Bacillus phage pGIL02

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; Betatectivirus; Bacillus virus Bam35

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160LKS9|A0A160LKS9_9VIRU Cytoplasmic protein OS=Bacillus phage pGIL02 OX=1768917 GN=ATN07_34675 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 5.03RR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 10.55ACEE8 pKa = 4.21DD9 pKa = 3.27EE10 pKa = 5.06LKK12 pKa = 10.92ALEE15 pKa = 4.49EE16 pKa = 3.86EE17 pKa = 4.59HH18 pKa = 6.92EE19 pKa = 4.23RR20 pKa = 11.84VPFLKK25 pKa = 8.24TTGMGKK31 pKa = 7.85TFLSINNLQFFAKK44 pKa = 9.98KK45 pKa = 10.07KK46 pKa = 9.08EE47 pKa = 4.05EE48 pKa = 4.07EE49 pKa = 4.14EE50 pKa = 4.07EE51 pKa = 4.36EE52 pKa = 4.05EE53 pKa = 4.15FEE55 pKa = 4.18EE56 pKa = 4.71VEE58 pKa = 4.16EE59 pKa = 4.31EE60 pKa = 4.59EE61 pKa = 4.44EE62 pKa = 4.83EE63 pKa = 4.13EE64 pKa = 4.88DD65 pKa = 4.46EE66 pKa = 4.71KK67 pKa = 11.21PKK69 pKa = 10.55PKK71 pKa = 10.2RR72 pKa = 11.84KK73 pKa = 9.13PKK75 pKa = 10.51SKK77 pKa = 10.65SEE79 pKa = 3.72EE80 pKa = 4.62DD81 pKa = 3.19APQWAKK87 pKa = 10.1TFMSTIQEE95 pKa = 4.13VIKK98 pKa = 10.57PKK100 pKa = 9.37EE101 pKa = 4.02QEE103 pKa = 3.62AGAQKK108 pKa = 10.74VPVPPKK114 pKa = 9.66PKK116 pKa = 10.07VEE118 pKa = 4.11EE119 pKa = 4.16EE120 pKa = 4.22EE121 pKa = 4.2EE122 pKa = 4.58PEE124 pKa = 4.11VEE126 pKa = 4.22EE127 pKa = 6.38LEE129 pKa = 4.96EE130 pKa = 4.53EE131 pKa = 4.24DD132 pKa = 4.34NKK134 pKa = 10.79PKK136 pKa = 10.59KK137 pKa = 10.15KK138 pKa = 10.69SFLSWFLL145 pKa = 3.44

Molecular weight: 17.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A161J814|A0A161J814_9VIRU Uncharacterized protein OS=Bacillus phage pGIL02 OX=1768917 GN=ATN07_34670 PE=4 SV=1
MM1 pKa = 7.32LTPRR5 pKa = 11.84EE6 pKa = 4.1QDD8 pKa = 3.18TLEE11 pKa = 4.79CISGYY16 pKa = 7.76MEE18 pKa = 4.24EE19 pKa = 5.09FGFAPSVRR27 pKa = 11.84DD28 pKa = 3.4MASRR32 pKa = 11.84MYY34 pKa = 10.48VSHH37 pKa = 6.29KK38 pKa = 8.35TAHH41 pKa = 6.76RR42 pKa = 11.84YY43 pKa = 9.33LMQLEE48 pKa = 4.4SKK50 pKa = 10.06GRR52 pKa = 11.84IRR54 pKa = 11.84RR55 pKa = 11.84VHH57 pKa = 4.8QRR59 pKa = 11.84PRR61 pKa = 11.84AIQLLL66 pKa = 3.95

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

4243

66

734

202.0

22.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.293 ± 0.489

0.778 ± 0.169

5.279 ± 0.305

7.942 ± 0.952

4.407 ± 0.257

7.353 ± 0.644

1.626 ± 0.156

5.303 ± 0.422

8.862 ± 0.9

7.66 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.875 ± 0.244

4.808 ± 0.441

4.384 ± 0.43

3.7 ± 0.493

4.69 ± 0.315

5.798 ± 0.457

6.033 ± 0.391

6.599 ± 0.461

1.343 ± 0.228

4.266 ± 0.595
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski