 0.019	1.966TyrTyr: 1.966 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3781 proteins (1216124 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---